##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933214.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2952375
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03379076167492	33.0	31.0	34.0	30.0	34.0
2	32.26492196960074	34.0	31.0	34.0	30.0	34.0
3	32.242306617553666	33.0	31.0	34.0	30.0	34.0
4	35.86300520767179	37.0	35.0	37.0	35.0	37.0
5	35.0751706676828	37.0	35.0	37.0	35.0	37.0
6	34.83309708285702	37.0	35.0	37.0	32.0	37.0
7	35.635649265421904	37.0	35.0	37.0	33.0	37.0
8	35.881749777721325	37.0	35.0	37.0	35.0	37.0
9	37.74017629874254	39.0	38.0	39.0	35.0	39.0
10	37.1953976036242	39.0	37.0	39.0	34.0	39.0
11	37.16536822050045	39.0	37.0	39.0	34.0	39.0
12	37.173581269317076	39.0	37.0	39.0	34.0	39.0
13	37.20764875735637	39.0	37.0	39.0	34.0	39.0
14	38.40370244294847	40.0	38.0	41.0	34.0	41.0
15	38.44902934078496	40.0	38.0	41.0	34.0	41.0
16	38.41819314958296	40.0	38.0	41.0	34.0	41.0
17	38.34314170794699	40.0	38.0	41.0	34.0	41.0
18	38.15672162242262	39.0	38.0	41.0	33.0	41.0
19	38.0053140268428	39.0	37.0	41.0	34.0	41.0
20	37.76615301240527	39.0	35.0	41.0	34.0	41.0
21	37.651376942292224	39.0	35.0	41.0	33.0	41.0
22	37.70795579829798	39.0	35.0	41.0	33.0	41.0
23	37.62717134510352	39.0	35.0	41.0	33.0	41.0
24	37.55742309157881	39.0	35.0	41.0	33.0	41.0
25	37.45612566154367	39.0	35.0	41.0	33.0	41.0
26	37.424283839281934	39.0	35.0	41.0	33.0	41.0
27	37.39777806003641	39.0	35.0	41.0	33.0	41.0
28	37.29389423769	39.0	35.0	41.0	33.0	41.0
29	37.197757059994075	39.0	35.0	41.0	33.0	41.0
30	37.035310555061606	39.0	35.0	41.0	32.0	41.0
31	36.84401744358355	39.0	35.0	41.0	31.0	41.0
32	36.57578288665905	39.0	35.0	41.0	30.0	41.0
33	36.136694017528264	39.0	35.0	41.0	29.0	41.0
34	35.749289639696855	39.0	35.0	41.0	26.0	41.0
35	35.46426250052924	39.0	35.0	41.0	24.0	41.0
36	35.28539667217071	39.0	35.0	41.0	23.0	41.0
37	35.238214996401204	39.0	35.0	41.0	23.0	41.0
38	35.1697850035988	39.0	35.0	41.0	23.0	41.0
39	35.09202658876328	39.0	35.0	40.0	22.0	41.0
40	35.0049820906897	39.0	35.0	40.0	22.0	41.0
41	34.87111359498709	39.0	35.0	40.0	21.0	41.0
42	34.79044972268089	39.0	34.0	40.0	21.0	41.0
43	34.67564316863542	38.0	34.0	40.0	20.0	41.0
44	34.61803293958254	38.0	34.0	40.0	19.0	41.0
45	34.64932063169482	38.0	34.0	40.0	20.0	41.0
46	34.44620585122147	38.0	34.0	40.0	19.0	41.0
47	34.379644184766505	38.0	34.0	40.0	19.0	41.0
48	34.28527372030992	38.0	34.0	40.0	18.0	41.0
49	34.35560794275795	38.0	34.0	40.0	19.0	41.0
50	34.168920276048944	38.0	34.0	40.0	18.0	41.0
51	33.19779736652695	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	16.0
10	18.0
11	24.0
12	22.0
13	34.0
14	26.0
15	69.0
16	131.0
17	326.0
18	825.0
19	1831.0
20	3330.0
21	5483.0
22	8717.0
23	14577.0
24	25606.0
25	46660.0
26	66697.0
27	65500.0
28	56093.0
29	50935.0
30	53825.0
31	63097.0
32	78990.0
33	103468.0
34	153896.0
35	201605.0
36	247237.0
37	361699.0
38	606501.0
39	734927.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.343494644142428	3.955425716584106	51.129311147804735	23.571768491468735
2	28.131961556374108	4.596197976205597	46.359727338159956	20.91211312926034
3	16.67294974385029	4.658893263897709	57.123299038909344	21.544857953342646
4	15.120199839112578	3.875794910876836	57.64206782674965	23.361937423260933
5	15.135712773614463	6.932215589144334	56.376374952368856	21.55569668487235
