##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144679
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19407379845655	33.0	31.0	34.0	30.0	34.0
2	32.38543437036498	34.0	31.0	34.0	30.0	34.0
3	32.450744843400805	34.0	31.0	34.0	30.0	34.0
4	35.94572241347073	37.0	35.0	37.0	35.0	37.0
5	35.12936294895413	37.0	35.0	37.0	35.0	37.0
6	34.84104660883983	37.0	35.0	37.0	32.0	37.0
7	35.62479699759265	37.0	35.0	37.0	33.0	37.0
8	35.90568052375204	37.0	35.0	37.0	35.0	37.0
9	37.76986299581429	39.0	38.0	39.0	35.0	39.0
10	37.28960044836547	39.0	37.0	39.0	34.0	39.0
11	37.200486413118234	39.0	37.0	39.0	34.0	39.0
12	36.95948904241614	39.0	37.0	39.0	33.0	39.0
13	36.863817382461434	39.0	37.0	39.0	33.0	39.0
14	37.825892825919404	40.0	37.0	41.0	33.0	41.0
15	37.95532804676131	40.0	37.0	41.0	33.0	41.0
16	38.0068523075015	40.0	37.0	41.0	33.0	41.0
17	37.98415007560572	40.0	37.0	41.0	33.0	41.0
18	37.9185132133993	40.0	37.0	41.0	33.0	41.0
19	37.88824901069111	40.0	37.0	41.0	33.0	41.0
20	37.764420689529764	40.0	36.0	41.0	33.0	41.0
21	37.64213152644289	40.0	36.0	41.0	33.0	41.0
22	37.71229167628349	39.0	35.0	41.0	33.0	41.0
23	37.64716911015588	39.0	35.0	41.0	33.0	41.0
24	37.58165441075331	39.0	35.0	41.0	33.0	41.0
25	37.465943388264634	39.0	35.0	41.0	33.0	41.0
26	37.431781632589306	39.0	35.0	41.0	33.0	41.0
27	37.374795948484596	39.0	35.0	41.0	33.0	41.0
28	37.280821978487225	39.0	35.0	41.0	32.0	41.0
29	37.20059225646355	39.0	35.0	41.0	32.0	41.0
30	37.07951492974007	39.0	35.0	41.0	32.0	41.0
31	36.942390912579455	39.0	35.0	41.0	31.0	41.0
32	36.88604215362765	39.0	35.0	41.0	31.0	41.0
33	36.73161018502069	39.0	35.0	41.0	31.0	41.0
34	36.53942431478091	39.0	35.0	41.0	30.0	41.0
35	36.379639097505965	39.0	35.0	41.0	30.0	41.0
36	36.23220631152727	39.0	35.0	41.0	30.0	41.0
37	36.17394910846798	39.0	35.0	40.0	30.0	41.0
38	36.0903501176633	38.0	35.0	40.0	30.0	41.0
39	36.052682942295796	38.0	35.0	40.0	30.0	41.0
40	35.93218472321499	38.0	35.0	40.0	29.0	41.0
41	35.78356807708753	38.0	35.0	40.0	29.0	41.0
42	35.690947689607626	38.0	35.0	40.0	29.0	41.0
43	35.52887261916585	38.0	35.0	40.0	28.0	41.0
44	35.45978489088577	38.0	34.0	40.0	28.0	41.0
45	35.472444594272616	38.0	34.0	40.0	28.0	41.0
46	35.29907599225805	37.0	34.0	40.0	27.0	41.0
47	35.21436727827334	37.0	34.0	40.0	27.0	41.0
48	35.10419041730721	37.0	34.0	40.0	27.0	41.0
49	35.15829268622484	37.0	34.0	40.0	27.0	41.0
50	34.95668256181928	37.0	34.0	40.0	27.0	41.0
51	33.89101072934458	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	26.0
10	51.0
11	42.0
12	38.0
13	37.0
14	49.0
15	93.0
16	154.0
17	323.0
18	589.0
19	1059.0
20	1918.0
21	2985.0
22	4732.0
23	7014.0
24	10738.0
25	16074.0
26	22337.0
27	26799.0
28	29395.0
29	33586.0
30	40859.0
31	51106.0
32	66537.0
33	93915.0
34	154665.0
35	173483.0
36	190741.0
37	278724.0
38	428207.0
39	508202.0
40	194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.780155445173847	4.167197049068882	54.23995852059912	17.81268898515815
