##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933206.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1851350
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.356952494125906	33.0	31.0	34.0	31.0	34.0
2	32.54424068922678	34.0	31.0	34.0	31.0	34.0
3	32.61101898614525	34.0	31.0	34.0	31.0	34.0
4	36.052262943257624	37.0	35.0	37.0	35.0	37.0
5	35.24996570070489	37.0	35.0	37.0	35.0	37.0
6	34.93362357198801	37.0	35.0	37.0	32.0	37.0
7	35.72628946444487	37.0	35.0	37.0	35.0	37.0
8	36.01632970535015	37.0	35.0	37.0	35.0	37.0
9	37.89947119669431	39.0	38.0	39.0	35.0	39.0
10	37.42927269289977	39.0	37.0	39.0	35.0	39.0
11	37.35760120992789	39.0	37.0	39.0	34.0	39.0
12	37.231720096146056	39.0	37.0	39.0	34.0	39.0
13	37.22273206038837	39.0	37.0	39.0	34.0	39.0
14	38.45275069543846	40.0	38.0	41.0	34.0	41.0
15	38.52564344937478	40.0	38.0	41.0	34.0	41.0
16	38.50595511383585	40.0	38.0	41.0	34.0	41.0
17	38.455794960434275	40.0	38.0	41.0	34.0	41.0
18	38.39446025873011	40.0	38.0	41.0	34.0	41.0
19	38.38924676587355	40.0	37.0	41.0	34.0	41.0
20	38.31929294838901	40.0	37.0	41.0	34.0	41.0
21	38.22076322683447	40.0	37.0	41.0	34.0	41.0
22	38.23597861020337	40.0	37.0	41.0	34.0	41.0
23	38.162550031058416	40.0	37.0	41.0	34.0	41.0
24	38.11287600939855	40.0	37.0	41.0	34.0	41.0
25	38.009425014178845	40.0	37.0	41.0	34.0	41.0
26	37.970208226429364	40.0	37.0	41.0	34.0	41.0
27	37.93098711750884	40.0	37.0	41.0	33.0	41.0
28	37.841053825586734	40.0	36.0	41.0	33.0	41.0
29	37.770381073270855	40.0	36.0	41.0	33.0	41.0
30	37.68389337510465	40.0	36.0	41.0	33.0	41.0
31	37.625281551300404	40.0	36.0	41.0	33.0	41.0
32	37.57369217057823	40.0	36.0	41.0	33.0	41.0
33	37.47650039160612	40.0	36.0	41.0	33.0	41.0
34	37.35204688470576	40.0	36.0	41.0	33.0	41.0
35	37.218112188403055	40.0	35.0	41.0	32.0	41.0
36	37.09503119345342	39.0	35.0	41.0	32.0	41.0
37	37.07487617144246	39.0	35.0	41.0	32.0	41.0
38	36.96766683771302	39.0	35.0	41.0	31.0	41.0
39	36.92177870202825	39.0	35.0	41.0	31.0	41.0
40	36.820184189915466	39.0	35.0	41.0	31.0	41.0
41	36.71176168741729	39.0	35.0	41.0	31.0	41.0
42	36.642099548977775	39.0	35.0	41.0	31.0	41.0
43	36.52902152483323	39.0	35.0	41.0	31.0	41.0
44	36.439579766116616	39.0	35.0	41.0	31.0	41.0
45	36.43202905987523	39.0	35.0	41.0	31.0	41.0
46	36.23926270019175	38.0	35.0	40.0	30.0	41.0
47	36.164672266184134	38.0	35.0	40.0	30.0	41.0
48	36.07874578010641	38.0	35.0	40.0	30.0	41.0
49	36.09997785399843	38.0	35.0	40.0	30.0	41.0
50	35.92693277878305	38.0	35.0	40.0	30.0	41.0
51	34.874086477435384	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	24.0
10	38.0
11	38.0
12	35.0
13	38.0
14	40.0
15	60.0
16	89.0
17	179.0
18	356.0
19	582.0
20	1116.0
21	1738.0
22	2624.0
23	3922.0
24	6077.0
25	9037.0
26	12494.0
27	15524.0
28	18245.0
29	21242.0
30	26514.0
31	34072.0
32	44620.0
33	63284.0
34	107963.0
35	139677.0
36	156639.0
37	235480.0
38	391009.0
39	558354.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.848921057606614	4.298646933318929	54.99997299268101	16.852459016393443
