##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933192.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1353465
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14759450743093	33.0	31.0	34.0	30.0	34.0
2	32.32680268791583	34.0	31.0	34.0	30.0	34.0
3	32.401800563738256	34.0	31.0	34.0	30.0	34.0
4	35.89560129002228	37.0	35.0	37.0	35.0	37.0
5	35.09414946082832	37.0	35.0	37.0	35.0	37.0
6	34.795651162017485	37.0	35.0	37.0	32.0	37.0
7	35.61427595098507	37.0	35.0	37.0	33.0	37.0
8	35.88604212151773	37.0	35.0	37.0	35.0	37.0
9	37.72919063293103	39.0	38.0	39.0	35.0	39.0
10	37.19376267579878	39.0	37.0	39.0	34.0	39.0
11	37.14020458600702	39.0	37.0	39.0	34.0	39.0
12	37.00286671616924	39.0	37.0	39.0	33.0	39.0
13	36.96032849020847	39.0	37.0	39.0	33.0	39.0
14	38.101345066181985	40.0	37.0	41.0	33.0	41.0
15	38.20374076906311	40.0	37.0	41.0	33.0	41.0
16	38.19688207674376	40.0	37.0	41.0	33.0	41.0
17	38.14928055029129	40.0	37.0	41.0	33.0	41.0
18	38.08468634209233	40.0	37.0	41.0	33.0	41.0
19	38.083148068106674	40.0	37.0	41.0	34.0	41.0
20	37.994958864839504	40.0	37.0	41.0	33.0	41.0
21	37.87633148991662	40.0	36.0	41.0	33.0	41.0
22	37.92089045523896	40.0	36.0	41.0	33.0	41.0
23	37.82527956023983	40.0	36.0	41.0	33.0	41.0
24	37.76179952935613	40.0	36.0	41.0	33.0	41.0
25	37.64024411418101	39.0	36.0	41.0	33.0	41.0
26	37.61349499248226	39.0	36.0	41.0	33.0	41.0
27	37.58547136423919	39.0	36.0	41.0	33.0	41.0
28	37.52220485937944	39.0	36.0	41.0	33.0	41.0
29	37.46262962100978	39.0	36.0	41.0	33.0	41.0
30	37.38507682134374	39.0	36.0	41.0	32.0	41.0
31	37.32221815857817	39.0	35.0	41.0	32.0	41.0
32	37.28979027902458	39.0	35.0	41.0	32.0	41.0
33	37.18569818946186	39.0	35.0	41.0	32.0	41.0
34	37.061683161367306	39.0	35.0	41.0	31.0	41.0
35	36.921596051615666	39.0	35.0	41.0	31.0	41.0
36	36.78606687280425	39.0	35.0	41.0	31.0	41.0
37	36.76382396293957	39.0	35.0	41.0	31.0	41.0
38	36.67578991699084	39.0	35.0	41.0	31.0	41.0
39	36.6128662359204	39.0	35.0	41.0	31.0	41.0
40	36.49535451600152	39.0	35.0	41.0	30.0	41.0
41	36.38400180278027	39.0	35.0	40.0	30.0	41.0
42	36.285655705910386	39.0	35.0	40.0	30.0	41.0
43	36.1422334526567	38.0	35.0	40.0	30.0	41.0
44	36.07032468515994	38.0	35.0	40.0	30.0	41.0
45	36.08167037935964	38.0	35.0	40.0	30.0	41.0
46	35.88097364911542	38.0	35.0	40.0	29.0	41.0
47	35.813732161526154	38.0	35.0	40.0	29.0	41.0
48	35.71652240730274	38.0	35.0	40.0	29.0	41.0
49	35.757609542913926	38.0	35.0	40.0	29.0	41.0
50	35.55260978303835	38.0	34.0	40.0	28.0	41.0
51	34.45337337869838	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	15.0
10	22.0
11	24.0
12	22.0
13	19.0
14	18.0
15	43.0
16	78.0
17	114.0
18	275.0
19	502.0
20	878.0
21	1533.0
22	2195.0
23	3314.0
24	4942.0
25	7469.0
26	10443.0
27	13208.0
28	15391.0
29	18640.0
30	23213.0
31	29860.0
32	38954.0
33	53449.0
34	86692.0
35	110277.0
36	120611.0
37	178108.0
38	285897.0
39	347127.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.896764969910564	4.408314954579542	54.473517970542275	16.22140210496762
2	20.417003764412083	4.483972618427518	53.12667856206108	21.972345055099318
