##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933209.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2195268
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.992030130262	33.0	31.0	34.0	30.0	34.0
2	32.19817534806684	34.0	31.0	34.0	30.0	34.0
3	32.18252167844655	33.0	31.0	34.0	30.0	34.0
4	35.84966482452256	37.0	35.0	37.0	35.0	37.0
5	35.0446200646117	37.0	35.0	37.0	33.0	37.0
6	34.680481836386264	37.0	35.0	37.0	32.0	37.0
7	35.55995987733616	37.0	35.0	37.0	33.0	37.0
8	35.72779861046578	37.0	35.0	37.0	35.0	37.0
9	37.66326343753929	39.0	37.0	39.0	35.0	39.0
10	37.1625081766782	39.0	37.0	39.0	34.0	39.0
11	37.107083964235805	39.0	37.0	39.0	33.0	39.0
12	37.091065874417154	39.0	37.0	39.0	33.0	39.0
13	37.074990843942516	39.0	37.0	39.0	33.0	39.0
14	38.259494968268115	40.0	38.0	41.0	33.0	41.0
15	38.28057029938942	40.0	38.0	41.0	33.0	41.0
16	38.31065546438977	40.0	38.0	41.0	33.0	41.0
17	38.183846345867565	40.0	37.0	41.0	33.0	41.0
18	37.98035501815723	39.0	37.0	41.0	33.0	41.0
19	37.796790642418145	39.0	37.0	41.0	33.0	41.0
20	37.44234781356992	39.0	35.0	41.0	33.0	41.0
21	37.38923356965983	39.0	35.0	41.0	33.0	41.0
22	37.45888474664597	39.0	35.0	41.0	33.0	41.0
23	37.338554563725246	39.0	35.0	41.0	33.0	41.0
24	37.24798748945459	39.0	35.0	41.0	33.0	41.0
25	37.17083062295811	39.0	35.0	41.0	33.0	41.0
26	37.1327427904019	39.0	35.0	41.0	32.0	41.0
27	37.01375230723538	39.0	35.0	41.0	32.0	41.0
28	36.88355544744423	39.0	35.0	41.0	32.0	41.0
29	36.78401498131436	39.0	35.0	40.0	31.0	41.0
30	36.58959498339155	39.0	35.0	40.0	31.0	41.0
31	36.37994859853102	39.0	35.0	40.0	30.0	41.0
32	35.94032254831756	38.0	35.0	40.0	29.0	41.0
33	35.392977987197916	38.0	35.0	40.0	24.0	41.0
34	34.92482239070583	38.0	35.0	40.0	21.0	41.0
35	34.587547852927294	38.0	34.0	40.0	18.0	41.0
36	34.42684446728144	38.0	34.0	40.0	18.0	41.0
37	34.359830781480895	38.0	34.0	40.0	16.0	41.0
38	34.27873453264021	38.0	34.0	40.0	16.0	41.0
39	34.20136539137818	38.0	34.0	40.0	15.0	41.0
40	34.09784454563179	38.0	33.0	40.0	15.0	41.0
41	33.97438262663147	38.0	33.0	40.0	15.0	41.0
42	33.925620470940224	38.0	33.0	40.0	15.0	41.0
43	33.823047573234795	38.0	33.0	40.0	15.0	41.0
44	33.76672870920544	38.0	33.0	40.0	14.0	41.0
45	33.821639544693404	38.0	33.0	40.0	15.0	41.0
46	33.59372841949138	38.0	33.0	40.0	13.0	41.0
47	33.4766215332251	38.0	33.0	40.0	13.0	41.0
48	33.39066300788787	38.0	33.0	40.0	12.0	41.0
49	33.48705533902922	38.0	33.0	40.0	12.0	41.0
50	33.3042070489799	38.0	33.0	40.0	12.0	41.0
51	32.386727269745656	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	20.0
10	20.0
11	24.0
12	26.0
13	21.0
14	36.0
15	66.0
16	132.0
17	319.0
18	836.0
19	1707.0
20	3414.0
21	5926.0
22	9720.0
23	15501.0
24	26127.0
25	45055.0
26	62278.0
27	59112.0
28	48774.0
29	43380.0
30	44931.0
31	52356.0
32	64417.0
33	83692.0
34	119810.0
35	149977.0
36	178436.0
37	257816.0
38	419415.0
39	501752.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.768061120555668	3.8152517141415077	46.80471814830809	23.611969016994735
2	28.315130544425553	9.8083240861708	42.706084177421616	19.170461191982028
3	15.831779992237848	9.855334291758455	54.58959908311878	19.723286632884914
4	14.006626981307067	3.7282463917845106	60.68680452682771	21.57832210008072
5	17.66645348085063	6.7577626057501865	54.72876204636519	20.847021867034
