##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351318
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.130508542118534	33.0	31.0	34.0	30.0	34.0
2	32.3366266459447	34.0	31.0	34.0	30.0	34.0
3	32.38097393244866	34.0	31.0	34.0	30.0	34.0
4	35.893301794955	37.0	35.0	37.0	35.0	37.0
5	35.11281517030155	37.0	35.0	37.0	35.0	37.0
6	34.8250530858083	37.0	35.0	37.0	32.0	37.0
7	35.56923072543963	37.0	35.0	37.0	33.0	37.0
8	35.838306036126816	37.0	35.0	37.0	35.0	37.0
9	37.67477328232541	39.0	37.0	39.0	35.0	39.0
10	37.27653009524135	39.0	37.0	39.0	34.0	39.0
11	37.21510426451249	39.0	37.0	39.0	34.0	39.0
12	36.98731918091302	39.0	37.0	39.0	33.0	39.0
13	36.874970824153614	39.0	37.0	39.0	33.0	39.0
14	37.747357095281195	40.0	37.0	41.0	33.0	41.0
15	37.889780768420636	40.0	37.0	41.0	33.0	41.0
16	37.970340261529444	40.0	37.0	41.0	33.0	41.0
17	37.97619535577454	40.0	37.0	41.0	33.0	41.0
18	37.91330361666638	40.0	37.0	41.0	33.0	41.0
19	37.86145884924769	40.0	37.0	41.0	33.0	41.0
20	37.682321998872816	39.0	35.0	41.0	33.0	41.0
21	37.572404488241425	39.0	35.0	41.0	33.0	41.0
22	37.652642335433995	39.0	35.0	41.0	33.0	41.0
23	37.60399694863343	39.0	35.0	41.0	33.0	41.0
24	37.54120483436659	39.0	35.0	41.0	33.0	41.0
25	37.40231926630574	39.0	35.0	41.0	33.0	41.0
26	37.316027075185445	39.0	35.0	41.0	33.0	41.0
27	37.27279843332821	39.0	35.0	41.0	33.0	41.0
28	37.18236469523338	39.0	35.0	41.0	32.0	41.0
29	37.07316448345943	39.0	35.0	41.0	32.0	41.0
30	36.935366249380905	39.0	35.0	41.0	32.0	41.0
31	36.77502149050148	39.0	35.0	41.0	31.0	41.0
32	36.658952857525094	39.0	35.0	41.0	31.0	41.0
33	36.41079591708936	39.0	35.0	41.0	30.0	41.0
34	36.15524681342829	39.0	35.0	41.0	30.0	41.0
35	35.98360175112007	39.0	35.0	41.0	29.0	41.0
36	35.81703470929471	38.0	35.0	40.0	29.0	41.0
37	35.78310248834389	38.0	35.0	40.0	29.0	41.0
38	35.661864749315434	38.0	35.0	40.0	28.0	41.0
39	35.570195663188336	38.0	35.0	40.0	27.0	41.0
40	35.41700112149107	38.0	35.0	40.0	26.0	41.0
41	35.254615476576774	38.0	34.0	40.0	25.0	41.0
42	35.187576497646006	38.0	34.0	40.0	26.0	41.0
43	35.07799771147507	38.0	34.0	40.0	25.0	41.0
44	35.00315099140949	38.0	34.0	40.0	25.0	41.0
45	35.01588304612915	38.0	34.0	40.0	25.0	41.0
46	34.837685515686644	37.0	34.0	40.0	24.0	41.0
47	34.73946396142526	37.0	34.0	40.0	24.0	41.0
48	34.61982591270587	37.0	34.0	40.0	24.0	41.0
49	34.64734514030024	37.0	34.0	40.0	24.0	41.0
50	34.46230765289567	37.0	34.0	40.0	24.0	41.0
51	33.357832505023936	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	14.0
11	8.0
12	7.0
13	10.0
14	11.0
15	22.0
16	31.0
17	58.0
18	140.0
19	271.0
20	422.0
21	712.0
22	1014.0
23	1459.0
24	2256.0
25	3377.0
26	4629.0
27	5414.0
28	5592.0
29	5982.0
30	7002.0
31	8460.0
32	11208.0
33	15721.0
34	25520.0
35	27071.0
36	30902.0
37	44552.0
38	68984.0
39	80438.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.69192583357528	4.2172618539329045	52.51880063076757	19.57201168172425
2	24.952607039775927	4.209007224224207	49.19246950056644	21.645916235433425