2	20.6073405892997	4.269262970264942	53.69454722229724	21.428849218138115
3	21.172549760985227	4.201204526426661	52.33159586247873	22.29464985010938
4	18.733032651848653	4.791314446214924	51.63615739865504	24.839495503281388
5	17.36403165257785	7.674345747697626	50.31339292948389	24.648229670240635
6	21.51089745320982	7.596240581197504	53.40054554784347	17.49231641774921
7	85.00467226618413	2.063845302076863	8.848569962459827	4.082912469279175
8	87.47659815810084	2.425095200799417	6.57055662084425	3.5277500202554894
9	81.84524806222487	5.069813919572204	8.515083587652255	4.569854430550679
10	37.64053258433036	29.791557512085774	16.23977097793502	16.32813892564885
11	29.45310179058525	25.497555837631996	25.098819780160426	19.95052259162233
12	25.989899262700188	22.796607880735678	30.762092527074834	20.451400329489292
13	23.832392578388745	22.94801091095687	32.06119858481649	21.158397925837903
14	21.488751451643395	24.464525886515247	31.387311961541574	22.65941070029978
15	20.15205120587679	23.965970778080862	33.6670537715721	22.214924244470254
16	22.353417776217356	22.44842952440111	32.43870688956707	22.75944580981446
17	22.516325924325493	22.591028168633702	30.2030950387555	24.689550868285306
18	23.26226807464823	22.496934669295378	30.360871796256784	23.879925459799605
19	24.50266022091987	24.43546601128906	28.66886326194399	22.393010505847087
20	27.131282577578524	23.811542928133527	28.030950387555027	21.026224106732926
21	26.85926486077727	23.885434952872227	27.609906284603124	21.645393901747372
22	24.594052988359845	23.87101304453507	27.208091392767443	24.326842574337647
23	23.744240689226782	25.149539525211335	27.111135117616875	23.99508466794501
24	23.044373025089797	23.695897588246414	29.034596375617795	24.225133011045994
25	23.503119345342586	25.187295757150185	27.20166365084938	24.107921246657842
26	22.913495557296027	25.300186350500987	27.67861290409701	24.107705188105978
27	22.646123099359926	24.492019337240393	28.898209414751395	23.963648148648282
28	21.61195884084587	24.850892591892404	30.12817673589543	23.4089718313663
29	23.373754287411888	23.792691819483082	28.81065168660707	24.02290220649796
30	24.019121181840276	24.45555945661274	28.82350717044319	22.70181219110379
31	24.28093013206579	25.88073567936911	26.881086774515893	22.957247414049206
32	23.91265833040754	26.044562076322684	27.491722256731578	22.551057336538204
33	24.295135981851082	25.05339346963027	27.163151213978985	23.48831933453966
34	23.20722715855997	24.953412374753558	28.342290760796175	23.497069705890297
35	23.292516271909687	25.09908985335026	27.81175898668539	23.796634888054662
36	23.904070002970805	26.90199043940908	26.715315850595513	22.478623707024603
37	24.010154751937776	26.892753936316744	27.344154265806033	21.75293704593945
38	23.202960002160587	26.51081643125287	27.525481405460877	22.760742161125666
39	23.280362978367137	25.891970724066223	27.17557458071137	23.652091716855267
