##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	650869
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.971579534437804	33.0	31.0	34.0	30.0	34.0
2	32.14432704584179	33.0	31.0	34.0	30.0	34.0
3	32.239372285360034	34.0	31.0	34.0	30.0	34.0
4	35.79870603762048	37.0	35.0	37.0	35.0	37.0
5	35.0059689430592	37.0	35.0	37.0	33.0	37.0
6	34.69262017395206	37.0	35.0	37.0	32.0	37.0
7	35.543580966369575	37.0	35.0	37.0	33.0	37.0
8	35.77598257099355	37.0	35.0	37.0	35.0	37.0
9	37.62758404533017	39.0	37.0	39.0	35.0	39.0
10	37.07857648774177	39.0	37.0	39.0	33.0	39.0
11	36.97911254031149	39.0	37.0	39.0	33.0	39.0
12	36.811591887153945	39.0	35.0	39.0	33.0	39.0
13	36.66579449935394	39.0	35.0	39.0	33.0	39.0
14	37.68876532758512	39.0	36.0	41.0	33.0	41.0
15	37.82622770480696	40.0	36.0	41.0	33.0	41.0
16	37.887326021058	40.0	36.0	41.0	33.0	41.0
17	37.8307908350221	40.0	36.0	41.0	33.0	41.0
18	37.80048366107466	39.0	36.0	41.0	33.0	41.0
19	37.810010923857185	39.0	36.0	41.0	33.0	41.0
20	37.69562231416767	39.0	36.0	41.0	33.0	41.0
21	37.57750791633954	39.0	36.0	41.0	33.0	41.0
22	37.64929655583535	39.0	36.0	41.0	33.0	41.0
23	37.54286346407649	39.0	35.0	41.0	33.0	41.0
24	37.47083207219886	39.0	35.0	41.0	33.0	41.0
25	37.30486165418848	39.0	35.0	41.0	32.0	41.0
26	37.28246697876224	39.0	35.0	41.0	32.0	41.0
27	37.25076167400813	39.0	35.0	41.0	32.0	41.0
28	37.151396056656566	39.0	35.0	41.0	32.0	41.0
29	37.14309945626539	39.0	35.0	40.0	32.0	41.0
30	37.09334597284553	39.0	35.0	40.0	32.0	41.0
31	36.99219044077994	39.0	35.0	40.0	31.0	41.0
32	36.97809543855983	39.0	35.0	40.0	31.0	41.0
33	36.86475619517906	39.0	35.0	40.0	31.0	41.0
34	36.80950698220379	39.0	35.0	40.0	31.0	41.0
35	36.74784326800017	39.0	35.0	40.0	31.0	41.0
36	36.632803221539206	39.0	35.0	40.0	31.0	41.0
37	36.60752470927329	39.0	35.0	40.0	31.0	41.0
38	36.527828180478714	39.0	35.0	40.0	30.0	41.0
39	36.51541247163408	39.0	35.0	40.0	30.0	41.0
40	36.4262777916908	39.0	35.0	40.0	30.0	41.0
41	36.32076808082733	38.0	35.0	40.0	30.0	41.0
42	36.28478849046429	38.0	35.0	40.0	30.0	41.0
43	36.129818750009605	38.0	35.0	40.0	30.0	41.0
44	36.047024823735654	38.0	35.0	40.0	30.0	41.0
45	36.070453501395825	38.0	35.0	40.0	30.0	41.0
46	35.835802596221356	38.0	35.0	40.0	29.0	41.0
47	35.75138007801877	38.0	35.0	40.0	29.0	41.0
48	35.72510597370592	38.0	35.0	40.0	29.0	41.0
49	35.87980991566659	38.0	35.0	40.0	30.0	41.0
50	35.661043927426256	38.0	34.0	40.0	29.0	41.0
51	34.476845571074975	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	11.0
11	10.0
12	5.0
13	7.0
14	8.0
15	15.0
16	18.0
17	43.0
18	114.0
19	180.0
20	362.0
21	709.0
22	1100.0
23	1659.0
24	2404.0
25	3561.0
26	4983.0
27	6194.0
28	7542.0
29	9293.0
30	12201.0
31	15799.0
32	20737.0
33	28743.0
34	46439.0
35	61298.0
36	59088.0
37	86062.0
38	139289.0
39	142949.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.568172704491992	4.377378550829738	53.09762794049186	15.956820804186403
2	18.94835980819489	6.697046563901492	52.28840212085688	22.066191507046735
3	19.909382686838672	6.612083230265998	51.41280349809255	22.06573058480278
4	18.180156068271803	4.327291666986752	51.8168786652921	25.67567359944935