3	21.07890856716707	4.125606999925993	51.977980063646044	22.817504369260895
4	18.736586226723368	4.799640212001663	51.83138922571573	24.632384335559237
5	17.393928008243247	7.647202819098367	51.06057759636569	23.898291576292703
6	21.218098702599924	7.200883530021235	53.9926789973756	17.588338770003244
7	77.65300952413483	2.2310271605781655	14.626065274196026	5.489898041090977
8	79.54872793309765	2.736552069634918	12.422933069185182	5.29178692808225
9	73.2464604717094	5.323097592494549	15.143545164210204	6.286896771585856
10	37.5952840446547	24.524220222134932	22.100205511815506	15.78029022139486
11	30.371344479929864	22.576412253286197	28.275237818728332	18.777005448055608
12	27.028219447907592	19.519922121838334	34.159934873818024	19.29192355643605
13	26.108539841397256	19.67761401351482	35.02923277486494	19.184613370222987
14	20.873396751660888	21.711383988295506	36.94715329132012	20.468065968723494
15	19.18176694618551	21.660717640428327	38.265332263077894	20.89218315030827
16	22.98402017545358	21.399415913787507	33.63647749332513	21.980086417433778
17	22.693400281226694	21.089155693702004	31.918375944301175	24.299068080770127
18	23.786142469215925	20.54634262975424	33.342726532657025	22.324788368372815
19	23.83794738669809	23.302250382844033	31.094905470257718	21.764896760200163
20	24.56520872827467	25.753590763923285	30.153308398658762	19.527892109143284
21	24.125720856887494	24.143653328323627	31.920083798723663	19.810542016065217
22	21.513842160094274	22.499558804274187	32.60948770060173	23.377111335029802
23	21.48936291337193	23.6034020460096	31.852054264227853	23.05518077639062
24	22.361507238456326	24.223637843776864	30.259480015256834	23.155374902509976
25	23.32160606629891	24.11518908794881	28.184721534336415	24.37848331141587
26	21.322847107179253	24.769297331762107	28.798979841624966	25.108875719433676
27	22.03388383174218	22.488457750528013	31.27195304538908	24.205705372340727
28	20.275078418982233	24.177241131965914	29.87378955817806	25.673890890873796
29	22.28009951098435	25.38668670549189	28.993105960981218	23.34010782254254
30	25.14957958316966	23.732629697311268	29.170438178516328	21.947352541002736
31	24.771574470992093	24.811424407516835	28.17219726857149	22.244803852919578
32	26.014323205756607	25.028891203980443	26.60495619353406	22.35182939672889
33	25.95511758577699	25.136201390193502	26.653914686978748	22.254766337050764
34	22.824335786950854	25.11769963394987	29.244445203490855	22.813519375608422
35	23.741453611827463	26.881059325169787	26.84547902470127	22.53200803830148
36	25.451869815950225	27.892109143283296	25.023198355905475	21.632822684861008
37	24.293090590291417	27.574732863104085	26.40627579571784	21.725900750886662
38	23.722382570776336	26.43360146647766	27.711645859306955	22.13237010343905
39	23.92647117426377	25.581382109655635	27.19046561804405	23.301681098036536
40	26.570514462680535	24.2486863753067	27.801877501295124	21.37892166071764
41	23.242475478056917	25.114853209912386	26.82242298999767	24.82024832203303