6	17.597595156441848	7.04504001016131	58.8989881027986	16.45837673059825
7	72.9747406748804	1.4499513103857065	20.43287184046742	5.14243617426648
8	74.9097252212202	2.1959947499894152	17.628248444049284	5.266031584741098
9	68.64839324272832	4.451907362716457	20.312866759812014	6.586832634743216
10	30.62490367924129	20.488454210593165	30.767162030568613	18.119480079596933
11	22.751615225030697	23.91488208645582	33.429391591515305	19.90411109699818
12	17.980676573944706	20.48337355518862	40.60663025530293	20.92931961556374
13	18.59187941911173	21.015182691900588	42.45724205089123	17.935695838096446
14	17.496998179431813	23.551513611922605	40.417562132181715	18.533926076463864
15	16.14225835132732	23.96982090689699	41.53239341208349	18.355527329692197
16	16.405605656463017	23.10776916888945	39.84903679241289	20.637588382234643
17	16.845454930352684	24.4200008467759	37.55322409924213	21.181320123629284
18	17.6452517041365	23.47550700707058	38.01542825691181	20.86381303188111
19	18.446945255938015	26.41690164697913	35.38165036623057	19.75450273085228
20	19.459994072568694	25.38989796350396	35.666167068885215	19.483940895042124
21	19.473982810449215	26.164968880985644	35.52045387188281	18.84059443668233
22	18.33859181167704	23.801244760574114	35.16856767856387	22.691595749184977
23	17.68054532368009	24.949032558533386	34.582361657987214	22.788060459799315
24	18.2836868622719	23.853236800880648	35.769439857741645	22.093636479105804
25	18.2713916761929	25.67620983106821	33.86168762437021	22.190710868368686
26	17.93478132012363	26.06630255302934	34.88140903509886	21.11750709174817
27	18.857326728481308	24.336508742961175	35.03223675854185	21.773927770015668
28	17.988094330835345	23.93398535077692	35.30774376561243	22.770176552775307
29	18.072026758118465	23.47388119734112	34.19528345823278	24.258808586307634
30	18.45399043143232	24.42643634362166	34.37358059189635	22.745992633049664
31	17.184334645835982	25.379906007875014	32.804369363647915	24.631389982641096
32	16.47710741352301	25.202963715652654	32.223379482619926	26.09654938820441
33	17.079707015538336	23.93859181167704	32.005554849908975	26.97614632287565
34	16.226427875862655	25.369676954993857	30.448842033955714	27.955053135187775
35	15.962572505186504	25.823989161268468	30.168288242516617	28.045150091028408
36	16.48554130149456	25.790931030102882	30.83802023794403	26.885507430458528
37	16.12508573605995	26.14373174139464	32.27848765824125	25.45269486430416
38	16.61381091494136	24.850095262288836	30.97157373301156	27.564520089758243
39	17.99175240272662	24.157635801685085	31.213887124772427	26.63672467081587
40	18.70436512976841	24.91529700664719	31.52678775562047	24.85355010796393
41	17.26318641771455	25.72975993903214	31.92826114568779	25.078792497565523
42	18.318878868707397	25.247233159744276	32.74628053685592	23.68760743469241
43	18.92554299504636	23.825699648588	33.71143570853973	23.537321647825905
44	19.193022566577756	24.03319361530971	30.904678436851686	25.869105381260848
45	19.553782971336638	23.42954401117744	29.88709090139295	27.129582116092976
46	19.96324992590711	24.13324865574326	30.198568948727715	25.704932469621916
47	18.140784961260003	24.29935221643592	32.14232609339938	25.417536728904693
