##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933202.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507413
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1363445556184	33.0	31.0	34.0	30.0	34.0
2	32.33388777977702	34.0	31.0	34.0	30.0	34.0
3	32.38609377371096	34.0	31.0	34.0	30.0	34.0
4	35.90147276478923	37.0	35.0	37.0	35.0	37.0
5	35.075112383797816	37.0	35.0	37.0	33.0	37.0
6	34.776113343568255	37.0	35.0	37.0	32.0	37.0
7	35.55663138311395	37.0	35.0	37.0	33.0	37.0
8	35.82491185681092	37.0	35.0	37.0	35.0	37.0
9	37.6552689820718	39.0	37.0	39.0	35.0	39.0
10	37.240823550047004	39.0	37.0	39.0	34.0	39.0
11	37.192415251481535	39.0	37.0	39.0	34.0	39.0
12	37.01501144038485	39.0	37.0	39.0	33.0	39.0
13	36.92860253876034	39.0	37.0	39.0	33.0	39.0
14	37.87550181016253	40.0	37.0	41.0	33.0	41.0
15	37.998858917686384	40.0	37.0	41.0	33.0	41.0
16	38.04392280055891	40.0	37.0	41.0	33.0	41.0
17	38.02139677146624	40.0	37.0	41.0	33.0	41.0
18	37.958871767179794	40.0	37.0	41.0	33.0	41.0
19	37.93654872855051	40.0	37.0	41.0	33.0	41.0
20	37.78162561857895	40.0	36.0	41.0	33.0	41.0
21	37.66201299533122	39.0	36.0	41.0	33.0	41.0
22	37.721566061571146	39.0	36.0	41.0	33.0	41.0
23	37.64476471828668	39.0	35.0	41.0	33.0	41.0
24	37.563359630123784	39.0	35.0	41.0	33.0	41.0
25	37.43559979740369	39.0	35.0	41.0	33.0	41.0
26	37.384875830930625	39.0	35.0	41.0	33.0	41.0
27	37.306166771446534	39.0	35.0	41.0	32.0	41.0
28	37.21183532940622	39.0	35.0	41.0	32.0	41.0
29	37.07186453638358	39.0	35.0	41.0	32.0	41.0
30	36.93963694268771	39.0	35.0	41.0	31.0	41.0
31	36.81226338308242	39.0	35.0	41.0	31.0	41.0
32	36.72491638960768	39.0	35.0	41.0	31.0	41.0
33	36.517330064464254	39.0	35.0	41.0	30.0	41.0
34	36.32721274385954	39.0	35.0	41.0	30.0	41.0
35	36.17676724876974	39.0	35.0	40.0	30.0	41.0
36	36.01075652377846	38.0	35.0	40.0	29.0	41.0
37	35.97418670786913	38.0	35.0	40.0	29.0	41.0
38	35.85537225100658	38.0	35.0	40.0	29.0	41.0
39	35.77866944678201	38.0	35.0	40.0	29.0	41.0
40	35.67583605465371	38.0	35.0	40.0	28.0	41.0
41	35.545945807458615	38.0	35.0	40.0	27.0	41.0
42	35.47322201047273	38.0	35.0	40.0	27.0	41.0
43	35.325342472502676	38.0	34.0	40.0	27.0	41.0
44	35.268786964464844	38.0	34.0	40.0	27.0	41.0
45	35.266333341873384	38.0	34.0	40.0	27.0	41.0
46	35.08184654315124	37.0	34.0	40.0	26.0	41.0
47	34.984089883388876	37.0	34.0	40.0	26.0	41.0
48	34.88819955342098	37.0	34.0	40.0	26.0	41.0
49	34.943489819929724	37.0	34.0	40.0	26.0	41.0
50	34.74578499171287	37.0	34.0	40.0	26.0	41.0
51	33.704455739210466	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	13.0
11	17.0
12	11.0
13	13.0
14	13.0
15	18.0
16	51.0
17	84.0
18	203.0
19	312.0
20	580.0
21	886.0
22	1360.0
23	1842.0
24	2654.0
25	4270.0
26	5910.0
27	6921.0
28	7502.0
29	8555.0
30	10010.0
31	12354.0
32	16055.0
33	22303.0
34	34864.0
35	39136.0
36	46251.0
37	67381.0
38	104032.0
39	113750.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.99088513695944	4.519986677519102	51.951369003159165	18.537759182362297
