FastQCFastQC Report
Sat 14 Jan 2017
SRR2933212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450367
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260901.0647384657073817No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT34290.13993822149906524Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC27830.11357482368967588No Hit
TCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC25170.1027193069446332No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC24830.10133175969150743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT142800.043.046221
CTTACGG2500.038.6999972
CGTTATT3200.038.6718751
ACATACG2700.037.517
AGGCACG5900.037.37288310
TTTGGGA108400.037.2370834
ACGACCA22050.037.04081728
TACGGGA5600.036.9642834
CATACGA2750.036.81818418
ATACGAA2750.036.81818419
CACGACC22300.036.7264627
AACACGT19750.036.68354441
TTTGGGC62900.036.558034
TTGGGAC40000.036.4499975
CGACCAA22350.036.4429529
GGAACAT24500.036.3673448
TGGGCGA44300.036.2133186
ACACGAC22750.036.09890426
AGACACG22650.036.05960524
TTACGGG7200.035.93753
Produced by FastQC (version 0.11.5)