FastQCFastQC Report
Sat 14 Jan 2017
SRR2933187.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933187.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812932
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351831.9406684861870163No Hit
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT40760.22482917175051242Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTT40540.2236156678794351No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT37270.20557858761387632No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC32340.17838506904837026TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC31400.17320009796285796TruSeq Adapter, Index 21 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC31400.17320009796285796No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC31280.17253818676045213TruSeq Adapter, Index 21 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG26610.14677880913349203No Hit
CGTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT22990.12681115452758293No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC22650.12493573945409978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT237500.044.147371
CGTTATT7100.040.246481
CGAATAT800.039.37500414
TCGTTCA2800.039.37516
GTTTTTT270150.039.144922
ACGACCA5150.038.88349528
CGTTTAT2900.038.7931061
CACGACC5250.038.5714327
TACGGGA2800.038.5714264
TCAAGCG5300.038.20754217
AGACACG5350.037.85046824
CGTTCTG4650.037.7419321
AACACGT5000.037.35000241
CGACCAA5500.037.22727229
TGGGCGA30150.036.9402966
TTTGGGC51900.036.4161834
GGGCGAT45500.036.2967037
CACGTGA5100.036.1764743
GACACGA5600.036.16071325
CGAGACA5600.036.16071322
Produced by FastQC (version 0.11.5)