##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	985874
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.693531830639614	31.0	31.0	34.0	30.0	34.0
2	31.817803289264145	31.0	31.0	34.0	30.0	34.0
3	31.957150710942777	33.0	31.0	34.0	30.0	34.0
4	35.6814755232413	37.0	35.0	37.0	35.0	37.0
5	34.906297356457316	37.0	35.0	37.0	33.0	37.0
6	33.87250906302428	36.0	35.0	37.0	28.0	37.0
7	35.12454126997974	37.0	35.0	37.0	32.0	37.0
8	34.938324775782704	37.0	35.0	37.0	32.0	37.0
9	37.09039086130682	39.0	37.0	39.0	34.0	39.0
10	36.76409662898099	38.0	35.0	39.0	32.0	39.0
11	36.72469199918042	39.0	35.0	39.0	32.0	39.0
12	36.625921770936245	39.0	35.0	39.0	32.0	39.0
13	36.400548143068995	38.0	35.0	39.0	32.0	39.0
14	37.465933780584535	39.0	36.0	41.0	32.0	41.0
15	37.3580437256688	39.0	36.0	41.0	32.0	41.0
16	37.689153989252176	39.0	36.0	41.0	33.0	41.0
17	37.203127377332194	39.0	36.0	41.0	31.0	41.0
18	37.280366456565446	39.0	36.0	40.0	32.0	41.0
19	37.32893250050209	39.0	36.0	40.0	32.0	41.0
20	36.64963372601367	39.0	35.0	40.0	30.0	41.0
21	36.87305578603351	39.0	35.0	40.0	31.0	41.0
22	36.88549246658295	39.0	35.0	40.0	31.0	41.0
23	36.658114525791326	39.0	35.0	40.0	30.0	41.0
24	36.715417994591604	39.0	35.0	40.0	30.0	41.0
25	36.86557917137484	39.0	35.0	40.0	31.0	41.0
26	36.759882094466434	39.0	35.0	40.0	31.0	41.0
27	36.449528032994074	39.0	35.0	40.0	30.0	41.0
28	36.30945130919367	39.0	35.0	40.0	30.0	41.0
29	36.43005698496968	39.0	35.0	40.0	30.0	41.0
30	36.292457251129456	38.0	35.0	40.0	30.0	41.0
31	36.33959410634625	39.0	35.0	40.0	30.0	41.0
32	35.88334614768216	38.0	35.0	40.0	28.0	41.0
33	35.75063040510248	38.0	35.0	40.0	27.0	41.0
34	35.50817142961474	38.0	35.0	40.0	26.0	41.0
35	35.377802842959646	38.0	35.0	40.0	25.0	41.0
36	35.222470619977805	38.0	35.0	40.0	25.0	41.0
37	35.19135203890152	38.0	35.0	40.0	24.0	41.0
38	35.1098913248549	38.0	34.0	40.0	24.0	41.0
39	35.052716675761815	38.0	34.0	40.0	24.0	41.0
40	35.130212380081026	38.0	34.0	40.0	24.0	41.0
41	34.70082688051414	38.0	33.0	40.0	23.0	41.0
42	34.87096626952329	38.0	34.0	40.0	24.0	41.0
43	34.666129748832	38.0	34.0	40.0	23.0	41.0
44	34.84865205898522	38.0	34.0	40.0	23.0	41.0
45	34.91488263206049	38.0	34.0	40.0	24.0	41.0
46	34.72071177452697	38.0	34.0	40.0	23.0	41.0
47	34.320112915037825	37.0	33.0	40.0	23.0	41.0
48	34.301828631244966	37.0	33.0	40.0	23.0	41.0
49	34.53362498656015	37.0	33.0	40.0	24.0	41.0
50	34.25822163887069	37.0	33.0	40.0	23.0	41.0
51	33.084409366714205	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	14.0
11	19.0
12	13.0
13	7.0
14	23.0
15	17.0
16	44.0
17	106.0
18	301.0
19	834.0
20	1971.0
21	4297.0
22	7784.0
23	10599.0
24	12345.0
25	13316.0
26	13717.0
27	13915.0
28	14327.0
29	16589.0
30	20905.0
31	27616.0
32	36545.0
33	48801.0
34	69804.0
35	86352.0
36	91776.0
37	125797.0
38	188015.0
39	179960.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.778594424845366	3.0896443156021967	38.32132706613624	11.810434193416198
2	15.056082217403036	32.699614758072535	35.92609197524227	16.318211049282162
3	13.964867721432961	32.708642280859415	37.45235192326808	15.874138074439534
4	13.710068426594068	3.1996989473299835	64.2555742417388	18.834658384337146
5	34.71021651854091	5.771832911710828	38.105579414813654	21.412371154934608
6	15.42834074131177	27.65789543085628	42.40491178385879	14.508852043973164