42	22.777654432736153	25.807957463039184	27.777967539380278	23.636420564844386
43	22.274691305313134	24.373075105744654	29.52168690474157	23.830546684200637
44	23.079375380709216	23.68822548232655	28.394502985898818	24.837896151065415
45	22.783347280811117	22.923960628262712	27.263334073403584	27.029358017522586
46	23.02301618476708	23.840793810735573	28.49441246961442	24.641777534882927
47	21.107657449945634	25.467525148156373	30.202266892103452	23.222550509794544
48	21.26079506316215	25.947716883279536	29.00619951155364	23.78528854200468
49	22.96694163122869	24.164432223797245	29.41978492419973	23.44884122077434
50	22.028760268474716	23.068558969366784	28.34753698928037	26.555143772878132
51	20.67557028105591	23.977706806938443	27.782806460243997	27.56391645176165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	173.0
2	297.0
3	2927.0
4	5557.0
5	3614.0
6	1671.0
7	1722.5
8	1774.0
9	1805.5
10	1837.0
11	1854.0
12	1871.0
13	1806.0
14	1741.0
15	1714.0
16	1687.0
17	1622.0
18	1557.0
19	1532.0
20	1507.0
21	1489.0
22	1471.0
23	1596.0
24	1721.0
25	1882.0
26	2302.0
27	2561.0
28	3001.0
29	3441.0
30	3832.5
31	4224.0
32	4985.5
33	5747.0
34	6540.0
35	7333.0
36	8040.5
37	8748.0
38	9176.5
39	9605.0
40	10554.5
41	11504.0
42	13221.0
43	14938.0
44	17339.0
45	19740.0
46	23720.5
47	27701.0
48	31855.5
49	36010.0
50	35294.0
51	34578.0
52	30399.5
53	26221.0
54	24761.0
55	23301.0
56	22231.0
57	21161.0
58	19729.5
59	18298.0
60	16273.5
61	14249.0
62	12562.5
63	10876.0
64	9702.5
65	8529.0
66	7272.0
67	6015.0
68	5031.5
69	4048.0
70	3483.5
71	2919.0
72	2397.5
73	1876.0
74	1610.0
75	1047.5
76	751.0
77	583.0
78	415.0
79	320.5
80	226.0
81	167.0
82	108.0
83	78.0
84	48.0
85	28.5
86	9.0
87	8.5
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	351318.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.32520632148764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69375284550127	38.03715615924084
2	10.494532626300666	10.982571698375319
3	5.942537712726606	9.328335356749228
4	3.989271473368254	8.349578116656756
5	2.5719968775134365	6.7290133637056275
6	1.5600753582850542	4.897875899960053
7	0.9514003793254524	3.4847554799782134
8	0.5602205687564019	2.3450925476575906
9	0.3007947606725474	1.4165233121352185
>10	0.8619827254926592	7.450577198911441
>50	0.04460476376835265	1.5714337916903112
>100	0.023934263485457234	2.2442954985470003
>500	0.0027198026688019586	0.9654597756590267
>1k	0.002175842135041567	2.197331800733398
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3493	0.9942559162923619	No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	1570	0.44688857388462877	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1335	0.3799976090038085	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1322	0.37629725775508227	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	860	0.24479246722342718	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC	757	0.21547429963736559	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	710	0.20209610666120154	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG	564	0.16053831571396854	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG	501	0.14260584427783377	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT	393	0.11186446467303128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.47819923829692756	0.0