3	21.24044581869498	4.481608316432268	51.99033591559442	22.287609949278334
4	19.282138806692455	5.098765021629669	50.92255802698998	24.696538144687892
5	17.46472941671931	8.16046222104007	49.43179173454799	24.94301662769263
6	22.141983723258452	7.675706427576627	53.32055132567152	16.861758523493403
7	83.93870547077317	2.225325368590987	9.249444943164397	4.586524217471453
8	85.83938262164149	2.816253098528591	7.160214708174943	4.184149571654975
9	79.30681620876786	5.445947992744548	9.740554798240073	5.5066810002475135
10	37.39549969892092	28.30239422519238	17.46857140746159	16.833534668425116
11	29.38169808602365	25.317167418440818	25.051478981724685	20.24965551381085
12	25.42045786185827	22.321670674897394	31.674701599228644	20.583169864015694
13	23.736705419054058	22.884079012017303	32.84717373556021	20.532041833368428
14	20.74519843512762	25.105414620991308	32.079292778165666	22.070094165715403
15	19.18874887788011	23.627873642835244	35.578607500009234	21.604769979275414
16	21.048198512706275	22.31805033746717	33.19487389773655	23.438877252090005
17	21.50170118917002	22.005149745283404	30.27318770710731	26.219961358439264
18	22.208996907936296	22.210696249995383	31.939281769384504	23.641025072683817
19	23.809333820970622	24.00675303757393	29.607932233194063	22.57598090826139
20	26.3993527723288	23.788572294074836	28.564019017854175	21.248055915742185
21	25.53047178907471	24.562290122020148	28.399256722560246	21.5079813663449
22	23.86504268673368	23.57009601282634	27.536877569793088	25.027983730646895
23	22.83634966548821	25.057611390024864	27.690483315046936	24.415555629439993
24	22.87196196429165	23.62196288784712	29.863720155305085	23.642354992556143
25	22.368070101554157	24.960601123782293	28.016978643703382	24.654350130960164
26	21.90038161311892	25.523231114214255	28.138740196458716	24.4376470762081
27	21.514039890207727	24.569456912443247	29.006069606528428	24.910433590820595
28	20.425352705832807	24.147650659603315	30.93467507471564	24.49232155984824
29	21.243622849501094	23.493847273479552	29.494150199672692	25.768379677346665
30	22.487097930127486	24.645631767352683	28.98168774220242	23.88558256031741
31	22.42895087793183	26.01138559179587	27.47451910466839	24.085144425603914
32	23.297462438999162	26.405854602815737	26.54712164703188	23.749561311153226
33	23.09649676940298	25.362532462974663	26.53766443905088	25.003306328571483
34	21.933703494364465	23.95680715792429	28.485258207637433	25.62423114007381
35	22.67653762749683	24.225524856571838	27.924179790389854	25.173757725541478
36	23.31460362846472	26.037466798180965	26.64716117520586	24.000768398148455
37	21.295341955647174	26.595885375683892	27.93903056229751	24.169742106371427
38	20.86104923289483	26.74712681894249	28.483558865578352	23.90826508258433
39	21.691288655414066	26.080689194031613	28.969570694476772	23.25845145607755
40	22.49818059573022	25.213064246212497	29.912040577332995	22.37671458072429
41	21.080707665140952	24.99118928084583	28.688440410354165	25.239662643659056
42	22.491826534117987	24.84814901013325	27.398639787508362	25.261384668240407
