##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933190.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1755883
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25910325460182	33.0	31.0	34.0	30.0	34.0
2	32.439329955355795	34.0	31.0	34.0	31.0	34.0
3	32.50444534174544	34.0	31.0	34.0	30.0	34.0
4	35.982951597572274	37.0	35.0	37.0	35.0	37.0
5	35.18483008264218	37.0	35.0	37.0	35.0	37.0
6	34.905071123759384	37.0	35.0	37.0	32.0	37.0
7	35.71731203047128	37.0	35.0	37.0	35.0	37.0
8	36.01310565681199	37.0	35.0	37.0	35.0	37.0
9	37.88626804861144	39.0	38.0	39.0	35.0	39.0
10	37.352266637355676	39.0	37.0	39.0	34.0	39.0
11	37.18702726776215	39.0	37.0	39.0	34.0	39.0
12	37.13401405446718	39.0	37.0	39.0	34.0	39.0
13	37.15889498332178	39.0	37.0	39.0	33.0	39.0
14	38.35418077400374	40.0	38.0	41.0	34.0	41.0
15	38.44230965275021	40.0	38.0	41.0	34.0	41.0
16	38.41187368406665	40.0	38.0	41.0	34.0	41.0
17	38.36768224306517	40.0	38.0	41.0	34.0	41.0
18	38.31535358563185	40.0	38.0	41.0	34.0	41.0
19	38.326686914788745	40.0	37.0	41.0	34.0	41.0
20	38.242422188722145	40.0	37.0	41.0	34.0	41.0
21	38.13675056937165	40.0	37.0	41.0	34.0	41.0
22	38.18196314902531	40.0	37.0	41.0	34.0	41.0
23	38.10257574109437	40.0	37.0	41.0	34.0	41.0
24	38.07445313839248	40.0	37.0	41.0	34.0	41.0
25	37.97840801465701	40.0	37.0	41.0	33.0	41.0
26	37.953581189635074	40.0	37.0	41.0	33.0	41.0
27	37.91697510597232	40.0	37.0	41.0	33.0	41.0
28	37.83889188516547	40.0	36.0	41.0	33.0	41.0
29	37.80387132855663	40.0	36.0	41.0	33.0	41.0
30	37.72665604712842	40.0	36.0	41.0	33.0	41.0
31	37.66092330753245	40.0	36.0	41.0	33.0	41.0
32	37.62531956855895	40.0	36.0	41.0	33.0	41.0
33	37.50102484049336	40.0	36.0	41.0	33.0	41.0
34	37.40975053577032	40.0	36.0	41.0	33.0	41.0
35	37.29946243570898	40.0	36.0	41.0	32.0	41.0
36	37.17401444173672	40.0	36.0	41.0	32.0	41.0
37	37.146107115337415	40.0	36.0	41.0	32.0	41.0
38	37.0772232546246	39.0	35.0	41.0	31.0	41.0
39	37.03385191382341	39.0	35.0	41.0	31.0	41.0
40	36.979022520293206	39.0	35.0	41.0	31.0	41.0
41	36.88280938991949	39.0	35.0	41.0	31.0	41.0
42	36.79965692474954	39.0	35.0	41.0	31.0	41.0
43	36.66932477847328	39.0	35.0	41.0	31.0	41.0
44	36.62115300393022	39.0	35.0	41.0	31.0	41.0
45	36.63574850943941	39.0	35.0	41.0	31.0	41.0
46	36.459318758710005	39.0	35.0	41.0	30.0	41.0
47	36.3913774437135	39.0	35.0	40.0	30.0	41.0
48	36.296605183830586	39.0	35.0	40.0	30.0	41.0
49	36.34572007360399	39.0	35.0	40.0	30.0	41.0
50	36.16983022217312	39.0	35.0	40.0	30.0	41.0
51	35.17825390416104	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	22.0
10	27.0
11	28.0
12	25.0
13	22.0
14	18.0
15	41.0
16	55.0
17	127.0
18	247.0
19	473.0
20	891.0
21	1546.0
22	2557.0
23	3886.0
24	5875.0
25	9127.0
26	12816.0
27	15290.0
28	17297.0
29	20157.0
30	24763.0
31	32463.0
32	42838.0
33	59853.0
34	99152.0
35	127201.0
36	141579.0
37	214915.0
38	378484.0
39	543874.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.405612446842984	4.2539850320323165	55.35471326961991	15.985689251504798
