FastQCFastQC Report
Sat 14 Jan 2017
SRR2933215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775717
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66420.3740460895514319No Hit
CCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC29950.16866426350595282TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGCT28200.15880908951144804Illumina PCR Primer Index 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC26690.15030548223618967TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC22240.1252451826501633TruSeq Adapter, Index 21 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19500.10981479593876727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG406.8193913E-945.01
CGTTTTT42000.039.2678571
TTGGGAC45800.038.3187755
CGAATAT3550.038.0281714
ACAATCG301.1401725E-437.49999625
ATCGAGA301.1401725E-437.49999610
TTTGGGC42450.037.208484
AGCTACG3650.036.98639
TTTGGGA120050.036.9408574
TACGGGA4150.036.867474
CGTTTGG11800.036.8008462
TTGGGAT71350.036.7694475
GCTACGA3800.036.7105310
TTACGGG6100.036.147543
TTGGGCA20200.036.0891085
CTATGCG504.88144E-836.01
TCTTGCG6000.036.01
TGCGTAG250.00210741736.01
TACGAAT3800.035.52631812
TGGGCGA30850.035.3727726
Produced by FastQC (version 0.11.5)