FastQCFastQC Report
Sat 14 Jan 2017
SRR2933208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985874
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGC452524.590038889350971No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCG419544.255513382034621No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTC418134.241211351552024No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGCAT137741.3971359423212297No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCT108361.0991262575136376No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCT97650.9904916855500804No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC96430.9781168790332233No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTC95010.9637134157103241No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGCATCGTA76780.7788013478395819No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.34497308986746783No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGCT32870.3334097460730276No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTT32760.3322939848297044No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTTCGCATCGT30650.3108916555259597No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC29680.30105267001665525No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGC27570.27965034071291056No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATG25320.2568279516449364No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCG22900.2322812042918263No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTAT19780.2006341581175688No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC18250.1851149335513463No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTTCGCA14600.14809194684107704No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCCATCGAATGC10360.10508442255298343No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA207.0327986E-445.032
CTCGAAG207.0327986E-445.014
TTATTCG207.0327986E-445.01
GCCGACG650.044.99999643
CGATCGC1200.044.99999634
CGATGAA40800.043.45588319
CCGATGA40250.043.3229818
ATGAATG41500.043.0481921
CGACGAA2100.042.85714319
GATGAAT42750.042.5789520
TGCTACG6700.042.3134354
GCGAACC1600.042.187533
CTACGGC6750.042.06
ACCGATG41350.041.95284317
AATACGG2900.041.896555
TGAATGA42400.041.86910222
ACGGCTG57000.041.7236828
AATGCTA9600.041.7187462
ACCACCG56400.041.52925514
CCACCGA53800.041.44516815
Produced by FastQC (version 0.11.5)