##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933181.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150772
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11360026138974	33.0	31.0	34.0	30.0	34.0
2	32.305289840211614	34.0	31.0	34.0	30.0	34.0
3	32.38430983722232	34.0	31.0	34.0	30.0	34.0
4	35.87948698786554	37.0	35.0	37.0	35.0	37.0
5	35.09675591689753	37.0	35.0	37.0	35.0	37.0
6	34.76615437289055	37.0	35.0	37.0	32.0	37.0
7	35.59933766202167	37.0	35.0	37.0	33.0	37.0
8	35.86550072473088	37.0	35.0	37.0	35.0	37.0
9	37.75007473244048	39.0	38.0	39.0	35.0	39.0
10	37.20030900995158	39.0	37.0	39.0	34.0	39.0
11	37.10572554771927	39.0	37.0	39.0	33.0	39.0
12	36.923206334530214	39.0	37.0	39.0	33.0	39.0
13	36.880637519856236	39.0	37.0	39.0	33.0	39.0
14	37.97366463556639	40.0	37.0	41.0	33.0	41.0
15	38.053655285321504	40.0	37.0	41.0	33.0	41.0
16	38.05678709596688	40.0	37.0	41.0	33.0	41.0
17	37.98395511882458	40.0	37.0	41.0	33.0	41.0
18	37.971402675768964	40.0	37.0	41.0	33.0	41.0
19	37.98517603834643	40.0	37.0	41.0	33.0	41.0
20	37.8811432673023	40.0	36.0	41.0	33.0	41.0
21	37.76558779671386	40.0	36.0	41.0	33.0	41.0
22	37.82439962042872	40.0	36.0	41.0	33.0	41.0
23	37.75116964959175	39.0	36.0	41.0	33.0	41.0
24	37.70698365966499	39.0	36.0	41.0	33.0	41.0
25	37.61056316976777	39.0	36.0	41.0	33.0	41.0
26	37.59477029333352	39.0	36.0	41.0	33.0	41.0
27	37.54276607355758	39.0	36.0	41.0	33.0	41.0
28	37.468353418400866	39.0	36.0	41.0	33.0	41.0
29	37.40192149270229	39.0	35.0	41.0	32.0	41.0
30	37.27944371256861	39.0	35.0	41.0	32.0	41.0
31	37.210529105678624	39.0	35.0	41.0	32.0	41.0
32	37.196070985390676	39.0	35.0	41.0	32.0	41.0
33	37.108989443608294	39.0	35.0	41.0	32.0	41.0
34	36.99258584671855	39.0	35.0	41.0	31.0	41.0
35	36.877049493731164	39.0	35.0	41.0	31.0	41.0
36	36.73092758600313	39.0	35.0	41.0	31.0	41.0
37	36.673096842815085	39.0	35.0	41.0	31.0	41.0
38	36.59491975821449	39.0	35.0	40.0	31.0	41.0
39	36.552541250569185	39.0	35.0	40.0	31.0	41.0
40	36.47996214714991	39.0	35.0	40.0	30.0	41.0
41	36.35773984768486	39.0	35.0	40.0	30.0	41.0
42	36.29870904053974	38.0	35.0	40.0	30.0	41.0
43	36.13959672289559	38.0	35.0	40.0	30.0	41.0
44	36.06306896587682	38.0	35.0	40.0	30.0	41.0
45	36.032550322739866	38.0	35.0	40.0	30.0	41.0
46	35.76439033970239	38.0	35.0	40.0	29.0	41.0
47	35.652428978112084	38.0	34.0	40.0	28.0	41.0
48	35.57290323365532	38.0	34.0	40.0	28.0	41.0
49	35.667151268887324	38.0	34.0	40.0	29.0	41.0
50	35.44422613688898	37.0	34.0	40.0	28.0	41.0
51	34.28231048374482	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	8.0
9	14.0
10	22.0
11	24.0
12	17.0
13	20.0
14	18.0
15	34.0
16	68.0
17	95.0
18	203.0
19	385.0
20	708.0
21	1276.0
22	1957.0
23	2961.0
24	4326.0
25	6553.0
26	8746.0
27	10804.0
28	13121.0
29	15966.0
30	20133.0
31	26160.0
32	34081.0
33	47685.0
34	77617.0
35	99471.0
36	103833.0
37	151510.0
38	242781.0
39	280070.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.121980722506283	4.467001282617234	54.545209650565006	16.86580834431147
2	19.844069893949452	5.598589468634969	52.56375719951476	21.993583437900817