7	61.59062922848153	1.5985815631612152	32.201579512189184	4.60920969616807
8	59.11729085055494	22.33693149428832	11.997374918092982	6.548402737063763
9	56.355680340489755	3.3262871320270135	10.975439052049248	29.342593475433983
10	43.874470774155725	17.17592714687678	20.390536721731177	18.55906535723632
11	35.37977469737512	18.221598297551207	25.707443344687047	20.69118366038662
12	21.083627319515475	15.878296820891919	31.198104423080437	31.839971436512172
13	25.922988130329028	19.051623229743356	40.67477182682574	14.350616813101876
14	19.807602188514963	32.60913666452305	26.416661764079386	21.166599382882602
15	12.454735595015185	19.796241710401127	42.90254129838093	24.846481396202762
16	14.259834421031492	21.99297273282387	26.61668732515514	37.1305055209895
17	15.13519983283868	27.416180972416353	33.53318983967525	23.915429355069715
18	15.702615141488668	21.46633342597533	31.18643964644569	31.64461178609031
19	16.470766041096528	24.629820849317458	27.48769112482934	31.411721984756674
20	23.558994354248107	23.179534098677923	36.609445020357576	16.652026526716394
21	19.81034087520312	35.53628556996127	26.974643818581278	17.67872973625433
22	15.86196613360328	20.273584656862845	35.2822977378448	28.582151471689084
23	21.22999490807142	29.860002393815034	28.10470709238706	20.805295605726492
24	20.82862515899598	25.43458900427438	25.15067848426878	28.58610735246086
25	14.478219326201927	39.030444052688274	23.381081152358213	23.110255468751582
26	16.350365259657927	20.63042538904566	35.43840287906974	27.580806472226676
27	26.01082897003065	24.88735883084451	24.79434491628748	24.30746728283736
28	13.585914630064288	25.997946999312287	36.747495116008736	23.66864325461469
29	21.367233541000168	16.88664068633517	36.745770757723605	25.000355014941057
30	21.380521243079745	29.308613473932777	30.281861576631496	19.029003706355986
31	22.833850978928343	18.712837543134313	28.970334951525246	29.482976526412095
32	25.70287886687345	25.956460967628725	28.137875631165848	20.202784534331975
33	22.794900768252333	19.17222687686256	22.557243623424494	35.47562873146061
34	25.907976069964317	19.576842476827668	29.762728299965307	24.75245315324271
35	23.965030013977444	19.69298307897358	25.290351505364782	31.05163540168419
36	18.930309552742035	26.731103569015918	32.514601257361484	21.82398562088056
37	23.439405035531923	19.2415055067889	36.43224184834979	20.886847609329386
38	17.566646447720498	21.1040153204162	28.216181783879072	33.113156447984224
39	24.79931512546228	22.813665843708222	31.8270894657938	20.559929565035695
40	18.337738899697122	18.63808153983166	27.16472896130743	35.859450599163786
41	23.75445543751027	27.23137033738591	22.517786248546976	26.49638797655684
42	18.36644439350262	19.705662184011345	33.05138384824024	28.876509574245794
43	21.589472894102084	25.81668651369242	25.681679403250314	26.91216118895518
44	23.813489350566098	18.273836210306794	30.84065509385581	27.072019345271304
45	19.264632194377782	17.075305769297092	30.01671613208179	33.64334590424335
46	29.300600279548906	23.20154502502348	27.227515889454434	20.270338805973175
47	15.665795020459003	20.47553744190434	39.79017602655106	24.068491511085597
48	23.093519050101737	23.168985083286504	26.494055021229894	27.243440845381866
