##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933211.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1724444
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15924843021867	33.0	31.0	34.0	30.0	34.0
2	32.33798024174749	34.0	31.0	34.0	30.0	34.0
3	32.42063064964707	34.0	31.0	34.0	30.0	34.0
4	35.91719475958628	37.0	35.0	37.0	35.0	37.0
5	35.11270995172937	37.0	35.0	37.0	35.0	37.0
6	34.7672287415538	37.0	35.0	37.0	32.0	37.0
7	35.60734590395513	37.0	35.0	37.0	33.0	37.0
8	35.87086968321383	37.0	35.0	37.0	35.0	37.0
9	37.74793788606647	39.0	38.0	39.0	35.0	39.0
10	37.21478575123344	39.0	37.0	39.0	34.0	39.0
11	37.17273915534514	39.0	37.0	39.0	34.0	39.0
12	37.0451223698769	39.0	37.0	39.0	33.0	39.0
13	36.99308762708444	39.0	37.0	39.0	33.0	39.0
14	38.140956157462924	40.0	37.0	41.0	33.0	41.0
15	38.21486983630666	40.0	37.0	41.0	33.0	41.0
16	38.21967950249472	40.0	37.0	41.0	34.0	41.0
17	38.16487633115369	40.0	37.0	41.0	33.0	41.0
18	38.11356414009385	40.0	37.0	41.0	33.0	41.0
19	38.123713498379765	40.0	37.0	41.0	34.0	41.0
20	38.02538035447947	40.0	37.0	41.0	33.0	41.0
21	37.93039495628736	40.0	37.0	41.0	33.0	41.0
22	37.96153078905433	40.0	37.0	41.0	33.0	41.0
23	37.86976381952676	40.0	36.0	41.0	33.0	41.0
24	37.807060710582654	40.0	36.0	41.0	33.0	41.0
25	37.69695507653481	40.0	36.0	41.0	33.0	41.0
26	37.67089856208726	40.0	36.0	41.0	33.0	41.0
27	37.601885013372424	40.0	36.0	41.0	33.0	41.0
28	37.51408685930074	39.0	36.0	41.0	33.0	41.0
29	37.405421689541676	39.0	36.0	41.0	32.0	41.0
30	37.3041838412845	39.0	35.0	41.0	32.0	41.0
31	37.239576350406274	39.0	35.0	41.0	32.0	41.0
32	37.17813219797222	39.0	35.0	41.0	32.0	41.0
33	37.08464641356866	39.0	35.0	41.0	31.0	41.0
34	36.9887181027624	39.0	35.0	41.0	31.0	41.0
35	36.81120349515554	39.0	35.0	41.0	31.0	41.0
36	36.706442192382006	39.0	35.0	41.0	31.0	41.0
37	36.62886762341949	39.0	35.0	41.0	30.0	41.0
38	36.51897307190028	39.0	35.0	41.0	30.0	41.0
39	36.47700708170286	39.0	35.0	41.0	30.0	41.0
40	36.422388897522914	39.0	35.0	40.0	30.0	41.0
41	36.33839544804006	39.0	35.0	40.0	30.0	41.0
42	36.262848779084734	39.0	35.0	40.0	30.0	41.0
43	36.126915689926726	38.0	35.0	40.0	30.0	41.0
44	36.04613892941725	38.0	35.0	40.0	30.0	41.0
45	36.040854327539776	38.0	35.0	40.0	29.0	41.0
46	35.80468487234146	38.0	35.0	40.0	28.0	41.0
47	35.711470479760436	38.0	34.0	40.0	28.0	41.0
48	35.61278301875851	38.0	34.0	40.0	28.0	41.0
49	35.67179450303982	38.0	34.0	40.0	28.0	41.0
50	35.47327602404021	38.0	34.0	40.0	28.0	41.0
51	34.39432999853866	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	16.0
10	40.0
11	39.0
12	33.0
13	38.0
14	36.0
15	56.0
16	82.0
17	182.0
18	374.0
19	625.0
20	1214.0
21	2003.0
22	3152.0
23	4648.0
24	6491.0
25	9420.0
26	12992.0
27	16368.0
28	19945.0
29	23987.0
30	29941.0
31	38573.0
32	49770.0
33	69157.0
34	110270.0
35	139008.0
36	156254.0
37	231456.0
38	365368.0
39	432730.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.50091507755543	4.654659704809203	53.35783591696802	16.486589300667344
