##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933187.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1812932
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08675063377998	33.0	31.0	34.0	30.0	34.0
2	32.329546281934455	34.0	31.0	34.0	30.0	34.0
3	32.28480439420784	33.0	31.0	34.0	30.0	34.0
4	35.899766235027016	37.0	35.0	37.0	35.0	37.0
5	35.11409859829271	37.0	35.0	37.0	35.0	37.0
6	34.8714513285661	37.0	35.0	37.0	32.0	37.0
7	35.657220458351446	37.0	35.0	37.0	33.0	37.0
8	35.924920515496446	37.0	35.0	37.0	35.0	37.0
9	37.78642387028305	39.0	38.0	39.0	35.0	39.0
10	37.32741934060406	39.0	37.0	39.0	34.0	39.0
11	37.27314979271147	39.0	37.0	39.0	34.0	39.0
12	37.15556071601141	39.0	37.0	39.0	34.0	39.0
13	37.12834513373916	39.0	37.0	39.0	33.0	39.0
14	38.23641482416329	40.0	38.0	41.0	33.0	41.0
15	38.31829599786423	40.0	38.0	41.0	33.0	41.0
16	38.31631633177637	40.0	38.0	41.0	33.0	41.0
17	38.26290340729823	40.0	37.0	41.0	33.0	41.0
18	38.037621377966744	39.0	37.0	41.0	33.0	41.0
19	37.824980749415865	39.0	37.0	41.0	34.0	41.0
20	37.51749431307959	39.0	35.0	41.0	33.0	41.0
21	37.39421390322417	39.0	35.0	41.0	33.0	41.0
22	37.45412017659791	39.0	35.0	41.0	33.0	41.0
23	37.38657599954107	39.0	35.0	41.0	33.0	41.0
24	37.309920614783124	39.0	35.0	41.0	33.0	41.0
25	37.210142465354465	39.0	35.0	41.0	33.0	41.0
26	37.19130943686801	39.0	35.0	41.0	33.0	41.0
27	37.17685936372683	39.0	35.0	41.0	33.0	41.0
28	37.060326586987266	39.0	35.0	41.0	33.0	41.0
29	36.957321620446876	39.0	35.0	41.0	33.0	41.0
30	36.776691569237016	39.0	35.0	40.0	32.0	41.0
31	36.5040988851209	39.0	35.0	40.0	31.0	41.0
32	36.12881398750753	39.0	35.0	40.0	30.0	41.0
33	35.59146399313377	38.0	35.0	40.0	26.0	41.0
34	35.04724611844239	38.0	35.0	40.0	23.0	41.0
35	34.67959305699276	38.0	34.0	40.0	20.0	41.0
36	34.43278181421035	38.0	34.0	40.0	18.0	41.0
37	34.34535492781858	38.0	34.0	40.0	18.0	41.0
38	34.20281896949251	38.0	34.0	40.0	18.0	41.0
39	34.156309227262796	38.0	34.0	40.0	17.0	41.0
40	34.02447747626497	38.0	33.0	40.0	16.0	41.0
41	33.83923500715967	38.0	33.0	40.0	15.0	41.0
42	33.73246652384094	38.0	33.0	40.0	15.0	41.0
43	33.614982249747925	38.0	33.0	40.0	15.0	41.0
44	33.54581969980121	38.0	33.0	40.0	15.0	41.0
45	33.5617028106956	37.0	33.0	40.0	15.0	41.0
46	33.3622695170034	37.0	33.0	40.0	14.0	41.0
47	33.26421068192298	37.0	33.0	40.0	14.0	41.0
48	33.13317598233138	37.0	32.0	40.0	13.0	41.0
49	33.18209618452319	37.0	33.0	40.0	13.0	41.0
50	32.99141059896345	37.0	32.0	40.0	12.0	41.0
51	31.975825348110135	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	20.0
10	23.0
11	22.0
12	40.0
13	25.0
14	50.0
15	70.0
16	127.0
17	291.0
18	640.0
19	1287.0
20	2334.0
21	4017.0
22	6147.0
23	10266.0
24	18551.0
25	33929.0
26	49376.0
27	49319.0
28	42564.0
29	37764.0
30	38019.0
31	43440.0
32	52890.0
33	70906.0
34	110333.0
35	133530.0
36	152917.0
37	219474.0
38	348456.0
39	385985.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.030242722837922	3.910792020881092	49.53649668051532	25.522468575765668
2	30.67357187142154	3.7852495294914537	45.56756679235625	19.973611806730755
3	17.571480893933142	3.8992637341058574	57.530177634903026	20.99907773705798