2	21.215249461574437	4.262689194979761	52.11796264149553	22.404098701950268
3	20.425294414688633	4.334494812510404	52.723507806995826	22.516702965805138
4	18.365638867168464	4.854479388290741	51.37468124600465	25.405200498536146
5	17.260298627440285	8.022878948318139	50.283655502758215	24.433166921483355
6	21.61391051994261	7.440087770710675	53.29921167689897	17.646790032447747
7	81.82366685177595	2.2867757832290985	11.089771476290858	4.799785888704091
8	84.73878841542253	2.7555638862505765	8.464483496131589	4.041164202195294
9	78.97456915463806	5.024155129975162	10.749720587556459	5.2515551278303185
10	40.8145927665632	24.642895277102074	17.839592778219956	16.702919178114765
11	32.39426506251052	22.3524359589477	25.362769906358945	19.89052907218283
12	27.44569233904002	20.41056027498754	31.63042114927222	20.513326236700223
13	25.870911217949168	20.2306265879416	33.72285549492488	20.175606699184353
14	22.080227390672448	21.877912731928646	34.75652999819553	21.285329879203367
15	20.215006534777466	20.9973147496665	36.87022626696116	21.917452448594872
16	24.44981276918364	20.347240775892335	32.79423167756107	22.408714777362952
17	24.659307989680507	20.703751004229538	30.108654954890685	24.52828605119927
18	25.322251022180943	20.230113690673523	31.918809295004053	22.52882599214148
19	25.536315691066125	22.526634521996066	29.638841057333053	22.298208729604756
20	26.044690137778193	24.795692035964358	28.235554131877077	20.924063694380372
21	26.300485993475014	23.814193172964345	29.24106591242792	20.64425492113272
22	24.127899792929387	21.98492175285905	29.959215341783086	23.92796311242848
23	22.790263717787138	23.25947146402795	29.554586024295475	24.395678793889434
24	23.140852314029278	23.398793012847143	30.00775407415282	23.452600598970754
25	23.914954172629095	23.534570907814174	27.65859133231593	24.891883587240795
26	21.927990156102613	23.56138144682724	28.323119683644965	26.187508713425178
27	21.766147754512446	22.492317032059344	30.51239835891525	25.229136854512962
28	20.57254255765082	23.363776117544862	29.766925493278944	26.29675583152537
29	21.95582648965183	23.97934609328482	28.52128453721979	25.543542879843557
30	24.805203948935947	23.112223321065763	29.062577663137468	23.019995066860822
31	24.46608560068896	24.516023143789816	27.65933736470586	23.35855389081536
32	24.834066077021316	24.019118945072897	27.07659281412277	24.07022216378302
33	25.524612307949113	23.832237831395748	26.770439772105753	23.872710088549383
34	22.586317113190365	23.80081121697	28.65072115687243	24.96215051296721
35	21.931813572101	25.45061522027306	27.996357496856174	24.62121371076977
36	24.532715618514473	26.122230879306414	26.327529667609934	23.017523834569182
37	22.66348483852362	27.5055614383318	26.710477418765233	23.120476304379352
38	23.705692087254082	25.690044990415817	28.328714926569432	22.27554799576067
39	24.35352796385846	23.468873430476076	27.232606837666616	24.944991767998847
40	25.46977892728935	21.962027883893114	28.34205025553941	24.226142933278126
