##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	748564
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.097813947772	33.0	31.0	34.0	30.0	34.0
2	32.28696277138628	34.0	31.0	34.0	30.0	34.0
3	32.35962856883312	34.0	31.0	34.0	30.0	34.0
4	35.85861863514676	37.0	35.0	37.0	35.0	37.0
5	35.06305406084183	37.0	35.0	37.0	33.0	37.0
6	34.76209248641399	37.0	35.0	37.0	32.0	37.0
7	35.58722701064973	37.0	35.0	37.0	33.0	37.0
8	35.86922694652695	37.0	35.0	37.0	35.0	37.0
9	37.71535366381499	39.0	38.0	39.0	35.0	39.0
10	37.148006048914986	39.0	37.0	39.0	34.0	39.0
11	37.08247658182867	39.0	37.0	39.0	33.0	39.0
12	36.95011648970562	39.0	37.0	39.0	33.0	39.0
13	36.854806001891625	39.0	37.0	39.0	33.0	39.0
14	37.93695128272265	40.0	37.0	41.0	33.0	41.0
15	38.05437477623824	40.0	37.0	41.0	33.0	41.0
16	38.04640752160136	40.0	37.0	41.0	33.0	41.0
17	38.02275556933008	40.0	37.0	41.0	33.0	41.0
18	37.968176401750554	40.0	37.0	41.0	33.0	41.0
19	37.99363982237992	40.0	37.0	41.0	34.0	41.0
20	37.87346572904922	40.0	36.0	41.0	33.0	41.0
21	37.75613841969424	39.0	36.0	41.0	33.0	41.0
22	37.791550221490745	39.0	36.0	41.0	33.0	41.0
23	37.72213063946436	39.0	36.0	41.0	33.0	41.0
24	37.6728776697784	39.0	36.0	41.0	33.0	41.0
25	37.57330168161974	39.0	36.0	41.0	33.0	41.0
26	37.555997883948464	39.0	36.0	41.0	33.0	41.0
27	37.50818233310712	39.0	36.0	41.0	33.0	41.0
28	37.431479472697056	39.0	36.0	41.0	33.0	41.0
29	37.39142812104242	39.0	36.0	41.0	33.0	41.0
30	37.290480172704	39.0	36.0	41.0	32.0	41.0
31	37.21464029795715	39.0	35.0	41.0	32.0	41.0
32	37.21118167584869	39.0	35.0	41.0	32.0	41.0
33	37.11041407281141	39.0	35.0	41.0	32.0	41.0
34	37.025918959501126	39.0	35.0	41.0	31.0	41.0
35	36.91781731421762	39.0	35.0	41.0	31.0	41.0
36	36.77909704447449	39.0	35.0	41.0	31.0	41.0
37	36.72125295899883	39.0	35.0	41.0	31.0	41.0
38	36.60414473578746	39.0	35.0	40.0	31.0	41.0
39	36.57111082018371	39.0	35.0	40.0	31.0	41.0
40	36.481468518389875	39.0	35.0	40.0	30.0	41.0
41	36.39945415488856	39.0	35.0	40.0	30.0	41.0
42	36.30583356934076	39.0	35.0	40.0	30.0	41.0
43	36.128276272970645	38.0	35.0	40.0	30.0	41.0
44	36.05831966271421	38.0	35.0	40.0	30.0	41.0
45	36.05691163347422	38.0	35.0	40.0	30.0	41.0
46	35.803245948242235	38.0	35.0	40.0	29.0	41.0
47	35.713762617491625	38.0	35.0	40.0	29.0	41.0
48	35.643266307222895	38.0	34.0	40.0	28.0	41.0
49	35.724483143725855	38.0	35.0	40.0	29.0	41.0
50	35.53217226583165	38.0	34.0	40.0	28.0	41.0
51	34.361127438669236	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	10.0
10	21.0
11	18.0
12	16.0
13	13.0
14	23.0
15	25.0
16	34.0
17	84.0
18	119.0
19	268.0
20	455.0
21	792.0
22	1251.0
23	1778.0
24	2717.0
25	4112.0
26	5736.0
27	7156.0
28	8539.0
29	10328.0
30	12951.0
31	16913.0
32	21982.0
33	30544.0
34	50056.0
35	65075.0
36	68470.0
37	100034.0
38	159980.0
39	179001.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.66429056166206	4.427944704794781	54.68964577511074	16.218118958432413
2	20.30167093261231	4.128437915796111	53.72406367391432	21.84582747767726
3	20.80610341934691	4.552316168023041	51.83431209622691	22.80726831640314
4	19.208644818612704	4.56727814856178	50.88342479734532	25.3406522354802
5	17.181563633837587	7.994106048380632	50.08322601674673	24.74110430103505
6	20.6574187377432	7.725992700690923	53.37232354214202	18.244265019423857
7	82.44705863493303	2.0408408633062773	10.367984567785788	5.144115933974917
8	85.0503096595615	2.5609032761393817	7.48780331407869	4.900983750220423
9	78.73956001090086	5.3221902202082925	10.17387958811805	5.7643701807727865
10	37.10357431027941	27.671381471724526	17.932334443013556	17.2927097749825
11	28.53904275385939	24.376005258067444	26.168637551365016	20.91631443670815
12	25.65004461876339	21.281814247011614	32.40123756953313	20.666903564691864
13	22.74862269625576	23.897355469939775	33.930832901395206	19.423188932409253