2	20.12577147794016	4.144638338659239	54.32246909389748	21.407121089503118
3	20.280793196357617	4.528262987909787	54.0713134075562	21.1196304081764
4	17.72265008545558	4.587948058042592	53.08753487561528	24.601866980886538
5	16.855451075043153	7.624312098243448	52.06856037674492	23.451676449968478
6	20.91887671331176	7.361424422925674	54.64601001319564	17.073688850566924
7	85.0284443781277	1.6257916956881522	9.292247832002474	4.053516094181673
8	88.27068773944505	1.9218820388374396	6.389605685572445	3.417824536145062
9	82.50367478926557	5.1489763270103985	8.156409054589627	4.190939829134401
10	39.09941607726711	32.539981308549606	15.012560631887203	13.348041982296088
11	25.2021347663825	26.296740728169244	29.31624715314175	19.184877352306504
12	22.280527802820576	23.928872254016927	33.589538710722756	20.201061232439745
13	21.804471026828097	24.792369423247447	34.486751110409976	18.91640843951448
14	20.410300686321357	26.55324984637359	33.04206487562099	19.99438459168407
15	19.067272705527646	24.9476189472761	35.69207059923696	20.29303774795929
16	20.943935330543095	25.39554173028613	32.046953014523176	21.6135699246476
17	21.308822968272942	23.72105658520528	31.578812483519688	23.39130796300209
18	22.326658439087343	23.035646452525597	33.05601796930661	21.58167713908045
19	22.872822391924746	25.25037260455281	30.95627669952952	20.92052830399292
20	24.543605695823697	26.372770850905212	29.57566079288882	19.50796266038227
21	24.0159509488958	26.345889788784334	30.0516036660757	19.586555596244168
22	22.940252852838146	24.419679443334207	29.67407281692459	22.965994886903058
23	20.66977127747122	26.10919975875386	29.49291040462263	23.72811855915229
24	20.548009178288076	25.303280457752596	31.523797428416355	22.62491293554297
25	20.298448131225143	27.128345111832626	29.66433412704605	22.90887262989618
26	20.417419611671164	27.314234490566854	29.42980825032192	22.838537647440063
27	20.009932324648055	26.268321978172803	30.998933300225584	22.722812396953557
28	19.437058163898165	25.63957849127761	32.17885246340445	22.744510881419775
29	20.629905295512284	24.375656008970985	31.299693658404347	23.694745037112384
30	21.662718985262686	25.460067669656805	31.321164337259372	21.556049007821137
31	22.83267165295182	27.907326399310207	28.380307799551563	20.879694148186413
32	22.682832512189023	27.385651549676144	28.050559177348376	21.880956760786454
33	21.85082946870606	27.649792155855486	27.836592757034495	22.662785618403962
34	21.32869900784961	26.482744009709076	28.717517055521352	23.47103992691996
35	20.8052586647288	26.793812571794362	29.319037771878875	23.08189099159796
36	21.373861470268803	28.46943674493118	28.52428094582612	21.632420838973896
37	21.640337083962883	29.147044535427476	28.213667994963217	20.998950385646424
38	22.59279234436463	28.102954467922974	27.748887596724835	21.555365590987556
39	21.54551299830342	27.378931284145924	27.805098631286935	23.270457086263722