3	20.829147737345018	5.685313858870393	51.35509032197516	22.13044808180943
4	18.59586434150292	4.64705432527034	51.850844476577464	24.906236856649276
5	18.067784061482207	8.065542088267701	49.48269509511876	24.383978755131338
6	20.982088545776225	8.29721265376634	53.2327863382147	17.48791246224274
7	82.42970805685226	2.006739823353366	10.5130295141001	5.0505226056942645
8	84.8041141077468	3.1058280875794684	7.37461460654239	4.715443198131341
9	79.35455502914566	4.784961747418255	9.467731227384748	6.392751996051346
10	38.95810812219971	28.22496550141992	16.843649306726267	15.97327706965411
11	30.284539422231337	24.980013417080013	24.919792973760224	19.815654186928427
12	24.77467300212379	22.76645590959808	31.214002426197368	21.24486866208076
13	23.599288130055303	23.110138237635258	33.19493348812797	20.09564014418147
14	21.168311359678547	25.510613744512376	31.776668184488326	21.54440671132075
15	19.56895023514649	23.12282537288012	35.35226786887411	21.955956523099278
16	21.098532115831805	23.158279833016447	31.831066449305332	23.912121601846412
17	20.552811503929536	22.807211159117532	30.3163441585301	26.323633178422835
18	22.41443135564647	22.16294800360106	31.587143239494882	23.83547740125759
19	23.263687333372726	24.70758760206192	29.177456524837243	22.85126853972811
20	26.342924575849953	24.935608443722995	28.024317588540566	20.69714939188649
21	24.798656901627776	26.451634207297364	28.20680378041871	20.54290511065615
22	23.419843374708456	23.427490415130016	28.193160765121156	24.95950544504037
23	21.776424869565822	25.526168519915327	27.44340321106179	25.25400339945706
24	22.508368295370413	24.125717344530454	29.500978473581213	23.86493588651792
25	21.746879486118882	25.794336323789597	27.421939358969453	25.036844831122064
26	21.74913883897071	25.088288557594378	27.99503289965345	25.16753970378146
27	20.98956178982457	24.472788701845367	29.459180445822458	25.0784690625076
28	19.752566103450555	24.525622799303424	31.89119999443852	23.830611102807506
29	21.63330355622139	23.431053240780972	29.306413433764465	25.629229769233174
30	22.084478941093458	24.19184686453963	30.60927794558783	23.11439624877908
31	23.44904116540896	26.42938827152555	27.214947878467672	22.906622684597817
32	23.755444171391034	26.769855366658206	25.91990420343908	23.554796258511676
33	22.66600160587849	24.334794381510847	26.69303736969617	26.306166642914498
34	21.443517916668114	24.859833225000262	28.473841907867065	25.22280695046456
35	22.321884786908267	24.76711285988884	28.105306698459813	24.805695654743076
36	23.170966968261304	25.138602607640788	27.691410635642853	23.999019788455055
37	20.903011195962364	26.856058367773983	29.871859934026894	22.36907050223676
38	20.855825480633868	28.09114229404261	27.315749774933696	23.737282450389827
39	21.367221308825727	26.252724258150177	26.753257813015956	25.626796620008136
40	22.950245574275353	24.77588957673631	27.185663189580563	25.088201659407773
41	21.575950753059686	25.03380339459076	26.362911158769936	27.027334693579615
