FastQCFastQC Report
Sat 14 Jan 2017
SRR2933186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843400
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166080.9009439079960941No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC37820.20516437018552677No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC32680.1772811109905609No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG32370.1755994358251058No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT31580.1713138765324943Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC28630.15531083866767928No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC20740.11250949332754692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG253.8917264E-545.04
CGTTTTT89300.042.001681
CAACCCG1000.038.2523
TACGGGA3100.037.7419364
TCAAGCG17600.037.58522817
TTACGGG5250.037.2857173
CACGACC17100.037.10526327
GCGAGAC17550.037.05128521
TTTGGGA87650.037.016544
CGACCAA16850.036.7210729
CCGGTAT800.036.562542
AAACACG15850.036.4826540
AGACACG17550.036.4102624
ACATACG3400.036.39705717
AACACGT15550.036.3183341
ACGACCA17100.036.1842128
ACACGAC17500.036.1285726
TTTGGGC44250.035.9999964
TTGGGAT40300.035.787845
CGTTTGG12350.035.7085042
Produced by FastQC (version 0.11.5)