5	17.218518626636083	8.212251620525791	50.207645470901205	24.361584281936917
6	19.265627952783127	8.590054219819963	54.52571869300888	17.618599134388027
7	79.39232011357124	2.0045508389553044	13.045789552121857	5.5573394953515995
8	80.80320310231399	3.9708451316624394	8.837108542579228	6.388843223444349
9	74.17836768996526	5.606811816202646	11.57191385670542	8.642906637126673
10	38.241335814119275	25.030843380157915	20.145989438734986	16.581831366987824
11	28.669056292433652	24.48541872481252	26.728112723143983	20.117412259609846
12	24.768424982600184	21.436418081057788	32.93473801947858	20.860418916863456
13	22.043759957840976	25.594704925261457	34.364826101719395	17.99670901517817
14	18.157878159814032	29.033184865157196	31.75277974523291	21.056157229795858
15	15.42660658289149	24.907777141022233	38.81779590055756	20.847820375528716
16	16.60226558646978	23.82691447895045	34.458700598738	25.112119335841776
17	17.56712948381318	23.99791663145733	30.534101332218928	27.900852552510568
18	19.877579052005856	23.33818325961138	33.44175248782781	23.34248520055495
19	21.13051935182041	25.25147149426382	30.885170441363773	22.732838712551988
20	23.2590582743993	25.69564689668735	30.18518319354586	20.860111635367485
21	21.7569126813537	27.73323049645935	29.45047313668342	21.059383685503537
22	20.182402296007336	24.58313424053074	28.479156327924667	26.755307135537258
23	18.85556079641218	27.183964822414342	27.66163390789852	26.29884047327496
24	21.228695789782584	23.5477492398624	30.081936610900193	25.14161835945482
25	18.809468572016797	26.455707676967254	28.400492264956544	26.33433148605941
26	17.04874560011308	27.323777903080344	28.766618167403884	26.860858329402692
27	19.253797615188308	25.610683563051857	31.00961944723132	24.125899374528515
28	16.32801685131724	23.68786960202437	33.628425996629126	26.355687550029266
29	16.91492450861848	22.217374003063597	33.04320838755571	27.82449310076221
30	18.78872707103887	23.436820619817507	33.725066027111446	24.049386282032177
31	19.992809612994318	24.40014810968106	28.017926802474847	27.58911547484978
32	19.400370888765636	25.45965470778298	29.69952478916648	25.440449614284905
33	18.700690922443684	22.504682201794832	30.203005520312075	28.59162135544941
34	16.892032037168768	21.779805152803405	30.51750813143659	30.810654678591238
35	16.298978749948144	20.83768008616173	32.52390265936771	30.339438504522416
36	19.39453254034222	21.223164722855138	30.70264523275805	28.67965750404459
37	16.755599052958427	23.271349534238073	33.85827255561411	26.114778857189386
38	16.405451788301487	24.781945368422832	31.555965947064617	27.256636896211067
39	18.73295547952046	22.020252923399333	32.71195893490088	26.53483266217933
40	19.779402614043686	20.339423140447614	33.06563993676147	26.815534308747228
41	16.523294242005687	20.9418485132953	30.014181041038977	32.52067620366003
42	18.530610614424713	20.59738595631379	30.73721440105459	30.134789028206903
43	19.706884180994948	20.85289052021221	29.923379358980075	29.516845939812775
44	18.612808414596486	21.721114387073282	30.625671218017757	29.04040598031247
45	18.636161808290147	21.59528261447388	29.735476724194882	30.03307885304109
