FastQCFastQC Report
Sat 14 Jan 2017
SRR2933199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536147
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.2762105449543566No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC29880.1945126345330232Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT27580.1795401091171613Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC23220.15115740876361441Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC20810.1354688060452548Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG253.8914215E-545.03
CGTAGTA207.03403E-445.04
TCTAGCG403.459245E-739.3751
TCGTCAC403.459245E-739.37515
GCGATAT800.039.3759
TTTGGGC47100.038.5031854
TACGAAT2200.037.84090812
GGCGATA1550.037.741948
TCGCTAT301.1401004E-437.50000440
TATACGG301.1401004E-437.5000042
TTTGGGA75900.037.292494
CGTTTGG8300.036.867472
GCTACGA2200.036.8181810
CGTTTTT24350.036.776181
TCAAGCG6000.036.7517
TTGGGAC27500.036.5727275
TGGGCGA29350.036.413976
ACACGAC5900.036.22881326
AGACACG6150.036.21951324
ACGTGAG5600.036.16071344
Produced by FastQC (version 0.11.5)