14	20.244361203584464	25.819836380055676	33.12275236319139	20.813050053168467
15	18.23598249448277	23.581684398394792	37.11933782548987	21.062995281632567
16	20.24730016404743	22.271976744807393	34.20054931842835	23.28017377271683
17	21.144083872588048	22.106326245985645	30.135566230809925	26.61402365061638
18	22.03873015533742	22.08735659208832	32.19283855488642	23.68107469768784
19	24.113101885743905	24.144228148828958	29.23677868559001	22.50589127983713
20	25.564280408889555	24.336596470041304	28.591944042192786	21.507179078876355
21	24.476197092032212	25.26009800097253	28.68358617299256	21.580118734002703
22	22.642686530477018	23.41416365200571	28.982157838207556	24.960991979309718
23	21.309467193185885	24.911296829663197	27.69262214052506	26.08661383662586
24	22.580033236971055	23.405213181504855	29.872796447598336	24.141957133925757
25	20.916047258484245	24.761276256939954	28.558012407756717	25.764664076819084
26	19.631320768832058	26.791429991289988	27.96835006759609	25.608899172281863
27	19.928690132039478	24.92438856263459	30.42304999973282	24.72387130559311
28	18.74482342191182	24.426368353273734	32.11575763728953	24.713050587524915
29	19.30055412763638	23.40681625084829	31.440731854590926	25.851897766924402
30	21.4936865785691	25.005209975366167	29.828044095094075	23.673059350970657
31	22.271308799247628	26.18613772503086	27.26553774961126	24.27701572611026
32	22.856963466049663	26.4424952308687	25.541837438081444	25.158703865000188
33	21.700616112984328	25.60008229089296	26.566599515873058	26.132702080249654
34	20.115581299661752	24.91503732479788	27.461913744182194	27.507467631358175
35	19.721894186736204	24.19432406581134	28.39984289920434	27.68393884824811
36	21.639833067045704	24.82406313955787	28.292036485858258	25.244067307538167
37	20.163673379964838	27.127139429627928	29.217541853468777	23.491645336938458
38	20.498581283631058	27.888998135096	27.930143581577525	23.682276999695418
39	20.482417001084745	26.365681491495717	28.09873304086224	25.053168466557302
40	21.987565525459413	24.989312871043758	27.120059206694417	25.90306239680241
41	20.699499308008402	23.38904889895854	26.550969589774553	29.360482203258503
42	21.95737438615803	23.115591986790708	26.899102815524124	28.027930811527135
43	21.58038591222661	23.138435724934673	28.55854676420453	26.722631598634184
44	20.401061231905356	24.039761463281696	29.0283796709433	26.530797633869653
45	20.458370960933202	24.223847259552958	27.339412528521272	27.978369250992568
46	21.69553972673011	24.22358008132905	28.91696635157448	25.16391384036635
47	19.625977204353937	24.72974922651904	29.918884691222125	25.725388877904898
48	20.384095414687323	23.501664520334934	29.884552289450202	26.229687775527545
49	20.424172148273225	22.422264495754536	31.557221560213954	25.596341795758278
50	20.79635141417434	22.780416904900584	28.74784253584196	27.675389145083116
51	20.172089494017882	23.03637364340257	27.140631929935182	29.650904932644366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	424.0
2	668.0
3	1956.5
4	3245.0
5	2248.5
6	1252.0
7	1295.0
8	1338.0
9	1417.5
10	1497.0
11	1548.0
12	1599.0
13	1649.0
14	1699.0
15	1709.5
16	1720.0
17	1769.0
18	1818.0
19	1850.5
20	1883.0
21	2136.0
22	2389.0
23	2717.0
24	3045.0
25	3535.5
26	4629.5
27	5233.0
28	6470.5
29	7708.0
30	8287.5
31	8867.0
32	10655.0
33	12443.0
34	14879.0
35	17315.0
36	18844.0
37	20373.0
38	22861.0
39	25349.0
40	29473.0
41	33597.0
42	39333.0
43	45069.0
44	50495.0
45	55921.0
46	63058.0
47	70195.0
48	77943.5
49	85692.0
50	82741.0
51	79790.0
52	68490.0
53	57190.0
54	49777.5
55	42365.0
56	38813.0
57	35261.0
58	32196.5
59	29132.0
60	26746.0
61	24360.0
62	22527.5
63	20695.0
64	17941.5
65	15188.0
66	12726.5
67	10265.0
68	8827.5
69	7390.0
70	6101.5
71	4813.0
72	4014.5
73	3216.0
74	2556.0
75	1639.0
76	1382.0
77	1112.0
78	842.0
79	607.0
80	372.0
81	262.5
82	153.0
83	109.0
84	65.0
