FastQCFastQC Report
Sat 14 Jan 2017
SRR2933205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913522
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.3150734613973605No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC35860.18740312366411258No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC34110.17825768399840713TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT32770.17125489019723839TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC29240.1528072319001297TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC26100.13639770015709252TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC22490.11753196461812303No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG22140.11570287668498194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA302.1662618E-645.00000424
GCTATCG207.0344633E-445.032
GCGATAT750.042.0000049
ACGGGTA403.459827E-739.3755
GGCGATA1950.039.230778
TCGTTTG6750.038.01
TTTGGGA101850.037.8424154
CGTGATA301.1402056E-437.50000444
TATGGGA11400.037.4999964
TTGGGAT45750.036.9836045
ACATACG4700.036.86170217
CGTTTTT35200.036.818181
CGTTTGG13950.036.7741932
TTATGCG800.036.56251
TACGGGA3100.036.290324
TTTGGGC50700.036.213024
TTGGGAC40300.036.1786585
ACGATTC250.002107458236.011
CTCGCAA504.8818038E-836.039
TTACACG10450.035.95693634
Produced by FastQC (version 0.11.5)