42	22.554163639713167	23.329034769702425	27.53690565985269	26.579895930731716
43	21.71272849878169	23.004730737278976	28.724108685299953	26.55843207863938
44	21.143284681935256	23.613278738099293	29.1167146923978	26.12672188756765
45	22.08108991181572	23.73519689391122	27.286204391486756	26.8975088027863
46	22.92765204575711	24.20896580730153	28.596542147358466	24.26683999958289
47	20.29750463167335	24.956811601255506	30.09675244097006	24.648931326101085
48	20.24493122877512	24.60061593434668	29.953978720372064	25.20047411650614
49	22.096644687218667	22.225862290705717	31.02134914648601	24.656143875589603
50	21.39728808139232	22.750640439635305	28.799623209462865	27.05244826950951
51	20.357638176806525	22.53348187130031	27.538296030838428	29.570583921054734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	663.5
2	1108.0
3	3088.0
4	5068.0
5	3480.5
6	1893.0
7	1920.5
8	1948.0
9	2024.5
10	2101.0
11	2100.0
12	2099.0
13	2236.5
14	2374.0
15	2293.5
16	2213.0
17	2358.0
18	2503.0
19	2550.0
20	2597.0
21	2893.5
22	3190.0
23	3647.5
24	4105.0
25	5186.5
26	7148.5
27	8029.0
28	9314.5
29	10600.0
30	12162.0
31	13724.0
32	17038.0
33	20352.0
34	23619.0
35	26886.0
36	29449.0
37	32012.0
38	34842.0
39	37672.0
40	43988.0
41	50304.0
42	57300.5
43	64297.0
44	72798.0
45	81299.0
46	97461.5
47	113624.0
48	122012.5
49	130401.0
50	125715.0
51	121029.0
52	105090.5
53	89152.0
54	79073.0
55	68994.0
56	63141.5
57	57289.0
58	52702.5
59	48116.0
60	43551.0
61	38986.0
62	34965.0
63	30944.0
64	26621.0
65	22298.0
66	19099.5
67	15901.0
68	13592.0
69	11283.0
70	9179.5
71	7076.0
72	5998.0
73	4920.0
74	4367.5
75	2957.0
76	2099.0
77	1612.5
78	1126.0
79	819.0
80	512.0
81	356.5
82	201.0
83	139.0
84	77.0
85	48.5
86	20.0
87	18.5
88	17.0
89	20.5
90	24.0
91	14.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1150772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.1424773685372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0027308432701	25.94921517763374
2	10.689162650906027	7.299089877943442
3	3.8208924444554753	3.9136420143730763
4	1.760755661262458	2.404662412647089
5	1.0330371164306904	1.7635223184296889
6	0.6255593405468386	1.2814887376378494
7	0.43371862848130444	1.0365759920065154
8	0.3405040777699186	0.9300522215323261
9	0.2788750099527963	0.8569335344367542
>10	4.579619218740385	40.18768030390268
>50	0.3694187188333918	7.699123482175736
>100	0.06004318915054079	3.667601222597384
>500	0.0032121870695939188	0.7270411581931575
>1k	0.002470913130456861	2.283371546490601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	4258	0.370012478579597	Illumina PCR Primer Index 11 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3683	0.32004602127962795	Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3325	0.28893647047373416	Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3189	0.2771183170949589	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2724	0.2367106603219404	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	2636	0.2290636199003799	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	2562	0.2226331540913404	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	2320	0.20160379293204908	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1354	0.11766014466810107	Illumina PCR Primer Index 11 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6069455982592553E-4	0.0	0.0	0.4006875384524476	0.0
