##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933183.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1773399
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17966289594163	33.0	31.0	34.0	30.0	34.0
2	32.368717925294874	34.0	31.0	34.0	30.0	34.0
3	32.44298378424709	34.0	31.0	34.0	30.0	34.0
4	35.9308339522014	37.0	35.0	37.0	35.0	37.0
5	35.10860556479393	37.0	35.0	37.0	35.0	37.0
6	34.8216616790694	37.0	35.0	37.0	32.0	37.0
7	35.62317673574869	37.0	35.0	37.0	33.0	37.0
8	35.916257424302145	37.0	35.0	37.0	35.0	37.0
9	37.785505687101434	39.0	38.0	39.0	35.0	39.0
10	37.285282105155126	39.0	37.0	39.0	34.0	39.0
11	37.18741524045068	39.0	37.0	39.0	34.0	39.0
12	36.94118074950984	39.0	37.0	39.0	33.0	39.0
13	36.83201637082236	39.0	35.0	39.0	33.0	39.0
14	37.721381933789296	40.0	37.0	41.0	33.0	41.0
15	37.87888230454624	40.0	37.0	41.0	33.0	41.0
16	37.95566254407497	40.0	37.0	41.0	33.0	41.0
17	37.9195505354407	40.0	36.0	41.0	33.0	41.0
18	37.91950260488474	40.0	36.0	41.0	33.0	41.0
19	37.89280528521782	40.0	36.0	41.0	33.0	41.0
20	37.76384333136536	40.0	36.0	41.0	33.0	41.0
21	37.637960774760785	40.0	36.0	41.0	33.0	41.0
22	37.74105940061994	39.0	36.0	41.0	33.0	41.0
23	37.663680311086225	39.0	35.0	41.0	33.0	41.0
24	37.59606664941167	39.0	35.0	41.0	33.0	41.0
25	37.481238570676986	39.0	35.0	41.0	33.0	41.0
26	37.43911043143703	39.0	35.0	41.0	33.0	41.0
27	37.399970339444195	39.0	35.0	41.0	33.0	41.0
28	37.300197530279426	39.0	35.0	41.0	32.0	41.0
29	37.22158239629096	39.0	35.0	41.0	32.0	41.0
30	37.11433467595279	39.0	35.0	41.0	32.0	41.0
31	36.97298915810824	39.0	35.0	41.0	31.0	41.0
32	36.92080067711778	39.0	35.0	41.0	31.0	41.0
33	36.79119419825995	39.0	35.0	41.0	31.0	41.0
34	36.58421539653513	39.0	35.0	41.0	31.0	41.0
35	36.443904614810315	39.0	35.0	41.0	30.0	41.0
36	36.30927614146619	39.0	35.0	40.0	30.0	41.0
37	36.2495270381905	39.0	35.0	40.0	30.0	41.0
38	36.11474236762285	38.0	35.0	40.0	30.0	41.0
39	36.06661219499954	38.0	35.0	40.0	30.0	41.0
40	35.9216701937917	38.0	35.0	40.0	29.0	41.0
41	35.74613552844002	38.0	35.0	40.0	29.0	41.0
42	35.684931591818874	38.0	35.0	40.0	29.0	41.0
43	35.57363571311363	38.0	34.0	40.0	28.0	41.0
44	35.499637137496975	38.0	34.0	40.0	28.0	41.0
45	35.4861714707181	38.0	34.0	40.0	28.0	41.0
46	35.2790313967697	37.0	34.0	40.0	28.0	41.0
47	35.16348999858464	37.0	34.0	40.0	27.0	41.0
48	35.0705819728104	37.0	34.0	40.0	27.0	41.0
49	35.13396759556084	37.0	34.0	40.0	27.0	41.0
50	34.92663692716642	37.0	34.0	40.0	27.0	41.0
51	33.79701917053072	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	25.0
10	29.0
11	29.0
12	30.0
13	35.0
14	40.0
15	70.0
16	109.0
17	245.0
18	495.0
19	878.0
20	1481.0
21	2479.0
22	3661.0
23	5379.0
24	8350.0
25	12479.0
26	16833.0
27	20638.0
28	23694.0
29	27627.0
30	33715.0
31	43447.0
32	56884.0
33	80817.0
34	130363.0
35	143428.0
36	160878.0
37	235793.0
38	362944.0
39	400364.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.105969384216408	4.267003646669474	52.89903738526975	16.72798958384436
