##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933199.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1536147
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2920788179777	33.0	31.0	34.0	31.0	34.0
2	32.47989482777364	34.0	31.0	34.0	31.0	34.0
3	32.54638390726929	34.0	31.0	34.0	31.0	34.0
4	36.00528204657497	37.0	35.0	37.0	35.0	37.0
5	35.221210600287606	37.0	35.0	37.0	35.0	37.0
6	34.913509579486856	37.0	35.0	37.0	32.0	37.0
7	35.695618322986014	37.0	35.0	37.0	35.0	37.0
8	35.98313442658808	37.0	35.0	37.0	35.0	37.0
9	37.856365959768176	39.0	38.0	39.0	35.0	39.0
10	37.372974070840876	39.0	37.0	39.0	34.0	39.0
11	37.2835132314811	39.0	37.0	39.0	34.0	39.0
12	37.08289050461968	39.0	37.0	39.0	34.0	39.0
13	37.04435057321988	39.0	37.0	39.0	33.0	39.0
14	38.213913772575154	40.0	37.0	41.0	33.0	41.0
15	38.290836749347555	40.0	37.0	41.0	34.0	41.0
16	38.271478575943576	40.0	37.0	41.0	34.0	41.0
17	38.18851190673809	40.0	37.0	41.0	33.0	41.0
18	38.145069449733654	40.0	37.0	41.0	33.0	41.0
19	38.150412688369016	40.0	37.0	41.0	34.0	41.0
20	38.08533037528309	40.0	37.0	41.0	34.0	41.0
21	37.98350027699172	40.0	36.0	41.0	33.0	41.0
22	38.00935066761189	40.0	36.0	41.0	34.0	41.0
23	37.94820287381351	40.0	36.0	41.0	34.0	41.0
24	37.89426532747191	40.0	36.0	41.0	33.0	41.0
25	37.797870255906496	40.0	36.0	41.0	33.0	41.0
26	37.761959630165606	40.0	36.0	41.0	33.0	41.0
27	37.725928573242015	40.0	36.0	41.0	33.0	41.0
28	37.646018252159465	40.0	36.0	41.0	33.0	41.0
29	37.57888730700903	40.0	36.0	41.0	33.0	41.0
30	37.4788474019739	39.0	35.0	41.0	33.0	41.0
31	37.41286999225986	39.0	35.0	41.0	33.0	41.0
32	37.39560211359981	39.0	35.0	41.0	33.0	41.0
33	37.25964377107139	39.0	35.0	41.0	32.0	41.0
34	37.064624023612325	39.0	35.0	41.0	31.0	41.0
35	36.9640665899813	39.0	35.0	41.0	31.0	41.0
36	36.874638950569185	39.0	35.0	41.0	31.0	41.0
37	36.84977479368836	39.0	35.0	41.0	31.0	41.0
38	36.753225440013225	39.0	35.0	41.0	31.0	41.0
39	36.70234033591837	39.0	35.0	41.0	31.0	41.0
40	36.58545764174913	39.0	35.0	41.0	31.0	41.0
41	36.48954624785258	39.0	35.0	41.0	31.0	41.0
42	36.4146601855161	39.0	35.0	40.0	30.0	41.0
43	36.251981743934664	38.0	35.0	40.0	30.0	41.0
44	36.16200728185519	38.0	35.0	40.0	30.0	41.0
45	36.17262280237503	38.0	35.0	40.0	30.0	41.0
46	35.991701315043414	38.0	35.0	40.0	30.0	41.0
47	35.933555187101234	38.0	35.0	40.0	30.0	41.0
48	35.85097585061846	38.0	35.0	40.0	30.0	41.0
49	35.8904525413258	38.0	35.0	40.0	30.0	41.0
50	35.71869358856932	38.0	35.0	40.0	29.0	41.0
51	34.67047684889532	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	18.0
10	36.0
11	33.0
12	25.0
13	20.0
14	32.0
15	58.0
16	82.0
17	165.0
18	278.0
19	536.0
20	935.0
21	1462.0
22	2385.0
23	3578.0
24	5178.0
25	7738.0
26	10801.0
27	13710.0
28	15862.0
29	19320.0
30	24078.0
31	31207.0
32	41083.0
33	58791.0
34	104704.0
35	127480.0
36	132195.0
37	194401.0
38	317718.0
39	422061.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.57612910743568	4.4096040287811	55.101302154025625	16.912964709757595
2	20.584488333473296	4.506925443984202	53.45106946145128	21.457516761091224
