SRR2933200.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	650869
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	3962	0.6087246435150545	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3958	0.608110080523116	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3716	0.5709290195108385	RNA PCR Primer, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3584	0.5506484407768691	RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC	2860	0.439412539236006	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	2716	0.41728827152622106	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	2531	0.38886473314906683	RNA PCR Primer, Index 10 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1538	0.2362994704003417	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1356	0.2083368542671413	TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	911	0.13996672141398653	RNA PCR Primer, Index 35 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	857	0.1316701210228172	TruSeq Adapter, Index 10 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	832	0.12782910232320174	RNA PCR Primer, Index 35 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT	719	0.11046769780093998	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	678	0.10416842713357065	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC	662	0.10171017516581679	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	25	3.888934E-5	45.000004	40
CACTAAC	25	3.888934E-5	45.000004	26
TACAACG	25	3.888934E-5	45.000004	39
CAGCGTT	20	7.03103E-4	45.0	29
TCGCATA	20	7.03103E-4	45.0	43
TCGCAAT	20	7.03103E-4	45.0	31
TAGGTTA	20	7.03103E-4	45.0	38
CCGGATA	20	7.03103E-4	45.0	5
TTATCCG	20	7.03103E-4	45.0	1
TCTAGCG	40	6.8066583E-9	45.0	1
CGGATCA	20	7.03103E-4	45.0	17
AACGATT	20	7.03103E-4	45.0	28
AGACACG	40	6.8066583E-9	45.0	24
GCGATAT	20	7.03103E-4	45.0	9
CGTGTTA	20	7.03103E-4	45.0	43
CGTAAGT	20	7.03103E-4	45.0	36
CGGTCAT	20	7.03103E-4	45.0	27
CGACTCG	20	7.03103E-4	45.0	16
TATCGCA	20	7.03103E-4	45.0	29
CAATAGG	40	6.8066583E-9	45.0	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content