FastQCFastQC Report
Sat 14 Jan 2017
SRR2933206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851350
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.32954330623598993No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC44970.24290382693709997TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT35360.1909957598509196TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC24850.13422637534771922TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC23600.1274745456018581TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG253.8917322E-545.038
TAACGGG556.184564E-1140.9090923
TACGAAT2750.040.90909212
TTGGGAC39300.037.9580155
CGTTTTT35650.037.868161
TACGGGA3700.037.7027054
CTAACGG301.14019116E-437.5000042
CGCTAAT301.14019116E-437.50000444
TTTGGGA104200.037.2264864
TATGGGA10700.037.2196274
CGTTTGG12900.036.976742
TTTGGGC56400.036.7819184
TTGGGAT54800.036.5419735
TGGGCGA38800.036.2435576
ATAACGC504.8818038E-836.011
CCTATCG250.002107440336.032
TATCGTG250.002107440336.01
GCTACGA3300.035.45454410
TCGTTCA4200.035.35714316
TCGTTTG6550.035.0381661
Produced by FastQC (version 0.11.5)