4	15.562139120496521	4.033466230393638	57.61595029488144	22.7884443542284
5	14.831609790107958	7.287807816288752	56.253130288394715	21.62745210520858
6	18.507368174868112	6.834453801907628	58.615712006848575	16.042466016375684
7	72.10275950780283	1.517321112981623	21.5652876114493	4.814631767766248
8	74.56655848095792	1.8112648461166774	19.139327895365078	4.482848777560328
9	68.7596115022516	4.086529445119838	21.645709822541605	5.5081492300869535
10	32.759860822137846	20.56811838502492	29.67187958511406	17.00014120772318
11	25.7991474583713	20.895764430215806	34.411274112873514	18.893813998539382
12	21.31657447714531	18.781289094130393	39.951029602875344	19.951106825848957
13	20.67043882506349	18.926688921592206	42.23837408132241	18.164498172021894
14	18.623478431623468	20.64694097737808	42.223646557068875	18.50593403392957
15	17.522775261289446	20.595973814792835	43.013251462272166	18.867999461645553
16	19.176284604166067	20.025847632453946	40.89077803249101	19.90708973088897
17	19.178711611908223	21.067144272372047	38.81513482028008	20.939009295439654
18	20.43485359627388	20.143281711614115	39.40026432320683	20.021600368905176
19	20.45713794008821	23.57711155189494	36.73474791111857	19.231002596898282
20	20.82505025009212	23.8982488035955	35.97586671756029	19.300834228752098
21	20.731058859350487	24.07260724616257	36.670101250350264	18.52623264413668
22	19.43790500691697	21.52033280895257	37.16278382200767	21.878978362122794
23	18.528328696277633	22.696162900759653	36.32849991064199	22.447008492320727
24	19.00391189520622	22.514688912766722	37.151476172299894	21.32992301972716
25	20.06688612700311	22.69985857163975	35.463878402499375	21.76937689885776
26	18.495122817623606	22.76400879900625	36.78190908428998	21.958959299080163
27	19.047928990166206	21.056333056066084	37.57234137849627	22.32339657527144
28	18.221698331763132	21.981629757762562	37.660430727683114	22.136241182791192
29	19.286934093501575	21.261801325146227	36.66166188251959	22.78960269883261
30	20.9518614046197	20.67501704421346	37.95939395410308	20.41372759706376
31	20.602317130482557	22.526603314410025	36.10361557962461	20.76746397548281
32	20.020993616969637	23.052491764721456	35.01251012172547	21.914004496583438
33	20.948827644942007	22.13916462393515	33.63849278406471	23.273514947058136
34	20.466680493256227	23.02562920175715	33.442898023753784	23.064792281232833
35	20.549088437955753	24.013972945482788	32.22762905613669	23.20930956042477
36	22.15797393393685	25.215452096383096	30.624535283176645	22.00203868650341
37	21.13504532988551	26.061926205726415	30.21492256742117	22.588105896966905
38	20.82940783217462	26.1739546767336	30.055401967641366	22.94123552345041
39	22.30806229908237	23.548208096056552	29.303360523174614	24.84036908168646
40	23.6330982077651	23.18785260561345	30.711025013624337	22.46802417299711
41	20.754391229235296	24.207912927787696	30.928628321415253	24.10906752156176
42	20.179466190678966	24.740255012322578	31.314963826552788	23.765314970445665
43	19.72225102761714	23.22795339262587	32.69819276178036	24.351602817976627