49	19.55483155048211	16.29751874986053	38.07819254793209	26.069457151725274
50	22.562010966918695	18.477919084994635	30.265226590821953	28.69484335726472
51	21.02611489906418	16.979857466572806	27.935009950561636	34.05901768380138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	357.0
1	622.0
2	887.0
3	3334.5
4	5782.0
5	3937.0
6	2092.0
7	1973.5
8	1855.0
9	1923.5
10	1992.0
11	2119.5
12	2247.0
13	2263.5
14	2280.0
15	2141.0
16	2002.0
17	2017.0
18	2032.0
19	2035.0
20	2038.0
21	2263.5
22	2489.0
23	2884.0
24	3279.0
25	3405.0
26	4468.0
27	5405.0
28	6176.0
29	6947.0
30	7870.5
31	8794.0
32	10557.0
33	12320.0
34	14300.0
35	16280.0
36	18505.5
37	20731.0
38	22824.5
39	24918.0
40	28844.5
41	32771.0
42	37238.5
43	41706.0
44	50492.5
45	59279.0
46	152955.0
47	246631.0
48	183331.0
49	120031.0
50	108772.0
51	97513.0
52	81037.5
53	64562.0
54	58145.0
55	51728.0
56	45215.5
57	38703.0
58	34385.0
59	30067.0
60	25804.0
61	21541.0
62	18976.5
63	16412.0
64	14397.5
65	12383.0
66	10670.0
67	8957.0
68	7383.0
69	5809.0
70	4602.0
71	3395.0
72	2886.5
73	2378.0
74	2040.0
75	1327.0
76	952.0
77	694.5
78	437.0
79	382.5
80	328.0
81	276.0
82	224.0
83	146.5
84	69.0
85	39.5
86	10.0
87	13.5
88	17.0
89	13.0
90	9.0
91	5.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	985874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.092935270855175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.54029762259131	23.79862519591222
2	10.327664834180156	6.422348283765018
3	3.737682664487691	3.486465754497399
4	1.781394225736024	2.2155510141074153
5	1.0675379609686668	1.6596444359789742
6	0.7535107113025125	1.4057315863455038
7	0.5446348705002859	1.185400774230119
8	0.4452255696010209	1.1074695853227337
9	0.3627099536900485	1.0149945400961602
>10	4.211833877623362	27.958148389886304
>50	0.15639145276942365	3.224669966257211
>100	0.05920991495529487	3.3388318191234063
>500	0.0051486882569814355	1.1609369372875558
>1k	0.0038615161927360764	2.9891399639287886
>5k	0.0012871720642453589	3.6607084137326384
>10k+	0.0016089650803066988	15.371333339528565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGC	45252	4.590038889350971	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCG	41954	4.255513382034621	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTC	41813	4.241211351552024	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGCAT	13774	1.3971359423212297	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCT	10836	1.0991262575136376	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCT	9765	0.9904916855500804	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC	9643	0.9781168790332233	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTC	9501	0.9637134157103241	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGCATCGTA	7678	0.7788013478395819	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3401	0.34497308986746783	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGCT	3287	0.3334097460730276	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTT	3276	0.3322939848297044	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTTCGCATCGT	3065	0.3108916555259597	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC	2968	0.30105267001665525	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGC	2757	0.27965034071291056	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATG	2532	