##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447706
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12962747874721	33.0	31.0	34.0	30.0	34.0
2	32.320518375898466	34.0	31.0	34.0	30.0	34.0
3	32.39758234198335	34.0	31.0	34.0	30.0	34.0
4	35.898232322104235	37.0	35.0	37.0	35.0	37.0
5	35.09254064050962	37.0	35.0	37.0	35.0	37.0
6	34.781771966424394	37.0	35.0	37.0	32.0	37.0
7	35.514111939531745	37.0	35.0	37.0	33.0	37.0
8	35.79611620125708	37.0	35.0	37.0	35.0	37.0
9	37.63264955126802	39.0	37.0	39.0	35.0	39.0
10	37.219742420249005	39.0	37.0	39.0	34.0	39.0
11	37.18538281818872	39.0	37.0	39.0	34.0	39.0
12	37.08093480989756	39.0	37.0	39.0	33.0	39.0
13	37.0617704475705	39.0	37.0	39.0	33.0	39.0
14	38.18222226193082	40.0	37.0	41.0	33.0	41.0
15	38.245332874699024	40.0	37.0	41.0	33.0	41.0
16	38.23107351699553	40.0	37.0	41.0	33.0	41.0
17	38.17953299710078	40.0	37.0	41.0	33.0	41.0
18	38.146917843406165	40.0	37.0	41.0	33.0	41.0
19	38.16453878214721	40.0	37.0	41.0	34.0	41.0
20	38.06993205362448	40.0	37.0	41.0	34.0	41.0
21	37.95854422321791	40.0	37.0	41.0	33.0	41.0
22	37.98693338932246	40.0	37.0	41.0	33.0	41.0
23	37.90110921006196	40.0	37.0	41.0	33.0	41.0
24	37.84855239822562	40.0	36.0	41.0	33.0	41.0
25	37.73076974621738	40.0	36.0	41.0	33.0	41.0
26	37.678570311767096	40.0	36.0	41.0	33.0	41.0
27	37.65420834208163	40.0	36.0	41.0	33.0	41.0
28	37.56642528802384	40.0	36.0	41.0	33.0	41.0
29	37.487339906099095	39.0	36.0	41.0	33.0	41.0
30	37.39198715228297	39.0	36.0	41.0	32.0	41.0
31	37.291954988318224	39.0	36.0	41.0	32.0	41.0
32	37.25041880162428	39.0	35.0	41.0	32.0	41.0
33	37.14645101919563	39.0	35.0	41.0	32.0	41.0
34	36.98541453543174	39.0	35.0	41.0	31.0	41.0
35	36.86456960594676	39.0	35.0	41.0	31.0	41.0
36	36.75490165421058	39.0	35.0	41.0	31.0	41.0
37	36.70019164362327	39.0	35.0	41.0	31.0	41.0
38	36.58325106208092	39.0	35.0	40.0	30.0	41.0
39	36.52958861395648	39.0	35.0	40.0	30.0	41.0
40	36.42288242730721	39.0	35.0	40.0	30.0	41.0
41	36.32181833614023	39.0	35.0	40.0	30.0	41.0
42	36.25376698100986	38.0	35.0	40.0	30.0	41.0
43	36.0965075294948	38.0	35.0	40.0	30.0	41.0
44	36.02656207421835	38.0	35.0	40.0	30.0	41.0
45	36.02656877504434	38.0	35.0	40.0	30.0	41.0
46	35.83978325061536	38.0	35.0	40.0	29.0	41.0
47	35.74495316122634	38.0	35.0	40.0	29.0	41.0
48	35.65793400133123	38.0	35.0	40.0	29.0	41.0
49	35.696157746378205	38.0	35.0	40.0	29.0	41.0
50	35.49524911437417	38.0	34.0	40.0	28.0	41.0
51	34.44441441481687	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	18.0
11	13.0
12	5.0
13	8.0
14	13.0
15	19.0
16	32.0
17	58.0
18	108.0
19	220.0
20	380.0
21	635.0
22	951.0
23	1337.0
24	1704.0
25	2349.0
26	3272.0
27	3981.0
28	4712.0
29	5849.0
30	7484.0
31	9502.0
32	12617.0
33	17340.0
34	28241.0
35	35870.0
36	42067.0
37	61836.0
38	97276.0
39	109756.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.377368183584764	4.391721352852095	53.772341670649936	16.458568792913205
2	21.047517790693	4.683654005083693	51.86260626393213	22.406221940291175
3	21.582913787172835	4.526184594354331	50.65288381214458	23.23801780632826
4	19.120806958137706	4.888699280331289	50.08889762478055	25.901596136750456
5	17.309573693450613	8.07248506832609	49.20349515083559	25.414446087387706
