##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933212.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2450367
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24611374541038	33.0	31.0	34.0	30.0	34.0
2	32.45452905625974	34.0	31.0	34.0	31.0	34.0
3	32.48298765042134	34.0	31.0	34.0	30.0	34.0
4	35.98548421522164	37.0	35.0	37.0	35.0	37.0
5	35.2032226193056	37.0	35.0	37.0	35.0	37.0
6	34.919310454311535	37.0	35.0	37.0	32.0	37.0
7	35.69746899137966	37.0	35.0	37.0	35.0	37.0
8	35.977751904102526	37.0	35.0	37.0	35.0	37.0
9	37.85688225478061	39.0	38.0	39.0	35.0	39.0
10	37.38949879752706	39.0	37.0	39.0	35.0	39.0
11	37.305488116678035	39.0	37.0	39.0	34.0	39.0
12	37.05439552524173	39.0	37.0	39.0	33.0	39.0
13	36.970167734057796	39.0	37.0	39.0	33.0	39.0
14	37.95888819919628	40.0	37.0	41.0	33.0	41.0
15	38.071202803498416	40.0	37.0	41.0	33.0	41.0
16	38.11040754303335	40.0	37.0	41.0	33.0	41.0
17	38.054638346011025	40.0	37.0	41.0	33.0	41.0
18	37.96872509301668	40.0	37.0	41.0	33.0	41.0
19	37.909611499012186	40.0	37.0	41.0	33.0	41.0
20	37.75706332969715	40.0	35.0	41.0	33.0	41.0
21	37.62320134086037	39.0	35.0	41.0	33.0	41.0
22	37.70713162558915	39.0	35.0	41.0	33.0	41.0
23	37.64877016381628	39.0	35.0	41.0	33.0	41.0
24	37.58924969198492	39.0	35.0	41.0	33.0	41.0
25	37.481149150310955	39.0	35.0	41.0	33.0	41.0
26	37.42913204430194	39.0	35.0	41.0	33.0	41.0
27	37.39282891093457	39.0	35.0	41.0	33.0	41.0
28	37.284923442080306	39.0	35.0	41.0	33.0	41.0
29	37.18150546428351	39.0	35.0	41.0	33.0	41.0
30	37.03447932493378	39.0	35.0	41.0	32.0	41.0
31	36.86071719052697	39.0	35.0	41.0	31.0	41.0
32	36.71377634452308	39.0	35.0	41.0	31.0	41.0
33	36.46303145610433	39.0	35.0	41.0	31.0	41.0
34	36.170747483948325	39.0	35.0	41.0	30.0	41.0
35	35.94329992201168	39.0	35.0	41.0	29.0	41.0
36	35.80538098986805	38.0	35.0	40.0	29.0	41.0
37	35.79939658018574	38.0	35.0	40.0	29.0	41.0
38	35.69166414663599	38.0	35.0	40.0	29.0	41.0
39	35.638272960744246	38.0	35.0	40.0	28.0	41.0
40	35.53601358490381	38.0	35.0	40.0	27.0	41.0
41	35.39163806890968	38.0	35.0	40.0	27.0	41.0
42	35.28866737105095	38.0	34.0	40.0	26.0	41.0
43	35.16054207390158	38.0	34.0	40.0	26.0	41.0
44	35.08471996235666	38.0	34.0	40.0	26.0	41.0
45	35.05252927418628	37.0	34.0	40.0	26.0	41.0
46	34.88608685964184	37.0	34.0	40.0	25.0	41.0
47	34.78850270184017	37.0	34.0	40.0	25.0	41.0
48	34.70849754342921	37.0	34.0	40.0	25.0	41.0
49	34.74893475140663	37.0	34.0	40.0	26.0	41.0
50	34.5746718756823	37.0	34.0	40.0	24.0	41.0
51	33.582333585132346	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	21.0
10	63.0
11	53.0
12	37.0
13	31.0
14	58.0
15	108.0
16	161.0
17	347.0
18	677.0
19	1202.0
20	2245.0
21	3616.0
22	5737.0
23	8732.0
24	14124.0
25	23317.0
26	33055.0
27	37616.0
28	38919.0
29	40803.0
30	46931.0
31	57563.0
32	73970.0
33	105991.0
34	177844.0
35	193918.0
36	213332.0
37	307087.0
38	483521.0
39	579058.0
40	224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.545983111917522	4.248832929924375	53.02891362804021	19.176270330117898
