##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933188.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1816890 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26663639515876 33.0 31.0 34.0 30.0 34.0 2 32.46891336294437 34.0 31.0 34.0 31.0 34.0 3 32.51113386060796 34.0 31.0 34.0 31.0 34.0 4 35.997107144626256 37.0 35.0 37.0 35.0 37.0 5 35.225415958038184 37.0 35.0 37.0 35.0 37.0 6 34.896742235358225 37.0 35.0 37.0 32.0 37.0 7 35.67075772336245 37.0 35.0 37.0 35.0 37.0 8 35.938149805436765 37.0 35.0 37.0 35.0 37.0 9 37.812749808739106 39.0 38.0 39.0 35.0 39.0 10 37.364223480783096 39.0 37.0 39.0 34.0 39.0 11 37.31402946793697 39.0 37.0 39.0 34.0 39.0 12 37.197104943062044 39.0 37.0 39.0 34.0 39.0 13 37.173800835493616 39.0 37.0 39.0 34.0 39.0 14 38.38604758681043 40.0 38.0 41.0 34.0 41.0 15 38.44716466049128 40.0 38.0 41.0 34.0 41.0 16 38.43759225930023 40.0 38.0 41.0 34.0 41.0 17 38.37439525783069 40.0 38.0 41.0 34.0 41.0 18 38.282575720049095 40.0 37.0 41.0 34.0 41.0 19 38.233819328633 40.0 37.0 41.0 34.0 41.0 20 38.10383127211884 40.0 37.0 41.0 34.0 41.0 21 37.984316056558185 40.0 36.0 41.0 33.0 41.0 22 38.00718040167539 40.0 36.0 41.0 34.0 41.0 23 37.92101393039755 40.0 36.0 41.0 33.0 41.0 24 37.85863150768621 40.0 36.0 41.0 33.0 41.0 25 37.74121603399215 40.0 36.0 41.0 33.0 41.0 26 37.6900979145683 40.0 36.0 41.0 33.0 41.0 27 37.64705843501808 40.0 36.0 41.0 33.0 41.0 28 37.53452162761642 39.0 35.0 41.0 33.0 41.0 29 37.46051109313167 39.0 35.0 41.0 33.0 41.0 30 37.35269609057235 39.0 35.0 41.0 33.0 41.0 31 37.24864796437869 39.0 35.0 41.0 32.0 41.0 32 37.15353103379952 39.0 35.0 41.0 32.0 41.0 33 36.95798975171859 39.0 35.0 41.0 31.0 41.0 34 36.76650430130608 39.0 35.0 41.0 31.0 41.0 35 36.58665576892382 39.0 35.0 41.0 30.0 41.0 36 36.42295460924987 39.0 35.0 41.0 30.0 41.0 37 36.37828432101008 39.0 35.0 41.0 30.0 41.0 38 36.28205835245942 39.0 35.0 41.0 30.0 41.0 39 36.22467458129 39.0 35.0 41.0 30.0 41.0 40 36.10856958869277 39.0 35.0 41.0 30.0 41.0 41 35.964452993852134 39.0 35.0 40.0 29.0 41.0 42 35.85038334736831 38.0 35.0 40.0 29.0 41.0 43 35.71214823131835 38.0 35.0 40.0 28.0 41.0 44 35.638766243415944 38.0 35.0 40.0 28.0 41.0 45 35.62857740424572 38.0 35.0 40.0 28.0 41.0 46 35.42861703240152 38.0 34.0 40.0 27.0 41.0 47 35.338948422854436 38.0 34.0 40.0 27.0 41.0 48 35.24120722773531 38.0 34.0 40.0 27.0 41.0 49 35.27217002680404 37.0 34.0 40.0 27.0 41.0 50 35.08650606255745 37.0 34.0 40.0 27.0 41.0 51 34.03809696789569 36.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 37.0 10 26.0 11 41.0 12 35.0 13 30.0 14 40.0 15 71.0 16 113.0 17 179.0 18 391.0 19 822.0 20 1402.0 21 2317.0 22 3677.0 23 5463.0 24 8335.0 25 13155.0 26 19059.0 27 22907.0 28 24374.0 29 26690.0 30 30989.0 31 37867.0 32 48687.0 33 67147.0 34 108617.0 35 143500.0 36 162729.0 37 236155.0 38 372613.0 39 479262.0 40 155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.975083797037797 4.185338683134367 52.96941476919351 19.870162750634325 2 21.89840881946623 4.746462361507851 