##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933201.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2076491
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.104299994558126	33.0	31.0	34.0	30.0	34.0
2	32.369777186609525	34.0	31.0	34.0	30.0	34.0
3	32.290027743919914	33.0	31.0	34.0	30.0	34.0
4	35.93469945210454	37.0	35.0	37.0	35.0	37.0
5	35.189696463890286	37.0	35.0	37.0	35.0	37.0
6	34.96720717787845	37.0	35.0	37.0	33.0	37.0
7	35.75106080401986	37.0	35.0	37.0	35.0	37.0
8	35.99752804129659	37.0	35.0	37.0	35.0	37.0
9	37.86836061413221	39.0	38.0	39.0	35.0	39.0
10	37.30809813285971	39.0	37.0	39.0	34.0	39.0
11	37.27084297499965	39.0	37.0	39.0	34.0	39.0
12	37.28006189287601	39.0	37.0	39.0	34.0	39.0
13	37.34597501265356	39.0	37.0	39.0	34.0	39.0
14	38.62860951480165	40.0	38.0	41.0	34.0	41.0
15	38.675491008629464	40.0	38.0	41.0	34.0	41.0
16	38.629444577414496	40.0	38.0	41.0	34.0	41.0
17	38.55995426900478	40.0	38.0	41.0	34.0	41.0
18	38.259492095077704	39.0	38.0	41.0	34.0	41.0
19	37.9723268725942	39.0	37.0	41.0	34.0	41.0
20	37.60492340202775	39.0	35.0	41.0	34.0	41.0
21	37.47631557276193	39.0	35.0	41.0	33.0	41.0
22	37.52301117606578	39.0	35.0	41.0	33.0	41.0
23	37.443064766473825	39.0	35.0	41.0	33.0	41.0
24	37.362550090513274	39.0	35.0	41.0	33.0	41.0
25	37.27502840127889	39.0	35.0	41.0	33.0	41.0
26	37.261568193649765	39.0	35.0	41.0	33.0	41.0
27	37.21747553926311	39.0	35.0	41.0	33.0	41.0
28	37.09917403928069	39.0	35.0	41.0	33.0	41.0
29	36.96635333358054	39.0	35.0	41.0	33.0	41.0
30	36.71410856102916	39.0	35.0	41.0	31.0	41.0
31	36.3393633779294	39.0	35.0	41.0	30.0	41.0
32	35.80244027062963	39.0	35.0	41.0	27.0	41.0
33	34.975615112225384	39.0	35.0	41.0	21.0	41.0
34	34.262975375284555	39.0	34.0	41.0	15.0	41.0
35	33.79239303228379	38.0	33.0	41.0	10.0	41.0
36	33.51523507686766	38.0	33.0	40.0	10.0	41.0
37	33.40820499583191	38.0	33.0	40.0	10.0	41.0
38	33.33175294282518	38.0	33.0	40.0	10.0	41.0
39	33.25467675997633	38.0	33.0	40.0	10.0	41.0
40	33.180460209073864	38.0	33.0	40.0	10.0	41.0
41	33.068524737164765	38.0	33.0	40.0	10.0	41.0
42	32.973734054228984	38.0	32.0	40.0	9.0	41.0
43	32.84714886797005	38.0	32.0	40.0	8.0	41.0
44	32.77159929900972	38.0	32.0	40.0	8.0	41.0
45	32.78963019825272	38.0	32.0	40.0	8.0	41.0
46	32.579421244782665	38.0	31.0	40.0	8.0	41.0
47	32.49524847446967	38.0	31.0	40.0	8.0	41.0
48	32.379192108224885	38.0	31.0	40.0	8.0	41.0
49	32.41583613894787	38.0	31.0	40.0	8.0	41.0
50	32.227532890823994	37.0	31.0	40.0	8.0	41.0
51	31.327614711549437	36.0	28.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	9.0
10	8.0
11	9.0
12	6.0
13	9.0
14	18.0
15	40.0
16	140.0
17	368.0
18	815.0
19	1954.0
20	3400.0
21	5911.0
22	9466.0
23	16183.0
24	30837.0
25	59347.0
26	83390.0
27	75069.0
28	54111.0
29	41685.0
30	39057.0
31	42863.0
32	50087.0
33	64751.0
34	94689.0
35	122997.0
36	149256.0
37	224302.0
38	389743.0
39	515777.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.16584709493082	3.8771658533554922	47.089777899350395	28.867209152363287
2	32.654800815414085	3.7613936202950073	44.14755469684194	19.43625086744898
3	15.54155544136719	3.828429788523042	61.19655707633696	19.433457693772812
4	13.742270012246621	3.6641622814642587	61.713486839095374	20.880080867193744
