##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933194.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2228748
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04396414489211	33.0	31.0	34.0	30.0	34.0
2	32.29803055347666	34.0	31.0	34.0	30.0	34.0
3	32.2419865323491	33.0	31.0	34.0	30.0	34.0
4	35.875749075265574	37.0	35.0	37.0	35.0	37.0
5	35.09598976645184	37.0	35.0	37.0	35.0	37.0
6	34.883956822395355	37.0	35.0	37.0	32.0	37.0
7	35.680775036029196	37.0	35.0	37.0	35.0	37.0
8	35.92780565591085	37.0	35.0	37.0	35.0	37.0
9	37.77975392462495	39.0	38.0	39.0	35.0	39.0
10	37.272307142844326	39.0	37.0	39.0	34.0	39.0
11	37.220905189819575	39.0	37.0	39.0	34.0	39.0
12	37.239899261827716	39.0	37.0	39.0	34.0	39.0
13	37.29813105833409	39.0	37.0	39.0	34.0	39.0
14	38.515677860395165	40.0	38.0	41.0	34.0	41.0
15	38.56172030216067	40.0	38.0	41.0	34.0	41.0
16	38.52070041117255	40.0	38.0	41.0	34.0	41.0
17	38.44908800815525	40.0	38.0	41.0	34.0	41.0
18	38.20514163108615	39.0	38.0	41.0	34.0	41.0
19	37.990925398474836	39.0	37.0	41.0	34.0	41.0
20	37.69314476109457	39.0	35.0	41.0	34.0	41.0
21	37.56660106929989	39.0	35.0	41.0	33.0	41.0
22	37.62234671663194	39.0	35.0	41.0	33.0	41.0
23	37.54465466710458	39.0	35.0	41.0	33.0	41.0
24	37.48324889130579	39.0	35.0	41.0	33.0	41.0
25	37.40363199428558	39.0	35.0	41.0	33.0	41.0
26	37.393256662484944	39.0	35.0	41.0	33.0	41.0
27	37.36353773508714	39.0	35.0	41.0	33.0	41.0
28	37.26988739866508	39.0	35.0	41.0	33.0	41.0
29	37.16791310637183	39.0	35.0	41.0	33.0	41.0
30	36.97209016003604	39.0	35.0	41.0	32.0	41.0
31	36.69211727840025	39.0	35.0	41.0	31.0	41.0
32	36.28919509967031	39.0	35.0	41.0	30.0	41.0
33	35.62949961144104	39.0	35.0	41.0	25.0	41.0
34	35.0230519556271	39.0	35.0	41.0	21.0	41.0
35	34.6369867746376	39.0	34.0	40.0	18.0	41.0
36	34.36999113403579	38.0	34.0	40.0	16.0	41.0
37	34.243091188416095	38.0	34.0	40.0	15.0	41.0
38	34.085410284159536	38.0	34.0	40.0	15.0	41.0
39	34.00491621304876	38.0	33.0	40.0	15.0	41.0
40	33.90220338952631	38.0	33.0	40.0	15.0	41.0
41	33.814164723871876	38.0	33.0	40.0	14.0	41.0
42	33.72848433290798	38.0	33.0	40.0	12.0	41.0
43	33.57780287407998	38.0	33.0	40.0	12.0	41.0
44	33.53639487281649	38.0	33.0	40.0	10.0	41.0
45	33.609206603887024	38.0	33.0	40.0	11.0	41.0
46	33.45320691258052	38.0	33.0	40.0	10.0	41.0
47	33.40124949074548	38.0	33.0	40.0	10.0	41.0
48	33.28497389565801	38.0	32.0	40.0	10.0	41.0
49	33.330416000373305	38.0	33.0	40.0	10.0	41.0
50	33.13464869065502	38.0	32.0	40.0	10.0	41.0
51	32.20118918783101	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	19.0
11	12.0
12	18.0
13	19.0
14	25.0
15	47.0
16	118.0
17	295.0
18	744.0
19	1631.0
20	3007.0
21	4984.0
22	8181.0
23	13629.0
24	24980.0
25	47327.0
26	68218.0
27	63769.0
28	50392.0
29	42445.0
30	42466.0
31	48423.0
32	59435.0
33	75727.0
34	111711.0
35	145120.0
36	181948.0
37	270700.0
38	448985.0
39	514226.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.974039236378452	3.6878552442896195	50.21909161556174	26.11901390377019
2	31.059904484490847	3.655146297383105	45.328812409478324	19.956136808647727
3	15.902336199516501	3.796570989631847	59.10632337078934	21.194769440062313
4	14.31220577651668	3.608348723139628	59.543968183033705	22.535477317309986
