FastQCFastQC Report
Sat 14 Jan 2017
SRR2933178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672504
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59510.35581379775474375No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT47650.28490215867944113TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC46780.27970037739820053TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC38140.228041308122432TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC31120.18606831433587004TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG207.0342096E-445.020
TATCGCG453.8562575E-1045.01
GCTACGA2000.040.50000410
TGTAGCG403.4594814E-739.3751
CGAATAT1950.039.2307714
CGTTTTT36650.038.7380641
CTACGAA2100.038.5714311
TTTGGGA94700.038.2761344
AACACGT4350.037.7586241
TATACGG301.140144E-437.5000042
TGGGCGA29900.037.4749156
TTACGGG3850.037.40263
TTGGGAT53750.037.1720925
TACGGGA3400.037.0588234
CACGACC4800.037.03125427
TCAAGCG5000.036.89999817
TAACGGG552.750312E-936.818183
GCGATCG552.750312E-936.818189
TACGAAT2150.036.62790712
AAACACG4550.036.59340740
Produced by FastQC (version 0.11.5)