6	16.699874457241666	10.278198379423378	57.97661151166965	15.045315651665309
7	69.67636753234684	1.4114905332742973	24.567296566979522	4.3448453673993335
8	70.98531933230932	5.315296355615806	19.221070047028427	4.478314265046454
9	65.92666590138425	3.795026393132866	20.449211668005912	9.829096037476972
10	32.3641122632863	18.486262269572553	31.640738169553785	17.508887297587357
11	24.64883558636121	22.10345160590871	33.859510547231594	19.388202260498492
12	18.037979873072445	19.63213603077164	40.661641312131366	21.668242784024546
13	19.262887264789537	20.46816151832031	43.791919710941904	16.477031505948247
14	18.22383417423294	24.298809985842276	39.83759613860357	17.639759701321207
15	15.895280211801019	22.751254061007586	43.46494368796885	17.888522039222547
16	16.36765989391728	22.831289847071066	39.14656433747497	21.654485921536686
17	17.165193498014823	23.905600591818402	38.96430868577322	19.96489722439356
18	18.10562537239189	22.420861598674968	38.501267271239776	20.97224575769337
19	18.407957479451255	25.330802435055766	35.69172419950548	20.569515885987496
20	19.681833835322156	24.480382349672112	37.784634951176805	18.053148863828927
21	19.346066175063818	26.369764420562774	36.6531102352879	17.631059169085507
22	18.30396106534601	21.847901941813028	37.53277504158945	22.31536195125151
23	18.121386545970697	24.670700798262445	36.65634446454829	20.551568191218568
24	18.61604141271134	23.373865969895245	36.49285645306177	21.517236164331642
25	17.82001104193201	27.432368166437993	34.415296902246105	20.332323889383893
26	17.593250573506285	23.68785952330194	36.595850711621544	22.123039191570232
27	20.02789636618399	23.927602461294022	34.85865962606843	21.185841546453553
28	17.438371989205873	23.48082329811212	37.05597676456816	22.024827948113852
29	18.582514754462782	22.536565011652335	34.86225827552718	24.018661958357704
30	17.82529513480814	26.148424702587565	35.29669270448984	20.729587458114455
31	17.843242829577072	24.467217670006576	34.6422851332958	23.04725436712055
32	17.951247865864215	27.352833458147252	32.95214980585514	21.743768870133394
33	17.511893764223778	26.83403575326566	31.12681458482518	24.527255897685386
34	17.276068343364	25.624752877552993	30.787994905405625	26.311183873677386
35	16.734448823560495	26.907284213134798	28.98429713365293	27.373969829651777
36	17.264133581867906	26.427479469477078	29.071712428733075	27.236674519921937
37	16.56740771514002	28.397535061778335	30.76544640563248	24.26961081744917
38	17.485245537219146	27.139738747159804	30.40257499312157	24.972440722499485
39	19.248811534628118	26.59310844962893	29.03955234622834	25.118527669514613
40	18.972717681850234	25.570636478097438	30.128029926186688	25.328615913865644
41	17.62522844591184	25.015396753380454	29.371356936829578	27.988017863878124
42	18.779165003999513	24.64327817833631	31.345147836163967	25.23240898150021
43	19.711989606735944	24.30746496555318	30.48169972868916	25.498845699021714
44	19.890373293830184	24.805035193880656	30.61065892638165	24.693932585907508
45	19.51984905715384	23.94295366214968	29.540174593717033	26.99702268697945
46	21.75629581445181	25.436985370351138	28.72373669182988	24.08298212336717
47	17.887109910953924	24.668605382121907	33.19330487211584	24.250979834808323
48	18.744681742730272	24.98934070919815	30.273889110577844	25.992088437493738