43	22.547609284318398	23.541502735571292	28.463092876431972	25.447795103678338
44	21.767020203699396	23.98214951993587	28.541040957837843	25.709789318526894
45	22.149889358055066	23.781183850339684	28.044759192147563	26.024167599457687
46	21.934590107612685	24.967915683080093	28.812935687291507	24.284558522015715
47	20.17192908571703	25.401617330333625	29.79308663319702	24.63336695075233
48	20.427273701203948	24.140853291366973	30.20388410487157	25.227988902557506
49	21.321423162032264	23.47596723964048	30.284048719397987	24.918560878929267
50	21.06364036011275	23.356348335568338	29.469842219784038	26.110169084534878
51	20.01499854078236	23.90730458489876	28.351896798217908	27.72580007610097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	298.0
1	1013.0
2	1728.0
3	4271.0
4	6814.0
5	4726.5
6	2639.0
7	2622.5
8	2606.0
9	2660.0
10	2714.0
11	2819.0
12	2924.0
13	3036.5
14	3149.0
15	3117.5
16	3086.0
17	3047.0
18	3008.0
19	3165.0
20	3322.0
21	3935.5
22	4549.0
23	4912.5
24	5276.0
25	6084.0
26	9102.5
27	11313.0
28	12903.0
29	14493.0
30	17772.0
31	21051.0
32	24478.0
33	27905.0
34	31622.0
35	35339.0
36	37871.5
37	40404.0
38	44480.5
39	48557.0
40	55479.0
41	62401.0
42	68262.5
43	74124.0
44	83296.5
45	92469.0
46	103258.5
47	114048.0
48	125579.0
49	137110.0
50	132320.0
51	127530.0
52	113862.0
53	100194.0
54	89769.0
55	79344.0
56	73964.0
57	68584.0
58	64512.0
59	60440.0
60	56719.5
61	52999.0
62	47347.5
63	41696.0
64	36194.0
65	30692.0
66	26392.5
67	22093.0
68	18807.5
69	15522.0
70	12913.5
71	10305.0
72	8285.0
73	6265.0
74	5184.0
75	3348.0
76	2593.0
77	1985.0
78	1377.0
79	1123.5
80	870.0
81	629.5
82	389.0
83	261.5
84	134.0
85	102.5
86	71.0
87	51.0
88	31.0
89	21.0
90	11.0
91	6.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1353465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.953549823700794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.71250557727579	24.192833192407065
2	10.092691064873401	6.449946135933041
3	3.623262419240113	3.4732828871259493
4	1.7755574895920427	2.269414588340978
5	1.014594061018401	1.6209940939791203
6	0.6800167964834988	1.3037370352433328
7	0.49420817252374377	1.1054193824812295
8	0.38066231700269404	0.9730809849880777
9	0.3315787786684069	0.953560712219652
>10	5.379043251112535	43.41033983431303
>50	0.4459088466612596	8.66715907604101
>100	0.06559802402977397	3.629765460974229
>500	0.002992190512562237	0.6800156233448079
>1k	0.0013810110057979556	1.2704509926085021
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	3717	0.27462845363566846	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3672	0.27130365395484923	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	3183	0.2351741640899469	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	3143	0.2322187865958854	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	2348	0.17348065890141232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2165331205461537E-4	0.0	0.0	0.29398617622177153	0.0
2	2.2165331205461537E-4	0.0	0.0	1.0071926499761723	0.0