5	13.31607023579683	6.706121047478655	61.566604430262394	18.411204286462112
6	15.876495491673213	6.403784076116873	63.84395598150919	13.875764450700725
7	70.09209286242994	1.1380737985380145	25.019805046109038	3.7500282929230124
8	72.75904398333535	1.46352668997843	22.36532689041272	3.4121024362735017
9	67.37380513568323	3.8793329708628645	24.311398412032606	4.435463481421301
10	29.347394233830055	16.559330139162654	37.076250270287716	17.017025356719582
11	22.189790372315603	23.36268252547206	36.21262986451663	18.23489723769571
12	15.791111061882763	20.098377503201313	44.57139472311703	19.539116711798897
13	17.01789220372253	20.62243467465065	46.116019766037994	16.243653355588826
14	17.28907084114499	22.095689314328837	44.592439842021946	16.022800002504226
15	16.26975508201095	22.950304142902617	44.766531615114154	16.01340915997228
16	15.947239838747192	21.600767833811947	44.071609267750254	18.3803830596906
17	16.90072338382396	22.644740574363194	42.29909977938744	18.155436262425408
18	18.06817366412857	21.30430615880348	42.746970730910945	17.880549446157
19	18.517681993324313	24.49772235949975	39.88579772317819	17.098797923997743
20	19.042365220942443	22.821095781296428	40.60234308744897	17.534195910312157
21	18.699141965941582	23.899164503963657	40.491242196571044	16.910451333523717
22	18.375422768507065	21.964650942383088	40.10799950493404	19.551926784175805
23	16.852276267992494	23.53672614039743	39.50231424070704	20.108683350903036
24	16.974068271906788	22.61285986792141	40.880023077393545	19.533048782778252
25	17.235181852461675	24.021293615045767	39.66542595176189	19.078098580730664
26	17.446933312015318	24.075616027230552	40.10809582126771	18.36935483948642
27	18.51334775830957	23.291215805895618	39.64881138420537	18.546625051589434
28	18.818815010515337	22.53493995398969	39.91430735794184	18.73193767755314
29	19.315903608539596	22.023211273248958	38.45689675515088	20.203988363060567
30	19.459029680359798	23.021337438977582	38.33240789389407	19.187224986768545
31	18.82416056703352	24.22206501256206	37.40411107006965	19.54966335033477
32	18.899672572623718	23.50185962761216	37.24143278251628	20.35703501724785
33	19.248096909642275	24.351995746670703	34.58777331565607	21.812134028030943
34	19.298759301147946	25.599533058414416	32.73517679585416	22.366530844583483
35	19.231386025752098	26.268642628357163	31.667799186223295	22.832172159667437
36	20.18641063216744	26.880299505271154	30.6455457789126	22.287744083648807
37	18.929000896223485	28.189238479723727	31.35323004048657	21.52853058356622
38	19.231963923754066	27.33809103916174	31.72843031826288	21.70151471882132
39	19.38842980778631	26.56698247187202	31.161753169168566	22.882834551173108
40	19.794788419501938	26.580948340252856	31.54591086597534	22.078352374269862
41	18.96117055166625	26.056891168803524	32.10213769286744	22.87980058666279
42	19.97090283560102	26.042973458589515	31.850607587511814	22.135516118297645
43	20.260766841753707	25.518964445307013	32.053160837200835	22.167107875738445
44	20.405241342245162	25.81768955415651	30.89394560342424	22.88312350017409
45	20.707241206439132	25.554264381593754	30.303382003582	23.43511240838511