6	21.710452841820302	7.745931481820659	52.43530352508119	18.108312151277847
7	80.65560881471323	2.368965347795205	11.235274934890308	5.74015090260126
8	82.0844482763242	2.892969940094616	9.042988032324786	5.979593751256405
9	75.21610163812859	6.164089826805984	11.799931204853186	6.819877330212237
10	36.19116116379946	27.070443550008267	19.045981067932974	17.6924142182593
11	26.78543508463143	24.90607675572809	26.669957516763233	21.638530642877246
12	22.712002966232305	22.005959267912427	34.271821239831496	21.010216526023775
13	23.499796741611682	22.434365409442805	34.06565916025249	20.000178688693026
14	20.475937333875354	25.56945852858796	32.61828074674005	21.33632339079664
15	18.367634117032157	23.94160453511903	35.08217446270543	22.608586885143374
16	20.620228453494033	23.558317288577772	30.87048196807726	24.950972289850927
17	20.79891714652026	23.219702215293072	29.221408692311474	26.759971945875193
18	22.00863959830782	22.38745962752342	31.152140020459857	24.451760753708907
19	23.001478648934793	23.753311324842645	29.665003372749084	23.580206653473486
20	25.225036072779904	24.114039123889338	28.63307617052262	22.027848632808137
21	25.230396733570693	24.63581010752592	28.563610941108674	21.570182217794713
22	22.982716336167037	23.929989770072325	27.891294733597494	25.19599916016314
23	22.668224236440878	24.39257012414397	27.116232527596235	25.822973111818914
24	23.646321469893188	23.806247850151664	27.66771050644843	24.87972017350672
25	22.026285106744158	24.753074562324382	27.08339848025267	26.137241850678794
26	21.56325803093995	24.953429259380037	28.193278624811818	25.290034084868196
27	22.150697109263668	23.98717015184072	28.49124202043305	25.37089071846256
28	20.17373008179475	24.000795164683968	29.258486596114412	26.56698815740687
29	21.41807346785614	24.3662135419226	28.12269659106646	26.0930163991548
30	23.256780119096014	24.79841681817979	26.9882467512162	24.956556311508
31	22.801347312745417	28.02106739690779	24.122080115075516	25.05550517527127
32	22.622211897986624	28.521842459113795	24.29138765171787	24.56455799118171
33	23.690100199684615	25.826100163946876	24.826336926465135	25.657462709903374
34	21.561247783143404	25.70816562654957	26.706812059699892	26.02377453060714
35	22.16923606116514	26.09189959482339	26.475410202230925	25.263454141780546
36	24.75910530571402	25.650315162182324	26.35032811711257	23.240251414991086
37	22.92665275873006	27.49862633067236	25.82721696827829	23.74750394231929
38	21.24251182695787	29.567841395916073	24.64541462477608	24.54423215234998
39	22.950999093154884	27.307206068268012	25.25585987232693	24.485934966250174
40	22.69011360133659	26.580166448517556	26.57837956158729	24.151340388558562
41	20.98274313947099	27.00298856839086	25.822526390086352	26.191741902051792
42	23.638280478707006	25.296734910856678	25.766909534381938	25.298075076054378
43	24.440369349528485	23.707522347254674	26.779851062974362	25.07225724024248
44	22.340553845604035	23.45869834221565	26.970377881913578	27.23036993026674
45	22.75019767436666	23.910780735572004	26.152653750452302	27.186367839609026
46	22.842892433874017	24.5699186519725	28.126940447525833	24.460248466627654
