##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933189.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833532
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17901412303307	33.0	31.0	34.0	30.0	34.0
2	32.3718729454898	34.0	31.0	34.0	30.0	34.0
3	32.43391135553284	34.0	31.0	34.0	30.0	34.0
4	35.9233550721508	37.0	35.0	37.0	35.0	37.0
5	35.23326398986482	37.0	35.0	37.0	35.0	37.0
6	34.91521861188293	37.0	35.0	37.0	32.0	37.0
7	35.680550956651935	37.0	35.0	37.0	35.0	37.0
8	35.954812772635	37.0	35.0	37.0	35.0	37.0
9	37.82360245317516	39.0	38.0	39.0	35.0	39.0
10	37.25069223497118	39.0	37.0	39.0	34.0	39.0
11	37.14400286971586	39.0	37.0	39.0	34.0	39.0
12	37.00097176833043	39.0	37.0	39.0	33.0	39.0
13	36.95332152814769	39.0	37.0	39.0	33.0	39.0
14	38.094880580469614	40.0	37.0	41.0	33.0	41.0
15	38.174879908629784	40.0	37.0	41.0	33.0	41.0
16	38.16902530436744	40.0	37.0	41.0	33.0	41.0
17	38.12319623001876	40.0	37.0	41.0	33.0	41.0
18	38.08979859201566	40.0	37.0	41.0	33.0	41.0
19	38.10440391010783	40.0	37.0	41.0	34.0	41.0
20	38.02190677742426	40.0	37.0	41.0	34.0	41.0
21	37.89712452551312	40.0	36.0	41.0	33.0	41.0
22	37.940777318687225	40.0	36.0	41.0	33.0	41.0
23	37.86834338693655	40.0	36.0	41.0	33.0	41.0
24	37.806830451620336	40.0	36.0	41.0	33.0	41.0
25	37.71478599501879	40.0	36.0	41.0	33.0	41.0
26	37.67895413733366	40.0	36.0	41.0	33.0	41.0
27	37.61138504580508	39.0	36.0	41.0	33.0	41.0
28	37.55057754231391	39.0	36.0	41.0	33.0	41.0
29	37.494225776574865	39.0	36.0	41.0	33.0	41.0
30	37.40887692374138	39.0	36.0	41.0	32.0	41.0
31	37.344604646252336	39.0	35.0	41.0	32.0	41.0
32	37.31384397959526	39.0	35.0	41.0	32.0	41.0
33	37.198994159792306	39.0	35.0	41.0	32.0	41.0
34	37.09608389359977	39.0	35.0	41.0	31.0	41.0
35	36.99241660788068	39.0	35.0	41.0	31.0	41.0
36	36.87110512853736	39.0	35.0	41.0	31.0	41.0
37	36.82057917392493	39.0	35.0	41.0	31.0	41.0
38	36.72246896339912	39.0	35.0	41.0	31.0	41.0
39	36.70221179270862	39.0	35.0	41.0	31.0	41.0
40	36.62169178867758	39.0	35.0	41.0	31.0	41.0
41	36.52638770917013	39.0	35.0	41.0	30.0	41.0
42	36.424833119784246	39.0	35.0	40.0	30.0	41.0
43	36.258791504105425	39.0	35.0	40.0	30.0	41.0
44	36.175947654079266	39.0	35.0	40.0	30.0	41.0
45	36.17131195922892	38.0	35.0	40.0	30.0	41.0
46	35.91956157652016	38.0	35.0	40.0	29.0	41.0
47	35.84857330012525	38.0	35.0	40.0	29.0	41.0
48	35.777310289227046	38.0	35.0	40.0	29.0	41.0
49	35.819185106270666	38.0	35.0	40.0	29.0	41.0
50	35.60565041294156	38.0	34.0	40.0	28.0	41.0
51	34.45089090760763	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	12.0
11	16.0
12	8.0
13	13.0
14	10.0
15	19.0
16	32.0
17	76.0
18	143.0
19	307.0
20	539.0
21	879.0
22	1326.0
23	2035.0
24	3175.0
25	4657.0
26	6629.0
27	7916.0
28	9159.0
29	11135.0
30	13913.0
31	17954.0
32	23140.0
33	31941.0
34	52247.0
35	68644.0
36	71776.0
37	106931.0
38	178252.0
39	220548.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.518751529635335	4.107340809950908	56.516246526828006	16.85766113358575