46	21.3224166460532	21.68946439298845	31.259285662706322	25.72883329825203
47	16.513307593386685	21.961715798417192	34.449328513110935	27.075648095085185
48	17.07302083829465	20.360932845165465	33.74642209108131	28.81962422545858
49	18.473763537670408	18.57731740181204	34.968941522794914	27.979977537722643
50	19.37624929133205	19.062975806191414	32.0165809095225	29.54419399295404
51	17.53363580075253	19.06543405815917	29.587367043137714	33.81356309795059
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	317.5
2	515.0
3	1629.0
4	2743.0
5	1880.0
6	1017.0
7	1033.5
8	1050.0
9	1075.5
10	1101.0
11	1203.0
12	1305.0
13	1262.0
14	1219.0
15	1319.5
16	1420.0
17	1347.5
18	1275.0
19	1405.0
20	1535.0
21	1677.5
22	1820.0
23	2183.0
24	2546.0
25	2618.5
26	3565.0
27	4439.0
28	5117.0
29	5795.0
30	7143.5
31	8492.0
32	9352.5
33	10213.0
34	12055.5
35	13898.0
36	15145.0
37	16392.0
38	19382.5
39	22373.0
40	26814.5
41	31256.0
42	37113.0
43	42970.0
44	48634.0
45	54298.0
46	68518.5
47	82739.0
48	85905.5
49	89072.0
50	83521.0
51	77970.0
52	63207.5
53	48445.0
54	40940.0
55	33435.0
56	28424.0
57	23413.0
58	21216.5
59	19020.0
60	17115.5
61	15211.0
62	13003.5
63	10796.0
64	8879.5
65	6963.0
66	5837.0
67	4711.0
68	3948.0
69	3185.0
70	2552.0
71	1919.0
72	1787.0
73	1655.0
74	1352.0
75	727.5
76	406.0
77	322.0
78	238.0
79	154.0
80	70.0
81	63.5
82	57.0
83	38.5
84	20.0
85	12.5
86	5.0
87	3.5
88	2.0
89	3.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	650869.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.790439348877044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33278905549871	23.71823932459448
2	11.992553867160227	7.864822204385165
3	4.801147507136079	4.722952084132734
4	2.400981945371581	3.1491701142982254
5	1.444155442837492	2.3677245729356726
6	0.8888558724170449	1.7487584744630584
7	0.6383714252850363	1.4652735652005624
8	0.4692683941890718	1.2310013454401347
9	0.3939807137004032	1.1626920627498327
>10	4.066537433993986	32.718887266966014
>50	0.4785417627069301	9.76794119729397
>100	0.0848875251558818	5.176696837810557
>500	0.0037313197870717275	0.8956138230388387
>1k	0.004197734760455693	4.010227126690811
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	3962	0.6087246435150545	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3958	0.608110080523116	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3716	0.5709290195108385	RNA PCR Primer, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3584	0.5506484407768691	RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC	2860	0.439412539236006	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	2716	0.41728827152622106	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	2531	0.38886473314906683	RNA PCR Primer, Index 10 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1538	0.2362994704003417	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1356	0.2083368542671413	TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	911	0.13996672141398653	RNA PCR Primer, Index 35 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	857	0.1316701210228172	TruSeq Adapter, Index 10 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	832	0.12782910232320174	RNA PCR Primer, Index 35 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT	719	0.11046769780093998	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	678	0.10416842713357065	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC	662	0.10171017516581679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.536407479846175E-4	0.0	0.0	0.6520513344467166	0.0