47	20.16568909060857	26.440119185358245	28.8850272276896	24.509164496343583
48	20.39776103067638	25.325548462607156	29.179193488584026	25.097497018132437
49	22.553640112037808	23.162968555257244	29.688009541976207	24.595381790728737
50	21.93716412109733	23.143089438158075	27.98733990609909	26.9324065346455
51	21.113632607112702	22.672914814632815	27.402357797304482	28.811094780949997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	236.5
2	354.0
3	1246.5
4	2139.0
5	1485.5
6	832.0
7	887.5
8	943.0
9	988.5
10	1034.0
11	1105.5
12	1177.0
13	1164.5
14	1152.0
15	1185.5
16	1219.0
17	1227.5
18	1236.0
19	1420.5
20	1605.0
21	1647.5
22	1690.0
23	1814.5
24	1939.0
25	2264.0
26	2877.0
27	3165.0
28	3630.0
29	4095.0
30	4751.5
31	5408.0
32	6173.5
33	6939.0
34	8049.5
35	9160.0
36	10420.0
37	11680.0
38	12498.0
39	13316.0
40	14870.0
41	16424.0
42	19277.5
43	22131.0
44	25615.5
45	29100.0
46	34317.0
47	39534.0
48	45644.5
49	51755.0
50	49408.5
51	47062.0
52	40349.0
53	33636.0
54	30218.5
55	26801.0
56	25570.5
57	24340.0
58	22550.0
59	20760.0
60	18935.5
61	17111.0
62	15796.5
63	14482.0
64	13238.5
65	11995.0
66	9755.5
67	7516.0
68	6121.5
69	4727.0
70	4041.5
71	3356.0
72	2759.0
73	2162.0
74	1834.0
75	1113.5
76	721.0
77	592.0
78	463.0
79	320.5
80	178.0
81	134.5
82	91.0
83	68.0
84	45.0
85	26.0
86	7.0
87	8.0
88	9.0
89	6.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	447706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.16201611910587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91256900831684	32.641280639498135
2	7.978504921372842	7.046937258880659
3	3.7135335003783276	4.9199137890764195
4	2.7930923192829895	4.933943521053047
5	2.289351597953526	5.0551191085562195
6	2.0242247548127583	5.36363077504405
7	1.6965075835095373	5.244483667439348
8	1.3797843463234267	4.87472468345801
9	1.1050669299016291	4.392178521390592
>10	3.0192861136222606	18.035254155653703
>50	0.051840738435259125	1.625023441702547
>100	0.030701796548999842	2.6359120496694515
>500	0.0030198488408852303	0.8875281907690463
>1k	0.002516540700737692	2.344070197808756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	3172	0.7085006678489902	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	2584	0.5771644784747133	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1916	0.42795941979781377	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1712	0.38239380307612586	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1162	0.25954532662059476	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	828	0.18494279728214497	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	738	0.16484031931669443	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	660	0.1474181717466373	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	636	0.1420575109558505	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	569	0.127092332914904	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	562	0.1255288068509245	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	499	0.1114570722751091	No Hit
TTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	469	0.10475624628662558	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.233608662827838E-4	0.0	0.0	0.763447440954555	0.0