44	20.959032109312428	23.188073242680918	31.44756670410142	24.40532794390523
45	21.393190698823783	22.193717138866763	30.81389704633158	25.599195115977874
46	21.899442450130508	22.406411271906503	31.700085827819247	23.994060450143746
47	20.24350609951173	23.75246286126562	32.48301646173161	23.521014577491048
48	19.31065257825445	23.58935690913945	32.70685276667851	24.39313774592759
49	20.968188547612378	22.17077088385003	32.130107472315565	24.73093309622203
50	20.563815962209283	21.47421966185163	32.38196468483098	25.579999691108107
51	20.028826232864773	21.59446686362202	31.8502845114985	26.5264223920147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	270.0
1	974.5
2	1679.0
3	29138.5
4	56598.0
5	38243.5
6	19889.0
7	19751.0
8	19613.0
9	19972.0
10	20331.0
11	20297.5
12	20264.0
13	19917.0
14	19570.0
15	18667.5
16	17765.0
17	17015.5
18	16266.0
19	15690.0
20	15114.0
21	14699.5
22	14285.0
23	14106.5
24	13928.0
25	13970.5
26	15023.5
27	16034.0
28	16944.0
29	17854.0
30	20230.5
31	22607.0
32	25361.0
33	28115.0
34	32373.5
35	36632.0
36	39556.5
37	42481.0
38	47597.0
39	52713.0
40	58222.0
41	63731.0
42	73385.0
43	83039.0
44	97156.5
45	111274.0
46	136530.0
47	161786.0
48	177362.5
49	192939.0
50	183685.0
51	174431.0
52	150653.5
53	126876.0
54	111421.5
55	95967.0
56	86865.5
57	77764.0
58	70134.5
59	62505.0
60	57650.0
61	52795.0
62	47842.5
63	42890.0
64	38334.5
65	33779.0
66	28966.0
67	24153.0
68	20024.0
69	15895.0
70	12986.5
71	10078.0
72	8533.5
73	6989.0
74	5890.0
75	3706.0
76	2621.0
77	1985.0
78	1349.0
79	979.5
80	610.0
81	464.0
82	318.0
83	243.5
84	169.0
85	138.0
86	107.0
87	69.0
88	31.0
89	21.5
90	12.0
91	7.5
92	3.0
93	5.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1812932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.72281035201687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.77246007456945	30.89340682151926
2	8.729603499672177	6.935287685315614
3	3.305320625383396	3.938898730641434
4	1.7900724990191499	2.8442684157959435
5	1.1572388020367668	2.298438873265084
6	0.8395513335019092	2.0009603040887525
7	0.6714039493155305	1.8669036223779172
8	0.5403778248428439	1.7172260683734144
9	0.46431703966775606	1.659957993893895
>10	4.56618046237498	33.58417513698056
>50	0.10187886037828942	2.7598138316020715
>100	0.05522329567393784	4.228692346188703
>500	0.0039646342174289315	1.0512780528173067
>1k	0.0022655052671022467	2.241819090963149
>5k	0.0	0.0
>10k+	1.4159407919389042E-4	1.9788730261768392
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35183	1.9406684861870163	No Hit
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	4076	0.22482917175051242	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTT	4054	0.2236156678794351	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	3727	0.20557858761387632	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	3234	0.17838506904837026	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	3140	0.17320009796285796	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	3140	0.17320009796285796	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	3128	0.17253818676045213	TruSeq Adapter, Index 21 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	2661	0.14677880913349203	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT	2299	0.12681115452758293	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC	2265	0.12493573945409978	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.5159266867152215E-5	0.0	0.0	0.24143211107752524	0.0
