##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933178.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1672504
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.221468528625344	33.0	31.0	34.0	30.0	34.0
2	32.407079444952004	34.0	31.0	34.0	30.0	34.0
3	32.47269005036759	34.0	31.0	34.0	30.0	34.0
4	35.95662910223234	37.0	35.0	37.0	35.0	37.0
5	35.185738270282165	37.0	35.0	37.0	35.0	37.0
6	34.88329355266116	37.0	35.0	37.0	32.0	37.0
7	35.67376101940563	37.0	35.0	37.0	35.0	37.0
8	35.951641371261296	37.0	35.0	37.0	35.0	37.0
9	37.81156876156948	39.0	38.0	39.0	35.0	39.0
10	37.28877419725155	39.0	37.0	39.0	34.0	39.0
11	37.21804671319172	39.0	37.0	39.0	34.0	39.0
12	37.08977855957295	39.0	37.0	39.0	33.0	39.0
13	37.08320637798176	39.0	37.0	39.0	33.0	39.0
14	38.25134947360365	40.0	38.0	41.0	33.0	41.0
15	38.32257202374404	40.0	38.0	41.0	34.0	41.0
16	38.30155443574425	40.0	37.0	41.0	34.0	41.0
17	38.237475067324205	40.0	37.0	41.0	34.0	41.0
18	38.17613709742996	40.0	37.0	41.0	34.0	41.0
19	38.180375652315334	40.0	37.0	41.0	34.0	41.0
20	38.10315789977184	40.0	37.0	41.0	34.0	41.0
21	37.98553725432047	40.0	37.0	41.0	33.0	41.0
22	38.0238116022443	40.0	37.0	41.0	34.0	41.0
23	37.945913432792985	40.0	36.0	41.0	33.0	41.0
24	37.88392374547385	40.0	36.0	41.0	33.0	41.0
25	37.7898779315326	40.0	36.0	41.0	33.0	41.0
26	37.778304267134786	40.0	36.0	41.0	33.0	41.0
27	37.75038624720778	40.0	36.0	41.0	33.0	41.0
28	37.66746208080818	40.0	36.0	41.0	33.0	41.0
29	37.59034776598442	40.0	36.0	41.0	33.0	41.0
30	37.477528902770935	39.0	36.0	41.0	33.0	41.0
31	37.407704256611645	39.0	36.0	41.0	33.0	41.0
32	37.348944456934035	39.0	35.0	41.0	32.0	41.0
33	37.23547626791924	39.0	35.0	41.0	32.0	41.0
34	37.104942050960716	39.0	35.0	41.0	32.0	41.0
35	36.95115886120452	39.0	35.0	41.0	31.0	41.0
36	36.796367602110365	39.0	35.0	41.0	31.0	41.0
37	36.78708032985272	39.0	35.0	41.0	31.0	41.0
38	36.67941481754304	39.0	35.0	41.0	31.0	41.0
39	36.61162663886006	39.0	35.0	41.0	31.0	41.0
40	36.50382241238287	39.0	35.0	41.0	30.0	41.0
41	36.37670522761081	39.0	35.0	40.0	30.0	41.0
42	36.307052180443215	39.0	35.0	40.0	30.0	41.0
43	36.232270894419386	39.0	35.0	40.0	30.0	41.0
44	36.170291371500454	38.0	35.0	40.0	30.0	41.0
45	36.14259397884848	38.0	35.0	40.0	30.0	41.0
46	35.921506914183766	38.0	35.0	40.0	29.0	41.0
47	35.832387246906436	38.0	35.0	40.0	29.0	41.0
48	35.744579983067304	38.0	35.0	40.0	29.0	41.0
49	35.77739903761067	38.0	35.0	40.0	29.0	41.0
50	35.589448515519244	38.0	34.0	40.0	28.0	41.0
51	34.467866743517504	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	17.0
10	31.0
11	30.0
12	22.0
13	20.0
14	34.0
15	50.0
16	97.0
17	181.0
18	290.0
19	543.0
20	1010.0
21	1632.0
22	2451.0
23	3807.0
24	5995.0
25	9089.0
26	12825.0
27	16102.0
28	18555.0
29	21974.0
30	27259.0
31	35109.0
32	45851.0
33	63471.0
34	104799.0
35	132726.0
36	149024.0
37	218962.0
38	355834.0
39	444541.0
40	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.713665258797587	4.152695598934293	54.998553067735564	17.13508607453256
2	20.524554799271034	4.271499500150672	53.42241333951967	21.781532361058627