2	2.233608662827838E-4	0.0	0.0	2.3220595658758203	0.0
3	2.233608662827838E-4	0.0	0.0	3.2550379043390083	0.0
4	2.233608662827838E-4	0.0	0.0	4.421651708933988	0.0
5	2.233608662827838E-4	0.0	0.0	6.085690162740727	0.0
6	2.233608662827838E-4	0.0	0.0	7.119404251897451	0.0
7	2.233608662827838E-4	0.0	0.0	8.01954854301707	0.0
8	2.233608662827838E-4	0.0	0.0	9.297396059020876	0.0
9	2.233608662827838E-4	0.0	0.0	10.108866086226229	0.0
10	2.233608662827838E-4	0.0	0.0	11.364600876468039	0.0
11	2.233608662827838E-4	0.0	0.0	13.362340464501257	0.0
12	2.233608662827838E-4	0.0	0.0	15.040003931151247	0.0
13	2.233608662827838E-4	0.0	0.0	15.65893689162084	0.0
14	2.233608662827838E-4	0.0	0.0	15.904857205398185	0.0
15	2.233608662827838E-4	0.0	0.0	16.237664896159533	0.0
16	2.233608662827838E-4	0.0	0.0	17.061196410144156	2.233608662827838E-4
17	2.233608662827838E-4	0.0	0.0	18.336586956618852	2.233608662827838E-4
18	2.233608662827838E-4	0.0	0.0	19.664020584937436	2.233608662827838E-4
19	2.233608662827838E-4	0.0	0.0	20.45159099945053	2.233608662827838E-4
20	2.233608662827838E-4	0.0	0.0	21.23134378364373	2.233608662827838E-4
21	2.233608662827838E-4	0.0	0.0	22.0633630105471	2.233608662827838E-4
22	2.233608662827838E-4	0.0	0.0	22.830830947094746	2.233608662827838E-4
23	2.233608662827838E-4	0.0	0.0	23.550499658257873	2.233608662827838E-4
24	2.233608662827838E-4	0.0	0.0	24.133024797523376	2.233608662827838E-4
25	2.233608662827838E-4	0.0	0.0	24.619058042554713	2.233608662827838E-4
26	2.233608662827838E-4	0.0	0.0	25.054835092672423	2.233608662827838E-4
27	2.233608662827838E-4	0.0	0.0	25.505130599098514	2.233608662827838E-4
28	2.233608662827838E-4	0.0	0.0	26.003225330909125	2.233608662827838E-4
29	2.233608662827838E-4	0.0	0.0	26.504670475713972	2.233608662827838E-4
30	2.233608662827838E-4	0.0	0.0	27.030908676676212	2.233608662827838E-4
31	2.233608662827838E-4	0.0	0.0	27.480534100503455	2.233608662827838E-4
32	2.233608662827838E-4	0.0	0.0	27.928819359133	2.233608662827838E-4
33	2.233608662827838E-4	0.0	0.0	28.371297235239197	2.233608662827838E-4
34	2.233608662827838E-4	0.0	0.0	28.824943154659532	2.233608662827838E-4
35	2.233608662827838E-4	0.0	0.0	29.29266080865568	2.233608662827838E-4
36	2.233608662827838E-4	0.0	0.0	29.73960590208753	2.233608662827838E-4
37	2.233608662827838E-4	0.0	0.0	30.164214908891104	2.233608662827838E-4
38	2.233608662827838E-4	0.0	0.0	30.594407937351743	2.233608662827838E-4
39	2.233608662827838E-4	0.0	0.0	31.023930883213538	2.233608662827838E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	30	2.162522E-6	45.000004	26
CGTATGG	35	1.2097007E-7	45.000004	2
ACGTTAC	25	3.8869766E-5	45.000004	36
AAGCGTT	25	3.8869766E-5	45.000004	30
TAGTTCG	25	3.8869766E-5	45.000004	1
ACGCCTA	20	7.028669E-4	45.0	38
CGTTGGC	20	7.028669E-4	45.0	30
TTGCGAG	20	7.028669E-4	45.0	1
TCGTAAC	20	7.028669E-4	45.0	34
CACGTTA	20	7.028669E-4	45.0	35
CTACGGG	75	0.0	42.0	3
CGTTTGG	255	0.0	41.47059	2
TTACACG	125	0.0	41.399998	34
CGTTTTT	775	0.0	40.35484	1
TCTACGG	45	1.9237632E-8	40.0	2
TGCGGGT	120	0.0	39.375004	4
ATACTAT	210	0.0	38.57143	45
TCGTCGG	35	6.239745E-6	38.57143	2
CGAGATA	130	0.0	38.07692	19
GCCGATT	95	0.0	37.894737	9
>>END_MODULE