##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933213.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2431628
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06989062471727	33.0	31.0	34.0	30.0	34.0
2	32.25191600030926	34.0	31.0	34.0	30.0	34.0
3	32.32827389715861	34.0	31.0	34.0	30.0	34.0
4	35.84558534446881	37.0	35.0	37.0	35.0	37.0
5	35.03404097995253	37.0	35.0	37.0	33.0	37.0
6	34.751757670169944	37.0	35.0	37.0	32.0	37.0
7	35.57121936414616	37.0	35.0	37.0	33.0	37.0
8	35.84241380671715	37.0	35.0	37.0	35.0	37.0
9	37.67414793710222	39.0	37.0	39.0	35.0	39.0
10	37.11681556553881	39.0	37.0	39.0	33.0	39.0
11	37.11274051787527	39.0	37.0	39.0	33.0	39.0
12	37.01525151051065	39.0	37.0	39.0	33.0	39.0
13	36.98392023779953	39.0	37.0	39.0	33.0	39.0
14	38.09616067918284	40.0	37.0	41.0	33.0	41.0
15	38.15708118182551	40.0	37.0	41.0	33.0	41.0
16	38.13787511905604	40.0	37.0	41.0	33.0	41.0
17	38.06928814769364	40.0	37.0	41.0	33.0	41.0
18	38.035328594669906	40.0	37.0	41.0	33.0	41.0
19	38.03786845685278	40.0	37.0	41.0	33.0	41.0
20	37.948565323314256	40.0	37.0	41.0	33.0	41.0
21	37.83281900027471	40.0	36.0	41.0	33.0	41.0
22	37.87169377881814	40.0	37.0	41.0	33.0	41.0
23	37.7974793019327	40.0	36.0	41.0	33.0	41.0
24	37.736680117188975	39.0	36.0	41.0	33.0	41.0
25	37.619385860008194	39.0	36.0	41.0	33.0	41.0
26	37.58350167048578	39.0	36.0	41.0	33.0	41.0
27	37.54090346056223	39.0	36.0	41.0	33.0	41.0
28	37.46194483695697	39.0	36.0	41.0	32.0	41.0
29	37.401468481198606	39.0	36.0	41.0	32.0	41.0
30	37.30949183016481	39.0	36.0	41.0	32.0	41.0
31	37.25539144967898	39.0	36.0	41.0	32.0	41.0
32	37.262795131492155	39.0	36.0	41.0	32.0	41.0
33	37.14968695869599	39.0	35.0	41.0	32.0	41.0
34	37.039485480509356	39.0	35.0	41.0	31.0	41.0
35	36.93775158042266	39.0	35.0	41.0	31.0	41.0
36	36.78477094358183	39.0	35.0	41.0	31.0	41.0
37	36.76332317278794	39.0	35.0	41.0	31.0	41.0
38	36.6971613256633	39.0	35.0	41.0	31.0	41.0
39	36.66210374284224	39.0	35.0	41.0	31.0	41.0
40	36.565629282110585	39.0	35.0	41.0	30.0	41.0
41	36.44696433829517	39.0	35.0	40.0	30.0	41.0
42	36.34917141931249	39.0	35.0	40.0	30.0	41.0
43	36.1611521170179	38.0	35.0	40.0	30.0	41.0
44	36.10418040917443	38.0	35.0	40.0	30.0	41.0
45	36.14386164331057	38.0	35.0	40.0	30.0	41.0
46	35.91494710539605	38.0	35.0	40.0	29.0	41.0
47	35.84243478032002	38.0	35.0	40.0	29.0	41.0
48	35.77120390125463	38.0	35.0	40.0	29.0	41.0
49	35.8528615396763	38.0	35.0	40.0	29.0	41.0
50	35.649861327472784	38.0	34.0	40.0	28.0	41.0
51	34.5483972054936	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	25.0
10	33.0
11	49.0
12	22.0
13	26.0
14	39.0
15	58.0
16	108.0
17	206.0
18	448.0
19	865.0
20	1509.0
21	2542.0
22	4024.0
23	6192.0
24	9009.0
25	13357.0
26	18592.0
27	23367.0
28	28197.0
29	34290.0
30	43170.0
31	55207.0
32	70835.0
33	97248.0
34	152160.0
35	191450.0
36	221352.0
37	324550.0
38	523557.0
39	608922.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.547216926273265	4.3498018611399445	54.94828978774714	16.154691424839655
