##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933216.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332478
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26384903662799	33.0	31.0	34.0	30.0	34.0
2	32.44698295827092	34.0	31.0	34.0	31.0	34.0
3	32.517315431396966	34.0	31.0	34.0	31.0	34.0
4	35.98092204596996	37.0	35.0	37.0	35.0	37.0
5	35.1964250266183	37.0	35.0	37.0	35.0	37.0
6	34.86517604172306	37.0	35.0	37.0	32.0	37.0
7	35.59429496086959	37.0	35.0	37.0	33.0	37.0
8	35.87393150825017	37.0	35.0	37.0	35.0	37.0
9	37.72912794229994	39.0	38.0	39.0	35.0	39.0
10	37.30183049705544	39.0	37.0	39.0	34.0	39.0
11	37.24351084883812	39.0	37.0	39.0	34.0	39.0
12	37.131310943882	39.0	37.0	39.0	34.0	39.0
13	37.099970524365524	39.0	37.0	39.0	33.0	39.0
14	38.305996186213825	40.0	38.0	41.0	33.0	41.0
15	38.39008295285703	40.0	38.0	41.0	34.0	41.0
16	38.3751135413471	40.0	38.0	41.0	34.0	41.0
17	38.32741414469529	40.0	38.0	41.0	34.0	41.0
18	38.26006833534851	40.0	37.0	41.0	34.0	41.0
19	38.26110900570865	40.0	37.0	41.0	34.0	41.0
20	38.185140069418125	40.0	37.0	41.0	34.0	41.0
21	38.0906676531981	40.0	37.0	41.0	34.0	41.0
22	38.097949338001314	40.0	37.0	41.0	34.0	41.0
23	38.008999091669224	40.0	37.0	41.0	33.0	41.0
24	37.94599041139564	40.0	37.0	41.0	33.0	41.0
25	37.828006665102656	40.0	36.0	41.0	33.0	41.0
26	37.7822863467658	40.0	36.0	41.0	33.0	41.0
27	37.73012951232864	40.0	36.0	41.0	33.0	41.0
28	37.62864911362556	40.0	36.0	41.0	33.0	41.0
29	37.54837011772207	40.0	36.0	41.0	33.0	41.0
30	37.461161339998434	40.0	36.0	41.0	33.0	41.0
31	37.39712101251812	40.0	36.0	41.0	32.0	41.0
32	37.34970734905768	40.0	36.0	41.0	32.0	41.0
33	37.22889935574685	39.0	36.0	41.0	32.0	41.0
34	37.10943882001215	39.0	35.0	41.0	32.0	41.0
35	36.96739032357028	39.0	35.0	41.0	31.0	41.0
36	36.84620335781616	39.0	35.0	41.0	31.0	41.0
37	36.793631458322054	39.0	35.0	41.0	31.0	41.0
38	36.69292404309458	39.0	35.0	41.0	31.0	41.0
39	36.62212537370894	39.0	35.0	41.0	31.0	41.0
40	36.52177286918232	39.0	35.0	41.0	30.0	41.0
41	36.40708257388459	39.0	35.0	41.0	30.0	41.0
42	36.316920217277534	39.0	35.0	41.0	30.0	41.0
43	36.17338590824055	39.0	35.0	40.0	30.0	41.0
44	36.0764952869062	38.0	35.0	40.0	30.0	41.0
45	36.06334253694981	38.0	35.0	40.0	30.0	41.0
46	35.882329056358614	38.0	35.0	40.0	29.0	41.0
47	35.808823440949475	38.0	35.0	40.0	29.0	41.0
48	35.700298967149706	38.0	35.0	40.0	29.0	41.0
49	35.728009071276894	38.0	35.0	40.0	29.0	41.0
50	35.534309036988915	38.0	34.0	40.0	28.0	41.0
51	34.482901124284915	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	13.0
11	7.0
12	13.0
13	4.0
14	9.0
15	14.0
16	21.0
17	51.0
18	93.0
19	164.0
20	270.0
21	426.0
22	688.0
23	963.0
24	1300.0
25	1902.0
26	2654.0
27	3122.0
28	3694.0
29	4276.0
30	5300.0
31	6889.0
32	8877.0
33	12212.0
34	20134.0
35	24979.0
36	28983.0
37	43335.0
38	70088.0
39	91947.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.518313993707856	4.270658509735982	53.97890988275916	17.232117613797005
2	21.18786806946625	4.234265124308976	52.51114359446339	22.06672321176138
3	21.3250200013234	4.252612202912674	52.03802958391231	22.384338211851613
4	18.917642671094026	4.996120044032989	50.93209174742389	25.154145537449097
5	17.28836193672965	7.786680622477276	50.06376361744236	24.861193823350717
6	22.279068088715643	7.619752284361672	52.940044153297364	17.161135473625322
7	83.54778361275031	2.097582396429237	9.774481319064721	4.580152671755725
8	85.83966457931052	2.6025782157014903	7.457937066512671	4.0998201384753274
9	79.86964551037964	5.459007814050854	9.40513357274767	5.26621310282184
10	36.80814971216141	28.921612858595154	17.34099699829763	16.929240430945807
11	28.624751111351728	25.18873429219377	25.58184300916151	20.604671587292994
12	25.755689098226046	21.41495076365955	32.3398841427102	20.48947599540421
13	23.970909353400828	21.872725413410812	33.35528967330169	20.80107555988667
