FastQCFastQC Report
Sat 14 Jan 2017
SRR2933188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816890
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152640.8401169030596239No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT31430.17298790790856905Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC24080.13253416552460523No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC23870.13137834431363485No Hit
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC22420.12339767404741069No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC22380.12317751762627348No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG19820.10908750667349151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76400.042.0255241
TCGTTTG6300.038.2142871
TCGTACA950.037.89473734
TTTGGGA91350.037.4876864
TTTGGGC47350.037.0644154
ACATACG3100.037.0161317
CACGACC5850.036.92307727
TTACACG5450.036.74312234
TGGGCGA32600.036.7177936
GCTACGA2100.036.4285710
TACACGC5500.036.40909235
CGTTATT2600.036.3461571
TTGGGAC37950.036.2845845
TTGGGAT41500.036.2168665
CGTTTGG13000.036.1730772
TCTGGGA45200.036.139384
GACCGTT250.002107429836.09
CGACCAA6000.036.029
CGTACAT1000.036.035
CCGCATA250.002107429836.043
Produced by FastQC (version 0.11.5)