2	19.962520935009092	4.3168108722880465	54.1286957189406	21.591972473762254
3	19.60488619513108	4.523761535249996	54.397191709496454	21.474160560122467
4	18.034700527394268	4.803654808693607	52.89215051131811	24.26949415259402
5	17.02166203577067	7.763229246147718	51.385669656353926	23.829439061727683
6	21.01467010264753	6.968178786177376	54.68464318106563	17.33250793010946
7	83.80290138830902	1.8559575397225303	9.949947932412913	4.39119313955553
8	87.0892779161448	2.1681231194483233	6.743352384791465	3.9992465796154195
9	81.24079219514067	4.9518194862344815	8.872004914028496	4.935383404596344
10	37.554646972161834	31.183445866505426	16.140352140049814	15.121555021282926
11	28.425303407667613	25.6704001765979	26.158443826991647	19.745852588742842
12	24.099014794872904	23.589136349894186	32.27962453751026	20.032224317722655
13	22.51455253067669	25.51047830197281	32.46246094930969	19.512508218040818
14	20.629441941041254	27.284855290498744	31.74383227038674	20.34187049807326
15	18.83071075855516	24.988242802915785	35.40224010595874	20.778806332570316
16	21.029546556101025	24.583219360504454	31.551998003675923	22.835236079718594
17	20.744614483907036	23.753737109073196	29.673245898177875	25.82840250884189
18	22.074617411209168	22.73482001890749	31.989533695167072	23.20102887471627
19	22.866308672012593	25.438375491282876	29.618298997518995	22.077016839185536
20	25.594338309746956	25.072222782088748	29.14609157176929	20.187347336395003
21	24.72106649774694	25.91682142977114	28.42722295004871	20.934889122433212
22	22.95568736413239	24.699951531554877	27.93534021489277	24.409020889419963
23	21.615726810728322	25.938416281558474	27.427621255092788	25.01823565262041
24	21.13056247390622	24.753818689624392	30.31449302486287	23.801125811606514
25	21.027027156725836	26.47408857728318	28.573468085208486	23.925416180782502
26	21.011430874879427	27.54567311153021	27.680281021004593	23.762614992585767
27	20.7443745411094	27.448856192683664	28.517561413359054	23.28920785284788
28	19.330991491628396	25.87711089676221	31.494291760844217	23.297605850765176
29	20.91485389883052	24.81932307337931	30.105022962525734	24.16080006526444
30	21.25473287168339	26.560467984432513	29.83460742958879	22.350191714295313
31	22.440650149004476	28.00816285397561	27.150007438226726	22.40117955879318
32	23.104691841464994	27.757662573242538	26.422380904392394	22.715264680900074
33	21.63792151950975	26.553749586098675	27.13165181420749	24.676677080184085
34	20.31187764836863	25.87951032473858	28.462974426896626	25.345637599996163
35	21.40337743481954	26.332642298076138	27.203634653498604	25.060345613605715
36	22.96204584826977	27.068906772625407	27.33968221975881	22.629365159346012
37	20.396697427333326	28.140371335473624	28.66740569048339	22.795525546709662
38	19.70650196993037	28.837165219811595	28.624935815301633	22.831396994956403
39	20.512469827193197	26.74318442483312	28.66716574768575	24.07718000028793