2	20.96957264844787	4.567474959163161	52.822306701518485	21.64064569087048
3	20.917796636656593	4.654988345256758	51.62681133791847	22.800403680168184
4	18.927483973699925	4.958488716201656	51.09979815991591	25.01422915018251
5	16.969988830528354	8.105351640958238	49.74399867084933	25.180660857664083
6	21.440327221104543	7.685057089324518	53.241079638826335	17.633536050744606
7	81.84594847567145	2.4216286372751092	10.615192784422616	5.11723010263083
8	83.33676039262585	2.9226509976032515	8.483945735120669	5.256642874650234
9	76.82881592085631	5.804588530811457	11.125221456571483	6.241374091760746
10	34.63839863663357	29.18287665712025	19.002577696917456	17.17614700932873
11	27.50535854990977	24.907181526121594	26.63088268435797	20.956577239610663
12	24.061657457472936	21.357008555584983	33.45211520841181	21.129218778530266
13	23.019104895979154	22.20314949490629	34.49257040961858	20.285175199495974
14	20.732200813611293	23.984014002141773	33.270302858825445	22.01348232542149
15	19.260265139240047	23.694413783687306	34.86520142061204	22.180119656460608
16	20.608292057831214	23.09218350833269	32.049844795338764	24.249679638497337
17	20.3197199571645	23.343907867486312	30.989197360780512	25.347174814568675
18	21.554119297853124	22.375009664307203	32.2384427223243	23.832428315515365
19	21.762415961652028	25.016491009315573	30.597237735377288	22.623855293655115
20	24.625066005161976	24.78129878418903	30.36048277121336	20.233152439435635
21	24.27571158088326	25.67748849741819	28.838045951107656	21.208753970590895
22	21.82323940997554	24.519827868407504	28.376667812675294	25.280264908941664
23	21.585250704466308	25.189009174100647	28.285699950814845	24.940040170618204
24	21.9393755952802	23.572766887040288	29.711000202333583	24.776857315345932
25	20.67335135144027	25.27849654634673	27.948189443451056	26.09996265876195
26	19.82149407721905	25.575745961142083	28.751848555782384	25.850911405856486
27	20.371084721840678	24.989143076161323	29.831084359943215	24.808687842054788
28	18.515948985617868	24.51004018706809	31.854543540377062	25.11946728693698
29	19.660943203483427	23.129524746383904	30.662626026678424	26.546906023454248
30	21.137155847851727	24.080780448325154	30.40518533262489	24.376878371198227
31	20.6345707484862	26.514129628380655	28.536272818046182	24.315026805086962
32	20.342338548495082	25.574018723258657	28.036730947332405	26.046911780913856
33	21.240584497299754	24.426968269817586	28.054291199147237	26.27815603373542
34	19.349546887928582	23.00680860723762	30.65074098505199	26.99290351978181
35	19.6032863579462	23.4014002141775	29.036267060586567	27.959046367289737
36	20.03414996043803	25.84885517028098	28.377860429309088	25.739134439971906
37	19.49558073850112	26.15276678834098	31.384858210219658	22.966794262938244
38	18.963755969251874	24.96927984050192	31.38703781992969	24.67992637031651
39	20.96056633662715	22.794934093537332	30.741009726816763	25.50348984301875
40	20.671459614710802	22.32981360635755	30.825150886566533	26.173575892365115