3	2.2165331205461537E-4	0.0	0.0	1.4689703834232877	0.0
4	2.2165331205461537E-4	0.0	0.0	1.993328235307156	0.0
5	2.2165331205461537E-4	0.0	0.0	2.8637607917456305	0.0
6	2.2165331205461537E-4	0.0	0.0	3.566032368772004	0.0
7	2.2165331205461537E-4	0.0	0.0	4.185479491527302	0.0
8	2.2165331205461537E-4	0.0	0.0	5.235894537354125	0.0
9	2.2165331205461537E-4	0.0	0.0	5.848174869686324	0.0
10	2.2165331205461537E-4	0.0	0.0	6.850417262359943	0.0
11	2.2165331205461537E-4	0.0	0.0	8.450532522082211	0.0
12	2.2165331205461537E-4	0.0	0.0	9.896820383238577	0.0
13	2.2165331205461537E-4	0.0	0.0	10.360888534243589	0.0
14	2.2165331205461537E-4	0.0	0.0	10.52808901597012	0.0
15	2.2165331205461537E-4	0.0	0.0	10.768213437362622	0.0
16	2.2165331205461537E-4	0.0	0.0	11.409604238011326	0.0
17	2.2165331205461537E-4	0.0	0.0	12.29008507792961	0.0
18	2.2165331205461537E-4	0.0	0.0	13.236175298216061	0.0
19	2.2165331205461537E-4	0.0	0.0	13.839146191441966	1.4776887470307692E-4
20	2.2165331205461537E-4	0.0	0.0	14.504623318667273	1.4776887470307692E-4
21	2.2165331205461537E-4	0.0	0.0	15.39337921556893	1.4776887470307692E-4
22	2.9553774940615385E-4	0.0	0.0	16.31989005995722	1.4776887470307692E-4
23	2.9553774940615385E-4	0.0	0.0	17.22283176883037	1.4776887470307692E-4
24	2.9553774940615385E-4	0.0	0.0	17.92584219023026	1.4776887470307692E-4
25	2.9553774940615385E-4	0.0	0.0	18.535979873879267	1.4776887470307692E-4
26	2.9553774940615385E-4	0.0	0.0	19.072011466864677	1.4776887470307692E-4
27	2.9553774940615385E-4	0.0	0.0	19.603536109171646	1.4776887470307692E-4
28	2.9553774940615385E-4	0.0	0.0	20.147768874703075	1.4776887470307692E-4
29	2.9553774940615385E-4	0.0	0.0	20.768619801768054	1.4776887470307692E-4
30	2.9553774940615385E-4	0.0	0.0	21.44207644822733	1.4776887470307692E-4
31	2.9553774940615385E-4	0.0	0.0	22.0794036048217	1.4776887470307692E-4
32	2.9553774940615385E-4	0.0	0.0	22.684517146730798	1.4776887470307692E-4
33	2.9553774940615385E-4	0.0	0.0	23.298940127746192	1.4776887470307692E-4
34	2.9553774940615385E-4	0.0	0.0	23.904644745154105	1.4776887470307692E-4
35	2.9553774940615385E-4	0.0	0.0	24.54758711898719	1.4776887470307692E-4
36	2.9553774940615385E-4	0.0	0.0	25.145459986035842	1.4776887470307692E-4
37	2.9553774940615385E-4	0.0	0.0	25.739712515654265	1.4776887470307692E-4
38	2.9553774940615385E-4	0.0	0.0	26.325320566102558	1.4776887470307692E-4
39	3.694221867576923E-4	0.0	0.0	26.91321903410875	1.4776887470307692E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	30	2.1657888E-6	45.000004	31
GATCGAC	25	3.8911745E-5	45.0	9
TCGTTCA	235	0.0	42.12766	16
CTTCGAC	70	0.0	41.785717	21
TATCGCG	70	0.0	41.785717	32
CGTCTTA	40	3.4588265E-7	39.375004	42
TTTACGG	200	0.0	39.375	2
CGTTTGG	915	0.0	39.098362	2
GCGATAG	110	0.0	38.863636	9
TTCGGTA	70	0.0	38.57143	25
ACGTACC	35	6.249111E-6	38.57143	35
CGTTCAT	260	0.0	38.076927	17
TGCGACG	65	9.094947E-12	38.076927	1
GCGATCT	650	0.0	38.076923	9
CGTTTTT	2145	0.0	37.76224	1
TCGTTTG	555	0.0	37.7027	1
GTATGCG	30	1.140029E-4	37.500004	1
TTTGGGC	4025	0.0	37.118015	4
GGCGATA	225	0.0	37.0	8
TTGGGAC	2880	0.0	36.953125	5
>>END_MODULE