14	21.23298383652452	24.619072540137992	31.762703096144705	22.385240527192778
15	19.77634610410313	23.56576976521755	34.11533996234337	22.54254416833595
16	22.028825967432432	22.308242951413327	32.04633088505104	23.616600196103203
17	22.32929697604052	21.8730261851912	30.043792371224566	25.75388446754372
18	23.005732710134204	21.938895205096276	30.7475983373336	24.30777374743592
19	24.154981682998574	23.768189173418993	29.16283182646671	22.91399731711572
20	26.678456920457894	23.312820697910837	28.447596532702917	21.56112584892835
21	26.676050746214784	23.443957194160213	27.795824084601083	22.08416797502391
22	24.767653799649903	23.447867227305267	27.08991271602933	24.694566257015502
23	23.81059799445377	24.634111129157418	27.279098165893682	24.27619271049513
24	24.087909575971945	23.24965862402926	28.564295983493643	24.098135816505152
25	23.55103194797851	24.45846040941055	27.116681404483906	24.873826238127034
26	23.23762775281372	24.96736626182785	27.495052304212606	24.29995368114582
27	22.930539765037086	23.993467236929963	28.647308994880866	24.42868400315209
28	22.004463453220964	24.41394618591305	28.961013961826044	24.620576399039937
29	22.681500730875428	24.72223726081124	28.202166759905918	24.394095248407414
30	23.94053140358159	23.803680243504832	28.6385866132496	23.61720173966398
31	23.40335300380777	25.935851394678743	26.71575262122607	23.94504298028742
32	23.301992913816854	25.787570906947227	26.46551049994285	24.444925679293068
33	24.056328539031156	24.703288638646768	27.167812607149948	24.07257021517213
34	22.61142090604491	23.99948267253773	28.25540336503468	25.13369305638268
35	23.259884864562466	24.186562719939364	27.83642827495353	24.717124140544637
36	24.75802910267747	24.89638412165617	27.167812607149948	23.177774168516414
37	23.975721701887043	26.13165382371164	27.111568284217302	22.781056190184014
38	22.70466015796534	25.89975878103213	27.297445244497382	24.098135816505152
39	23.201234367386714	25.4723620811001	28.00245429772797	23.323949253785212
40	23.24965862402926	23.897821810766427	30.122293805905954	22.73022575929836
41	21.46638273810598	24.139341550418372	29.001016608617714	25.393259102857936
42	23.211159836139533	24.321910021114178	27.28932440642689	25.177605736319396
43	23.683070759569055	23.837065911127954	27.880641726670635	24.599221602632355
44	23.00092036164799	23.964593146012668	28.191639747592323	24.84284674474702
45	23.221386076672744	23.55373889400201	27.745595197276213	25.47927983204904
46	22.450808775317466	24.263259523938427	29.42991716745168	23.856014533292427
47	21.614963997617885	25.01518897490962	29.488266892847047	23.881580134625448
48	21.46277347674132	23.862631512460975	29.51653944020356	25.158055570594147
49	22.444793339709694	23.709237904462853	29.840169875901562	24.00579887992589
50	21.873928500532365	23.580206810676195	29.01725828475869	25.52860640403275
51	21.245917023081226	23.32214462310288	28.394059155793766	27.037879198022125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	197.5
2	329.0
3	1201.5
4	2074.0
5	1404.5
6	735.0
7	737.0
8	739.0
9	769.0
10	799.0
11	833.0
12	867.0
13	864.0
14	861.0
15	902.0
16	943.0
17	865.5
18	788.0
19	846.0
20	904.0
21	971.0
22	1038.0
23	1163.5
24	1289.0
25	1557.5
26	2231.5
27	2637.0
28	3246.5
29	3856.0
30	4292.5
31	4729.0
32	5565.5
33	6402.0
34	7351.5
35	8301.0
36	8929.0
37	9557.0
38	10377.0
39	11197.0
40	12448.0
41	13699.0
42	15166.5
43	16634.0
44	18321.0
45	20008.0
46	22291.0
47	24574.0
48	27827.0
49	31080.0
50	30193.0
51	29306.0
52	26595.0
53	23884.0
54	21980.0
55	20076.0
56	19638.0
57	19200.0
58	18725.5
59	18251.0
60	16787.5
61	15324.0
62	13938.0
63	12552.0
64	11032.0
65	9512.0
66	8123.5
67	6735.0
68	5516.5
69	4298.0
70	3647.5
71	2997.0
72	2401.0
73	1805.0
74	1513.5
75	962.0
76	702.0
77	519.5
78	337.0
79	271.5
80	206.0
81	150.0
82	94.0
83	57.5
84	21.0
85	17.5
86	14.0