51.33937662709355 22.015752191932368 3 19.804446058924867 4.756424439564311 53.41792843815531 22.021201063355512 4 18.19328632993742 4.646731502732691 52.52607477612844 24.633907391201447 5 17.955407316898654 7.768164280721453 49.911717275123976 24.364711127255916 6 21.05091667629851 7.7875380457815275 53.26420421709624 17.897341060823717 7 81.16600344544798 2.2801050146128823 11.98713185718453 4.566759682754597 8 83.81118284541166 3.0022180759429573 9.42308009840992 3.763518980235457 9 78.28817374744756 4.823737265327015 11.406689452856254 5.481399534369169 10 37.981385774592844 27.087935978512732 18.64312093742604 16.28755730946838 11 29.56948411846617 24.463396243030676 26.01016021883548 19.956959419667676 12 25.181106175938005 21.022956810814083 32.04866557689238 21.747271436355533 13 23.382978606299776 20.723764234488602 34.6831673904309 21.21008976878072 14 21.231499980736313 23.16001519079306 33.06259597443984 22.54588885403079 15 20.073697361975682 22.876673876789457 34.925064258155416 22.124564503079437 16 21.957245623015154 21.387811039743738 33.91129897792381 22.743644359317294 17 21.31559973361073 21.275531264963757 32.28335232182465 25.125516679600857 18 22.360021795485693 21.779579391157416 31.715954185448762 24.14444462790813 19 23.75972128197084 23.375988639928668 30.068853920710666 22.795436157389826 20 26.348045286175825 22.912669451645392 29.502666644651026 21.236618617527753 21 25.908117717638383 23.36167847255475 28.801468443328986 21.928735366477884 22 24.010369367435562 22.538293457501553 28.88870542520461 24.562631749858273 23 23.311372730324894 23.97079625073615 28.59391597730187 24.123915041637083 24 22.902046904325523 22.673799734711515 30.27717693421176 24.146976426751205 25 23.835455090842043 23.619536680811716 28.387189097854026 24.157819130492214 26 22.299809014304664 24.913781241572135 28.713846187716374 24.072563556406827 27 22.561464920826246 23.867157615485805 29.31239645768318 24.258981006004767 28 21.744299324670177 24.451507796289263 30.55209726510686 23.252095613933697 29 23.062651013545125 23.85895678879844 29.055749109742475 24.022643087913963 30 23.736274623119726 24.135803488378492 29.111338606079617 23.01658328242216 31 23.297337758477397 25.960790141395464 27.182273004970032 23.55959909515711 32 24.406761003693124 25.650424626697266 27.185135038444812 22.757679331164795 33 24.634898095096567 25.00487096081766 26.551524858411902 23.808706085673872 34 23.342800059442233 25.06238682583976 27.757046381454025 23.837766733263983 35 24.063922416877194 24.72367617192015 26.92023182471146 24.2921695864912 36 23.925774262613587 27.229221361777544 26.097782474448096 22.747221901160774 37 23.902988073025885 26.821766865357837 27.175668312335915 22.099576749280363 38 24.72296066355145 25.030354066564293 27.44002113501643 22.806664134867823 39 24.710576864862485 24.2655306595336 27.10681439162525 23.917078083978666 40 24.756974830617153 23.846462911898904 28.6201145914172 22.77644766606674 41 21.909856953365367 25.118251517703328 