3	20.42446535254923	4.461573783978992	52.72166763128817	22.3922932321836
4	18.586861376714197	4.5543687787891685	51.54307553225582	25.315694312240804
5	17.064533178993894	7.651820264704898	50.71790560740064	24.565740948900572
6	21.653819662015756	7.3506550656979	53.28340021907272	17.712125053213626
7	83.16129587731928	2.0524315636913273	10.021979020677978	4.764293538311418
8	85.82323270975735	2.4104576132553346	7.476334884699827	4.2899747922874925
9	79.93116907343719	5.19293227400353	9.634117466983637	5.24178118557564
10	37.49718984229634	29.518554215714882	17.26614704658404	15.718108895404734
11	29.420138905497385	24.75713062569656	26.35120753074432	19.47152293806173
12	25.503556344259863	22.386732707365724	31.22659198423442	20.883118964139996
13	23.897162577787554	22.199109837704423	32.86048942184892	21.043238162659105
14	21.165569708652416	24.651062120030804	31.826052433955315	22.357315737361468
15	20.053464745076845	23.910974203948093	34.044103930394186	21.991457120580876
16	22.229483457139715	22.884011039734435	32.016007136604756	22.870498366521097
17	21.881263064243793	22.275283048650408	30.993229313651867	24.850224573453936
18	22.459139111177016	22.469602464328933	31.373617043666265	23.69764138082779
19	23.43886770973343	24.566757388921044	29.952035989151593	22.04233891219393
20	26.031865992547704	24.619074154680646	29.04315923908104	20.30590061369061
21	25.479759689662924	25.16125521971846	28.588392015803848	20.77059307481477
22	23.56813496410173	23.816804025580808	28.34241353084955	24.272647479467913
23	22.6928605252962	24.982003032578696	28.003520469906203	24.3216159722189
24	22.149184695522404	23.966579452126872	29.907671371787455	23.976564480563276
25	21.73405863304363	24.9076235393159	28.609498093876006	24.748819733764464
26	21.34069634512085	25.26905765247796	28.900259730320528	24.489986272080667
27	21.934596270023867	23.934830649134472	29.718613527979603	24.411959552862054
28	20.3551680593888	24.617698971123538	30.608177917661184	24.418955051826483
29	22.16287674050406	23.193726293031286	30.091766596671814	24.551630369792836
30	23.743082228801846	23.162216652396648	30.002319874870253	23.092381243931257
31	23.953903847165687	24.835157344915167	27.399037610672384	23.811901197246762
32	23.590257482194364	24.74003051711685	28.46803355926204	23.201678441426747
33	23.82397889631355	23.52957003391322	29.034429813022868	23.612021256750356
34	22.51946781592152	22.919347278093205	30.34802906300971	24.21315584297556
35	22.638152135959018	23.09734386285474	30.320644973046402	23.943859028139844
36	23.074623438867707	24.807892836130733	29.57804585220723	22.539437872794323
37	23.64161759852293	25.072406403811293	28.696433610921112	22.589542386744665
38	22.13896050472824	25.402031923391515	29.409735342934905	23.04927222894534
39	23.270258247513908	23.479465519962883	30.20393374246041	23.046342490062806
40	23.609569842583337	22.94643241510932	31.502585345087365	21.941412397219974
41	20.967184532892	23.66882231671793	31.185157105752808	24.17883604463726
42	21.641443010001773	23.875398803231562	30.474605740853235	24.008552445913434