3	20.943568551707617	4.503345057471713	52.3479849259218	22.205101464898867
4	18.994731623991715	4.8149688799314125	51.26866113724793	24.92163835882894
5	17.613027919854023	7.8781522862069835	50.13296253548651	24.37585725845248
6	22.008570794331533	7.5165983463822155	52.979369812915046	17.495461046371215
7	84.43911943323133	2.2136553337668854	8.922062797375512	4.4251624356262775
8	87.04043297939586	2.5307473829002043	6.6847769126262	3.744042725077743
9	81.32581061578091	5.007723870176487	8.622677386994864	5.043788127047737
10	39.68142371791241	27.985017058914284	16.13673691385004	16.19682230932326
11	31.952801392054276	24.83759692269034	23.678593259629448	19.531008425625934
12	28.44929554267918	20.737403386524857	30.070624751407255	20.742676319388703
13	24.946961456162725	21.581658526169694	31.684532795363985	21.786847222303596
14	21.704823822199305	23.879876079567904	31.88379757926813	22.531502518964654
15	20.987770050652703	22.99799433257364	33.675878675673616	22.338356941100038
16	23.35408004572479	21.366119258117873	32.467595874613565	22.812204821543773
17	22.6873469791628	21.60607025239121	30.369749770041537	25.33683299840445
18	23.952069691246997	22.039036628655982	30.14880737325269	23.860086306844334
19	24.563860099326433	25.0647887213919	28.105969025099814	22.26538215418186
20	28.306796159482133	24.00772842703205	27.264708390538146	20.420767022947672
21	26.689437924886093	24.943446167586824	27.272455044992437	21.09466086253464
22	24.437244612657512	23.63048588448892	27.811400862026876	24.120868640826693
23	24.837271433007388	24.488281394944625	26.869563915432572	23.804883256615415
24	24.68377049852651	22.866236108914055	28.933884582660387	23.516108809899052
25	24.301580512802488	23.74037120145403	26.737740593836396	25.22030769190709
26	22.053878958198663	24.544591110095585	28.37208938988261	25.029440541823146
27	21.65541448832696	24.017948803076788	29.308197718056935	25.018438990539316
28	20.595620080630304	25.759513900687892	29.276104435317713	24.368761583364094
29	22.62628511464072	23.44586813631768	29.462284533967125	24.465562215074467
30	23.37321883908246	23.5536052213753	29.837509040475947	23.235666899066302
31	24.310173440432457	25.85722590351054	27.070260853941715	22.762339802115292
32	25.34080397253648	24.905949756110584	26.829072998873155	22.92417327247978
33	25.20891555300372	23.99783354067026	26.967080624445444	23.826170281880575
34	22.791959363264063	24.127769022105305	29.35630509319746	23.72396652143317
35	22.103353390007598	24.65356505594842	29.277927177542253	23.965154376501726
36	23.324525582512614	26.664896002791398	27.61604195431817	22.394536460377815
37	24.24012806066086	26.64621289498987	27.823574176169334	21.290084868179935
38	23.644742332602284	26.019840549114114	27.546842847722257	22.788574270561345
39	23.528998201344013	24.802248743121588	27.282545225163997	24.386207830370402
40	23.612518853989886	24.46569241094765	28.145092884990824	23.77669585007164
41	21.635364323857026	25.21301672300893	28.382635255610307	24.768983697523737
