FastQCFastQC Report
Sat 14 Jan 2017
SRR2933214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2952375
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379141.2841864600533468No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT103650.3510732884542106No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT97660.3307845378720522No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT90450.30636352089419533No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC87340.2958296286887675No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC86110.29166349125703883No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC78730.26666666666666666No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC78280.2651424700453025No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC76040.257555357974512No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC72540.24570049536390193No Hit
CGTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT58670.1987213683898556No Hit
CGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG52030.17623100046572673No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTC48340.16373258817054065No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG43700.1480164274524747No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC43280.1465938439392015No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGT40540.1373131800668953No Hit
TCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG35490.12020830687158644No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG32770.11099538507134087No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC31580.10696473178373343No Hit
TTCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT31420.1064227952072484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT314550.043.912731
CGTTATT8450.043.6686361
GTTTTTT352600.039.1101842
CGTTTCT12650.038.7747041
TTGGGAC48750.037.8923075
TTACGGG7400.037.7027053
TGGGACG4000.037.6875046
CGTTTTC12150.037.592591
CGTTTAT4850.037.11341
GCGATCG1950.036.923089
TGGGCGA44900.036.881966
TTTGGGC96150.036.8564764
AGGGCGA5050.036.534656
TCGTTTG10300.036.4805831
TCTAGCG1050.036.428571
TTGGGCG39950.036.2140165
ACACGAC3050.036.14753726
TCAAGCG3300.036.13636417
CGTTTGG18600.036.0483862
TTCCGCA250.002107642636.0000041
Produced by FastQC (version 0.11.5)