FastQCFastQC Report
Sat 14 Jan 2017
SRR2933180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144679
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139250.6492813143598646No Hit
CTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGCT37380.17429181709710403TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC35520.16561919056418234TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC33610.1567134289094079TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC25910.12081062014408683TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCACG207.034656E-445.011
TCTATCG207.034656E-445.02
CGTTTTT69250.041.913361
TGGGCGA42150.037.419936
TCGTTTG6850.036.7883221
TTTGGGA91500.036.6885224
TTTGGGC60800.036.6365174
CGTTATT2150.036.6279071
TTGGGCG36400.036.3461535
GGGCGAT65400.036.3302737
TTGGGAC37900.036.0949865
CGGGTAT2200.035.7954566
TACGAAT1650.035.45454412
TTGGGAT44650.035.324755
TTGGGCA27650.035.1537065
TATGGGC6950.034.640294
CGTTTGG14850.034.5454522
GGCGATT32600.034.3711668
GTTGGGA29100.034.32994
TTATGGG19550.034.0664943
Produced by FastQC (version 0.11.5)