2	21.334792677789938	4.259955035499625	51.49123237353804	22.914019913172385
3	22.275302963405302	4.645316705377639	50.632542366382296	22.446837964834764
4	18.908773490906448	4.85750809603479	50.484747087372895	25.748971325685872
5	17.777612370368992	7.850179232084827	50.22693708522448	24.145271312321707
6	22.035086294736832	7.547145340670655	52.32234821379734	18.095420150795167
7	83.61000541897226	2.075956961744086	9.997637305535868	4.31640031374778
8	86.13301349555289	2.215463074017748	7.792380620492061	3.8591428099373015
9	80.66148678329017	4.599021427214067	9.829034526353066	4.9104572631427
10	40.45372755933661	26.83214550137899	16.717444861534265	15.99668207775013
11	32.47560193729668	23.20543769337865	24.53446742667612	19.78449294264855
12	27.133656892780472	21.5784490687093	30.740403033947807	20.547491004562424
13	25.408326045069384	22.1967532405285	33.0555616643519	19.339359050050213
14	21.887347404616783	22.560179632445944	35.12655640383242	20.42591655910486
15	20.720548506004572	20.752464617381648	36.13676335669525	22.39022351991853
16	24.94982798569301	21.348100455678615	31.718863042101635	21.983208516526737
17	24.345282702877356	21.22511628798708	30.443233587026945	23.986367422108618
18	26.696191889134933	19.824529054093297	30.46595830943854	23.01332074733323
19	25.66139938051166	23.233068249164457	28.28573829127004	22.81979407905384
20	26.414529386787745	25.608732157850543	27.38870383935031	20.5880346160114
21	26.142283828963475	24.966067985828346	28.590012738250103	20.301635446958073
22	24.754214928507345	21.307218510893488	29.772938859218932	24.16562770138023
23	23.290415749642353	24.331298258316377	29.173412187556213	23.20487380448506
24	22.89185907965438	24.545519648990442	29.368123022512137	23.19449824884304
25	24.5465346489989	24.988961874908018	26.574673832566724	23.889829643526358
26	22.843477412584534	24.43217798137926	26.892650779660976	25.831693826375222
27	22.46978824280379	22.219421574050735	30.119617750996813	25.19117243214866
28	19.997699333314163	25.5452946573219	28.35464551406649	26.10236049529745
29	22.197091573864654	25.16985743197103	27.096947725807897	25.536103268356413
30	25.253876877115637	22.871728246153292	28.31957162488532	23.55482325184575
31	24.955297707960813	25.915938827077266	26.199913273888164	22.928850191073753
32	25.41881437849012	24.84635437371962	25.49792799026051	24.23690325752975
33	24.807784373398203	24.19010047936195	24.55995520466629	26.442159942573557
34	22.009316572299863	26.343592163974378	27.749085231242375	23.898006032483384
35	21.78956907046863	28.431503570262528	25.62869382468356	24.15023353458528
36	24.86986854058224	28.46826912612446	23.46285297330155	23.199009359991745
37	23.099257414716035	28.22715023522625	24.14453825676004	24.529054093297674
38	24.040613533671777	27.426991895224933	25.190890487701868	23.341504083401425
39	24.27293575783002	24.937929930038305	24.610648816199852	26.178485495931824
40	26.522288554352404	24.925411596600654	26.200082440556244	22.352217408490702
41	22.4403532425585	25.907029382558576	25.47695132341904	26.175666051463885
