##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933207.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174491
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09291023605802	33.0	31.0	34.0	30.0	34.0
2	32.28196296657134	34.0	31.0	34.0	30.0	34.0
3	32.358883839281106	34.0	31.0	34.0	30.0	34.0
4	35.86988440664562	37.0	35.0	37.0	35.0	37.0
5	35.11592001879753	37.0	35.0	37.0	35.0	37.0
6	34.82141772355021	37.0	35.0	37.0	32.0	37.0
7	35.56485434778871	37.0	35.0	37.0	33.0	37.0
8	35.811680831676135	37.0	35.0	37.0	35.0	37.0
9	37.62339031812529	39.0	37.0	39.0	35.0	39.0
10	37.20371251239319	39.0	37.0	39.0	34.0	39.0
11	37.155881965258956	39.0	37.0	39.0	34.0	39.0
12	37.091368609269246	39.0	37.0	39.0	33.0	39.0
13	37.05071894825521	39.0	37.0	39.0	33.0	39.0
14	38.1689485417586	40.0	37.0	41.0	33.0	41.0
15	38.22561622089391	40.0	37.0	41.0	33.0	41.0
16	38.19804459828874	40.0	37.0	41.0	33.0	41.0
17	38.150907496661716	40.0	37.0	41.0	33.0	41.0
18	38.1254219415328	40.0	37.0	41.0	33.0	41.0
19	38.140987214240276	40.0	37.0	41.0	34.0	41.0
20	38.067464797611336	40.0	37.0	41.0	34.0	41.0
21	37.96194646142208	40.0	37.0	41.0	33.0	41.0
22	37.98951235307265	40.0	37.0	41.0	33.0	41.0
23	37.88149532067556	40.0	37.0	41.0	33.0	41.0
24	37.820208492128536	40.0	36.0	41.0	33.0	41.0
25	37.69996733355875	40.0	36.0	41.0	33.0	41.0
26	37.58082651827315	40.0	36.0	41.0	33.0	41.0
27	37.53530554584477	39.0	36.0	41.0	33.0	41.0
28	37.469302141657735	39.0	36.0	41.0	32.0	41.0
29	37.367795473692055	39.0	36.0	41.0	32.0	41.0
30	37.23751368265412	39.0	36.0	41.0	32.0	41.0
31	37.207397516204274	39.0	36.0	41.0	32.0	41.0
32	37.19523069957763	39.0	35.0	41.0	32.0	41.0
33	37.09582729195202	39.0	35.0	41.0	31.0	41.0
34	37.016069596712725	39.0	35.0	41.0	31.0	41.0
35	36.90945664819389	39.0	35.0	41.0	31.0	41.0
36	36.773186009593616	39.0	35.0	41.0	31.0	41.0
37	36.78997197563198	39.0	35.0	41.0	31.0	41.0
38	36.71527471330899	39.0	35.0	41.0	31.0	41.0
39	36.62016379068261	39.0	35.0	41.0	30.0	41.0
40	36.52062284014648	39.0	35.0	40.0	30.0	41.0
41	36.44519201563404	39.0	35.0	40.0	30.0	41.0
42	36.38048953814237	39.0	35.0	40.0	30.0	41.0
43	36.235376036586416	39.0	35.0	40.0	30.0	41.0
44	36.16473055916924	38.0	35.0	40.0	30.0	41.0
45	36.11825251732181	38.0	35.0	40.0	30.0	41.0
46	35.813480351422136	38.0	35.0	40.0	28.0	41.0
47	35.73556802356568	38.0	35.0	40.0	28.0	41.0
48	35.682860434062505	38.0	34.0	40.0	28.0	41.0
49	35.76254362689193	38.0	35.0	40.0	28.0	41.0
50	35.576940931050885	38.0	34.0	40.0	28.0	41.0
51	34.42265217117215	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	7.0
12	6.0
13	5.0
14	4.0
15	0.0
16	12.0
17	15.0
18	59.0
19	78.0
20	159.0
21	275.0
22	391.0
23	548.0
24	655.0
25	883.0
26	1226.0
27	1588.0
28	1786.0
29	2318.0
30	3026.0
31	3799.0
32	5042.0
33	6945.0
34	10738.0
35	13660.0
36	15781.0
37	23758.0
38	38228.0
39	43472.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.436217340722443	4.442636009880166	54.46527328057035	16.655873368827045
2	20.734593761282817	4.500518651391762	51.95110349530921	22.813784092016206
3	21.456694041526497	4.551524147377228	50.98830312165097	23.003478689445302
4	19.25371509132276	4.856984027829515	49.58937710254397	26.299923778303754
5	16.74642245158776	7.676040598082423	50.18310399963322	25.3944329506966
6	21.221724902717046	7.377457863156265	53.051446779490064	18.34937045463663
7	79.8052621625184	2.0178691164587286	12.096898980463177	6.079969740559685
8	80.78238992268942	2.7199110555845287	9.398765552378059	7.09893346934799
9	73.41811325512491	6.448470121668167	12.754812569129639	7.378604054077289
10	33.315758405877666	29.07829057086039	19.984411803474107	17.62153921978784
11	25.56464230246832	26.217971127450703	27.813468889512926	20.40391768056805
12	23.319827383647294	21.367291149686803	34.894636399585075	20.41824506708082
13	23.39604907989524	23.146179459112503	33.56218945389733	19.895582007094923
14	20.12940495498335	25.405321764446303	32.89797181516525	21.56730146540509