2	1.536407479846175E-4	0.0	0.0	2.5857737885811125	0.0
3	3.07281495969235E-4	0.0	0.0	3.61885417802968	0.0
4	3.07281495969235E-4	0.0	0.0	4.8283141461645895	0.0
5	3.07281495969235E-4	0.0	0.0	7.032597957499896	0.0
6	3.07281495969235E-4	0.0	0.0	8.177528811481265	0.0
7	3.07281495969235E-4	0.0	0.0	9.343662088684512	0.0
8	3.07281495969235E-4	0.0	0.0	11.222995718032355	0.0
9	3.07281495969235E-4	0.0	0.0	12.241172954926414	0.0
10	3.07281495969235E-4	0.0	0.0	14.337908242672489	0.0
11	3.07281495969235E-4	0.0	0.0	17.013408228076617	0.0
12	3.07281495969235E-4	0.0	0.0	19.829489497886673	0.0
13	3.07281495969235E-4	0.0	0.0	20.59077940415045	0.0
14	3.07281495969235E-4	0.0	0.0	20.841060182617394	0.0
15	3.07281495969235E-4	0.0	0.0	21.29122757421232	0.0
16	3.07281495969235E-4	0.0	0.0	22.256552393799673	0.0
17	3.07281495969235E-4	0.0	0.0	23.741029300827048	0.0
18	3.07281495969235E-4	0.0	0.0	25.377917829855164	0.0
19	3.07281495969235E-4	0.0	0.0	26.481365681880686	0.0
20	3.07281495969235E-4	0.0	0.0	27.450838801663622	0.0
21	3.07281495969235E-4	0.0	0.0	28.73973103650658	0.0
22	3.07281495969235E-4	0.0	0.0	30.046906520359705	0.0
23	3.07281495969235E-4	0.0	0.0	31.357615741416474	0.0
24	3.07281495969235E-4	0.0	0.0	32.25241945767889	0.0
25	3.07281495969235E-4	0.0	0.0	33.01908679012213	0.0
26	3.07281495969235E-4	0.0	0.0	33.708165544833136	0.0
27	3.07281495969235E-4	0.0	0.0	34.38956226214491	0.0
28	3.07281495969235E-4	0.0	0.0	35.09569513988222	0.0
29	3.07281495969235E-4	0.0	0.0	35.90031173707766	0.0
30	3.07281495969235E-4	0.0	0.0	36.807867635422795	0.0
31	3.07281495969235E-4	0.0	0.0	37.601422098763344	0.0
32	3.07281495969235E-4	0.0	0.0	38.30863046173654	0.0
33	3.07281495969235E-4	0.0	0.0	38.97343397826598	0.0
34	3.07281495969235E-4	0.0	0.0	39.69216539733802	0.0
35	3.07281495969235E-4	0.0	0.0	40.44638782919451	0.0
36	3.07281495969235E-4	0.0	0.0	41.142687699060794	0.0
37	3.07281495969235E-4	0.0	0.0	41.865106496084465	0.0
38	3.07281495969235E-4	0.0	0.0	42.574619470277426	0.0
39	3.07281495969235E-4	0.0	0.0	43.33529481354927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	25	3.888934E-5	45.000004	40
CACTAAC	25	3.888934E-5	45.000004	26
TACAACG	25	3.888934E-5	45.000004	39
CAGCGTT	20	7.03103E-4	45.0	29
TCGCATA	20	7.03103E-4	45.0	43
TCGCAAT	20	7.03103E-4	45.0	31
TAGGTTA	20	7.03103E-4	45.0	38
CCGGATA	20	7.03103E-4	45.0	5
TTATCCG	20	7.03103E-4	45.0	1
TCTAGCG	40	6.8066583E-9	45.0	1
CGGATCA	20	7.03103E-4	45.0	17
AACGATT	20	7.03103E-4	45.0	28
AGACACG	40	6.8066583E-9	45.0	24
GCGATAT	20	7.03103E-4	45.0	9
CGTGTTA	20	7.03103E-4	45.0	43
CGTAAGT	20	7.03103E-4	45.0	36
CGGTCAT	20	7.03103E-4	45.0	27
CGACTCG	20	7.03103E-4	45.0	16
TATCGCA	20	7.03103E-4	45.0	29
CAATAGG	40	6.8066583E-9	45.0	13
>>END_MODULE