40	21.834028804880507	26.847631647438924	28.67303800993574	22.645301537744828
41	20.675352514945473	27.666706722486634	27.97293441533405	23.68500634723384
42	21.748487797877196	27.633162346238333	27.941611143794887	22.676738712089588
43	21.515271803417427	26.65747091349481	29.252632436215855	22.574624846871917
44	21.855556435138332	25.715096051388393	28.78830764919986	23.641039864273417
45	22.06041063100446	25.53404754189203	27.781862458945156	24.623679368158356
46	22.373244686576495	25.96129696568621	28.835748167730994	22.8297101800063
47	20.61031401294961	26.932375334803062	29.75158367613332	22.70572697611401
48	21.112283677215395	26.476707161012435	29.31972118871246	23.091287973059707
49	22.243908050821155	24.986061144165074	30.15075605834785	22.619274746665923
50	21.784310230237438	25.576874996796484	28.7942305950909	23.844584177875177
51	20.831627164224496	24.72009809309618	28.138435191866428	26.309839550812896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	228.0
1	729.0
2	1230.0
3	5728.0
4	10226.0
5	6912.5
6	3599.0
7	3571.0
8	3543.0
9	3728.5
10	3914.0
11	4003.5
12	4093.0
13	4164.5
14	4236.0
15	4189.5
16	4143.0
17	4555.0
18	4967.0
19	5079.5
20	5192.0
21	6017.5
22	6843.0
23	7705.5
24	8568.0
25	10981.5
26	16366.0
27	19337.0
28	22916.0
29	26495.0
30	30839.5
31	35184.0
32	40315.5
33	45447.0
34	52262.5
35	59078.0
36	65743.0
37	72408.0
38	75783.0
39	79158.0
40	86398.5
41	93639.0
42	103019.0
43	112399.0
44	122584.5
45	132770.0
46	147739.5
47	162709.0
48	173664.5
49	184620.0
50	176431.5
51	168243.0
52	147035.5
53	125828.0
54	110877.5
55	95927.0
56	84525.0
57	73123.0
58	65171.0
59	57219.0
60	50982.5
61	44746.0
62	38170.0
63	31594.0
64	26879.5
65	22165.0
66	18084.5
67	14004.0
68	11722.5
69	9441.0
70	7786.5
71	6132.0
72	5005.5
73	3879.0
74	3264.5
75	2331.0
76	2012.0
77	1392.5
78	773.0
79	597.0
80	421.0
81	279.5
82	138.0
83	104.0
84	70.0
85	57.0
86	44.0
87	28.0
88	12.0
89	24.0
90	36.0
91	20.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1755883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.55114983709088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66079973879104	23.871852293527223
2	10.49360325764113	6.621704974256406
3	3.571654233406064	3.3806939365342394
4	1.6866673076777656	2.1286517179945537
5	0.97875609888086	1.5440440164878273
6	0.6530760893694937	1.2363180930430948
7	0.4651943379265928	1.0274191381501752
8	0.4015339373068778	1.0135085936517083
9	0.3272660376333516	0.929305781096481
>10	5.0704078721713515	40.952510988930456
>50	0.6210623158733137	11.95034187967746
>100	0.06637162930546715	3.526906877883329
>500	0.002164286410162228	0.457685070588677
>1k	0.0012625004059279665	1.024342777031801
>5k	1.8035720084685235E-4	0.33471386114666146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5882	0.3349881512606478	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	4118	0.23452587672413255	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	3495	0.19904515278068072	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	3291	0.1874270666097912	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	3189	0.18161802352434644	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.695140279847803E-5	0.0	0.0	0.24876372742375205	0.0