42	23.403879967216678	24.484766106368728	27.409876789135414	24.70147713727918
43	22.969806926029865	22.917988968503668	28.909472856438867	25.2027312490276
44	22.90353722658053	23.406028199124172	28.24150292908166	25.44893164521364
45	22.8631114079577	23.418852492632542	27.770779749594276	25.94725634981548
46	22.119172188599137	25.00886959386048	28.442915944893294	24.429042272647084
47	21.18996424170343	24.922289338194847	30.094320400326268	23.793426019775453
48	21.73737279049466	23.29946287692519	30.81274122854128	24.15042310403887
49	22.532088400393974	22.92091837565025	30.184611238377578	24.362381985578203
50	21.666676431357153	22.673611314542164	29.637267787522937	26.022444466577742
51	21.06308836328815	22.63246941861684	28.86618272860605	27.438259489488964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	926.0
2	1626.0
3	4962.5
4	8299.0
5	5653.0
6	3007.0
7	2962.0
8	2917.0
9	2990.5
10	3064.0
11	3158.0
12	3252.0
13	3273.5
14	3295.0
15	3190.0
16	3085.0
17	3232.0
18	3379.0
19	3531.5
20	3684.0
21	3770.5
22	3857.0
23	4894.0
24	5931.0
25	6989.5
26	10033.5
27	12019.0
28	13920.0
29	15821.0
30	18663.5
31	21506.0
32	25554.5
33	29603.0
34	34676.5
35	39750.0
36	43537.5
37	47325.0
38	49105.0
39	50885.0
40	57113.5
41	63342.0
42	72296.5
43	81251.0
44	88126.0
45	95001.0
46	105034.0
47	115067.0
48	128229.5
49	141392.0
50	139885.0
51	138378.0
52	125990.5
53	113603.0
54	104286.0
55	94969.0
56	91659.5
57	88350.0
58	84743.0
59	81136.0
60	76318.0
61	71500.0
62	65260.0
63	59020.0
64	50674.0
65	42328.0
66	35679.0
67	29030.0
68	23836.5
69	18643.0
70	15531.0
71	12419.0
72	10110.0
73	7801.0
74	6343.0
75	4320.0
76	3755.0
77	2918.0
78	2081.0
79	1559.0
80	1037.0
81	679.0
82	321.0
83	236.5
84	152.0
85	107.0
86	62.0
87	45.0
88	28.0
89	20.0
90	12.0
91	6.5
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1536147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.550209061360828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3908968535225	24.10148766113486
2	9.56937436203897	6.038315234175117
3	3.201439064361522	3.0301821533344024
4	1.50513632145241	1.8994946243068453
5	0.909600625957885	1.434904495565797
6	0.6112472411621483	1.1570986948107491
7	0.48824421021264547	1.0782944833645578
8	0.3940107096257804	0.9944896208886803
9	0.3285180203043635	0.9328330998924347
>10	6.257699495510527	48.86211442741709
>50	0.27879633728941355	5.488302158348677
>100	0.06052316772983274	3.2463754465506582
>500	0.0028722850749121082	0.5788105157779198
>1k	0.0016413057570926336	1.1572973844322776
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4243	0.2762105449543566	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2988	0.1945126345330232	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	2758	0.1795401091171613	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2322	0.15115740876361441	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2081	0.1354688060452548	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3019587318140776E-4	0.0	0.0	0.1907369542107624	0.0
2	1.3019587318140776E-4	0.0	0.0	0.7239541528252179	0.0