28.376566550534154 24.59532497839715 42 22.899405027271875 26.19872419353951 27.238853205202297 23.663017573986316 43 22.510718865754118 25.015163273505824 28.699921294079445 23.774196566660613 44 23.720753595429553 24.16519437060031 28.387849567117435 23.7262024668527 45 23.82912559373435 23.1840672798023 27.49907809498649 25.487729031476864 46 23.292439278107093 24.40197260153339 27.75341380050526 24.552174319854256 47 22.09561393369989 25.172795271040073 29.29803125120398 23.433559544056052 48 22.292103539564863 24.339283060614566 29.002691412248403 24.36592198757217 49 22.545723736714937 23.83859231984325 29.373875138285754 24.24180880515606 50 22.47483336910875 23.05533081254231 29.30094832378405 25.16888749456489 51 21.739290766089304 23.29161369152783 28.074677058049748 26.894418484333123 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 426.0 1 1251.0 2 2076.0 3 13736.0 4 25396.0 5 16797.5 6 8199.0 7 7887.5 8 7576.0 9 7515.5 10 7455.0 11 7301.5 12 7148.0 13 7077.5 14 7007.0 15 6777.0 16 6547.0 17 6130.0 18 5713.0 19 5620.5 20 5528.0 21 5845.0 22 6162.0 23 6860.5 24 7559.0 25 8986.0 26 12215.5 27 14018.0 28 16092.0 29 18166.0 30 20984.0 31 23802.0 32 27830.0 33 31858.0 34 35999.0 35 40140.0 36 44354.5 37 48569.0 38 52848.0 39 57127.0 40 62841.5 41 68556.0 42 76769.5 43 84983.0 44 96454.5 45 107926.0 46 123539.0 47 139152.0 48 151333.5 49 163515.0 50 160065.5 51 156616.0 52 142871.5 53 129127.0 54 117503.0 55 105879.0 56 101850.0 57 97821.0 58 96273.5 59 94726.0 60 90799.5 61 86873.0 62 77753.0 63 68633.0 64 60740.5 65 52848.0 66 45674.5 67 38501.0 68 32753.0 69 27005.0 70 23585.0 71 20165.0 72 16690.0 73 13215.0 74 11039.0 75 7388.5 76 5914.0 77 4404.5 78 2895.0 79 2224.5 80 1554.0 81 1088.5 82 623.0 83 495.5 84 368.0 85 252.0 86 136.0 87 104.5 88 73.0 89 53.0 90 33.0 91 18.0 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1816890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.92297612473439 #Duplication Level Percentage of deduplicated Percentage of total 1 74.35549358419968 22.249376592628483 2 9.308852645447043 5.570971509167649 3 3.314389929374896 2.9752923217423533 4 1.624843317768721 1.944805912161106 5 0.9803776476727231 1.466790847226708 6 0.7809706678277534 1.402137998851664 7 0.6139336540708686 1.2859505451053481 8 0.5318589750128829 1.2731842728828962 9 0.4561696242589317 1.2284957497976163 >10 7.811527558580425 51.5170801060365 >50 0.15869741397217413 3.136646565219846 >100 0.0568601061274797 3.0824423900291107 >500 0.0035772699388473645 0.7698050423407851 >1k 0.0022593283824299147 1.2370739988084716 >5k 0.0 0.0 >10k+ 1.8827736520249288E-4 0.8599461480012985 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15264 0.8401169030596239 No Hit CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT 3143 0.17298790790856905 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC 2408 0.13253416552460523 No Hit GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC 2387 0.13137834431363485 No Hit TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC 2242 0.12339767404741069 No Hit GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC 2238 0.12317751762627348 No Hit CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG 1982 0.10908750667349151 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.7519552642152247E-4 0.0 0.0 0.18410580717599856 0.0 2 2.7519552642152247E-4 0.0 0.0 0.6148418451309655 0.0 3 2.7519552642152247E-4 0.0 0.0 0.9861356493788836 0.0 4 2.7519552642152247E-4 0.0 0.0 1.414780201333047 0.0 5 2.7519552642152247E-4 0.0 0.0 2.240036545965909 0.0 6 2.7519552642152247E-4 0.0 0.0 3.1834618496441722 0.0 7 2.7519552642152247E-4 0.0 0.0 3.912234642713648 0.0 8 2.7519552642152247E-4 0.0 0.0 4.92699062684037 0.0 9 2.7519552642152247E-4 0.0 0.0 5.574525700510213 0.0 10 2.7519552642152247E-4 0.0 0.0 6.5440395400932365 0.0 11 2.7519552642152247E-4 0.0 0.0 7.786107029044135 0.0 12 3.3023463170582697E-4 0.0 0.0 8.928168463693455 0.0 13 3.3023463170582697E-4 0.0 0.0 9.320762401686398 0.0 14 3.3023463170582697E-4 0.0 0.0 9.474431583640177 0.0 15 3.3023463170582697E-4 0.0 0.0 9.679892563666485 0.0 16 3.3023463170582697E-4 0.0 0.0 10.086191238875221 0.0 17 3.3023463170582697E-4 0.0 0.0 10.644397844668637 0.0 18 3.3023463170582697E-4 0.0 0.0 11.2732746616471 0.0 19 3.3023463170582697E-4 0.0 0.0 11.707643280550831 0.0 20 3.3023463170582697E-4 0.0 0.0 12.155441441143934 0.0 21 3.8527373699013146E-4 0.0 0.0 12.730600091364915 0.0 22 3.8527373699013146E-4 0.0 0.0 13.380006494614424 0.0 23 3.8527373699013146E-4 0.0 0.0 14.007837568592485 0.0 24 3.8527373699013146E-4 0.0 0.0 14.518050074577987 0.0 25 3.8527373699013146E-4 0.0 0.0 14.976250626069822 0.0 26 3.8527373699013146E-4 0.0 0.0 15.35277314531975 0.0 27 3.8527373699013146E-4 0.0 0.0 15.747128334681792 0.0 28 3.8527373699013146E-4 0.0 0.0 16.156454160681164 0.0 29 3.8527373699013146E-4 0.0 0.0 16.60722443295962 0.0 30 3.8527373699013146E-4 0.0 0.0 17.109291151363042 0.0 31 3.8527373699013146E-4 0.0 0.0 17.55538309969233 0.0 32 3.8527373699013146E-4 0.0 0.0 18.013033260131323 0.0 33 3.8527373699013146E-4 0.0 0.0 18.4556577448277 0.0 34 4.40312842274436E-4 0.0 0.0 18.898667503261066 0.0 35 5.503910528430449E-4 0.0 0.0 19.366720054598794 0.0 36 5.503910528430449E-4 0.0 0.0 19.808298796294768 0.0 37 5.503910528430449E-4 0.0 0.0 20.249051951411477 0.0 38 5.503910528430449E-4 0.0 0.0 20.69266714000297 0.0 39 5.503910528430449E-4 0.0 0.0 21.158903400866315 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 7640 0.0 42.025524 1 TCGTTTG 630 0.0 38.214287 1 TCGTACA 95 0.0 37.894737 34 TTTGGGA 9135 0.0 37.487686 4 TTTGGGC 4735 0.0 37.064415 4 ACATACG 310 0.0 37.01613 17 CACGACC 585 0.0 36.923077 27 TTACACG 545 0.0 36.743122 34 TGGGCGA 3260 0.0 36.717793 6 GCTACGA 210 0.0 36.42857 10 TACACGC 550 0.0 36.409092 35 CGTTATT 260 0.0 36.346157 1 TTGGGAC 3795 0.0 36.284584 5 TTGGGAT 4150 0.0 36.216866 5 CGTTTGG 1300 0.0 36.173077 2 TCTGGGA 4520 0.0 36.13938 4 GACCGTT 25 0.0021074298 36.0 9 CGACCAA 600 0.0 36.0 29 CGTACAT 100 0.0 36.0 35 CCGCATA 25 0.0021074298 36.0 43 >>END_MODULE