FastQCFastQC Report
Sat 14 Jan 2017
SRR2933204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816497
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250581.3794682842856332No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT71640.3943854572839922Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC66330.3651533693697265No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC65430.36019877819781704No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG63830.35139061611442246No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC53060.2921006750905727TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC48400.266446903022686TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC37480.20633119680351797TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG30870.16994247719649413No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC22620.12452539145399084No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCT21140.11637784152685085No Hit
TCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG18770.10333075144082263No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTT18520.10195447611529224No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTAGACT18320.10085345585486791No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGC207.0343685E-445.00000421
CGTTTTT190250.043.8173451
CGTTATT6200.040.645161
GTTTTTT218700.038.580252
TTATTCG356.2502895E-638.571431
CGTTCAT1750.038.57142617
CTAGCGG659.094947E-1238.0769272
CCGATGA3050.037.6229518
CGTTTGG9600.037.265632
GCTACGA1950.036.92307710
TGGGCGA36100.036.6481976
TCGTTCA1850.036.48648516
TTGGGCG27450.036.3934445
TCGCACT250.0021074336.031
ACGGGTA751.8189894E-1236.05
GCGATAG504.881622E-836.09
CGTACCG250.0021074336.012
CGATGAA3250.036.019
TTTCTTG35600.035.962081
TTTGGGA84700.035.8618664
Produced by FastQC (version 0.11.5)