5	13.991128651601706	6.6678691354967	58.899705125927206	20.44129708697439
6	16.63023365584624	6.407498739202458	61.05647655096045	15.90579105399085
7	70.30444895519817	1.1626258329788741	23.537115905432106	4.995809306390853
8	72.4140189918286	1.669322866470323	20.973972831383357	4.942685310317721
9	66.15063703927048	4.295550685855916	23.618910706818358	5.934901568055249
10	29.69546130832198	19.06630987442277	33.7421727355448	17.49605608171045
11	21.296216530536427	23.020772200356436	36.33869778009896	19.344313489008176
12	16.280440857378224	19.74839685778742	43.363089950052675	20.60807233478168
13	17.67543930493712	20.004056088889367	45.201117398647135	17.11938720752638
14	17.030503224231722	22.34896004393498	43.51353315852667	17.107003573306628
15	15.353373284014163	23.088971925044913	44.06415619890629	17.49349859203463
16	15.641472252583064	22.620233422531395	42.371187769994634	19.367106554890906
17	16.385253065846832	23.62725619944471	40.25654762225249	19.73094311245596
18	17.578389301975818	22.20228576761482	40.692846387299056	19.5264785431103
19	17.845759143698615	25.40210916622247	38.09510990026688	18.65702178981204
20	18.58173288321515	24.696242015696704	37.91770087959698	18.80432422149117
21	18.474946472189767	25.661268120038695	38.091789650512304	17.771995757259234
22	17.66000463040236	23.366302516031425	37.78614720013209	21.187545653434125
23	16.835460985270654	24.366998871115083	36.98838989423658	21.809150249377677
24	17.008472918427746	23.354367564210936	38.54468966433172	21.092469853029595
25	17.38891072476565	24.636028837715166	37.072203766419534	20.90285667109965
26	17.157166265544603	24.809848399190937	38.106282092008605	19.926703243255854
27	18.067767194855588	23.60168130268653	37.53273138102648	20.797820121431403
28	18.135607973624655	22.74389029176919	37.82756058558437	21.292941149021782
29	18.436628995292427	22.26747932022822	37.36640481561845	21.929486868860902
30	18.760846897002263	23.744496910372998	36.97012852058645	20.524527672038293
31	18.129102078835295	25.705149258686937	34.93082214768112	21.234926514796648
32	18.709383025806417	24.817206790538904	34.661433235161624	21.811976948493054
33	20.314992991580922	25.65135223901491	32.36579460755545	21.667860161848715
34	19.31658491673352	26.49460594019602	31.71650630757717	22.47230283549329
35	20.67945770450495	26.926417881249918	30.612882210101816	21.781242204143314
36	21.902790266104557	28.002941561809592	29.423694379086374	20.670573792999477
37	21.91100115401113	28.279958075116614	29.429886196196247	20.379154574676008
38	22.528926554280698	27.464163736770598	29.468158804853665	20.538750904095036
39	23.43320106176203	26.455211625540436	29.38389625027145	20.72769106242608
40	22.919369978122244	26.692609482992246	30.352466945567645	20.03555359331786
41	21.71376037129366	25.97530093128519	30.810257597539064	21.500681099882087
42	22.790553261292885	25.68616999319798	30.847991787317362	20.675284958191774
43	21.80753499274032	24.560739931118277	32.24397733615465	21.387747739986754
44	21.273288859933917	24.772293682372347	30.664749895457	23.289667562236733
45	21.2115950300348	24.16029088977309	30.48428983447209	24.14382424572002
46	21.46016507922834	25.032933288106147	30.388675615188436	23.118226017477078