41	19.117603515011343	23.507913216988783	29.2665654450434	28.107917822956473
42	21.692545076796286	23.36451134795289	27.971795027857883	26.971148547392943
43	21.71294293370532	22.870027816754863	29.99019586877598	25.426833380763835
44	20.24824520855986	22.629695002689555	29.81200249380251	27.310057294948077
45	20.860756661792017	21.830353985066793	29.050496210769083	28.258393142372107
46	20.589662563517116	22.87718351655763	30.41320465136937	26.11994926855588
47	18.06370053314076	23.49898915459108	32.18440485139997	26.25290546086819
48	18.476099140164532	22.513887815076977	32.33463342254654	26.67537962221195
49	20.480969950995796	21.37859902912781	32.617448063601834	25.52298295627456
50	19.947788066266714	20.373469955108263	31.775707468412108	27.90303451021291
51	18.81763986925632	20.24590110000378	29.981436305224317	30.95502272551558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	471.0
1	1672.0
2	2873.0
3	8494.5
4	14116.0
5	9452.0
6	4788.0
7	4719.0
8	4650.0
9	4901.5
10	5153.0
11	5162.5
12	5172.0
13	5383.5
14	5595.0
15	5531.0
16	5467.0
17	5127.5
18	4788.0
19	5202.5
20	5617.0
21	6349.0
22	7081.0
23	8634.0
24	10187.0
25	11688.5
26	16089.0
27	18988.0
28	22765.5
29	26543.0
30	30810.5
31	35078.0
32	41131.0
33	47184.0
34	54156.0
35	61128.0
36	67012.0
37	72896.0
38	79578.5
39	86261.0
40	98012.5
41	109764.0
42	124258.0
43	138752.0
44	157954.0
45	177156.0
46	205298.0
47	233440.0
48	259189.0
49	284938.0
50	270583.0
51	256228.0
52	219647.5
53	183067.0
54	158525.5
55	133984.0
56	122807.5
57	111631.0
58	101705.5
59	91780.0
60	85259.0
61	78738.0
62	70893.5
63	63049.0
64	53443.0
65	43837.0
66	37226.0
67	30615.0
68	25768.5
69	20922.0
70	17385.5
71	13849.0
72	11921.0
73	9993.0
74	7910.5
75	4572.0
76	3316.0
77	2610.0
78	1904.0
79	1387.5
80	871.0
81	655.0
82	439.0
83	320.0
84	201.0
85	136.5
86	72.0
87	45.0
88	18.0
89	10.5
90	3.0
91	4.0
92	5.0
93	2.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2431628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.352241914625633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.34215032362219	20.646998130939203
2	11.965421301523188	7.024238813054466
3	4.954344687159218	4.362633713578129
4	2.60237488249535	3.0554214841419585
5	1.5168484425742705	2.226145121713156
6	0.9782967216458293	1.7229121222820134
7	0.68489525355934	1.407224781805683
8	0.5054123373880726	1.1867988154920877
9	0.42503911690280227	1.1228265884258908
>10	5.3732947132849045	38.44784799763431
>50	0.5584169780137029	10.221314267733954
>100	0.0847447440178481	4.562353309697405
>500	0.005313744575187611	1.0529682512012963
>1k	0.002728679646717962	1.2671955882686325
>5k	7.18073591241569E-4	1.6931210140317894
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	9868	0.40581865318214794	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	8515	0.3501769185089167	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	7868	0.3235692301618504	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7799	0.3207316250676502	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	6115	0.25147761088455967	No Hit
TCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	3185	0.1309822061598238	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	2963	0.12185252020457077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.467482690608925E-4	0.0	0.0	0.44254302056071076	0.0
2	2.467482690608925E-4	0.0	0.0	1.49562350820109	0.0
3	2.467482690608925E-4	0.0	0.0	2.184791423688163	0.0
4	2.467482690608925E-4	0.0	0.0	3.0610356518349024	0.0
5	2.467482690608925E-4	0.0	0.0	4.378219036793457	0.0
6	2.467482690608925E-4	0.0	0.0	5.417604995500956	0.0
7	2.467482690608925E-4	0.0	0.0	6.3461187319770955	0.0
8	2.467482690608925E-4	0.0	0.0	7.81287269269806	0.0
9	2.467482690608925E-4	0.0	0.0	8.657821015385577	0.0
10	2.467482690608925E-4	0.0	0.0	10.07687031075477	0.0
11	2.878729805710413E-4	0.0	0.0	12.414193289434074	0.0
12	2.878729805710413E-4	0.0	0.0	14.511718075297702	0.0
13	3.701224035913388E-4	0.0	0.0	15.185587598103	0.0
14	3.701224035913388E-4	0.0	0.0	15.4206153243835	0.0
15	3.701224035913388E-4	0.0	0.0	15.767625640106134	0.0
16	3.701224035913388E-4	0.0	0.0	16.59287522598029	0.0
17	3.701224035913388E-4	0.0	0.0	17.755100697968604	0.0
18	3.701224035913388E-4	0.0	0.0	19.024702791709917	0.0
19	3.701224035913388E-4	0.0	0.0	19.893421197650298	0.0
20	3.701224035913388E-4	0.0	0.0	20.80523830125332	0.0
21	3.701224035913388E-4	0.0	0.0	22.00377689350509	0.0
22	3.701224035913388E-4	0.0	0.0	23.262768811676786	0.0
23	3.701224035913388E-4	0.0	0.0	24.449627985859678	0.0
24	4.112471151014876E-4	0.0	0.0	25.362473207250453	0.0
25	4.112471151014876E-4	0.0	0.0	26.17476850899891	0.0
26	4.112471151014876E-4	0.0	0.0	26.870804251308176	0.0
27	4.523718266116363E-4	0.0	0.0	27.53542071402369	0.0
28	4.523718266116363E-4	0.0	0.0	28.249715828243463	0.0
29	4.523718266116363E-4	0.0	0.0	28.995882593883604	0.0
30	4.523718266116363E-4	0.0	0.0	29.83573145234386	0.0
31	4.523718266116363E-4	0.0	0.0	30.57346765212442	0.0
32	4.523718266116363E-4	0.0	0.0	31.31761930690056	0.0
33	4.93496538121785E-4	0.0	0.0	32.06711717417302	0.0
34	4.93496538121785E-4	0.0	0.0	32.781700161373365	0.0
35	4.93496538121785E-4	0.0	0.0	33.55817583939648	0.0
36	4.93496538121785E-4	0.0	0.0	34.26436938544876	0.0
37	4.93496538121785E-4	0.0	0.0	34.94555910690286	0.0
38	4.93496538121785E-4	0.0	0.0	35.648051428919224	0.0
39	4.93496538121785E-4	0.0	0.0	36.31826085240012	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	105	0.0	40.714287	24
ACGGGAC	235	0.0	40.212765	5
TACGCGG	125	0.0	39.6	2
TACGGGA	350	0.0	39.214287	4
ACGGGTA	75	0.0	39.0	5
CGTTTTT	4660	0.0	38.62661	1
TATTTCG	70	0.0	38.571426	1
TATCTCG	35	6.2511663E-6	38.571426	1
ACATACG	410	0.0	37.865852	17
CGTTTGG	1560	0.0	37.64423	2
TTTGGGA	13565	0.0	37.63546	4
CGTTCAT	455	0.0	37.582417	17
TTTGGGC	6310	0.0	37.40491	4
TATGGGA	1410	0.0	36.70213	4
TGGGCGA	3975	0.0	36.622643	6
TTGGGAC	5175	0.0	36.52174	5
TCGTTCA	470	0.0	36.38298	16
TAGCGGG	560	0.0	36.160713	3
TTGGGAT	6230	0.0	36.007225	5
TCGTTTG	825	0.0	36.0	1
>>END_MODULE