43	22.362397937463825	22.77770337171092	30.35622037376293	24.50367831706232
44	22.25794377771294	23.33088590520561	29.174818117026923	25.23635220005453
45	23.341708001894165	22.266254669645036	28.624804484772532	25.767232843688266
46	23.241140230456846	23.260930915561335	29.866894189789683	23.631034664192132
47	21.269724915456106	23.509599977040413	31.693975021883357	23.526700085620124
48	21.710501140804446	22.62888459459589	31.605963274228344	24.054650990371325
49	22.720902311743348	22.033968229672396	31.566322113429923	23.678807345154333
50	22.247659796329337	21.550202570517023	30.879686984306165	25.32245064884748
51	21.319171732922612	21.44096516361097	29.973979135475908	27.26588396799051
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	365.0
1	1082.0
2	1799.0
3	6260.5
4	10722.0
5	7296.5
6	3871.0
7	3797.0
8	3723.0
9	3840.0
10	3957.0
11	3995.5
12	4034.0
13	4010.5
14	3987.0
15	3925.5
16	3864.0
17	3745.0
18	3626.0
19	4340.5
20	5055.0
21	5222.0
22	5389.0
23	6419.0
24	7449.0
25	8495.5
26	12229.5
27	14917.0
28	17655.0
29	20393.0
30	23613.0
31	26833.0
32	29854.0
33	32875.0
34	37382.5
35	41890.0
36	44818.0
37	47746.0
38	53818.0
39	59890.0
40	67144.0
41	74398.0
42	81826.0
43	89254.0
44	99637.0
45	110020.0
46	125145.5
47	140271.0
48	154982.0
49	169693.0
50	167376.0
51	165059.0
52	145169.5
53	125280.0
54	113031.0
55	100782.0
56	92274.0
57	83766.0
58	79526.0
59	75286.0
60	69350.0
61	63414.0
62	56432.0
63	49450.0
64	43534.5
65	37619.0
66	31719.5
67	25820.0
68	21295.5
69	16771.0
70	14405.5
71	12040.0
72	10400.0
73	8760.0
74	7153.0
75	4501.0
76	3456.0
77	2661.5
78	1867.0
79	1402.0
80	937.0
81	754.0
82	571.0
83	421.5
84	272.0
85	207.5
86	143.0
87	95.5
88	48.0
89	41.0
90	34.0
91	24.5
92	15.0
93	9.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1672504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.065595266361086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.13802449765991	24.414110779222483
2	10.144420090000574	6.505737376358015
3	3.592263057408044	3.455641598674416
4	1.7160734282153614	2.2010766398604216
5	0.9918236159020029	1.59017073215662
6	0.6731354101907563	1.2950692573579645
7	0.47428925137280975	1.064585702159414
8	0.36364263091604476	0.9328333939638894
9	0.3117919080213086	0.8998013816943987
>10	4.980450509135616	40.99022557859131
>50	0.541766204619114	10.607268994896705
>100	0.06751043939208885	3.7543487742270862
>500	0.002959358256330159	0.6177572324626617
>1k	0.0016646390191857143	1.3184277340288948
>5k	1.8495989102063494E-4	0.35294482434574687
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5951	0.35581379775474375	No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	4765	0.28490215867944113	TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	4678	0.27970037739820053	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	3814	0.228041308122432	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	3112	0.18606831433587004	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.979058943954693E-5	0.0	0.0	0.3080411167925458	0.0
2	5.979058943954693E-5	0.0	0.0	1.0896237019463033	0.0
