FastQCFastQC Report
Sat 14 Jan 2017
SRR2933201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076491
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271161.3058568517754232No Hit
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT45210.2177230722406213TruSeq Adapter, Index 4 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC39440.18993580997943166RNA PCR Primer, Index 4 (96% over 26bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT36670.17659599776738738RNA PCR Primer, Index 4 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT36030.17351387509023639RNA PCR Primer, Index 4 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG32950.15868115970644708TruSeq Adapter, Index 4 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC29210.1406700053118458RNA PCR Primer, Index 48 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCC28820.1387918368054569No Hit
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC28320.13638392846393266RNA PCR Primer, Index 48 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC27060.13031599944329159TruSeq Adapter, Index 4 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC24440.1176985597337046RNA PCR Primer, Index 4 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT23110.11129352354525013TruSeq Adapter, Index 4 (95% over 24bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGT21300.1025768953489324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA406.8193913E-945.010
CGATAAG207.0346036E-445.037
CGTTTTT363350.044.3002621
CGTTATT9450.042.3809551
TATCGCG451.929584E-840.01
GTTTTTT402950.039.9633942
TAGCGCG356.2507243E-638.5714261
GTTCGCG1000.038.251
TTGGGCG28000.037.928575
CGTTTAT5400.037.9166641
TCTTGCG6350.037.9133871
CTTCGCG1700.037.0588231
TGGGCGA32300.037.0588236
GCGATCT7300.036.678089
AGCGCGG800.036.56252
GCCGATT3950.036.4556969
TCGTGCG2100.036.428571
GGGCGAT50350.036.107257
CGACGAT250.00210749936.042
CGTGCGG3250.036.02
Produced by FastQC (version 0.11.5)