47	20.46821803093037	25.227706317627657	31.43293903124086	22.871136620201117
48	20.436855131221655	24.182500668536775	31.793096393131933	23.58754780710964
49	21.38806181766624	22.872123721479504	32.04579432039872	23.69402014045554
50	20.37175131508811	22.629790357635766	32.22513267538547	24.77332565189066
51	19.282170976709796	22.342140071466133	31.710718304626635	26.66497064719744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	597.0
2	1003.0
3	27429.0
4	53855.0
5	38220.0
6	22585.0
7	23050.5
8	23516.0
9	24203.5
10	24891.0
11	25295.5
12	25700.0
13	25555.0
14	25410.0
15	25013.5
16	24617.0
17	24238.5
18	23860.0
19	23322.0
20	22784.0
21	22011.0
22	21238.0
23	21152.5
24	21067.0
25	21394.5
26	22568.5
27	23415.0
28	25449.0
29	27483.0
30	30795.5
31	34108.0
32	38883.5
33	43659.0
34	49100.5
35	54542.0
36	61543.5
37	68545.0
38	76106.5
39	83668.0
40	94082.0
41	104496.0
42	118876.0
43	133256.0
44	153898.0
45	174540.0
46	208171.0
47	241802.0
48	250831.5
49	259861.0
50	237465.5
51	215070.0
52	179292.0
53	143514.0
54	120649.5
55	97785.0
56	82643.0
57	67501.0
58	57603.5
59	47706.0
60	40994.0
61	34282.0
62	28994.5
63	23707.0
64	19233.5
65	14760.0
66	11729.5
67	8699.0
68	6998.0
69	5297.0
70	4214.5
71	3132.0
72	2617.5
73	2103.0
74	1877.5
75	1297.5
76	943.0
77	665.5
78	388.0
79	296.0
80	204.0
81	163.5
82	123.0
83	84.5
84	46.0
85	28.5
86	11.0
87	9.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2228748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.382309931819684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.19553581313633	34.57929421692819
2	8.668824582992787	7.868225679399124
3	3.870870835291279	5.270071798596917
4	2.592726488612807	4.706556682986599
5	1.913236132008296	4.341353755777819
6	1.4769105720293736	4.021536799285088
7	1.1416253219598627	3.626671593103715
8	0.9079221399833171	3.29628831605471
9	0.6797746589787432	2.776476983181332
>10	2.422704219849364	16.21964811830922
>50	0.07646437024684376	2.3841112885028957
>100	0.04590722965106259	3.9514022541710965
>500	0.004498581155996618	1.4255986938510248
>1k	0.0022992748130649214	2.3978349542089488
>5k	5.998108207995446E-4	1.709420907794051
>10k+	9.996847013325744E-5	1.425507957849117
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31421	1.4098049667346868	No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	7613	0.34158191056144527	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC	7351	0.32982643170066783	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT	6117	0.2744590236312046	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT	5807	0.26054986925394885	No Hit
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	5536	0.24839057623383173	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	5255	0.2357826008144483	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	4851	0.21765583188408918	No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	4622	0.2073810049408906	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	4406	0.1976894651167382	No Hit
CGTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	3821	0.17144154475965878	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGT	3547	0.15914764701976178	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC	3425	0.15367372174871274	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	3177	0.14254639824690812	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	3148	0.14124521928903583	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	2503	0.11230520453635853	No Hit
TTCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	2350	0.1054403638275839	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.97364798532629E-5	0.0	0.0	0.38954605904301426	0.0
2	8.97364798532629E-5	0.0	0.0	1.2055647385886605	0.0
3	8.97364798532629E-5	0.0	0.0	1.9036248153671926	0.0
4	8.97364798532629E-5	0.0	0.0	2.863176994438133	0.0
5	8.97364798532629E-5	0.0	0.0	4.572477462683085	0.0
6	1.3460471977989435E-4	0.0	0.0	6.9092153980620505	0.0
7	1.3460471977989435E-4	0.0	0.0	8.772862611654615	0.0
8	1.3460471977989435E-4	0.0	0.0	11.05086802096962	0.0
9	1.3460471977989435E-4	0.0	0.0	12.60182847051349	0.0
10	1.3460471977989435E-4	0.0	0.0	14.395570966300362	0.0
11	1.3460471977989435E-4	0.0	0.0	16.600575749254737	0.0
12	1.3460471977989435E-4	0.0	0.0	18.407868453499454	0.0
13	1.3460471977989435E-4	0.0	0.0	19.218727285453536	0.0
14	1.3460471977989435E-4	0.0	0.0	19.575564397590036	0.0
15	1.3460471977989435E-4	0.0	0.0	19.896596654265085	0.0
16	1.3460471977989435E-4	0.0	0.0	20.487197296419335	0.0
17	1.3460471977989435E-4	0.0	0.0	21.30335058068476	0.0
18	1.3460471977989435E-4	0.0	0.0	22.258685145202598	0.0
19	1.3460471977989435E-4	0.0	0.0	22.878898825708426	0.0
20	1.3460471977989435E-4	0.0	0.0	23.5121691640329	0.0
21	1.3460471977989435E-4	0.0	0.0	24.2635775780842	0.0
22	1.3460471977989435E-4	0.0	0.0	25.055860958708656	0.0
23	1.3460471977989435E-4	0.0	0.0	25.868559388499733	0.0
24	1.3460471977989435E-4	0.0	0.0	26.50250275042311	0.0
25	1.3460471977989435E-4	0.0	0.0	27.071476900932723	0.0
26	1.3460471977989435E-4	0.0	0.0	27.58467982921353	0.0
27	1.3460471977989435E-4	0.0	0.0	28.102952868606053	0.0
28	1.3460471977989435E-4	0.0	0.0	28.644198446841006	0.0
29	1.794729597065258E-4	0.0	0.0	29.227709907086847	0.0
30	1.794729597065258E-4	0.0	0.0	29.866633643642082	0.0
31	1.794729597065258E-4	0.0	0.0	30.438838307426412	0.0
32	1.794729597065258E-4	0.0	0.0	31.011671126569716	0.0
33	1.794729597065258E-4	0.0	0.0	31.569697426537232	0.0
34	1.794729597065258E-4	0.0	0.0	32.104706319422384	0.0
35	1.794729597065258E-4	0.0	0.0	32.668475754100506	0.0
36	1.794729597065258E-4	0.0	0.0	33.2003438701908	0.0
37	2.2434119963315728E-4	0.0	0.0	33.74953112689277	0.0
38	2.2434119963315728E-4	0.0	0.0	34.29014854976875	0.0
39	2.2434119963315728E-4	0.0	0.0	34.83969475239013	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	960	0.0	44.29688	1
CGTTTTT	31805	0.0	44.28549	1
CGTTTTC	790	0.0	40.44304	1
CGTTTCT	895	0.0	40.22346	1
GTTTTTT	34990	0.0	40.074306	2
AGCGTCG	40	3.4601726E-7	39.375	17
CGTTCTG	850	0.0	38.11765	1
TGGGCGA	3415	0.0	37.884335	6
TAGTGCG	30	1.14026574E-4	37.500004	1
CGTTTGG	1340	0.0	36.77239	2
TTACGGG	615	0.0	36.585365	3
TTGGGCG	2970	0.0	36.363636	5
CGTATGG	150	0.0	36.0	2
TCTTGCG	645	0.0	35.93023	1
TTTGGGC	7065	0.0	35.859875	4
TCGTTTG	865	0.0	35.63584	1
ACGGGTA	120	0.0	35.625004	5
TTGTACG	95	0.0	35.526314	1
TCTAGCG	95	0.0	35.526314	1
GGGCGAT	5355	0.0	35.462185	7
>>END_MODULE