0.2568279516449364	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCG	2290	0.2322812042918263	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTAT	1978	0.2006341581175688	No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGCATCGTATGCCGTCTTCTGC	1825	0.1851149335513463	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTTCGCA	1460	0.14809194684107704	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCCATCGAATGC	1036	0.10508442255298343	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0143284030210757E-4	0.0	0.0	0.3551163738976786	0.0
2	1.0143284030210757E-4	0.0	0.0	1.9345271302417957	0.0
3	1.0143284030210757E-4	0.0	0.0	2.433475271687863	0.0
4	1.0143284030210757E-4	0.0	0.0	4.102248360338137	0.0
5	1.0143284030210757E-4	0.0	0.0	9.423618028267304	0.0
6	1.0143284030210757E-4	0.0	0.0	10.321400097781257	0.0
7	1.0143284030210757E-4	0.0	0.0	11.972523872218964	0.0
8	1.0143284030210757E-4	0.0	0.0	13.873273866640158	0.0
9	1.0143284030210757E-4	0.0	0.0	14.30649352757046	0.0
10	1.0143284030210757E-4	0.0	0.0	19.9488981350558	0.0
11	1.0143284030210757E-4	0.0	0.0	21.6527669864506	0.0
12	1.0143284030210757E-4	0.0	0.0	28.452317436102383	0.0
13	1.0143284030210757E-4	0.0	0.0	29.276256397876402	0.0
14	1.0143284030210757E-4	0.0	0.0	29.678741908195164	0.0
15	1.0143284030210757E-4	0.0	0.0	30.845828168711215	0.0
16	1.0143284030210757E-4	0.0	0.0	31.766026895931933	0.0
17	2.0286568060421515E-4	0.0	0.0	32.67740096604637	0.0
18	3.0429852090632273E-4	0.0	0.0	33.565952647092836	0.0
19	3.0429852090632273E-4	0.0	0.0	35.981474306047225	0.0
20	3.0429852090632273E-4	0.0	0.0	36.779142162183	0.0
21	3.0429852090632273E-4	0.0	0.0	37.6554204695529	0.0
22	3.0429852090632273E-4	0.0	0.0	38.749779383572346	0.0
23	3.0429852090632273E-4	0.0	0.0	39.623724735615305	0.0
24	3.0429852090632273E-4	0.0	0.0	40.295717302616765	0.0
25	3.0429852090632273E-4	0.0	0.0	40.84913487930506	0.0
26	3.0429852090632273E-4	0.0	0.0	41.44231412939179	0.0
27	3.0429852090632273E-4	0.0	0.0	42.1166396517202	0.0
28	3.0429852090632273E-4	0.0	0.0	42.6557551979259	0.0
29	3.0429852090632273E-4	0.0	0.0	43.222460476693776	0.0
30	3.0429852090632273E-4	0.0	0.0	43.84688104159355	0.0
31	3.0429852090632273E-4	0.0	0.0	44.392995453780095	0.0
32	3.0429852090632273E-4	0.0	0.0	44.92206914879589	0.0
33	3.0429852090632273E-4	0.0	0.0	45.440492395579966	0.0
34	3.0429852090632273E-4	0.0	0.0	46.00963206251509	0.0
35	3.0429852090632273E-4	0.0	0.0	46.554833579138915	0.0
36	3.0429852090632273E-4	0.0	0.0	47.064533601657004	0.0
37	3.0429852090632273E-4	0.0	0.0	47.57474078837661	0.0
38	4.057313612084303E-4	0.0	0.0	48.07663048219143	0.0
39	5.071642015105379E-4	0.0	0.0	48.59231504228735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGATA	20	7.0327986E-4	45.0	32
CTCGAAG	20	7.0327986E-4	45.0	14
TTATTCG	20	7.0327986E-4	45.0	1
GCCGACG	65	0.0	44.999996	43
CGATCGC	120	0.0	44.999996	34
CGATGAA	4080	0.0	43.455883	19
CCGATGA	4025	0.0	43.32298	18
ATGAATG	4150	0.0	43.04819	21
CGACGAA	210	0.0	42.857143	19
GATGAAT	4275	0.0	42.57895	20
TGCTACG	670	0.0	42.313435	4
GCGAACC	160	0.0	42.1875	33
CTACGGC	675	0.0	42.0	6
ACCGATG	4135	0.0	41.952843	17
AATACGG	290	0.0	41.89655	5
TGAATGA	4240	0.0	41.869102	22
ACGGCTG	5700	0.0	41.723682	8
AATGCTA	960	0.0	41.718746	2
ACCACCG	5640	0.0	41.529255	14
CCACCGA	5380	0.0	41.445168	15
>>END_MODULE