2	23.679330249717687	4.211559420038509	49.29022315155505	22.818887178688758
3	21.184912487460906	4.648678689745829	51.53257799859286	22.633830824200405
4	19.021191810221655	4.889705230256221	50.57063969586905	25.51846326365308
5	17.377166134884206	7.877409526362154	50.081885958775196	24.66353837997844
6	21.73101595741536	7.444625975290345	52.97755477293644	17.846803294357848
7	79.36178221685294	2.229938925490675	13.196942135893247	5.21133672176314
8	80.55449899785776	2.8176258787220667	11.20921221963174	5.418662903788433
9	74.02924245141531	5.843760408188202	13.767483292702396	6.359513847694087
10	36.22670290276363	26.261447775283646	20.911762213423778	16.60008710852895
11	29.82245232187587	22.730202024780603	27.680607315933965	19.766738337409564
12	25.638286760488988	20.73537729620644	33.81939366945664	19.80694227384793
13	24.72266181591721	21.026461679144997	34.838287548801475	19.41258895613632
14	20.467548131403806	22.837018365709984	36.15733140459547	20.538102098290743
15	19.06514023093614	22.14350046214819	36.78206904434849	22.00929026256718
16	22.452321875868375	22.25327297487451	32.68402662131243	22.61037852794469
17	22.555787888761223	21.869758953751678	31.031723664943545	24.54272949254355
18	24.057720239725825	20.584021300203187	32.44792703379692	22.910331426274062
19	23.84645249530461	23.24832040172404	29.974596630358306	22.93063047261304
20	24.79873397015843	25.426230703588597	29.774562338765463	20.000472987487512
21	24.6942825666666	24.659399739462724	30.29110409075053	20.35521360312014
22	22.617473340257344	22.599736309475713	31.007285978088856	23.775504372178087
23	22.055209464479624	24.345454294627846	30.260754060301963	23.338582180590564
24	22.510262843088373	24.301702952033153	29.718197996503836	23.469836208374637
25	23.487967395395863	24.592590256851913	27.178649344813792	24.740793002938435
26	21.924152514815347	23.98342178856277	27.970706308273535	26.121719388348346
27	22.23021483485839	23.191167746983226	30.15847051612789	24.420146902030496
28	20.891069010845207	24.356490669336416	28.998468703009184	25.753971616809185
29	21.688250005419647	25.432734281541858	28.352643704438	24.52637200860049
30	23.962334429744605	24.24474737541214	28.629341384631452	23.1635768102118
31	24.502131399865593	25.06143910384637	27.21629126569481	23.220138230593225
32	24.655261098947012	24.635553286967422	26.75118690297647	23.957998711109095
33	24.055355302288273	24.597714287966607	27.156182439157057	24.19074797058806
34	21.934006420805144	23.766044622427884	29.53925106372915	24.760697893037822
35	22.052844527042073	25.348779002508802	27.9772098862268	24.62116658422232
36	23.75027837284421	27.161109392151957	26.426599239672615	22.662012995331217
37	23.114307280262825	27.037935567279515	27.10651875296849	22.741238399489173
38	23.72958517026564	26.02436279716917	28.196951989799235	22.04910004276595
39	23.046315328933236	24.212623641885404	28.05328204046802	24.687778988713337
40	24.7906537672468	23.6881987651085	28.328994330062496	23.192153137582206