46	21.34191768709809	26.01354881865609	29.947107885370077	22.697425608875744
47	20.421711435301187	25.789709659227995	31.143934647441284	22.64464425802953
48	20.18949275484459	25.174344603468064	31.12775350338624	23.508409138301108
49	20.6154035822934	24.375304299416662	31.367340383367903	23.64195173492204
50	20.193778831692505	23.888762339928274	31.65966045602894	24.25779837235028
51	19.13208388574764	23.826204881215475	31.372300674551447	25.669410558485446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	472.5
2	823.0
3	24774.0
4	48725.0
5	36097.0
6	23469.0
7	24312.5
8	25156.0
9	26318.0
10	27480.0
11	28338.5
12	29197.0
13	29717.0
14	30237.0
15	29843.0
16	29449.0
17	28902.5
18	28356.0
19	27603.0
20	26850.0
21	26147.5
22	25445.0
23	25435.0
24	25425.0
25	25266.5
26	25628.0
27	26148.0
28	27902.5
29	29657.0
30	33034.0
31	36411.0
32	40212.5
33	44014.0
34	50266.0
35	56518.0
36	62688.5
37	68859.0
38	75015.0
39	81171.0
40	89842.0
41	98513.0
42	109290.0
43	120067.0
44	135435.5
45	150804.0
46	173397.0
47	195990.0
48	201814.5
49	207639.0
50	196261.0
51	184883.0
52	159079.0
53	133275.0
54	114793.5
55	96312.0
56	82584.0
57	68856.0
58	58015.5
59	47175.0
60	40051.5
61	32928.0
62	27283.0
63	21638.0
64	17374.0
65	13110.0
66	10201.5
67	7293.0
68	5752.0
69	4211.0
70	3275.0
71	2339.0
72	1856.0
73	1373.0
74	1057.5
75	552.0
76	362.0
77	272.0
78	182.0
79	141.0
80	100.0
81	76.5
82	53.0
83	30.5
84	8.0
85	7.5
86	7.0
87	6.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2076491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.4328021386093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5301257516601	33.6735061155335
2	7.658789813951376	6.6528540522109285
3	3.2584687091172753	4.245732801538208
4	2.091764419868305	3.6340476067489176
5	1.473813866032512	3.200593301626446
6	1.2440131026948238	3.2418584968309037
7	0.9952371312909519	3.025815618304993
8	0.8544393834855785	2.968855734589155
9	0.7265125173649316	2.8399026976140624
>10	4.059485459763676	27.4872939404811
>50	0.06888485832186007	2.066519996582146
>100	0.034304679351304664	2.8168784213666966
>500	0.0019114924501381397	0.5324118251461009
>1k	0.0021363739148602713	2.2894880549141745
>5k	0.0	0.0
>10k+	1.1244073236106692E-4	1.3242413365127184
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27116	1.3058568517754232	No Hit
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT	4521	0.2177230722406213	TruSeq Adapter, Index 4 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	3944	0.18993580997943166	RNA PCR Primer, Index 4 (96% over 26bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT	3667	0.17659599776738738	RNA PCR Primer, Index 4 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT	3603	0.17351387509023639	RNA PCR Primer, Index 4 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG	3295	0.15868115970644708	TruSeq Adapter, Index 4 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	2921	0.1406700053118458	RNA PCR Primer, Index 48 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCC	2882	0.1387918368054569	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	2832	0.13638392846393266	RNA PCR Primer, Index 48 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	2706	0.13031599944329159	TruSeq Adapter, Index 4 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	2444	0.1176985597337046	RNA PCR Primer, Index 4 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	2311	0.11129352354525013	TruSeq Adapter, Index 4 (95% over 24bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGT	2130	0.1025768953489324	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3710716781339286E-4	0.0	0.0	0.2356860684683921	0.0