49	18.884619098898174	23.107155937224977	33.126615975817074	24.88160898805977
50	19.242251971057748	22.58343855966561	31.98989827210163	26.184411197175017
51	18.07770167469302	22.530233210705937	30.504430438561485	28.887634676039553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	259.0
1	818.0
2	1377.0
3	26000.0
4	50623.0
5	35201.0
6	19779.0
7	19905.5
8	20032.0
9	20534.0
10	21036.0
11	21543.0
12	22050.0
13	21980.0
14	21910.0
15	21542.5
16	21175.0
17	20613.0
18	20051.0
19	19446.0
20	18841.0
21	18544.0
22	18247.0
23	18382.5
24	18518.0
25	18669.0
26	20606.5
27	22393.0
28	24557.5
29	26722.0
30	30061.0
31	33400.0
32	37943.0
33	42486.0
34	49138.5
35	55791.0
36	61486.5
37	67182.0
38	74847.5
39	82513.0
40	90439.0
41	98365.0
42	110039.0
43	121713.0
44	139027.5
45	156342.0
46	224887.0
47	293432.0
48	260656.0
49	227880.0
50	210669.0
51	193458.0
52	166200.0
53	138942.0
54	119577.5
55	100213.0
56	88085.0
57	75957.0
58	67176.0
59	58395.0
60	50097.5
61	41800.0
62	35832.0
63	29864.0
64	25205.5
65	20547.0
66	16891.5
67	13236.0
68	10931.5
69	8627.0
70	6824.0
71	5021.0
72	3977.5
73	2934.0
74	2624.0
75	1875.0
76	1436.0
77	1171.0
78	906.0
79	637.5
80	369.0
81	276.5
82	184.0
83	131.0
84	78.0
85	54.0
86	30.0
87	21.0
88	12.0
89	7.5
90	3.0
91	3.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2195268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.34345456727469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9199801245597	31.435611780381212
2	8.471201389304097	6.835150567592481
3	3.0848088379456438	3.733555356071625
4	1.8401979317856156	2.969597666231433
5	1.2396296004294356	2.50054702325869
6	0.9646495182834931	2.335037640852811
7	0.7972409942580139	2.251441908171196
8	0.6721738092615439	2.1694250828204047
9	0.5938250156069255	2.156125728424426
>10	4.284616224776094	29.101978739767826
>50	0.0850415248367159	2.34993087906794
>100	0.04023556048165077	3.016473002184495
>500	0.0031997342354706864	0.8715306580073761
>1k	0.0022855244539076325	2.518282857190907
>5k	4.5710489078152646E-4	1.1575074180380445
>10k+	4.5710489078152646E-4	4.597803691939145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30740	1.4002846121749144	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	29486	1.3431617460829384	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC	19868	0.9050375626119452	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG	19635	0.8944238243348877	No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT	8001	0.3644657508787082	No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	6640	0.3024687646337486	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC	5344	0.24343269250041455	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT	5122	0.2333200319960934	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT	4856	0.22120306040082577	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT	4625	0.21068042717335653	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	4244	0.19332491522675135	No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT	4159	0.18945295061924103	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATAACCCT	3789	0.1725985164453725	No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	3513	0.16002601960216248	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTA	3105	0.14144058948611288	No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	2959	0.13478992086615393	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT	2934	0.13365110774629796	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC	2874	0.13091795625864358	No Hit
CGTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT	2708	0.12335623714279988	No Hit
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG	2678	0.12198966139897269	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGT	2278	0.10376865148127701	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.82210099176957E-4	0.0	0.0	0.20507746662366508	0.0
2	1.82210099176957E-4	0.0	0.0	0.8720575346609161	0.0
3	1.82210099176957E-4	0.0	0.0	1.319656643289111	0.0
4	1.82210099176957E-4	0.0	0.0	2.293569623389946	0.0
5	1.82210099176957E-4	0.0	0.0	4.808570069804689	0.0
6	1.82210099176957E-4	0.0	0.0	6.5314576625724055	0.0
7	1.82210099176957E-4	0.0	0.0	8.012598006257095	0.0
8	1.82210099176957E-4	0.0	0.0	9.699180236763803	0.0
9	2.2776262397119622E-4	0.0	0.0	10.675234185529968	0.0
10	2.2776262397119622E-4	0.0	0.0	12.79124006727197	0.0
11	2.2776262397119622E-4	0.0	0.0	14.231428691166636	0.0
12	2.2776262397119622E-4	0.0	0.0	16.468285421187755	0.0
13	2.2776262397119622E-4	0.0	0.0	17.04074400027696	0.0
14	2.7331514876543546E-4	0.0	0.0	17.313649176319245	0.0
15	2.7331514876543546E-4	0.0	0.0	17.69738364518592	0.0
16	2.7331514876543546E-4	0.0	0.0	18.183474637265245	0.0
17	2.7331514876543546E-4	0.0	0.0	18.79888924723542	0.0
18	2.7331514876543546E-4	0.0	0.0	19.483589247417626	0.0
19	2.7331514876543546E-4	0.0	0.0	20.208147706794797	0.0
20	2.7331514876543546E-4	0.0	0.0	20.71236860374223	0.0
21	2.7331514876543546E-4	0.0	0.0	21.33183738841909	0.0
22	2.7331514876543546E-4	0.0	0.0	22.01471528760953	0.0
23	2.7331514876543546E-4	0.0	0.0	22.678096706188036	0.0
24	2.7331514876543546E-4	0.0	0.0	23.2184407552973	0.0
25	2.7331514876543546E-4	0.0	0.0	23.678931228442266	0.0
26	2.7331514876543546E-4	0.0	0.0	24.104983992842786	0.0
27	2.7331514876543546E-4	0.0	0.0	24.54989550250812	0.0
28	2.7331514876543546E-4	0.0	0.0	25.00214096866533	0.0
29	2.7331514876543546E-4	0.0	0.0	25.496568072782	0.0
30	2.7331514876543546E-4	0.0	0.0	26.038916432982216	0.0
31	3.188676735596747E-4	0.0	0.0	26.53070149066082	0.0
32	3.188676735596747E-4	0.0	0.0	27.01661027264097	0.0
33	3.188676735596747E-4	0.0	0.0	27.482111523513304	0.0
34	3.188676735596747E-4	0.0	0.0	27.966790387324007	0.0
35	3.188676735596747E-4	0.0	0.0	28.45433906019675	0.0
36	3.188676735596747E-4	0.0	0.0	28.933004990734617	0.0
37	3.188676735596747E-4	0.0	0.0	29.417638302020528	0.0
38	3.188676735596747E-4	0.0	0.0	29.90518697489327	0.0
39	3.188676735596747E-4	0.0	0.0	30.448400833064575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	30	2.166409E-6	44.999996	22
CGTTTTT	26345	0.0	44.17157	1
CGTTATT	590	0.0	43.474575	1
CGATGAA	1400	0.0	42.589287	19
CGTATGG	150	0.0	42.0	2
CCGATGA	1385	0.0	41.750904	18
TACGGGT	105	0.0	40.714287	4
TAATACG	140	0.0	40.17857	4
GTTTTTT	29640	0.0	39.375	2
GCGAGAC	280	0.0	38.571426	21
CGTTTTC	705	0.0	37.659573	1
TTACGGG	485	0.0	37.577316	3
GATGAAT	1645	0.0	37.477203	20
CTTACGG	115	0.0	37.173912	2
TCTTGCG	455	0.0	36.593407	1
CGAGACA	310	0.0	36.29032	22
ATAGCGT	25	0.0021075252	36.0	12
ACTCGAC	25	0.0021075252	36.0	35
CGTTCTG	655	0.0	35.72519	1
TCGTTTG	700	0.0	35.67857	1
>>END_MODULE