FastQCFastQC Report
Sat 14 Jan 2017
SRR2933213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431628
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT98680.40581865318214794No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC85150.3501769185089167No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC78680.3235692301618504No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77990.3207316250676502No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC61150.25147761088455967No Hit
TCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG31850.1309822061598238No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC29630.12185252020457077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG1050.040.71428724
ACGGGAC2350.040.2127655
TACGCGG1250.039.62
TACGGGA3500.039.2142874
ACGGGTA750.039.05
CGTTTTT46600.038.626611
TATTTCG700.038.5714261
TATCTCG356.2511663E-638.5714261
ACATACG4100.037.86585217
CGTTTGG15600.037.644232
TTTGGGA135650.037.635464
CGTTCAT4550.037.58241717
TTTGGGC63100.037.404914
TATGGGA14100.036.702134
TGGGCGA39750.036.6226436
TTGGGAC51750.036.521745
TCGTTCA4700.036.3829816
TAGCGGG5600.036.1607133
TTGGGAT62300.036.0072255
TCGTTTG8250.036.01
Produced by FastQC (version 0.11.5)