40	23.996813136359954	25.40313825046588	28.535879223269507	22.064169389904663
41	21.418100305182705	25.701623139870904	28.724606368325816	24.155670186620572
42	21.861182380425095	25.9753693250871	27.350906095551895	24.812542198935912
43	22.367461582088747	24.42849812299133	28.723201987738676	24.480838307181248
44	23.030761336322144	24.466632457395953	28.692413644097552	23.81019256218435
45	23.480271153482594	24.21562643476382	27.440678423852866	24.86342398790072
46	22.702946498501095	25.33324330893672	28.020093445323685	23.943716747238504
47	22.047532881410863	25.713290301671755	29.329516298917007	22.909660518000376
48	22.07313581980717	24.481378453560914	29.186269479028816	24.2592162476031
49	22.765819537094554	24.61781942906528	29.084127798633432	23.53223323520674
50	22.300483431009805	24.27023523374835	28.90112620520161	24.52815513004024
51	21.364679828233452	24.139033678126772	28.12261322818484	26.37367326545494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	1288.0
2	2300.0
3	6800.0
4	11300.0
5	7722.0
6	4144.0
7	4008.5
8	3873.0
9	3938.0
10	4003.0
11	4095.5
12	4188.0
13	4232.0
14	4276.0
15	4218.0
16	4160.0
17	3938.0
18	3716.0
19	4046.0
20	4376.0
21	4747.0
22	5118.0
23	6315.5
24	7513.0
25	9011.5
26	13127.5
27	15745.0
28	19649.0
29	23553.0
30	25615.0
31	27677.0
32	32684.5
33	37692.0
34	44839.0
35	51986.0
36	54760.5
37	57535.0
38	63328.5
39	69122.0
40	76939.0
41	84756.0
42	92077.5
43	99399.0
44	109182.0
45	118965.0
46	129449.0
47	139933.0
48	153729.5
49	167526.0
50	165158.5
51	162791.0
52	147658.5
53	132526.0
54	121292.5
55	110059.0
56	104302.0
57	98545.0
58	94653.0
59	90761.0
60	86135.5
61	81510.0
62	73410.0
63	65310.0
64	56554.0
65	47798.0
66	41243.0
67	34688.0
68	28733.0
69	22778.0
70	19210.5
71	15643.0
72	12808.0
73	9973.0
74	8524.5
75	5569.0
76	4062.0
77	3187.5
78	2313.0
79	1641.0
80	969.0
81	746.5
82	524.0
83	375.5
84	227.0
85	157.0
86	87.0
87	66.5
88	46.0
89	28.5
90	11.0
91	9.5
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1851350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.67499025864129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43476628060422	21.630911882592084
2	9.888666675491962	5.671148411813655
3	3.219425455948763	2.7695098076325775
4	1.5194844892010957	1.7428481170399184
5	0.9263392415149875	1.3281384363319713
6	0.6485522882702278	1.115833832902098
7	0.4941056574058434	0.9917932438996475
8	0.39986147711112174	0.9172819168773867
9	0.342351679728076	0.8835237973108857
>10	6.48281267945917	48.032779166633915
>50	0.575235858072016	9.907292586521645
>100	0.06367459624227426	3.074932594099039
>500	0.0032120622461702946	0.6758402459084923
>1k	0.0013226138660701213	0.9276143057719495
>5k	1.889448380100173E-4	0.33055165466471953
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6101	0.32954330623598993	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	4497	0.24290382693709997	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	3536	0.1909957598509196	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	2485	0.13422637534771922	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	2360	0.1274745456018581	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.160585518675561E-4	0.0	0.0	0.20266292165176764	0.0