2	20.195784844274446	5.853480890072394	52.29459466355533	21.656139602097834
3	21.302518376937726	5.9595440617381605	50.71802853557437	22.019909025749747
4	18.594225153150813	4.583912263894914	51.49671430327688	25.32514827967739
5	18.274179967572156	8.184435099081211	49.28510290853168	24.256282024814954
6	21.592408915569308	8.751110502863533	52.65210119899515	17.004379382572004
7	82.7711424667893	2.2042467021254386	10.562476949091998	4.462133881993269
8	84.6034431967637	3.5558707618223613	7.440833103307501	4.399852938106427
9	78.78759762566949	5.143222975057467	9.39926144310862	6.669917956164421
10	36.67988058759809	27.82966567774889	18.061357747772615	17.42909598688041
11	29.95835179338964	24.70071512905029	25.000753866173675	20.340179211386396
12	25.94453632591142	21.650224652119757	31.243229701863324	21.162009320105497
13	23.82153320142608	22.361062464191356	33.20519541371016	20.612208920672405
14	21.030720626474388	24.65890455126406	31.702102242809854	22.608272579451697
15	19.17331035394597	23.99782190665513	35.09078868319296	21.73807905620594
16	20.886384249068104	22.32847224960625	32.868333213487944	23.916810287837702
17	21.223652377229993	22.292634611503768	30.73257235375576	25.75114065751048
18	21.86026336604726	22.309219667324655	31.642778773912056	24.18773819271603
19	23.395366854475995	24.472815585777212	29.570806590414065	22.561010969332724
20	25.93259044654393	24.073266513728484	29.252733054828106	20.74140998489948
21	25.691179301850337	24.652815632168977	28.290219920159775	21.365785145820915
22	23.53506405542888	23.291449301919922	27.961070350791328	25.212416291859867
23	22.803581908139666	25.381340304469152	27.694027756192718	24.12105003119846
24	22.845856403571236	23.41369160146691	29.073370895198686	24.66708109976317
25	22.384084377341335	25.18788664636254	27.641373103446675	24.786655872849455
26	22.147138439984136	25.32265472233369	28.006360310917604	24.52384652676457
27	22.31119131731735	25.230335110911113	28.630271554193698	23.82820201757784
28	21.57008287888734	24.832293771209734	29.753532152972205	23.84409119693072
29	22.68592079534041	24.030992018296914	28.99862216459334	24.284465021769336
30	23.22348536687767	24.947693285487958	29.325278176618085	22.503543171016283
31	23.644142691789355	25.97144354934112	26.25536114828896	24.12905261058057
32	23.478350123286116	26.53411766343239	27.098415489282342	22.889116723999155
33	22.807873146359057	24.99112757503288	27.241186144635606	24.959813133972457
34	22.83222882273939	24.288118373226386	27.93236544648594	24.947287357548287
35	22.47738981376026	24.58114035596401	27.72476230019647	25.21670753007926
36	23.869316718896062	26.050715476988522	26.729542971531693	23.35042483258372
37	22.422415572787518	26.8485378475613	28.006824228562945	22.722222351088234
38	21.825295573529786	25.818582685201726	28.229504698325954	24.126617042942534
39	21.528736218746445	23.365502156057257	29.94623194490514	25.159529680291154
40	21.92526982610047	23.73860792232163	30.131972972157982	24.204149279419916