2	24.515715400999117	4.09150955754791	48.97474541568671	22.418029625766263
3	20.349237481569087	4.158846409537836	52.77646164839798	22.715454460495103
4	18.11569450616989	4.629918701974031	52.40161983898738	24.852766952868695
5	17.48660506773067	7.608941844221702	51.19735125391421	23.707101834133418
6	21.849420923477993	7.065431423129678	53.28177370981572	17.803373943576613
7	81.27264201648161	1.8414384457511874	12.779718303421486	4.106201234345712
8	83.64138922863391	2.1080107592046415	10.694316402400132	3.5562836097613135
9	78.15319093017496	4.250710199737427	12.858890117276308	4.737208752811314
10	38.958490707718475	26.167182303712057	19.69288682062728	15.18144016794219
11	32.45595455701126	22.267276697735483	26.68286015931491	18.59390858593835
12	26.706938185177975	21.2221679446385	32.33013666932341	19.740757200860116
13	25.134561475893204	20.632337931420068	34.988391534819065	19.24470905786766
14	21.540079506457605	21.37300249309593	36.97650188726831	20.110416113178147
15	20.578386829401474	20.04667055996102	37.85294202868387	21.522000581953645
16	24.839544443750672	20.904501244099354	33.64883709256613	20.60711721958384
17	23.87858635053443	21.017872016722393	33.0864315427036	22.017110090039573
18	25.806052725979416	19.50030342393609	33.30468456357762	21.38895928650688
19	24.386306214538475	23.321200456911146	30.638675757549787	21.653817571000587
20	26.07189045559298	25.396277373960718	29.372375648219222	19.159456522227078
21	25.593757996251178	24.874518796572108	30.41393391275674	19.11778929441998
22	23.937149006659002	21.54762939592314	31.73034896405314	22.784872633364717
23	22.479122515117123	23.80941303894478	31.610162885804456	22.101301560133646
24	22.276989528507364	23.27994133123732	32.08327568890701	22.35979345134831
25	24.13699662132244	24.2141279245109	28.83433379571305	22.81454165845361
26	22.373872974946202	23.39110835234069	29.871158075504606	24.3638605972085
27	21.901494755683537	21.953731828742388	31.934767322609225	24.21000609296485
28	20.199096706738214	24.739600231312288	30.45294847669757	24.608354585251924
29	22.526095070656762	24.8135075276479	29.32385230457315	23.336545097122187
30	24.96560719271848	22.99006638597402	30.72870308814965	21.31562333315785
31	25.227486331639305	24.64108437634036	28.514912256000834	21.616517036019502
32	25.54180659468561	24.29966613164477	27.809466908426373	22.349060365243247
33	25.289395425256707	24.64447162404652	26.869934177206922	23.196198773489847
34	23.336953199255458	25.171984441514272	29.6397233557259	21.85133900350437
35	22.69472287212487	27.886271729908213	28.050900130470254	21.368105267496666
36	24.637778749060853	29.185383250753866	25.439291338807617	20.737546661377664
37	24.516654035905642	27.21441318790206	25.723452854205103	22.545479921987198
38	24.66777425585637	26.46526010185413	27.46241685429162	21.40454878799788
39	24.627045662955794	23.8217377233696	27.01309640555884	24.538120208115764