40	22.663916922205747	24.43673428254704	28.61329858961624	24.28605020563098
41	21.598450929298455	24.57794061895644	26.559988098837238	27.263620352907868
42	23.141163146705825	24.771334513851897	26.079862560765516	26.00763977867676
43	21.420653316249407	25.08505972176233	28.26166241967915	25.23262454230911
44	20.91053492847305	25.385828018600364	28.992288238483944	24.711348814442637
45	21.507512608994016	25.37970947726062	26.95073494478916	26.1620429689562
46	22.114447915616918	25.697633684129706	28.25806327771459	23.929855122538786
47	19.967319790961835	26.15472471362827	29.323529270621883	24.554426224788013
48	20.50431177207354	25.05950581381399	29.32712841258644	25.109054001526037
49	20.685948469884778	23.906460699769173	31.080030520723863	24.327560309622186
50	21.300082060436793	24.242380616461034	29.09750315524779	25.360034167854383
51	20.430169447603692	23.204388073883187	27.799532591430204	28.565909887082917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	424.0
2	749.0
3	2315.0
4	3881.0
5	2625.5
6	1370.0
7	1415.0
8	1460.0
9	1571.5
10	1683.0
11	1822.5
12	1962.0
13	2022.0
14	2082.0
15	2046.0
16	2010.0
17	1961.5
18	1913.0
19	2087.5
20	2262.0
21	2560.5
22	2859.0
23	3416.5
24	3974.0
25	4374.5
26	5700.0
27	6625.0
28	7846.0
29	9067.0
30	10844.0
31	12621.0
32	14610.0
33	16599.0
34	19583.0
35	22567.0
36	24469.0
37	26371.0
38	29580.5
39	32790.0
40	37655.0
41	42520.0
42	47957.5
43	53395.0
44	58726.0
45	64057.0
46	72365.0
47	80673.0
48	87432.5
49	94192.0
50	90028.0
51	85864.0
52	73562.5
53	61261.0
54	54396.5
55	47532.0
56	41857.5
57	36183.0
58	32910.5
59	29638.0
60	27069.5
61	24501.0
62	21524.5
63	18548.0
64	16215.0
65	13882.0
66	11158.5
67	8435.0
68	6636.0
69	4837.0
70	4321.5
71	3806.0
72	3311.0
73	2816.0
74	2226.0
75	1220.0
76	804.0
77	681.5
78	559.0
79	444.0
80	329.0
81	255.5
82	182.0
83	143.0
84	104.0
85	65.5
86	27.0
87	26.0
88	25.0
89	14.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	833532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.534547044898474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.01276656291591	27.01079229442821
2	10.499716937251383	7.462053709297532
3	3.662240920943294	3.9040821688503535
4	1.6407117157562927	2.332077906026323
5	0.9775244821248207	1.736794484880223
6	0.6590897915837043	1.4052274323506082
7	0.47739313673322675	1.1874764213310947
8	0.396425558655511	1.1269442131075533
9	0.3257889277016525	1.0419085780309258
>10	5.057950843310809	42.63251569723956
>50	0.2333737014990357	5.176538984664266
>100	0.05337095851985829	3.0003775437764837
>500	0.001988979820615522	0.4877925251767553
>1k	0.0016574831838462678	1.4954180408401132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	3355	0.4025040430361402	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	2532	0.30376758180849683	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT	2523	0.3026878392191302	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2219	0.2662165339782996	No Hit