87	10.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	332478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.12588393576035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.63320950309426	31.422601301111165
2	8.564086094679183	7.729239102487038
3	5.254067998304066	7.112833880461859
4	4.101623723641899	7.403575844049022
5	3.2967494876756334	7.4384367373064
6	2.579303928631251	6.983602183107871
7	2.0046409205770566	6.332283546038522
8	1.4274186906248612	5.1530824128698
9	0.9949651579360647	4.040881401343349
>10	2.083794197299765	12.423605166393353
>50	0.03942530616242125	1.2242632656728794
>100	0.017373863732592417	1.3472926775927156
>500	0.0013364510563532628	0.41371655184807654
>1k	0.002004676584529894	0.9745859297179178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1124	0.338067481156648	No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT	1103	0.33175127376848995	No Hit
TCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	1005	0.3022756392904192	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	757	0.22768423775407698	No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	615	0.18497464493891325	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTG	345	0.10376626423402449	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3519029830545179	0.0
2	0.0	0.0	0.0	1.1414289065742695	0.0
3	0.0	0.0	0.0	1.6635687173286653	0.0
4	0.0	0.0	0.0	2.3348913311557458	0.0
5	0.0	0.0	0.0	3.248635999975938	0.0
6	0.0	0.0	0.0	3.9028146223208755	0.0
7	0.0	0.0	0.0	4.465859395208104	0.0
8	0.0	0.0	0.0	5.262303069676791	0.0
9	0.0	0.0	0.0	5.813016199568092	0.0
10	0.0	0.0	0.0	6.601639807746678	0.0
11	0.0	0.0	0.0	7.886536853566251	0.0
12	3.0077178038847685E-4	0.0	0.0	8.884497620895218	0.0
13	3.0077178038847685E-4	0.0	0.0	9.244220670239836	0.0
14	3.0077178038847685E-4	0.0	0.0	9.384981863461643	0.0
15	3.0077178038847685E-4	0.0	0.0	9.589506674125808	0.0
16	3.0077178038847685E-4	0.0	0.0	10.093600178056894	0.0
17	3.0077178038847685E-4	0.0	0.0	10.817557853451957	0.0
18	3.0077178038847685E-4	0.0	0.0	11.604376830948214	0.0
19	3.0077178038847685E-4	0.0	0.0	12.069971546989576	0.0
20	3.0077178038847685E-4	0.0	0.0	12.54458941644259	0.0
21	3.0077178038847685E-4	0.0	0.0	13.058006845565721	0.0
22	6.015435607769537E-4	0.0	0.0	13.59729064780226	0.0
23	6.015435607769537E-4	0.0	0.0	14.110407305145001	0.0
24	6.015435607769537E-4	0.0	0.0	14.519156154692942	0.0
25	9.023153411654305E-4	0.0	0.0	14.868652963504353	0.0
26	9.023153411654305E-4	0.0	0.0	15.19679497590818	0.0
27	9.023153411654305E-4	0.0	0.0	15.526440847213951	0.0
28	9.023153411654305E-4	0.0	0.0	15.853680544276614	0.0
29	9.023153411654305E-4	0.0	0.0	16.18994339475093	0.0
30	9.023153411654305E-4	0.0	0.0	16.584856742401	0.0
31	9.023153411654305E-4	0.0	0.0	16.961122239666985	0.0
32	9.023153411654305E-4	0.0	0.0	17.29197119809431	0.0
33	9.023153411654305E-4	0.0	0.0	17.6315425381529	0.0
34	9.023153411654305E-4	0.0	0.0	18.015628101708984	0.0
35	9.023153411654305E-4	0.0	0.0	18.384675076245646	0.0
36	9.023153411654305E-4	0.0	0.0	18.730863395472785	0.0
37	9.023153411654305E-4	0.0	0.0	19.079758660723417	0.0
38	9.023153411654305E-4	0.0	0.0	19.42624775173094	0.0
39	9.023153411654305E-4	0.0	0.0	19.776646875883518	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGCG	20	7.0260465E-4	45.0	1
TACGGGA	95	0.0	45.0	4
GCGATGA	20	7.0260465E-4	45.0	9
TTACGGG	85	0.0	42.35294	3
ACGGGAC	60	3.6379788E-12	41.249996	5
TATGGGA	150	0.0	40.500004	4
TATCGGG	50	1.0768417E-9	40.500004	3
CGAATAT	45	1.9212166E-8	40.0	14
GCCGATT	80	0.0	39.375	9
CTACGGG	40	3.4483492E-7	39.375	3
TTTGGGC	960	0.0	38.67187	4
CGGGATA	35	6.234899E-6	38.57143	6
TGGGCGA	600	0.0	38.250004	6
TTTGGGA	1800	0.0	38.25	4
TTGGGAC	730	0.0	37.910957	5
GCGATCT	155	0.0	37.741936	9
TAACGGG	30	1.1381775E-4	37.499996	3
GGGCGAT	895	0.0	36.955307	7
GTGGCGT	55	2.737579E-9	36.81818	34
GGCGATG	325	0.0	36.692307	8
>>END_MODULE