40	26.299284964252294	22.911261864039144	28.13015356475173	22.659299606956836
41	22.201449823638665	24.037215649737366	28.01992517855489	25.741409348069084
42	22.826417430531833	24.425769690825906	29.312303014201547	23.435509864440714
43	22.421253632619116	22.985822123787987	30.300114227787102	24.292810015805795
44	23.91184667439612	23.59593481303005	28.414519131215854	24.077699381357974
45	23.693756894375415	23.150940246909954	27.28787157189107	25.867431286823567
46	22.83751780855684	23.642295215369778	28.184063856556996	25.336123119516387
47	20.913479491031346	25.90477263201798	29.99220116823317	23.189546708717508
48	22.05722652974024	25.31282048770653	29.28487855084565	23.345074431707577
49	23.706204009440217	23.71648818319868	29.111639195271565	23.465668612089534
50	22.096404334534377	24.089493533009545	28.832660576966635	24.981441555489443
51	21.32264268985013	22.80311479872199	28.564986387753343	27.309256123674537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	403.0
1	1620.5
2	2838.0
3	23102.0
4	43366.0
5	28121.0
6	12876.0
7	12383.0
8	11890.0
9	12004.5
10	12119.0
11	11914.5
12	11710.0
13	11497.5
14	11285.0
15	10863.0
16	10441.0
17	10139.0
18	9837.0
19	9620.0
20	9403.0
21	9182.5
22	8962.0
23	10107.0
24	11252.0
25	12095.5
26	15646.5
27	18354.0
28	20800.0
29	23246.0
30	26558.5
31	29871.0
32	33950.5
33	38030.0
34	45397.0
35	52764.0
36	56807.5
37	60851.0
38	66560.5
39	72270.0
40	78377.5
41	84485.0
42	93889.0
43	103293.0
44	116051.5
45	128810.0
46	151254.5
47	173699.0
48	200274.5
49	226850.0
50	228159.0
51	229468.0
52	215785.0
53	202102.0
54	186969.5
55	171837.0
56	159541.5
57	147246.0
58	137794.5
59	128343.0
60	117170.5
61	105998.0
62	93987.0
63	81976.0
64	74070.0
65	66164.0
66	57044.0
67	47924.0
68	40038.0
69	32152.0
70	26408.5
71	20665.0
72	17007.0
73	13349.0
74	11765.5
75	8045.5
76	5909.0
77	4442.0
78	2975.0
79	2180.0
80	1385.0
81	963.5
82	542.0
83	367.5
84	193.0
85	129.5
86	66.0
87	47.0
88	28.0
89	21.0
90	14.0
91	8.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2450367.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.305695060597913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05155291493412	21.701322284887897
2	9.383112706190284	5.499572793736681
3	3.4246606218306836	3.0108617960822293
4	1.8762442742027572	2.1993857023591543
5	1.1827363345180926	1.7330455178238273
6	0.8878868359272069	1.5612084517201112
7	0.6590754894981249	1.3520265722002445
8	0.5720373582328905	1.3411161906914477
9	0.53904115550149	1.4217278155415105
>10	7.035404423388461	46.58957642869522
>50	0.2957883534569473	5.462421166211455
>100	0.08505257284349754	4.64816576577503
>500	0.004130804322828052	0.8028523497775328
>1k	0.0031337136242143844	1.5876297727389859
>5k	0.0	0.0
>10k+	1.424415283733811E-4	1.0890873917587394
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26090	1.0647384657073817	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	3429	0.13993822149906524	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	2783	0.11357482368967588	No Hit
TCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	2517	0.1027193069446332	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	2483	0.10133175969150743	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.26481706617825E-4	0.0	0.0	0.14761054160458412	0.0
2	3.26481706617825E-4	0.0	0.0	0.4930689974195702	0.0
3	3.26481706617825E-4	0.0	0.0	0.8284473305427309	0.0
4	3.26481706617825E-4	0.0	0.0	1.2291220049894567	0.0
5	3.26481706617825E-4	0.0	0.0	1.9990882998342698	0.0
6	3.26481706617825E-4	0.0	0.0	3.027056763333819	0.0
7	3.26481706617825E-4	0.0	0.0	3.885581221098717	0.0
8	3.26481706617825E-4	0.0	0.0	5.103521227636513	0.0
9	3.26481706617825E-4	0.0	0.0	6.037422149416802	0.0
10	3.26481706617825E-4	0.0	0.0	7.214592752840697	0.0
11	3.26481706617825E-4	0.0	0.0	8.709838158937009	0.0
12	3.26481706617825E-4	0.0	0.0	9.84717799415353	0.0
13	3.26481706617825E-4	0.0	0.0	10.283439174621597	0.0
14	3.26481706617825E-4	0.0	0.0	10.541033241143062	0.0
15	3.26481706617825E-4	0.0	0.0	10.766101567642725	0.0
16	3.26481706617825E-4	0.0	0.0	11.143596040919585	0.0
17	3.26481706617825E-4	0.0	0.0	11.660702253988893	0.0
18	3.26481706617825E-4	0.0	0.0	12.23008635033038	0.0
19	3.26481706617825E-4	0.0	0.0	12.620884953151915	0.0
20	3.26481706617825E-4	0.0	0.0	13.080652816496467	0.0
21	3.26481706617825E-4	0.0	0.0	13.660729188729688	0.0
22	3.26481706617825E-4	0.0	0.0	14.294756662981504	0.0
23	3.26481706617825E-4	0.0	0.0	14.894585178465103	0.0
24	3.26481706617825E-4	0.0	0.0	15.382226417512152	0.0
25	3.26481706617825E-4	0.0	0.0	15.828037187898792	0.0
26	3.26481706617825E-4	0.0	0.0	16.210632937841556	0.0
27	3.26481706617825E-4	0.0	0.0	16.62726440569923	0.0
28	3.6729191994505314E-4	0.0	0.0	17.041039158623995	0.0
29	3.6729191994505314E-4	0.0	0.0	17.517865691139328	0.0
30	3.6729191994505314E-4	0.0	0.0	18.148914019818257	0.0
31	3.6729191994505314E-4	0.0	0.0	18.58125741980691	0.0
32	3.6729191994505314E-4	0.0	0.0	19.06183849194835	0.0
33	3.6729191994505314E-4	0.0	0.0	19.526544391105496	0.0
34	3.6729191994505314E-4	0.0	0.0	20.011981878632874	0.0
35	3.6729191994505314E-4	0.0	0.0	20.617074911635687	0.0
36	3.6729191994505314E-4	0.0	0.0	21.11361277718807	0.0
37	3.6729191994505314E-4	0.0	0.0	21.590888222050005	0.0
38	4.0810213327228126E-4	0.0	0.0	22.189165949427167	0.0
39	4.489123465995094E-4	0.0	0.0	23.126780600620233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14280	0.0	43.04622	1
CTTACGG	250	0.0	38.699997	2
CGTTATT	320	0.0	38.671875	1
ACATACG	270	0.0	37.5	17
AGGCACG	590	0.0	37.372883	10
TTTGGGA	10840	0.0	37.237083	4
ACGACCA	2205	0.0	37.040817	28
TACGGGA	560	0.0	36.964283	4
CATACGA	275	0.0	36.818184	18
ATACGAA	275	0.0	36.818184	19
CACGACC	2230	0.0	36.72646	27
AACACGT	1975	0.0	36.683544	41
TTTGGGC	6290	0.0	36.55803	4
TTGGGAC	4000	0.0	36.449997	5
CGACCAA	2235	0.0	36.44295	29
GGAACAT	2450	0.0	36.367344	8
TGGGCGA	4430	0.0	36.213318	6
ACACGAC	2275	0.0	36.098904	26
AGACACG	2265	0.0	36.059605	24
TTACGGG	720	0.0	35.9375	3
>>END_MODULE