41	20.367898290695436	25.240715268225582	27.760367979476285	26.631018461602697
42	21.73587544739058	25.51854394807834	27.118421937737615	25.62715866679347
43	22.052035322689516	24.34280266567079	27.950806172888186	25.654355838751503
44	22.51925838125216	23.55275091565745	28.80418268149038	25.12380802160001
45	22.77545690089095	22.992628348615554	27.975857725736525	26.256057024756963
46	22.747041945113903	24.467596512267143	28.537777973654116	24.247583568964835
47	21.007466754501742	24.825972893291983	30.084421413510675	24.082138938695604
48	21.151861121613692	24.120934051787128	29.187668605069227	25.53953622152995
49	21.646629290368374	23.111971162879165	30.457295220952375	24.784104325800083
50	21.25148743595037	22.413659127231732	29.92152833029081	26.41332510652709
51	20.303007810053558	22.90900719304309	28.269459605530827	28.518525391372524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	1124.0
2	1918.0
3	5362.5
4	8807.0
5	6097.5
6	3388.0
7	3317.5
8	3247.0
9	3363.5
10	3480.0
11	3660.0
12	3840.0
13	3830.0
14	3820.0
15	3815.0
16	3810.0
17	4056.0
18	4302.0
19	4297.0
20	4292.0
21	4838.5
22	5385.0
23	6128.5
24	6872.0
25	7976.0
26	11790.0
27	14500.0
28	16898.5
29	19297.0
30	21477.5
31	23658.0
32	27701.5
33	31745.0
34	37475.0
35	43205.0
36	46344.5
37	49484.0
38	54870.0
39	60256.0
40	67403.0
41	74550.0
42	81720.0
43	88890.0
44	100694.0
45	112498.0
46	137178.5
47	161859.0
48	169227.0
49	176595.0
50	170763.0
51	164931.0
52	146159.0
53	127387.0
54	114591.5
55	101796.0
56	93728.5
57	85661.0
58	83378.5
59	81096.0
60	74636.5
61	68177.0
62	61378.0
63	54579.0
64	47842.5
65	41106.0
66	34833.0
67	28560.0
68	23885.0
69	19210.0
70	16308.5
71	13407.0
72	10775.5
73	8144.0
74	6713.0
75	4085.0
76	2888.0
77	2177.5
78	1467.0
79	1149.0
80	831.0
81	650.5
82	470.0
83	348.0
84	226.0
85	144.5
86	63.0
87	47.5
88	32.0
89	22.0
90	12.0
91	8.5
92	5.0
93	3.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1724444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.757304464350373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16583769030683	24.188216973133667
2	9.916734625148056	6.298575215659845
3	3.6035191611726525	3.4331416543344115
4	1.7286212068241928	2.195853998745947
5	1.0223078109619204	1.623287020450062
6	0.6553442708822557	1.2487180523625299
7	0.49760651109694715	1.1061849033444224
8	0.3795716635769722	0.96433383090031
9	0.32207604161410125	0.9205440222790613
>10	5.162016309288943	41.838842854406415
>50	0.4724940367649062	9.144911710235277
>100	0.06771479404483384	3.830079028183875
>500	0.0036211048926629063	0.7988808978951095
>1k	0.0025347734248640347	2.4084298380691274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	4842	0.2807861548417925	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	4792	0.27788666955842	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4761	0.276088988682729	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	4534	0.26292532549621794	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	4387	0.25440083876310277	No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	4047	0.23468433883616982	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	3998	0.23184284325846477	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	3559	0.20638536247045425	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3195882266979965E-4	0.0	0.0	0.253530993178091	0.0