42	23.039654358663785	25.658072436039493	26.693598000224426	24.608675205072295
43	22.275697685630814	23.60585519671546	28.073998011727763	26.044449105925967
44	23.72912130885379	24.411314092316506	26.76797494528868	25.091589653541025
45	23.539541862829516	23.101174636954234	25.381992433738827	27.977291066477427
46	23.406069361717243	23.61284741899595	26.964321058036013	26.016762161250796
47	20.674873505623946	26.22173577407002	29.132530242771086	23.97086047753495
48	21.72534212548896	26.826111890217597	27.366994116947176	24.081551867346267
49	24.023978811310933	24.849906873749227	27.105236892543637	24.0208774223962
50	22.0223987946311	24.968718263628208	26.80541716782292	26.203465773917774
51	22.01083907231255	24.44204603701705	25.359662433552742	28.18745245711766
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	196.0
1	1022.0
2	1848.0
3	8046.0
4	14244.0
5	9273.0
6	4302.0
7	4253.0
8	4204.0
9	4338.0
10	4472.0
11	4419.0
12	4366.0
13	4265.0
14	4164.0
15	4187.0
16	4210.0
17	4470.5
18	4731.0
19	4660.5
20	4590.0
21	4913.0
22	5236.0
23	5589.0
24	5942.0
25	7058.0
26	9635.0
27	11096.0
28	12907.5
29	14719.0
30	17288.5
31	19858.0
32	24340.5
33	28823.0
34	33178.5
35	37534.0
36	40464.0
37	43394.0
38	46887.0
39	50380.0
40	55833.5
41	61287.0
42	69696.5
43	78106.0
44	88361.5
45	98617.0
46	115696.5
47	132776.0
48	151715.0
49	170654.0
50	173112.0
51	175570.0
52	163169.5
53	150769.0
54	139299.0
55	127829.0
56	120357.0
57	112885.0
58	103991.5
59	95098.0
60	83865.5
61	72633.0
62	68198.0
63	63763.0
64	56695.0
65	49627.0
66	42416.5
67	35206.0
68	29624.0
69	24042.0
70	20672.5
71	17303.0
72	14619.5
73	11936.0
74	10088.5
75	7024.0
76	5807.0
77	4177.0
78	2547.0
79	1927.5
80	1308.0
81	971.0
82	634.0
83	401.5
84	169.0
85	111.5
86	54.0
87	44.0
88	34.0
89	22.5
90	11.0
91	9.5
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1773399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.178847476237838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30469974288681	23.79092595003592
2	9.56620608256287	5.965265607489727
3	3.3621624804603414	3.1448505350580738
4	1.6234110660925145	2.0246434408374063
5	0.9863968289989311	1.5377358141201163
6	0.7118994648516205	1.3317722899814406
7	0.5103783336199371	1.1139105753378713
8	0.41306157883563793	1.0303027171848274
9	0.3595545940352888	1.0089448062126196
>10	5.656825345645669	43.63794250276242
>50	0.4214639600609525	8.08648844064147
>100	0.07688519061918596	4.537964103827608
>500	0.004703554220155557	0.9991472665318637
>1k	0.0021708711785333344	1.3116839608896065
>5k	1.8090593154444456E-4	0.47842198908904077
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8482	0.4782905595413102	No Hit
GCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCTGC	3507	0.19775583498129862	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCTGCT	3170	0.17875277926738428	Illumina Single End Adapter 1 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCTGC	2848	0.16059555689385185	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTCTGC	2199	0.12399916769999307	Illumina Single End Adapter 1 (95% over 22bp)