2	0.0	0.0	0.0	1.5837503344548245	0.0
3	0.0	0.0	0.0	2.362531951109821	0.0
4	0.0	0.0	0.0	3.2668408678177605	0.0
5	0.0	0.0	0.0	4.644225459555161	0.0
6	0.0	0.0	0.0	5.798735049157743	0.0
7	0.0	0.0	0.0	6.797829886313824	0.0
8	2.8464240374817117E-4	0.0	0.0	8.134795256718984	0.0
9	2.8464240374817117E-4	0.0	0.0	9.081231249181654	0.0
10	2.8464240374817117E-4	0.0	0.0	10.404249141803152	0.0
11	2.8464240374817117E-4	0.0	0.0	12.28943578182729	0.0
12	2.8464240374817117E-4	0.0	0.0	13.761321651609084	0.0
13	2.8464240374817117E-4	0.0	0.0	14.368179256400184	0.0
14	2.8464240374817117E-4	0.0	0.0	14.668761634758253	0.0
15	2.8464240374817117E-4	0.0	0.0	14.984145418111227	0.0
16	2.8464240374817117E-4	0.0	0.0	15.579901969156149	0.0
17	2.8464240374817117E-4	0.0	0.0	16.413050284927046	0.0
18	2.8464240374817117E-4	0.0	0.0	17.362617343830944	0.0
19	2.8464240374817117E-4	0.0	0.0	17.934179290557275	0.0
20	2.8464240374817117E-4	0.0	0.0	18.51541907901104	0.0
21	2.8464240374817117E-4	0.0	0.0	19.13650880398955	0.0
22	2.8464240374817117E-4	0.0	0.0	19.7507671112781	0.0
23	2.8464240374817117E-4	0.0	0.0	20.34083081424806	0.0
24	2.8464240374817117E-4	0.0	0.0	20.804228647550083	0.0
25	2.8464240374817117E-4	0.0	0.0	21.197319807126306	0.0
26	2.8464240374817117E-4	0.0	0.0	21.576463488918872	0.0
27	2.8464240374817117E-4	0.0	0.0	22.02961419568596	0.0
28	2.8464240374817117E-4	0.0	0.0	22.441491753909563	0.0
29	2.8464240374817117E-4	0.0	0.0	22.895496387887896	0.0
30	2.8464240374817117E-4	0.0	0.0	23.444856227121868	0.0
31	5.692848074963423E-4	0.0	0.0	23.868688766302892	0.0
32	5.692848074963423E-4	0.0	0.0	24.279143112507757	0.0
33	5.692848074963423E-4	0.0	0.0	24.68959745871262	0.0
34	5.692848074963423E-4	0.0	0.0	25.13904781423098	0.0
35	5.692848074963423E-4	0.0	0.0	25.641157014442754	0.0
36	5.692848074963423E-4	0.0	0.0	26.076944534581205	0.0
37	5.692848074963423E-4	0.0	0.0	26.49337637126478	0.0
38	5.692848074963423E-4	0.0	0.0	26.973283463984195	0.0
39	5.692848074963423E-4	0.0	0.0	27.72587797949436	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTATG	20	7.0265913E-4	45.000004	1
TACCGGG	25	3.885258E-5	45.0	3
CGTTTTT	2080	0.0	43.70192	1
CGGGTAT	50	1.0768417E-9	40.5	6
CGTATGG	50	1.0768417E-9	40.5	2
TATGGGA	140	0.0	40.17857	4
GCGGGAT	90	0.0	40.000004	5
TAGGGCG	45	1.9217623E-8	40.000004	5
TTGGGAC	570	0.0	39.86842	5
GCAACGA	40	3.449095E-7	39.375004	9
CGCGCAA	40	3.449095E-7	39.375004	16
AACGACG	40	3.449095E-7	39.375004	11
TTACACG	160	0.0	39.375004	34
ACGCGCA	40	3.449095E-7	39.375004	15
TTTTGCG	315	0.0	39.285713	1
ACACGCG	155	0.0	39.193546	36
CGGGATT	100	0.0	38.25	6
CGGGATA	65	9.094947E-12	38.07692	6
GTTTTTT	2435	0.0	37.607803	2
TTTGGGA	1650	0.0	37.5	4
>>END_MODULE