15	18.094342974709296	25.326234590895808	34.50665077281923	22.07277166157567
16	19.30013582362414	24.660870761242702	30.92709652646841	25.11189688866475
17	19.470918270856377	24.193797960926354	30.10355834971431	26.23172541850296
18	21.04349221449817	23.13185207259973	31.658366334080267	24.16628937882183
19	22.092256907233036	25.49587084720702	29.34592615091896	23.065946094640985
20	23.97315620862967	25.768091190949676	29.610123158214464	20.648629442206186
21	24.201248201912993	26.35150236974973	28.747041394685112	20.700208033652164
22	21.536927405998014	25.305603154317414	28.241571198514535	24.91589824117003
23	21.461851900671096	25.65920305345261	27.689107174582073	25.189837871294223
24	22.39943607406686	24.754285321305968	27.96132751832473	24.884951086302447
25	20.85035904430601	26.24376042317369	26.62830747717647	26.27757305534383
26	19.777524342229686	28.308050271933794	27.28908654314549	24.62533884269103
27	20.11507756847058	27.5269211592575	27.71661575668659	24.64138551558533
28	19.206721263560873	27.49769329077144	27.66790264254317	25.627682803124518
29	21.459559518829053	27.352127043801687	26.640915577307712	24.54739786006155
30	21.923193746382335	26.802528497171775	27.771632920895634	23.502644835550257
31	22.549013989260192	27.52290949103392	26.011656761666792	23.916419758039094
32	23.723286587846935	26.8724461433541	25.426526296485207	23.977740972313757
33	22.52952874360282	25.558911347863212	27.056409786178083	24.85515012235588
34	21.0927784241021	24.636800751901244	29.316698282432906	24.953722541563746
35	21.675616507441646	26.193328022648732	26.512542194153284	25.618513275756342
36	21.8527030047395	27.62320119662332	27.149824346241353	23.374271452395824
37	21.68650532119135	26.934340453089273	27.769913634514097	23.60924059120528
38	23.299195947068903	25.832850977987405	26.952106412365108	23.915846662578584
39	23.613825354889364	23.400060748118815	29.576883621504834	23.40923027548699
40	23.421265280157716	23.63846845969133	30.513321603979577	22.42694465617138
41	20.753505911479674	25.82941240522434	27.690253365503093	25.726828317792894
42	22.608615917153323	25.850043841802727	25.418502960038055	26.122837281005896
43	22.390266546698683	24.534216664469803	26.264964955212587	26.810551833618927
44	21.677908889283685	23.739906356201754	27.24782366998871	27.33436108452585
45	23.487744353576975	22.934134138723486	27.380208721366717	26.19791278633282
46	22.585119003272375	24.107260546389213	30.45486586700747	22.852754583330945
47	20.29388335214997	24.645397183808907	30.738548119960342	24.322171344080783
48	20.170667828140132	23.41152265732903	30.36718226154931	26.050627252981528
49	21.91860898269825	21.715733189677405	31.723699216578506	24.64195861104584
50	22.011450447301005	21.34264804488484	29.180301562831325	27.465599944982838
51	19.962634175974692	21.369010436068336	28.635287779885495	30.03306760807148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	69.5
2	114.0
3	442.5
4	771.0
5	500.0
6	229.0
7	280.5
8	332.0
9	376.0
10	420.0
11	439.0
12	458.0
13	471.0
14	484.0
15	507.0
16	530.0
17	548.5
18	567.0
19	577.5
20	588.0
21	631.0
22	674.0
23	759.5
24	845.0
25	956.5
26	1261.5
27	1455.0
28	1646.0
29	1837.0
30	2119.5
31	2402.0
32	2656.5
33	2911.0
34	3445.0
35	3979.0
36	4266.0
37	4553.0
38	5002.5
39	5452.0
40	6179.0
41	6906.0
42	8168.5
43	9431.0
44	11266.0
45	13101.0
46	15325.5
47	17550.0
48	19668.5
49	21787.0
50	20643.5
51	19500.0
52	15932.0
53	12364.0
54	11022.0
55	9680.0
56	9012.0
57	8344.0
58	7674.0
59	7004.0
60	6371.5
61	5739.0
62	5066.5
63	4394.0
64	3802.5
65	3211.0
66	2610.0
67	2009.0
68	1672.5
69	1336.0
70	1132.5
71	929.0
72	761.0
73	593.0
74	521.0
75	338.0
76	227.0
77	168.0
78	109.0
79	84.0
80	59.0
81	53.5
82	48.0
83	33.0
84	18.0
85	12.0
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	174491.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.51324242406767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.81599999999975	42.45397502774965