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	2186	0.1232661121383287	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.691666680763889E-4	0.0	0.0	0.19228611271349538	0.0
2	1.691666680763889E-4	0.0	0.0	0.7305180616432061	0.0
3	1.691666680763889E-4	0.0	0.0	1.1030794536367732	0.0
4	1.691666680763889E-4	0.0	0.0	1.5704305686424769	0.0
5	1.691666680763889E-4	0.0	0.0	2.402166686684722	0.0
6	1.691666680763889E-4	0.0	0.0	3.2293916935782643	0.0
7	1.691666680763889E-4	0.0	0.0	3.9458125328817713	0.0
8	1.691666680763889E-4	0.0	0.0	5.082161431240234	0.0
9	1.691666680763889E-4	0.0	0.0	5.8415506042351435	0.0
10	1.691666680763889E-4	0.0	0.0	6.936622835582968	0.0
11	1.691666680763889E-4	0.0	0.0	8.476716181750412	0.0
12	1.691666680763889E-4	0.0	0.0	9.754826747956889	0.0
13	1.691666680763889E-4	0.0	0.0	10.221106474064776	0.0
14	1.691666680763889E-4	0.0	0.0	10.414238420118654	0.0
15	1.691666680763889E-4	0.0	0.0	10.640414255336786	0.0
16	1.691666680763889E-4	0.0	0.0	11.122764814911928	0.0
17	1.691666680763889E-4	0.0	0.0	11.795315098294292	0.0
18	1.691666680763889E-4	0.0	0.0	12.523803159920583	0.0
19	1.691666680763889E-4	0.0	0.0	12.999725386108823	0.0
20	1.691666680763889E-4	0.0	0.0	13.552674835161179	0.0
21	1.691666680763889E-4	0.0	0.0	14.290579841310388	0.0
22	1.691666680763889E-4	0.0	0.0	15.07179151448715	0.0
23	1.691666680763889E-4	0.0	0.0	15.805862076159961	0.0
24	1.691666680763889E-4	0.0	0.0	16.374487636454063	0.0
25	1.691666680763889E-4	0.0	0.0	16.88300264069169	0.0
26	1.691666680763889E-4	0.0	0.0	17.338060977817175	0.0
27	1.691666680763889E-4	0.0	0.0	17.82452792631551	0.0
28	2.255555574351852E-4	0.0	0.0	18.29311959688711	0.0
29	2.255555574351852E-4	0.0	0.0	18.86636904610863	0.0
30	2.255555574351852E-4	0.0	0.0	19.609123496742697	0.0
31	2.255555574351852E-4	0.0	0.0	20.11622877874635	0.0
32	2.255555574351852E-4	0.0	0.0	20.636077949745093	0.0
33	3.383333361527778E-4	0.0	0.0	21.167712398619827	0.0
34	3.947222255115741E-4	0.0	0.0	21.727090181059086	0.0
35	3.947222255115741E-4	0.0	0.0	22.424169631312523	0.0
36	3.947222255115741E-4	0.0	0.0	22.982927135968836	0.0
37	3.947222255115741E-4	0.0	0.0	23.504298806980266	0.0
38	3.947222255115741E-4	0.0	0.0	24.14606075677273	0.0
39	3.947222255115741E-4	0.0	0.0	25.322332988797218	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	4890	0.0	41.18098	1
GTCGATA	35	6.2502077E-6	38.57143	41
TATTGCG	105	0.0	38.57143	1
TACGCGG	105	0.0	38.57143	2
TTTGGGA	8205	0.0	38.418648	4
CTTTACG	65	9.094947E-12	38.07692	1
ACACGAC	2055	0.0	37.773724	26
AGACACG	2055	0.0	37.773724	24
TACGGGA	215	0.0	37.67442	4
TTGGGAC	2945	0.0	37.512737	5
TTGTTCG	390	0.0	37.500004	1
ACGGGTA	30	1.1401719E-4	37.499996	5
ATATCGG	30	1.1401719E-4	37.499996	29
CGTTTGG	1250	0.0	37.26	2
CACGACC	2085	0.0	37.230217	27
CGAGACA	2135	0.0	37.096016	22
TTACACG	2985	0.0	37.01005	34
GACACGA	2100	0.0	36.964287	25
ATTCCCG	55	2.750312E-9	36.81818	1
AAACACG	1975	0.0	36.79747	40
>>END_MODULE