3	1.3019587318140776E-4	0.0	0.0	1.0861590720158942	0.0
4	1.3019587318140776E-4	0.0	0.0	1.507668211440702	0.0
5	1.3019587318140776E-4	0.0	0.0	2.227586292197296	0.0
6	1.3019587318140776E-4	0.0	0.0	2.8663923439618735	0.0
7	1.3019587318140776E-4	0.0	0.0	3.4031899290888177	0.0
8	1.3019587318140776E-4	0.0	0.0	4.2150262963114855	0.0
9	1.3019587318140776E-4	0.0	0.0	4.695318872477699	0.0
10	2.603917463628155E-4	0.0	0.0	5.485477626815663	0.0
11	2.603917463628155E-4	0.0	0.0	6.898884026073025	0.0
12	2.603917463628155E-4	0.0	0.0	8.084642941072698	0.0
13	2.603917463628155E-4	0.0	0.0	8.478745849192817	0.0
14	2.603917463628155E-4	0.0	0.0	8.621700917946004	0.0
15	2.603917463628155E-4	0.0	0.0	8.8332041139292	0.0
16	2.603917463628155E-4	0.0	0.0	9.332505287579899	0.0
17	2.603917463628155E-4	0.0	0.0	10.025537920524533	0.0
18	3.254896829535194E-4	0.0	0.0	10.763097542097208	0.0
19	4.5568555613492717E-4	0.0	0.0	11.279063787515128	6.509793659070388E-5
20	4.5568555613492717E-4	0.0	0.0	11.817228429310477	6.509793659070388E-5
21	4.5568555613492717E-4	0.0	0.0	12.563446076449715	6.509793659070388E-5
22	4.5568555613492717E-4	0.0	0.0	13.36857735620354	6.509793659070388E-5
23	5.20783492725631E-4	0.0	0.0	14.140248296549744	6.509793659070388E-5
24	5.20783492725631E-4	0.0	0.0	14.762975157976417	6.509793659070388E-5
25	5.20783492725631E-4	0.0	0.0	15.318586046778075	6.509793659070388E-5
26	5.85881429316335E-4	0.0	0.0	15.788332757216594	6.509793659070388E-5
27	5.85881429316335E-4	0.0	0.0	16.273117091007567	6.509793659070388E-5
28	5.85881429316335E-4	0.0	0.0	16.761937496867162	6.509793659070388E-5
29	5.85881429316335E-4	0.0	0.0	17.29639155627684	6.509793659070388E-5
30	5.85881429316335E-4	0.0	0.0	17.891516892589056	6.509793659070388E-5
31	5.85881429316335E-4	0.0	0.0	18.42186978199352	6.509793659070388E-5
32	6.509793659070388E-4	0.0	0.0	18.93588308931372	6.509793659070388E-5
33	6.509793659070388E-4	0.0	0.0	19.47990654540223	6.509793659070388E-5
34	6.509793659070388E-4	0.0	0.0	20.008631986391926	6.509793659070388E-5
35	7.811752390884466E-4	0.0	0.0	20.579215400609446	6.509793659070388E-5
36	7.811752390884466E-4	0.0	0.0	21.1131486765264	6.509793659070388E-5
37	7.811752390884466E-4	0.0	0.0	21.64695175657017	6.509793659070388E-5
38	8.462731756791505E-4	0.0	0.0	22.205492052518412	6.509793659070388E-5
39	8.462731756791505E-4	0.0	0.0	22.79925033216222	6.509793659070388E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	25	3.8914215E-5	45.0	3
CGTAGTA	20	7.03403E-4	45.0	4
TCTAGCG	40	3.459245E-7	39.375	1
TCGTCAC	40	3.459245E-7	39.375	15
GCGATAT	80	0.0	39.375	9
TTTGGGC	4710	0.0	38.503185	4
TACGAAT	220	0.0	37.840908	12
GGCGATA	155	0.0	37.74194	8
TCGCTAT	30	1.1401004E-4	37.500004	40
TATACGG	30	1.1401004E-4	37.500004	2
TTTGGGA	7590	0.0	37.29249	4
CGTTTGG	830	0.0	36.86747	2
GCTACGA	220	0.0	36.81818	10
CGTTTTT	2435	0.0	36.77618	1
TCAAGCG	600	0.0	36.75	17
TTGGGAC	2750	0.0	36.572727	5
TGGGCGA	2935	0.0	36.41397	6
ACACGAC	590	0.0	36.228813	26
AGACACG	615	0.0	36.219513	24
ACGTGAG	560	0.0	36.160713	44
>>END_MODULE