2	12.35499999999996	14.211522202987073
3	6.280999999999979	10.837220269967036
4	3.3659999999999886	7.743582959976446
5	1.6899999999999942	4.859868984833702
6	0.895000000000331	3.0884611181735764
7	0.4489999999999999	1.8076412093884466
8	0.28999999999999904	1.3343072242383653
9	0.17599999999999943	0.9110097599972288
>10	0.620999999999998	6.2442127299810055
>50	0.03599999999999988	1.41482576363206
>100	0.019999999999999934	2.2648714866597772
>500	0.0019999999999999935	0.8465949284822734
>1k	0.00299999999999999	1.9819063339333653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	1276	0.7312698076118539	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGCT	1159	0.6642176387320836	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	1011	0.5793995105764768	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC	786	0.4504530319615338	TruSeq Adapter, Index 27 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	686	0.3931434859104481	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	431	0.2470041434801795	No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	426	0.2441386661776252	No Hit
TTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT	324	0.18568292920551777	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCC	267	0.1530164879563989	No Hit
GCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	241	0.1381160059831166	TruSeq Adapter, Index 27 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	239	0.1369698150620949	No Hit
CGGTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC	211	0.12092314216779088	No Hit
TGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG	190	0.10888813749706289	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC	188	0.10774194657604116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7209540893226585	0.0
2	0.0	0.0	0.0	2.6683324641385515	0.0
3	0.0	0.0	0.0	3.798476712265962	0.0
4	0.0	0.0	0.0	5.565330017020935	0.0
5	0.0	0.0	0.0	7.638789393149217	0.0
6	0.0	0.0	0.0	9.014218498375275	0.0
7	0.0	0.0	0.0	10.190783478804065	0.0
8	0.0	0.0	0.0	11.853906505206572	0.0
9	0.0	0.0	0.0	13.006401476293906	0.0
10	0.0	0.0	0.0	14.645454493354958	0.0
11	0.0	0.0	0.0	17.13841974657719	0.0
12	0.0	0.0	0.0	19.16545839040409	0.0
13	0.0	0.0	0.0	19.929394639265062	0.0
14	0.0	0.0	0.0	20.197603314784143	0.0
15	0.0	0.0	0.0	20.579284891484374	0.0
16	0.0	0.0	0.0	21.488787387315107	0.0
17	0.0	0.0	0.0	22.94215747517064	0.0
18	0.0	0.0	0.0	24.530204996246226	0.0
19	0.0	0.0	0.0	25.431111060169293	0.0
20	0.0	0.0	0.0	26.29304663277762	0.0
21	0.0	0.0	0.0	27.139508627952157	0.0
22	0.0	0.0	0.0	27.960181327403706	0.0
23	0.0	0.0	0.0	28.69145113501556	0.0
24	0.0	0.0	0.0	29.250792304474157	0.0
25	0.0	0.0	0.0	29.732192491303277	0.0
26	0.0	0.0	0.0	30.208434818987797	0.0
27	0.0	0.0	0.0	30.67608071476466	0.0
28	0.0	0.0	0.0	31.123095173963126	0.0
29	0.0	0.0	0.0	31.561513201253934	0.0
30	0.0	0.0	0.0	32.112257938804866	0.0
31	0.0	0.0	0.0	32.585634789186834	0.0
32	0.0	0.0	0.0	33.03608782114837	0.0
33	0.0	0.0	0.0	33.436108452584946	0.0
34	0.0	0.0	0.0	33.84701789777123	0.0
35	0.0	0.0	0.0	34.26480448848365	0.0
36	0.0	0.0	0.0	34.66482511992022	0.0
37	0.0	0.0	0.0	35.065418846817316	0.0
38	0.0	0.0	0.0	35.49008258305586	0.0
39	0.0	0.0	0.0	35.91875798751798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.0168247E-4	45.000004	6
ACACGAC	45	3.8016879E-10	45.000004	26
CACGGGA	20	7.0168247E-4	45.000004	4
TTAGCGG	20	7.0168247E-4	45.000004	2
GACACGA	45	3.8016879E-10	45.000004	25
TAGCGGG	20	7.0168247E-4	45.000004	3
AGTGACG	20	7.0168247E-4	45.000004	11
GCATAGA	20	7.0168247E-4	45.000004	40
TCTTATG	35	1.2040255E-7	45.0	1
TATTCAC	25	3.8771686E-5	45.0	22
ATGCGAA	25	3.8771686E-5	45.0	26
TAATGCG	25	3.8771686E-5	45.0	24
TACGGGA	30	2.1548913E-6	44.999996	4
AATGCGA	30	2.1548913E-6	44.999996	25
CCCCGAT	30	2.1548913E-6	44.999996	40
GCGATTC	30	2.1548913E-6	44.999996	9
CCCGATC	30	2.1548913E-6	44.999996	41
TTGTAGG	65	0.0	41.538464	2
ATGGGAC	60	3.6379788E-12	41.249996	5
CGACCAA	50	1.0695658E-9	40.5	29
>>END_MODULE