2	5.695140279847803E-5	0.0	0.0	0.8734636647202576	0.0
3	5.695140279847803E-5	0.0	0.0	1.305041395127124	0.0
4	5.695140279847803E-5	0.0	0.0	1.7987531059871302	0.0
5	5.695140279847803E-5	0.0	0.0	2.61674610438167	0.0
6	5.695140279847803E-5	0.0	0.0	3.3146855456770186	0.0
7	5.695140279847803E-5	0.0	0.0	3.9216166453003987	0.0
8	5.695140279847803E-5	0.0	0.0	4.927663175735513	0.0
9	5.695140279847803E-5	0.0	0.0	5.493646216746788	0.0
10	5.695140279847803E-5	0.0	0.0	6.3800378499023	0.0
11	5.695140279847803E-5	0.0	0.0	7.937146153815488	0.0
12	5.695140279847803E-5	0.0	0.0	9.386559355036754	0.0
13	5.695140279847803E-5	0.0	0.0	9.870987987240607	0.0
14	5.695140279847803E-5	0.0	0.0	10.048106849943874	0.0
15	5.695140279847803E-5	0.0	0.0	10.256435081380706	0.0
16	5.695140279847803E-5	0.0	0.0	10.741319324806948	0.0
17	5.695140279847803E-5	0.0	0.0	11.520585369298525	0.0
18	5.695140279847803E-5	0.0	0.0	12.365744186827937	0.0
19	5.695140279847803E-5	0.0	0.0	12.997221341057463	0.0
20	5.695140279847803E-5	0.0	0.0	13.607113913626364	0.0
21	5.695140279847803E-5	0.0	0.0	14.440711596387686	0.0
22	5.695140279847803E-5	0.0	0.0	15.358369549679564	0.0
23	5.695140279847803E-5	0.0	0.0	16.295220125714525	0.0
24	5.695140279847803E-5	0.0	0.0	16.99196358755111	0.0
25	5.695140279847803E-5	0.0	0.0	17.595591505812177	0.0
26	5.695140279847803E-5	0.0	0.0	18.121252953642127	0.0
27	5.695140279847803E-5	0.0	0.0	18.64315560888738	0.0
28	5.695140279847803E-5	0.0	0.0	19.201165453506867	0.0
29	5.695140279847803E-5	0.0	0.0	19.789929055637533	0.0
30	5.695140279847803E-5	0.0	0.0	20.498233652242206	0.0
31	1.1390280559695605E-4	0.0	0.0	21.120883339037967	0.0
32	1.1390280559695605E-4	0.0	0.0	21.67148950129365	0.0
33	1.1390280559695605E-4	0.0	0.0	22.279274871959007	0.0
34	1.1390280559695605E-4	0.0	0.0	22.868038474089676	0.0
35	1.1390280559695605E-4	0.0	0.0	23.521669724007808	0.0
36	1.1390280559695605E-4	0.0	0.0	24.103314400788662	0.0
37	1.708542083954341E-4	0.0	0.0	24.66508303799285	0.0
38	2.8475701399239016E-4	0.0	0.0	25.24735418020449	0.0
39	2.8475701399239016E-4	0.0	0.0	25.871370700667413	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGC	20	7.0343056E-4	45.0	38
CGTAGCG	50	1.0822987E-9	40.5	2
CGTTTTT	3880	0.0	40.12887	1
TACGCGG	65	9.094947E-12	38.07692	2
CGCTATA	30	1.14016744E-4	37.499996	23
TTTGGGA	11670	0.0	37.133675	4
TTGGGAC	4945	0.0	36.94641	5
TGGGCGA	3240	0.0	36.875	6
TTGGGAT	7140	0.0	36.7437	5
TTTGGGC	4495	0.0	36.74082	4
CACGACC	185	0.0	36.486485	27
TACGAAT	185	0.0	36.486485	12
TTGGGCG	2475	0.0	36.363636	5
TTCACGG	180	0.0	36.250004	2
CGTTTGG	1220	0.0	36.14754	2
GCGATCT	630	0.0	36.071426	9
TACCGGG	125	0.0	36.000004	3
CTACGAA	175	0.0	36.0	11
GTATCGC	25	0.002107411	36.0	33
CGACTTA	25	0.002107411	36.0	23
>>END_MODULE