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	2066	0.24786090995906573	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG	968	0.11613231405632897	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.59914196455565E-4	0.0	0.0	0.3263222047863789	0.0
2	3.59914196455565E-4	0.0	0.0	1.3848298565621955	0.0
3	3.59914196455565E-4	0.0	0.0	1.9898456208040003	0.0
4	3.59914196455565E-4	0.0	0.0	2.6409304021921174	0.0
5	3.59914196455565E-4	0.0	0.0	3.69895816837266	0.0
6	3.59914196455565E-4	0.0	0.0	4.444940326226227	0.0
7	3.59914196455565E-4	0.0	0.0	5.142334067558294	0.0
8	3.59914196455565E-4	0.0	0.0	6.182006209719603	0.0
9	3.59914196455565E-4	0.0	0.0	6.740832985416277	0.0
10	3.59914196455565E-4	0.0	0.0	7.731316854061991	0.0
11	3.59914196455565E-4	0.0	0.0	9.50797329916548	0.0
12	3.59914196455565E-4	0.0	0.0	11.093035420355788	0.0
13	4.7988559527408663E-4	0.0	0.0	11.607832692686063	0.0
14	4.7988559527408663E-4	0.0	0.0	11.781071392580008	0.0
15	4.7988559527408663E-4	0.0	0.0	12.046208183968941	0.0
16	5.998569940926083E-4	0.0	0.0	12.728125614853418	0.0
17	5.998569940926083E-4	0.0	0.0	13.794191464754803	0.0
18	5.998569940926083E-4	0.0	0.0	14.923122327637092	0.0
19	5.998569940926083E-4	0.0	0.0	15.653268260846614	0.0
20	5.998569940926083E-4	0.0	0.0	16.382574394264406	0.0
21	5.998569940926083E-4	0.0	0.0	17.36585997898101	0.0
22	5.998569940926083E-4	0.0	0.0	18.434445228257584	0.0
23	5.998569940926083E-4	0.0	0.0	19.468118800477967	0.0
24	5.998569940926083E-4	0.0	0.0	20.298560823099773	0.0
25	5.998569940926083E-4	0.0	0.0	20.984077395948805	0.0
26	5.998569940926083E-4	0.0	0.0	21.590292874178797	0.0
27	5.998569940926083E-4	0.0	0.0	22.211864691457556	0.0
28	5.998569940926083E-4	0.0	0.0	22.844593848826438	0.0
29	5.998569940926083E-4	0.0	0.0	23.548705988492344	0.0
30	5.998569940926083E-4	0.0	0.0	24.34495616245087	0.0
31	5.998569940926083E-4	0.0	0.0	25.048348473723863	0.0
32	5.998569940926083E-4	0.0	0.0	25.689355657611227	0.0
33	5.998569940926083E-4	0.0	0.0	26.339480667808793	0.0
34	5.998569940926083E-4	0.0	0.0	26.974729224552867	0.0
35	5.998569940926083E-4	0.0	0.0	27.687119390737248	0.0
36	5.998569940926083E-4	0.0	0.0	28.331125859595073	0.0
37	5.998569940926083E-4	0.0	0.0	28.957976418421847	0.0
38	5.998569940926083E-4	0.0	0.0	29.610140942399333	0.0
39	5.998569940926083E-4	0.0	0.0	30.27490246325276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	30	2.164781E-6	45.000004	2
ACGTTGT	20	7.03217E-4	45.0	30
CGATTAC	20	7.03217E-4	45.0	10
CGTTAGA	20	7.03217E-4	45.0	22
CTACGGG	80	0.0	45.0	3
TGCGACG	35	1.2113924E-7	45.0	28
ACTACGT	20	7.03217E-4	45.0	34
TTGCGAC	40	6.8102963E-9	45.0	27
TATACGA	25	3.8898805E-5	45.0	24
AGGCACG	85	0.0	42.35294	10
TGTACGG	65	0.0	41.538464	2
CGATTCA	55	6.002665E-11	40.909092	20
TACGGGA	220	0.0	40.909092	4
TCGTACA	55	6.002665E-11	40.909092	34
CGTTTTT	1445	0.0	40.32872	1
TTACCGG	45	1.9272193E-8	40.0	2
ACGGGAT	155	0.0	39.19355	5
TTCGGGC	365	0.0	38.835613	4
GGACCGA	495	0.0	38.636364	8
TACGGAC	35	6.246226E-6	38.571426	19
>>END_MODULE