SRR2933177.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	748564
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2794	0.37324797879673616	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2747	0.36696929053494426	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	2679	0.3578852309221389	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2247	0.3001747345584345	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1789	0.2389909212839517	No Hit
TCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	1006	0.1343906466247375	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	903	0.1206309680935765	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATACG	20	7.031707E-4	45.000004	30
CGAATCT	20	7.031707E-4	45.000004	29
TACGTAA	20	7.031707E-4	45.000004	27
GTCGAAT	25	3.889497E-5	45.0	30
TTCGTAC	50	2.1827873E-11	45.0	33
CGAATAT	25	3.889497E-5	45.0	14
GCCGATT	160	0.0	43.593754	9
TTCACGG	115	0.0	43.04348	2
CTACGGG	60	3.6379788E-12	41.249996	3
TCGTACA	55	6.002665E-11	40.909092	34
GTTCACG	55	6.002665E-11	40.909092	1
CGTTAGG	50	1.0804797E-9	40.5	2
TAATGCG	95	0.0	40.263157	24
AGTCACG	45	1.9266736E-8	40.0	11
CGTTGAT	45	1.9266736E-8	40.0	25
TTCGTCG	90	0.0	40.0	1
CGTTTTT	1215	0.0	39.629627	1
TACGCGC	80	0.0	39.375004	34
GCGATAG	40	3.4560617E-7	39.375004	9
TTACACG	110	0.0	38.863636	34