FastQCFastQC Report
Sat 14 Jan 2017
SRR2933194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228748
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314211.4098049667346868No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT76130.34158191056144527Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC73510.32982643170066783No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT61170.2744590236312046No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT58070.26054986925394885No Hit
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC55360.24839057623383173Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC52550.2357826008144483Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC48510.21765583188408918No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC46220.2073810049408906Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG44060.1976894651167382No Hit
CGTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT38210.17144154475965878No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGT35470.15914764701976178No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC34250.15367372174871274No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC31770.14254639824690812No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG31480.14124521928903583No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC25030.11230520453635853No Hit
TTCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT23500.1054403638275839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT9600.044.296881
CGTTTTT318050.044.285491
CGTTTTC7900.040.443041
CGTTTCT8950.040.223461
GTTTTTT349900.040.0743062
AGCGTCG403.4601726E-739.37517
CGTTCTG8500.038.117651
TGGGCGA34150.037.8843356
TAGTGCG301.14026574E-437.5000041
CGTTTGG13400.036.772392
TTACGGG6150.036.5853653
TTGGGCG29700.036.3636365
CGTATGG1500.036.02
TCTTGCG6450.035.930231
TTTGGGC70650.035.8598754
TCGTTTG8650.035.635841
ACGGGTA1200.035.6250045
TTGTACG950.035.5263141
TCTAGCG950.035.5263141
GGGCGAT53550.035.4621857
Produced by FastQC (version 0.11.5)