41	21.63622138179353	23.92745160254073	27.402333010782144	27.033994004883592
42	22.69906368185285	23.90577300936318	28.950184563659185	24.44497874512478
43	22.93871067552467	23.062278656636703	30.050668784599527	23.948341883239095
44	23.599710689320137	23.271772697979753	28.27125044096229	24.857266171737816
45	22.656494807976934	22.653735714299792	27.413763541730308	27.27600593599297
46	22.433008220128375	23.58473275221565	28.44329963954412	25.538959388111852
47	20.37255647766218	24.9398419039323	30.645450550143572	24.042151068261948
48	20.696749984726445	25.2287584275531	29.794270150745056	24.280221436975403
49	22.85692325580937	23.20634276220751	30.1630033128832	23.773730669099923
50	21.591287570480063	22.885105426940186	28.987432328300617	26.53617467427914
51	20.741092561680524	22.832682647074474	27.96006408980456	28.466160701440447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	245.5
2	415.0
3	3452.0
4	6489.0
5	4189.0
6	1889.0
7	1968.0
8	2047.0
9	2090.5
10	2134.0
11	2145.0
12	2156.0
13	2109.0
14	2062.0
15	2037.0
16	2012.0
17	1939.0
18	1866.0
19	1904.5
20	1943.0
21	2000.0
22	2057.0
23	2230.5
24	2404.0
25	2705.5
26	3407.5
27	3808.0
28	4394.0
29	4980.0
30	5722.5
31	6465.0
32	7191.0
33	7917.0
34	9135.5
35	10354.0
36	11199.0
37	12044.0
38	13119.0
39	14194.0
40	15897.5
41	17601.0
42	20192.0
43	22783.0
44	26562.5
45	30342.0
46	35404.5
47	40467.0
48	46126.0
49	51785.0
50	50223.0
51	48661.0
52	43693.0
53	38725.0
54	35962.0
55	33199.0
56	31887.5
57	30576.0
58	28558.0
59	26540.0
60	23762.0
61	20984.0
62	19009.5
63	17035.0
64	14942.5
65	12850.0
66	10916.5
67	8983.0
68	7366.5
69	5750.0
70	4894.5
71	4039.0
72	3400.0
73	2761.0
74	2301.0
75	1520.0
76	1199.0
77	869.0
78	539.0
79	380.0
80	221.0
81	174.5
82	128.0
83	91.0
84	54.0
85	36.0
86	18.0
87	11.5
88	5.0
89	5.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.91354805016581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.09685022146834	34.29232595180628
2	8.486968654984912	7.963076235917716
3	4.659362946109934	6.5576174236647145
4	3.5542338902741992	6.669668895716256
5	2.770894691949583	6.499625063636307
6	2.146424044517211	6.041782052909364
7	1.5840255845673457	5.201858226200451
8	1.109455287127975	4.163878713775101
9	0.7665771550187109	3.236656877651673
>10	1.7360894561186841	11.809893514267712
>50	0.05038918793333373	1.6393209523615715
>100	0.034980923807603974	3.168455151746536
>500	0.0012493187074144275	0.38643509003296633
>1k	0.002498637414828855	2.369405850313355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4081	0.804275806887092	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	2096	0.41307573909221873	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	1913	0.377010443169568	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	1600	0.3153249916734494	No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	1421	0.28004800822998227	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC	1017	0.2004284478324363	No Hit
TCCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG	771	0.15194723036264346	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	628	0.12376505923182891	No Hit
TTCCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT	579	0.11410823136182953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.970781197959059E-4	0.0	0.0	0.44539655073874734	0.0
2	1.970781197959059E-4	0.0	0.0	1.5255817253401076	0.0
3	1.970781197959059E-4	0.0	0.0	2.2545736904651634	0.0
4	1.970781197959059E-4	0.0	0.0	3.2178915400275514	0.0
5	1.970781197959059E-4	0.0	0.0	4.661291689412766	0.0
6	1.970781197959059E-4	0.0	0.0	5.781089566093104	0.0
7	1.970781197959059E-4	0.0	0.0	6.737509681462635	0.0
8	1.970781197959059E-4	0.0	0.0	8.059312630933777	0.0
9	1.970781197959059E-4	0.0	0.0	8.996222012443512	0.0
10	1.970781197959059E-4	0.0	0.0	10.277821025476289	0.0
11	1.970781197959059E-4	0.0	0.0	12.087983555801685	0.0
12	1.970781197959059E-4	0.0	0.0	13.518179471160574	0.0
13	1.970781197959059E-4	0.0	0.0	14.088326471730129	0.0
14	1.970781197959059E-4	0.0	0.0	14.351622839777459	0.0
15	1.970781197959059E-4	0.0	0.0	14.656699769221522	0.0
16	1.970781197959059E-4	0.0	0.0	15.26862733118781	0.0
17	1.970781197959059E-4	0.0	0.0	16.13163241777408	0.0
18	1.970781197959059E-4	0.0	0.0	17.067359330565044	0.0
19	1.970781197959059E-4	0.0	0.0	17.63790048737419	0.0
20	3.941562395918118E-4	0.0	0.0	18.21750723769395	0.0
21	3.941562395918118E-4	0.0	0.0	18.86293808002554	0.0
22	3.941562395918118E-4	0.0	0.0	19.46855914215836	0.0
23	3.941562395918118E-4	0.0	0.0	20.043633095722814	0.0
24	3.941562395918118E-4	0.0	0.0	20.505584208524418	0.0
25	3.941562395918118E-4	0.0	0.0	20.924572291210513	0.0
26	3.941562395918118E-4	0.0	0.0	21.316363593364773	0.0
27	3.941562395918118E-4	0.0	0.0	21.742249410243726	0.0
28	5.912343593877177E-4	0.0	0.0	22.153354368137986	0.0
29	5.912343593877177E-4	0.0	0.0	22.585743762970203	0.0
30	5.912343593877177E-4	0.0	0.0	23.124358264372415	0.0
31	5.912343593877177E-4	0.0	0.0	23.536842769105245	0.0
32	5.912343593877177E-4	0.0	0.0	23.95977241418726	0.0
33	5.912343593877177E-4	0.0	0.0	24.361811778570907	0.0
34	5.912343593877177E-4	0.0	0.0	24.800310595116798	0.0
35	5.912343593877177E-4	0.0	0.0	25.269750676470647	0.0
36	5.912343593877177E-4	0.0	0.0	25.681249790604497	0.0
37	5.912343593877177E-4	0.0	0.0	26.087624873623657	0.0
38	5.912343593877177E-4	0.0	0.0	26.55568540813893	0.0
39	5.912343593877177E-4	0.0	0.0	27.22200653116889	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAACG	20	7.0295564E-4	45.000004	17
GGTTCGC	20	7.0295564E-4	45.000004	11
TCGTTAG	25	3.8877148E-5	45.0	1
CGTTTTT	2110	0.0	42.8673	1
TAGGGCG	75	0.0	42.000004	5
CACCGCA	65	0.0	41.538464	34
CGTTCAT	115	0.0	41.086956	17
TGTACGG	50	1.0786607E-9	40.5	2
TCGTTTG	155	0.0	39.193546	1
GTACGGG	65	9.094947E-12	38.076927	3
TTTGGGC	1140	0.0	37.894737	4
TTACACG	535	0.0	37.850468	34
TCGTTCA	125	0.0	37.800003	16
CTTGGGA	1130	0.0	37.234516	4
ACACGCG	540	0.0	37.083332	36
AGGCACG	110	0.0	36.81818	10
TTGGGAA	1030	0.0	36.699028	5
TACGGGA	105	0.0	36.428574	4
TACACGC	550	0.0	36.409092	35
TTTTGCG	340	0.0	36.397057	1
>>END_MODULE