3	5.979058943954693E-5	0.0	0.0	1.5740470575855126	0.0
4	5.979058943954693E-5	0.0	0.0	2.115211682602852	0.0
5	5.979058943954693E-5	0.0	0.0	2.9926983732176424	0.0
6	5.979058943954693E-5	0.0	0.0	3.7713512194888623	0.0
7	5.979058943954693E-5	0.0	0.0	4.456969908592147	0.0
8	5.979058943954693E-5	0.0	0.0	5.533320099682871	0.0
9	5.979058943954693E-5	0.0	0.0	6.155620554569675	0.0
10	5.979058943954693E-5	0.0	0.0	7.139175750850222	0.0
11	5.979058943954693E-5	0.0	0.0	8.739949201915213	0.0
12	1.1958117887909386E-4	0.0	0.0	10.146403237301675	0.0
13	1.1958117887909386E-4	0.0	0.0	10.614802714971086	0.0
14	1.1958117887909386E-4	0.0	0.0	10.78442861721108	0.0
15	1.1958117887909386E-4	0.0	0.0	11.032021448080243	0.0
16	1.1958117887909386E-4	0.0	0.0	11.59339529232815	0.0
17	1.1958117887909386E-4	0.0	0.0	12.428968779745817	0.0
18	1.1958117887909386E-4	0.0	0.0	13.315065315239904	1.1958117887909386E-4
19	1.1958117887909386E-4	0.0	0.0	13.920684195673074	1.7937176831864078E-4
20	1.7937176831864078E-4	0.0	0.0	14.545974180031857	1.7937176831864078E-4
21	1.7937176831864078E-4	0.0	0.0	15.392309973548643	1.7937176831864078E-4
22	1.7937176831864078E-4	0.0	0.0	16.300349655367043	1.7937176831864078E-4
23	1.7937176831864078E-4	0.0	0.0	17.17705906831912	1.7937176831864078E-4
24	1.7937176831864078E-4	0.0	0.0	17.898073786370617	1.7937176831864078E-4
25	1.7937176831864078E-4	0.0	0.0	18.515172459976178	1.7937176831864078E-4
26	1.7937176831864078E-4	0.0	0.0	19.067398344039834	1.7937176831864078E-4
27	1.7937176831864078E-4	0.0	0.0	19.62488579997417	1.7937176831864078E-4
28	1.7937176831864078E-4	0.0	0.0	20.183868020644496	1.7937176831864078E-4
29	1.7937176831864078E-4	0.0	0.0	20.786497371605687	1.7937176831864078E-4
30	1.7937176831864078E-4	0.0	0.0	21.45812506278012	1.7937176831864078E-4
31	1.7937176831864078E-4	0.0	0.0	22.078392637625978	1.7937176831864078E-4
32	2.3916235775818772E-4	0.0	0.0	22.672591515476196	1.7937176831864078E-4
33	2.3916235775818772E-4	0.0	0.0	23.280183485360872	1.7937176831864078E-4
34	2.3916235775818772E-4	0.0	0.0	23.868164141909375	1.7937176831864078E-4
35	2.3916235775818772E-4	0.0	0.0	24.50236292409465	1.7937176831864078E-4
36	2.3916235775818772E-4	0.0	0.0	25.09381143483065	1.7937176831864078E-4
37	2.3916235775818772E-4	0.0	0.0	25.69147816686836	1.7937176831864078E-4
38	2.9895294719773464E-4	0.0	0.0	26.29608060728106	1.7937176831864078E-4
39	2.9895294719773464E-4	0.0	0.0	26.878739901369443	1.7937176831864078E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	20	7.0342096E-4	45.0	20
TATCGCG	45	3.8562575E-10	45.0	1
GCTACGA	200	0.0	40.500004	10
TGTAGCG	40	3.4594814E-7	39.375	1
CGAATAT	195	0.0	39.23077	14
CGTTTTT	3665	0.0	38.738064	1
CTACGAA	210	0.0	38.57143	11
TTTGGGA	9470	0.0	38.276134	4
AACACGT	435	0.0	37.75862	41
TATACGG	30	1.140144E-4	37.500004	2
TGGGCGA	2990	0.0	37.474915	6
TTACGGG	385	0.0	37.4026	3
TTGGGAT	5375	0.0	37.172092	5
TACGGGA	340	0.0	37.058823	4
CACGACC	480	0.0	37.031254	27
TCAAGCG	500	0.0	36.899998	17
TAACGGG	55	2.750312E-9	36.81818	3
GCGATCG	55	2.750312E-9	36.81818	9
TACGAAT	215	0.0	36.627907	12
AAACACG	455	0.0	36.593407	40
>>END_MODULE