2	3.3710716781339286E-4	0.0	0.0	0.7575279642435243	0.0
3	3.3710716781339286E-4	0.0	0.0	1.1954783333999521	0.0
4	3.3710716781339286E-4	0.0	0.0	1.7354276999033467	0.0
5	3.3710716781339286E-4	0.0	0.0	2.7679387967489384	0.0
6	3.3710716781339286E-4	0.0	0.0	4.228720471218031	0.0
7	3.3710716781339286E-4	0.0	0.0	5.433782279817249	0.0
8	3.3710716781339286E-4	0.0	0.0	6.951101642145331	0.0
9	3.3710716781339286E-4	0.0	0.0	7.999745724879135	0.0
10	3.3710716781339286E-4	0.0	0.0	9.222915004206616	0.0
11	3.3710716781339286E-4	0.0	0.0	10.608955203754796	0.0
12	3.3710716781339286E-4	0.0	0.0	11.861741755683024	0.0
13	3.3710716781339286E-4	0.0	0.0	12.435305522634097	0.0
14	3.3710716781339286E-4	0.0	0.0	12.692470133508886	0.0
15	3.3710716781339286E-4	0.0	0.0	12.918091145109706	0.0
16	3.3710716781339286E-4	0.0	0.0	13.32189737398332	0.0
17	3.3710716781339286E-4	0.0	0.0	13.894642452098275	0.0
18	3.3710716781339286E-4	0.0	0.0	14.559562261526777	0.0
19	3.3710716781339286E-4	0.0	0.0	15.00897427438886	0.0
20	3.3710716781339286E-4	0.0	0.0	15.456459960577725	0.0
21	3.3710716781339286E-4	0.0	0.0	16.00830439428825	0.0
22	3.3710716781339286E-4	0.0	0.0	16.60228722397545	0.0
23	3.3710716781339286E-4	0.0	0.0	17.195258732159203	0.0
24	3.3710716781339286E-4	0.0	0.0	17.67183195111368	0.0
25	3.3710716781339286E-4	0.0	0.0	18.10390702391679	0.0
26	3.3710716781339286E-4	0.0	0.0	18.491628425069024	0.0
27	3.3710716781339286E-4	0.0	0.0	18.886380918578507	0.0
28	3.3710716781339286E-4	0.0	0.0	19.30424933216662	0.0
29	3.3710716781339286E-4	0.0	0.0	19.759921906716666	0.0
30	3.3710716781339286E-4	0.0	0.0	20.261392897922505	0.0
31	3.3710716781339286E-4	0.0	0.0	20.73083870818607	0.0
32	3.3710716781339286E-4	0.0	0.0	21.18781155324054	0.0
33	3.3710716781339286E-4	0.0	0.0	21.63972779077781	0.0
34	3.3710716781339286E-4	0.0	0.0	22.063904924220715	0.0
35	3.852653346438776E-4	0.0	0.0	22.50243319137911	0.0
36	3.852653346438776E-4	0.0	0.0	22.937397754192048	0.0
37	3.852653346438776E-4	0.0	0.0	23.401979589605734	0.0
38	3.852653346438776E-4	0.0	0.0	23.848020530789682	0.0
39	3.852653346438776E-4	0.0	0.0	24.302392834835306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCTA	40	6.8193913E-9	45.0	10
CGATAAG	20	7.0346036E-4	45.0	37
CGTTTTT	36335	0.0	44.300262	1
CGTTATT	945	0.0	42.380955	1
TATCGCG	45	1.929584E-8	40.0	1
GTTTTTT	40295	0.0	39.963394	2
TAGCGCG	35	6.2507243E-6	38.571426	1
GTTCGCG	100	0.0	38.25	1
TTGGGCG	2800	0.0	37.92857	5
CGTTTAT	540	0.0	37.916664	1
TCTTGCG	635	0.0	37.913387	1
CTTCGCG	170	0.0	37.058823	1
TGGGCGA	3230	0.0	37.058823	6
GCGATCT	730	0.0	36.67808	9
AGCGCGG	80	0.0	36.5625	2
GCCGATT	395	0.0	36.455696	9
TCGTGCG	210	0.0	36.42857	1
GGGCGAT	5035	0.0	36.10725	7
CGACGAT	25	0.002107499	36.0	42
CGTGCGG	325	0.0	36.0	2
>>END_MODULE