48	17.74005673398535	23.171785426986748	32.4674880392904	26.620669799737502
49	18.618027858927135	21.92976840679114	33.03459079554596	26.41761293873576
50	18.143460773106398	21.475456200516533	32.629052881155005	27.75203014522207
51	17.060874719505485	21.426139972056397	31.610720182903595	29.902265125534527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	315.0
1	1134.0
2	1953.0
3	31787.5
4	61622.0
5	42506.5
6	23391.0
7	23708.5
8	24026.0
9	24682.5
10	25339.0
11	25608.0
12	25877.0
13	25791.5
14	25706.0
15	25173.5
16	24641.0
17	23842.0
18	23043.0
19	22724.0
20	22405.0
21	21628.5
22	20852.0
23	21475.5
24	22099.0
25	22844.0
26	25863.0
27	28137.0
28	31344.0
29	34551.0
30	39397.5
31	44244.0
32	50838.0
33	57432.0
34	66012.0
35	74592.0
36	84088.5
37	93585.0
38	102858.5
39	112132.0
40	126376.5
41	140621.0
42	158661.5
43	176702.0
44	201983.5
45	227265.0
46	281660.5
47	336056.0
48	344549.5
49	353043.0
50	325464.5
51	297886.0
52	246433.5
53	194981.0
54	163217.5
55	131454.0
56	112637.0
57	93820.0
58	82642.5
59	71465.0
60	63033.5
61	54602.0
62	45530.0
63	36458.0
64	30778.0
65	25098.0
66	20685.0
67	16272.0
68	13284.0
69	10296.0
70	8335.0
71	6374.0
72	5349.5
73	4325.0
74	3624.0
75	2307.5
76	1692.0
77	1220.5
78	749.0
79	612.5
80	476.0
81	344.0
82	212.0
83	125.5
84	39.0
85	28.5
86	18.0
87	13.0
88	8.0
89	5.5
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2952375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.181862076400805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77546103802254	28.932482021294593
2	9.468681860473055	7.230638096838006
3	3.4801153042348307	3.986318476687978
4	1.8717772281111753	2.8587175984595476
5	1.2946834503693871	2.4716712467301316
6	0.9994601997220736	2.289675089798412
7	0.8345779698921625	2.230601865689759
8	0.667953244267842	2.0402958916895355
9	0.6079050329532194	2.0889851511392736
>10	4.819824364786863	30.959925266709508
>50	0.1122189447180551	2.977251290168749
>100	0.05837925043072848	4.13215331640724
>500	0.005108403850263551	1.3134283567278904
>1k	0.0027782547255705134	2.17407074006929
>5k	8.962112017969399E-4	2.6617258871830782
>10k+	1.7924224035938797E-4	1.6520597044071716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37914	1.2841864600533468	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	10365	0.3510732884542106	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT	9766	0.3307845378720522	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT	9045	0.30636352089419533	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	8734	0.2958296286887675	No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	8611	0.29166349125703883	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	7873	0.26666666666666666	No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	7828	0.2651424700453025	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC	7604	0.257555357974512	No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	7254	0.24570049536390193	No Hit
CGTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT	5867	0.1987213683898556	No Hit
CGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	5203	0.17623100046572673	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTC	4834	0.16373258817054065	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG	4370	0.1480164274524747	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC	4328	0.1465938439392015	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGT	4054	0.1373131800668953	No Hit
TCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	3549	0.12020830687158644	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG	3277	0.11099538507134087	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC	3158	0.10696473178373343	No Hit
TTCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT	3142	0.1064227952072484	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7258139633346033E-4	0.0	0.0	0.3929717600237097	0.0
2	3.7258139633346033E-4	0.0	0.0	1.3146026504085693	0.0
3	3.7258139633346033E-4	0.0	0.0	2.034700876413057	0.0
4	3.7258139633346033E-4	0.0	0.0	3.1178966086625173	0.0
5	4.064524323637749E-4	0.0	0.0	5.062060205766544	0.0
6	4.064524323637749E-4	0.0	0.0	7.284474363859604	0.0
7	4.064524323637749E-4	0.0	0.0	9.067208603243152	0.0
8	4.064524323637749E-4	0.0	0.0	11.246030737965198	0.0
9	4.064524323637749E-4	0.0	0.0	12.604597993141116	0.0
10	4.064524323637749E-4	0.0	0.0	14.400643549684576	0.0
11	4.064524323637749E-4	0.0	0.0	16.617875439265	0.0
12	4.064524323637749E-4	0.0	0.0	18.52708412718574	0.0
13	4.064524323637749E-4	0.0	0.0	19.29880181210043	0.0
14	4.064524323637749E-4	0.0	0.0	19.62206697997375	0.0
15	4.064524323637749E-4	0.0	0.0	19.95566281383632	0.0
16	4.064524323637749E-4	0.0	0.0	20.571438248867437	0.0
17	4.064524323637749E-4	0.0	0.0	21.43426902070367	0.0
18	4.064524323637749E-4	0.0	0.0	22.39336127693806	0.0
19	4.064524323637749E-4	0.0	0.0	23.07088361065244	0.0
20	4.064524323637749E-4	0.0	0.0	23.735196240315002	0.0
21	4.064524323637749E-4	0.0	0.0	24.610796392734663	0.0
22	4.064524323637749E-4	0.0	0.0	25.55058215843177	0.0
23	4.064524323637749E-4	0.0	0.0	26.482611456877937	0.0
24	4.064524323637749E-4	0.0	0.0	27.21209195986282	0.0
25	4.064524323637749E-4	0.0	0.0	27.859333587366102	0.0
26	4.064524323637749E-4	0.0	0.0	28.42105084889284	0.0
27	4.064524323637749E-4	0.0	0.0	28.987950378932215	0.0
28	4.064524323637749E-4	0.0	0.0	29.586485456623905	0.0
29	4.064524323637749E-4	0.0	0.0	30.216723824039967	0.0
30	4.064524323637749E-4	0.0	0.0	30.907489732842205	0.0
31	4.064524323637749E-4	0.0	0.0	31.543858757779752	0.0
32	4.064524323637749E-4	0.0	0.0	32.18039713789746	0.0
33	4.064524323637749E-4	0.0	0.0	32.78529997036284	0.0
34	4.064524323637749E-4	0.0	0.0	33.38038020237944	0.0
35	4.064524323637749E-4	0.0	0.0	33.996799187095135	0.0
36	4.064524323637749E-4	0.0	0.0	34.590118125238156	0.0
37	4.064524323637749E-4	0.0	0.0	35.19403869765866	0.0
38	4.403234683940895E-4	0.0	0.0	35.80517380075363	0.0
39	4.403234683940895E-4	0.0	0.0	36.39232821033914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	31455	0.0	43.91273	1
CGTTATT	845	0.0	43.668636	1
GTTTTTT	35260	0.0	39.110184	2
CGTTTCT	1265	0.0	38.774704	1
TTGGGAC	4875	0.0	37.892307	5
TTACGGG	740	0.0	37.702705	3
TGGGACG	400	0.0	37.687504	6
CGTTTTC	1215	0.0	37.59259	1
CGTTTAT	485	0.0	37.1134	1
GCGATCG	195	0.0	36.92308	9
TGGGCGA	4490	0.0	36.88196	6
TTTGGGC	9615	0.0	36.856476	4
AGGGCGA	505	0.0	36.53465	6
TCGTTTG	1030	0.0	36.480583	1
TCTAGCG	105	0.0	36.42857	1
TTGGGCG	3995	0.0	36.214016	5
ACACGAC	305	0.0	36.147537	26
TCAAGCG	330	0.0	36.136364	17
CGTTTGG	1860	0.0	36.048386	2
TTCCGCA	25	0.0021076426	36.000004	1
>>END_MODULE