41	22.40834176116799	22.605900463425996	28.043031148251092	26.94272662715493
42	22.495953939960245	23.720426226955176	28.522729974975274	25.2608898581093
43	22.716826154403527	22.96185116747075	29.42668809644707	24.894634581678655
44	23.170553728553315	22.851624881858775	28.63799197921927	25.339829410368637
45	22.622219921955686	22.400275285951885	27.78467080621389	27.19283398587854
46	22.84486396332505	23.093339376195694	28.588240944215894	25.47355571626337
47	20.98533160440327	24.697775284786207	29.728504825197614	24.588388285612904
48	20.84540390426726	24.97749080398512	29.673578190489113	24.50352710125851
49	23.3369655785318	22.933828325824052	29.358192997646732	24.371013097997416
50	22.217963620663046	22.777254777987753	28.86735031209799	26.13743128925121
51	21.468434203906504	22.581794291826423	28.259007525135466	27.69076397913161
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	392.0
1	1255.0
2	2118.0
3	12181.5
4	22245.0
5	14382.5
6	6520.0
7	6351.0
8	6182.0
9	6246.5
10	6311.0
11	6425.5
12	6540.0
13	6554.0
14	6568.0
15	6449.5
16	6331.0
17	6273.5
18	6216.0
19	6184.5
20	6153.0
21	6214.0
22	6275.0
23	7452.5
24	8630.0
25	9888.0
26	12182.0
27	13218.0
28	16843.0
29	20468.0
30	24361.0
31	28254.0
32	32164.5
33	36075.0
34	39714.0
35	43353.0
36	47805.5
37	52258.0
38	57379.5
39	62501.0
40	70103.5
41	77706.0
42	87004.0
43	96302.0
44	109360.0
45	122418.0
46	142557.5
47	162697.0
48	183797.5
49	204898.0
50	202579.5
51	200261.0
52	183209.0
53	166157.0
54	155390.0
55	144623.0
56	135889.0
57	127155.0
58	118566.5
59	109978.0
60	102422.5
61	94867.0
62	86354.0
63	77841.0
64	70475.5
65	63110.0
66	53644.5
67	44179.0
68	38068.5
69	31958.0
70	28322.0
71	24686.0
72	20183.5
73	15681.0
74	13014.0
75	8036.0
76	5725.0
77	4285.5
78	2846.0
79	2285.0
80	1724.0
81	1306.0
82	888.0
83	705.5
84	523.0
85	366.0
86	209.0
87	155.0
88	101.0
89	64.5
90	28.0
91	18.5
92	9.0
93	6.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2144679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.869879069941096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27715770703752	21.443725606611164
2	10.210778815300742	5.895678992152977
3	3.672115444946727	3.1804058647942486
4	1.8508543886842588	2.1373576950953725
5	1.107897939877748	1.599243977305373
6	0.7461647962664352	1.2925012460675531
7	0.5262097410075878	1.063412811181986
8	0.4303202175413287	0.9938634113415125
9	0.3653674402208097	0.9493302433741838
>10	6.235893367142896	46.30221908513644
>50	0.494454338922992	8.619411382458512
>100	0.07648159301316694	3.9649104369034793
>500	0.004479307132280565	0.8647000426116184
>1k	0.0016590026415853945	1.0262984664385597
>5k	0.0	0.0
>10k+	1.6590026415853944E-4	0.6669407385270182
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13925	0.6492813143598646	No Hit
CTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGCT	3738	0.17429181709710403	TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC	3552	0.16561919056418234	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC	3361	0.1567134289094079	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC	2591	0.12081062014408683	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7976214622328096E-4	0.0	0.0	0.18702099475026332	0.0
2	2.7976214622328096E-4	0.0	0.0	0.6806146747368721	0.0
3	2.7976214622328096E-4	0.0	0.0	1.041554470389275	0.0
4	2.7976214622328096E-4	0.0	0.0	1.4903395799557884	0.0
5	2.7976214622328096E-4	0.0	0.0	2.281693437572709	0.0
6	3.263891705938278E-4	0.0	0.0	3.1324501242377063	0.0
7	3.263891705938278E-4	0.0	0.0	3.877083703435339	0.0
8	3.263891705938278E-4	0.0	0.0	5.004245390568938	0.0
9	3.263891705938278E-4	0.0	0.0	5.79144944301688	0.0
10	3.263891705938278E-4	0.0	0.0	6.857156712030099	0.0
11	3.263891705938278E-4	0.0	0.0	8.374539966120803	0.0
12	3.263891705938278E-4	0.0	0.0	9.637945818465141	0.0
13	3.263891705938278E-4	0.0	0.0	10.068266626380918	0.0
14	3.263891705938278E-4	0.0	0.0	10.260696355958164	0.0
15	3.263891705938278E-4	0.0	0.0	10.475926700452609	0.0
16	3.263891705938278E-4	0.0	0.0	10.927695939578836	0.0
17	3.263891705938278E-4	0.0	0.0	11.562522876383833	0.0
18	3.263891705938278E-4	0.0	0.0	12.267896501061465	0.0
19	3.263891705938278E-4	0.0	0.0	12.752304657247075	0.0
20	3.263891705938278E-4	0.0	0.0	13.287023372728505	0.0
21	3.263891705938278E-4	0.0	0.0	13.994681721600296	0.0
22	3.263891705938278E-4	0.0	0.0	14.745749830161063	0.0
23	4.1964321933492146E-4	0.0	0.0	15.458490524689243	0.0
24	4.1964321933492146E-4	0.0	0.0	16.041747972540414	0.0
25	4.1964321933492146E-4	0.0	0.0	16.54886348959448	0.0
26	4.1964321933492146E-4	0.0	0.0	16.991260696822227	0.0
27	4.1964321933492146E-4	0.0	0.0	17.450630140920858	0.0
28	4.6627024370546826E-4	0.0	0.0	17.917786298089364	0.0
29	4.6627024370546826E-4	0.0	0.0	18.451805608205238	0.0
30	4.6627024370546826E-4	0.0	0.0	19.094559139153226	0.0
31	5.128972680760151E-4	0.0	0.0	19.601628029182923	0.0
32	5.128972680760151E-4	0.0	0.0	20.1104687461387	0.0
33	5.128972680760151E-4	0.0	0.0	20.628821376066067	0.0
34	5.128972680760151E-4	0.0	0.0	21.167969658862702	0.0
35	5.128972680760151E-4	0.0	0.0	21.79622218523145	0.0
36	5.128972680760151E-4	0.0	0.0	22.336676024710457	0.0
37	5.595242924465619E-4	0.0	0.0	22.8482677361041	0.0
38	6.061513168171088E-4	0.0	0.0	23.472090695157643	0.0
39	6.061513168171088E-4	0.0	0.0	24.415914922466254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCACG	20	7.034656E-4	45.0	11
TCTATCG	20	7.034656E-4	45.0	2
CGTTTTT	6925	0.0	41.91336	1
TGGGCGA	4215	0.0	37.41993	6
TCGTTTG	685	0.0	36.788322	1
TTTGGGA	9150	0.0	36.688522	4
TTTGGGC	6080	0.0	36.636517	4
CGTTATT	215	0.0	36.627907	1
TTGGGCG	3640	0.0	36.346153	5
GGGCGAT	6540	0.0	36.330273	7
TTGGGAC	3790	0.0	36.094986	5
CGGGTAT	220	0.0	35.795456	6
TACGAAT	165	0.0	35.454544	12
TTGGGAT	4465	0.0	35.32475	5
TTGGGCA	2765	0.0	35.153706	5
TATGGGC	695	0.0	34.64029	4
CGTTTGG	1485	0.0	34.545452	2
GGCGATT	3260	0.0	34.371166	8
GTTGGGA	2910	0.0	34.3299	4
TTATGGG	1955	0.0	34.066494	3
>>END_MODULE