2	5.5159266867152215E-5	0.0	0.0	0.8273338437404161	0.0
3	5.5159266867152215E-5	0.0	0.0	1.3370054695929026	0.0
4	5.5159266867152215E-5	0.0	0.0	1.9953864789192315	0.0
5	5.5159266867152215E-5	0.0	0.0	3.230623101142238	0.0
6	5.5159266867152215E-5	0.0	0.0	5.015411499162682	0.0
7	5.5159266867152215E-5	0.0	0.0	6.443209122018917	0.0
8	5.5159266867152215E-5	0.0	0.0	8.29038265086611	0.0
9	5.5159266867152215E-5	0.0	0.0	9.618121363625331	0.0
10	5.5159266867152215E-5	0.0	0.0	11.180452438370551	0.0
11	5.5159266867152215E-5	0.0	0.0	13.080303067075874	0.0
12	1.1031853373430443E-4	0.0	0.0	14.625424450558542	0.0
13	1.1031853373430443E-4	0.0	0.0	15.28888011243665	0.0
14	1.1031853373430443E-4	0.0	0.0	15.596282706687289	0.0
15	1.1031853373430443E-4	0.0	0.0	15.871196492753176	0.0
16	1.1031853373430443E-4	0.0	0.0	16.357535748720856	0.0
17	1.1031853373430443E-4	0.0	0.0	17.046750788225925	0.0
18	1.1031853373430443E-4	0.0	0.0	17.842588690585195	0.0
19	1.1031853373430443E-4	0.0	0.0	18.354521846379235	0.0
20	1.1031853373430443E-4	0.0	0.0	18.894144954140586	0.0
21	1.1031853373430443E-4	0.0	0.0	19.603603444585897	0.0
22	1.1031853373430443E-4	0.0	0.0	20.333912137906992	0.0
23	1.6547780060145666E-4	0.0	0.0	21.080161859352696	0.0
24	1.6547780060145666E-4	0.0	0.0	21.66192664700055	0.0
25	1.6547780060145666E-4	0.0	0.0	22.172039546987975	0.0
26	1.6547780060145666E-4	0.0	0.0	22.630854328788946	0.0
27	1.6547780060145666E-4	0.0	0.0	23.10958160592896	0.0
28	1.6547780060145666E-4	0.0	0.0	23.606952715270072	0.0
29	1.6547780060145666E-4	0.0	0.0	24.158104109806654	0.0
30	1.6547780060145666E-4	0.0	0.0	24.78118318833801	0.0
31	1.6547780060145666E-4	0.0	0.0	25.305913294045226	0.0
32	1.6547780060145666E-4	0.0	0.0	25.840903023389735	0.0
33	1.6547780060145666E-4	0.0	0.0	26.36612956249876	0.0
34	1.6547780060145666E-4	0.0	0.0	26.879662337031945	0.0
35	1.6547780060145666E-4	0.0	0.0	27.434840358049833	0.0
36	1.6547780060145666E-4	0.0	0.0	27.946773513843873	0.0
37	1.6547780060145666E-4	0.0	0.0	28.457493165766834	0.0
38	1.6547780060145666E-4	0.0	0.0	29.036058715936395	0.0
39	1.6547780060145666E-4	0.0	0.0	29.785121559992323	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	23750	0.0	44.14737	1
CGTTATT	710	0.0	40.24648	1
CGAATAT	80	0.0	39.375004	14
TCGTTCA	280	0.0	39.375	16
GTTTTTT	27015	0.0	39.14492	2
ACGACCA	515	0.0	38.883495	28
CGTTTAT	290	0.0	38.793106	1
CACGACC	525	0.0	38.57143	27
TACGGGA	280	0.0	38.571426	4
TCAAGCG	530	0.0	38.207542	17
AGACACG	535	0.0	37.850468	24
CGTTCTG	465	0.0	37.741932	1
AACACGT	500	0.0	37.350002	41
CGACCAA	550	0.0	37.227272	29
TGGGCGA	3015	0.0	36.940296	6
TTTGGGC	5190	0.0	36.416183	4
GGGCGAT	4550	0.0	36.296703	7
CACGTGA	510	0.0	36.17647	43
GACACGA	560	0.0	36.160713	25
CGAGACA	560	0.0	36.160713	22
>>END_MODULE