2	2.3195882266979965E-4	0.0	0.0	1.0547167666795791	0.0
3	2.3195882266979965E-4	0.0	0.0	1.5362052928364156	0.0
4	2.3195882266979965E-4	0.0	0.0	2.151708028790729	0.0
5	2.3195882266979965E-4	0.0	0.0	3.3469918420082068	0.0
6	2.3195882266979965E-4	0.0	0.0	4.0879263113212145	0.0
7	2.3195882266979965E-4	0.0	0.0	4.792559224886398	0.0
8	2.3195882266979965E-4	0.0	0.0	5.876734762045042	0.0
9	2.3195882266979965E-4	0.0	0.0	6.446715579050407	0.0
10	2.3195882266979965E-4	0.0	0.0	7.727882146361378	0.0
11	2.3195882266979965E-4	0.0	0.0	9.295053942024213	0.0
12	2.3195882266979965E-4	0.0	0.0	10.996703865129863	0.0
13	2.899485283372496E-4	0.0	0.0	11.462709139873489	0.0
14	2.899485283372496E-4	0.0	0.0	11.637026195109843	0.0
15	2.899485283372496E-4	0.0	0.0	11.939964417516602	0.0
16	2.899485283372496E-4	0.0	0.0	12.54398519174876	0.0
17	2.899485283372496E-4	0.0	0.0	13.395332060652592	0.0
18	2.899485283372496E-4	0.0	0.0	14.304262707284202	0.0
19	2.899485283372496E-4	0.0	0.0	14.971608240105217	0.0
20	2.899485283372496E-4	0.0	0.0	15.607465362748805	0.0
21	2.899485283372496E-4	0.0	0.0	16.47876069040224	0.0
22	2.899485283372496E-4	0.0	0.0	17.42022356191329	0.0
23	2.899485283372496E-4	0.0	0.0	18.33205369382827	0.0
24	2.899485283372496E-4	0.0	0.0	19.038368308857812	0.0
25	3.4793823400469946E-4	0.0	0.0	19.684489609404537	0.0
26	3.4793823400469946E-4	0.0	0.0	20.229882791206904	0.0
27	4.059279396721494E-4	0.0	0.0	20.773072364193908	0.0
28	4.059279396721494E-4	0.0	0.0	21.349200090000025	0.0
29	4.059279396721494E-4	0.0	0.0	21.95490256569654	0.0
30	4.059279396721494E-4	0.0	0.0	22.637615370519427	0.0
31	4.639176453395993E-4	0.0	0.0	23.255089756466433	0.0
32	5.219073510070492E-4	0.0	0.0	23.864677542442667	0.0
33	5.219073510070492E-4	0.0	0.0	24.467306563738806	0.0
34	5.219073510070492E-4	0.0	0.0	25.077242287949044	0.0
35	5.219073510070492E-4	0.0	0.0	25.715129050290994	0.0
36	5.219073510070492E-4	0.0	0.0	26.33080575536231	0.0
37	5.219073510070492E-4	0.0	0.0	26.92131492817395	0.0
38	5.798970566744992E-4	0.0	0.0	27.512925905393274	0.0
39	5.798970566744992E-4	0.0	0.0	28.110567812002014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	35	1.2123928E-7	45.0	1
CTCGATT	20	7.034271E-4	45.0	16
CTTTACG	30	2.1661363E-6	44.999996	1
ACACGCG	290	0.0	41.12069	36
ATGCGAA	150	0.0	40.5	26
TAATGCG	150	0.0	40.5	24
TTTGGGC	4845	0.0	39.380806	4
CGAGATA	335	0.0	38.955223	19
GCTACGA	185	0.0	38.91892	10
CGAATAT	175	0.0	38.57143	14
TTACGGG	375	0.0	38.4	3
TTGGGAC	3420	0.0	38.223686	5
TACGAAA	255	0.0	37.941174	20
GGACGTA	30	1.1401591E-4	37.499996	8
TTTGGGA	9240	0.0	37.183437	4
TATGGGA	970	0.0	36.881443	4
TTGGGAT	4250	0.0	36.158825	5
CGTTTTT	3045	0.0	36.133003	1
CGTTTGG	1130	0.0	36.039825	2
GTCGACG	25	0.0021074	36.000004	37
>>END_MODULE