SRR2933181.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933181.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150772
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	4258	0.370012478579597	Illumina PCR Primer Index 11 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3683	0.32004602127962795	Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3325	0.28893647047373416	Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3189	0.2771183170949589	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2724	0.2367106603219404	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	2636	0.2290636199003799	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	2562	0.2226331540913404	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	2320	0.20160379293204908	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1354	0.11766014466810107	Illumina PCR Primer Index 11 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	7.0332916E-4	45.0	9
CGTACTC	40	6.8157533E-9	45.0	45
CGTACAC	20	7.0332916E-4	45.0	19
TCGACTA	20	7.0332916E-4	45.0	33
TACGTTC	25	3.890809E-5	45.0	31
TTTACGG	205	0.0	41.70732	2
CTTACGG	65	0.0	41.538464	2
CGTTATT	60	3.6379788E-12	41.250004	1
TTACGGG	300	0.0	40.5	3
TACGGGA	175	0.0	39.857143	4
TACGGGT	40	3.4582263E-7	39.375	4
CGTTTTT	1735	0.0	39.034584	1
TAATGCG	110	0.0	38.863636	24
TCTTGCG	365	0.0	38.835617	1
TTGGGAC	2235	0.0	38.65772	5
CGCATCG	35	6.248296E-6	38.571426	21
GTATGCG	35	6.248296E-6	38.571426	1
TACGAAT	130	0.0	38.076927	12
CGGTAGT	220	0.0	37.840908	12
CGAATAT	125	0.0	37.800003	14