FastQCFastQC Report
Sat 14 Jan 2017
SRR2933209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195268
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307401.4002846121749144No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC294861.3431617460829384No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC198680.9050375626119452No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG196350.8944238243348877No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT80010.3644657508787082No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC66400.3024687646337486TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC53440.24343269250041455No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT51220.2333200319960934No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT48560.22120306040082577No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT46250.21068042717335653No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC42440.19332491522675135No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT41590.18945295061924103TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATAACCCT37890.1725985164453725No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC35130.16002601960216248TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTA31050.14144058948611288No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC29590.13478992086615393TruSeq Adapter, Index 13 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT29340.13365110774629796No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC28740.13091795625864358No Hit
CGTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT27080.12335623714279988No Hit
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG26780.12198966139897269No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGT22780.10376865148127701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA302.166409E-644.99999622
CGTTTTT263450.044.171571
CGTTATT5900.043.4745751
CGATGAA14000.042.58928719
CGTATGG1500.042.02
CCGATGA13850.041.75090418
TACGGGT1050.040.7142874
TAATACG1400.040.178574
GTTTTTT296400.039.3752
GCGAGAC2800.038.57142621
CGTTTTC7050.037.6595731
TTACGGG4850.037.5773163
GATGAAT16450.037.47720320
CTTACGG1150.037.1739122
TCTTGCG4550.036.5934071
CGAGACA3100.036.2903222
ATAGCGT250.002107525236.012
ACTCGAC250.002107525236.035
CGTTCTG6550.035.725191
TCGTTTG7000.035.678571
Produced by FastQC (version 0.11.5)