2	2.160585518675561E-4	0.0	0.0	0.7554487266049099	0.0
3	2.160585518675561E-4	0.0	0.0	1.0904475112755556	0.0
4	2.160585518675561E-4	0.0	0.0	1.4498068976692684	0.0
5	2.160585518675561E-4	0.0	0.0	2.028195641018716	0.0
6	2.160585518675561E-4	0.0	0.0	2.6124719799065548	0.0
7	2.160585518675561E-4	0.0	0.0	3.1045993464228805	0.0
8	2.160585518675561E-4	0.0	0.0	3.876414508331758	0.0
9	2.160585518675561E-4	0.0	0.0	4.320360817781618	0.0
10	2.160585518675561E-4	0.0	0.0	4.973505820077241	0.0
11	2.160585518675561E-4	0.0	0.0	6.071461366030194	0.0
12	2.7007318983444516E-4	0.0	0.0	6.992356928727685	0.0
13	2.7007318983444516E-4	0.0	0.0	7.294838901342263	0.0
14	2.7007318983444516E-4	0.0	0.0	7.410214168039539	0.0
15	2.7007318983444516E-4	0.0	0.0	7.576957355443325	0.0
16	2.7007318983444516E-4	0.0	0.0	7.966294865908662	0.0
17	2.7007318983444516E-4	0.0	0.0	8.520377022173008	0.0
18	2.7007318983444516E-4	0.0	0.0	9.10686796121749	0.0
19	2.7007318983444516E-4	0.0	0.0	9.492910578766846	0.0
20	3.240878278013342E-4	0.0	0.0	9.923191184811083	0.0
21	3.240878278013342E-4	0.0	0.0	10.534906959786102	0.0
22	3.240878278013342E-4	0.0	0.0	11.20236584114295	0.0
23	3.240878278013342E-4	0.0	0.0	11.838064115375266	0.0
24	3.240878278013342E-4	0.0	0.0	12.365733113673805	0.0
25	3.240878278013342E-4	0.0	0.0	12.8181057066465	0.0
26	3.240878278013342E-4	0.0	0.0	13.213924973667863	0.0
27	3.240878278013342E-4	0.0	0.0	13.62443622221622	0.0
28	3.240878278013342E-4	0.0	0.0	14.055635077105896	0.0
29	3.781024657682232E-4	0.0	0.0	14.519026656223836	0.0
30	3.781024657682232E-4	0.0	0.0	15.0442109811759	0.0
31	4.321171037351122E-4	0.0	0.0	15.509925189726415	0.0
32	4.321171037351122E-4	0.0	0.0	15.980608744969887	0.0
33	4.321171037351122E-4	0.0	0.0	16.434655791719557	0.0
34	4.321171037351122E-4	0.0	0.0	16.903448834634187	0.0
35	4.321171037351122E-4	0.0	0.0	17.408917816728334	0.0
36	4.321171037351122E-4	0.0	0.0	17.872633483674075	0.0
37	4.321171037351122E-4	0.0	0.0	18.354822156804495	0.0
38	5.401463796688903E-4	0.0	0.0	18.81902395549194	0.0
39	5.401463796688903E-4	0.0	0.0	19.29802576498231	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAG	25	3.8917322E-5	45.0	38
TAACGGG	55	6.184564E-11	40.909092	3
TACGAAT	275	0.0	40.909092	12
TTGGGAC	3930	0.0	37.958015	5
CGTTTTT	3565	0.0	37.86816	1
TACGGGA	370	0.0	37.702705	4
CTAACGG	30	1.14019116E-4	37.500004	2
CGCTAAT	30	1.14019116E-4	37.500004	44
TTTGGGA	10420	0.0	37.226486	4
TATGGGA	1070	0.0	37.219627	4
CGTTTGG	1290	0.0	36.97674	2
TTTGGGC	5640	0.0	36.781918	4
TTGGGAT	5480	0.0	36.541973	5
TGGGCGA	3880	0.0	36.243557	6
ATAACGC	50	4.8818038E-8	36.0	11
CCTATCG	25	0.0021074403	36.0	32
TATCGTG	25	0.0021074403	36.0	1
GCTACGA	330	0.0	35.454544	10
TCGTTCA	420	0.0	35.357143	16
TCGTTTG	655	0.0	35.038166	1
>>END_MODULE