2	2.6069455982592553E-4	0.0	0.0	1.3983656189062648	0.0
3	2.6069455982592553E-4	0.0	0.0	2.0297678428046564	0.0
4	2.6069455982592553E-4	0.0	0.0	2.799511979784006	0.0
5	2.6069455982592553E-4	0.0	0.0	4.1244486310059685	0.0
6	2.6069455982592553E-4	0.0	0.0	4.944854410778156	0.0
7	2.6069455982592553E-4	0.0	0.0	5.75717865919574	0.0
8	2.6069455982592553E-4	0.0	0.0	7.045183581109029	0.0
9	2.6069455982592553E-4	0.0	0.0	7.734286200915559	0.0
10	2.6069455982592553E-4	0.0	0.0	9.054964840993698	0.0
11	2.6069455982592553E-4	0.0	0.0	10.901725102800555	0.0
12	2.6069455982592553E-4	0.0	0.0	12.822609517784583	0.0
13	2.6069455982592553E-4	0.0	0.0	13.376411661041457	0.0
14	2.6069455982592553E-4	0.0	0.0	13.577059573920812	0.0
15	2.6069455982592553E-4	0.0	0.0	13.888502674726183	0.0
16	2.6069455982592553E-4	0.0	0.0	14.558922184411855	0.0
17	3.475927464345674E-4	0.0	0.0	15.543739333247593	0.0
18	3.475927464345674E-4	0.0	0.0	16.618843697969712	0.0
19	3.475927464345674E-4	0.0	0.0	17.39614797718401	0.0
20	3.475927464345674E-4	0.0	0.0	18.117924315155392	0.0
21	3.475927464345674E-4	0.0	0.0	19.108042253374258	0.0
22	3.475927464345674E-4	0.0	0.0	20.094075976822516	0.0
23	3.475927464345674E-4	0.0	0.0	21.132161714049353	0.0
24	3.475927464345674E-4	0.0	0.0	21.9300608634899	0.0
25	3.475927464345674E-4	0.0	0.0	22.608040515410526	0.0
26	3.475927464345674E-4	0.0	0.0	23.200251657148417	0.0
27	3.475927464345674E-4	0.0	0.0	23.79524354085779	0.0
28	3.475927464345674E-4	0.0	0.0	24.44211364197252	0.0
29	3.475927464345674E-4	0.0	0.0	25.141730942358695	0.0
30	3.475927464345674E-4	0.0	0.0	25.922163556290908	0.0
31	3.475927464345674E-4	0.0	0.0	26.565992220874335	0.0
32	3.475927464345674E-4	0.0	0.0	27.16958702505796	0.0
33	3.475927464345674E-4	0.0	0.0	27.80116304532957	0.0
34	3.475927464345674E-4	0.0	0.0	28.447772451884475	0.0
35	3.475927464345674E-4	0.0	0.0	29.136614377131178	0.0
36	3.475927464345674E-4	0.0	0.0	29.78209410726017	0.0
37	3.475927464345674E-4	0.0	0.0	30.401938872339613	0.0
38	3.475927464345674E-4	0.0	0.0	31.05019934444008	0.0
39	3.475927464345674E-4	0.0	0.0	31.718880890393578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	7.0332916E-4	45.0	9
CGTACTC	40	6.8157533E-9	45.0	45
CGTACAC	20	7.0332916E-4	45.0	19
TCGACTA	20	7.0332916E-4	45.0	33
TACGTTC	25	3.890809E-5	45.0	31
TTTACGG	205	0.0	41.70732	2
CTTACGG	65	0.0	41.538464	2
CGTTATT	60	3.6379788E-12	41.250004	1
TTACGGG	300	0.0	40.5	3
TACGGGA	175	0.0	39.857143	4
TACGGGT	40	3.4582263E-7	39.375	4
CGTTTTT	1735	0.0	39.034584	1
TAATGCG	110	0.0	38.863636	24
TCTTGCG	365	0.0	38.835617	1
TTGGGAC	2235	0.0	38.65772	5
CGCATCG	35	6.248296E-6	38.571426	21
GTATGCG	35	6.248296E-6	38.571426	1
TACGAAT	130	0.0	38.076927	12
CGGTAGT	220	0.0	37.840908	12
CGAATAT	125	0.0	37.800003	14
>>END_MODULE