FastQCFastQC Report
Sat 14 Jan 2017
SRR2933190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755883
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.3349881512606478No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT41180.23452587672413255Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC34950.19904515278068072Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC32910.1874270666097912Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC31890.18161802352434644Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC207.0343056E-445.038
CGTAGCG501.0822987E-940.52
CGTTTTT38800.040.128871
TACGCGG659.094947E-1238.076922
CGCTATA301.14016744E-437.49999623
TTTGGGA116700.037.1336754
TTGGGAC49450.036.946415
TGGGCGA32400.036.8756
TTGGGAT71400.036.74375
TTTGGGC44950.036.740824
CACGACC1850.036.48648527
TACGAAT1850.036.48648512
TTGGGCG24750.036.3636365
TTCACGG1800.036.2500042
CGTTTGG12200.036.147542
GCGATCT6300.036.0714269
TACCGGG1250.036.0000043
CTACGAA1750.036.011
GTATCGC250.00210741136.033
CGACTTA250.00210741136.023
Produced by FastQC (version 0.11.5)