85	56.5
86	48.0
87	28.5
88	9.0
89	7.0
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	748564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.20623198843907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69747854140337	25.551192799386936
2	10.992866120803708	7.520490574921279
3	3.8834945672949823	3.9851914828420485
4	1.898615917055488	2.5977798606297204
5	1.065119227725255	1.8216857699458573
6	0.7053108370461507	1.447561566957647
7	0.5179934224060224	1.240302222271414
8	0.3929764173693218	1.0753793998816545
9	0.3236051612133498	0.9962381895408063
>10	5.2344851273118245	43.22356258511621
>50	0.2243428392282914	4.8378736422028
>100	0.059464366399466506	3.4990976139937673
>500	0.0019306612467359257	0.45198218462504425
>1k	0.002316793496083111	1.7516621076847858
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2794	0.37324797879673616	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2747	0.36696929053494426	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	2679	0.3578852309221389	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2247	0.3001747345584345	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1789	0.2389909212839517	No Hit
TCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	1006	0.1343906466247375	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	903	0.1206309680935765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0076733585905813E-4	0.0	0.0	0.38981302867891054	0.0
2	4.0076733585905813E-4	0.0	0.0	1.5553780304690046	0.0
3	4.0076733585905813E-4	0.0	0.0	2.232006882511048	0.0
4	4.0076733585905813E-4	0.0	0.0	3.0167093261230837	0.0
5	4.0076733585905813E-4	0.0	0.0	4.227561036865251	0.0
6	4.0076733585905813E-4	0.0	0.0	5.060088382556468	0.0
7	4.0076733585905813E-4	0.0	0.0	5.854275653117169	0.0
8	4.0076733585905813E-4	0.0	0.0	7.100929245862745	0.0
9	4.0076733585905813E-4	0.0	0.0	7.821508915737332	0.0
10	5.343564478120775E-4	0.0	0.0	9.031024735359969	0.0
11	5.343564478120775E-4	0.0	0.0	11.051159286313528	0.0
12	5.343564478120775E-4	0.0	0.0	12.816272222548774	0.0
13	5.343564478120775E-4	0.0	0.0	13.405667384485495	0.0
14	5.343564478120775E-4	0.0	0.0	13.597234171026125	0.0
15	5.343564478120775E-4	0.0	0.0	13.890595860874955	0.0
16	5.343564478120775E-4	0.0	0.0	14.630946719318588	0.0
17	5.343564478120775E-4	0.0	0.0	15.800920161803132	0.0
18	5.343564478120775E-4	0.0	0.0	17.04396684852598	0.0
19	5.343564478120775E-4	0.0	0.0	17.822524192988176	0.0
20	5.343564478120775E-4	0.0	0.0	18.646368246402446	0.0
21	5.343564478120775E-4	0.0	0.0	19.743669211984546	0.0
22	5.343564478120775E-4	0.0	0.0	20.81812643942268	0.0
23	5.343564478120775E-4	0.0	0.0	21.87508349319497	0.0
24	5.343564478120775E-4	0.0	0.0	22.69104578900401	0.0
25	5.343564478120775E-4	0.0	0.0	23.343361422670608	0.0
26	5.343564478120775E-4	0.0	0.0	23.951058292944893	0.0
27	5.343564478120775E-4	0.0	0.0	24.52308687032772	0.0
28	5.343564478120775E-4	0.0	0.0	25.208666192870616	0.0
29	5.343564478120775E-4	0.0	0.0	25.913215169310842	0.0
30	5.343564478120775E-4	0.0	0.0	26.711543702342084	0.0
31	5.343564478120775E-4	0.0	0.0	27.406340673609737	0.0
32	5.343564478120775E-4	0.0	0.0	28.038350762259473	0.0
33	5.343564478120775E-4	0.0	0.0	28.694006123724893	0.0
34	5.343564478120775E-4	0.0	0.0	29.32534826681486	0.0
35	5.343564478120775E-4	0.0	0.0	30.002377886192765	0.0
36	5.343564478120775E-4	0.0	0.0	30.64694535136608	0.0
37	5.343564478120775E-4	0.0	0.0	31.307677099085716	0.0
38	5.343564478120775E-4	0.0	0.0	31.93781694016811	0.0
39	5.343564478120775E-4	0.0	0.0	32.60162123746266	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATACG	20	7.031707E-4	45.000004	30
CGAATCT	20	7.031707E-4	45.000004	29
TACGTAA	20	7.031707E-4	45.000004	27
GTCGAAT	25	3.889497E-5	45.0	30
TTCGTAC	50	2.1827873E-11	45.0	33
CGAATAT	25	3.889497E-5	45.0	14
GCCGATT	160	0.0	43.593754	9
TTCACGG	115	0.0	43.04348	2
CTACGGG	60	3.6379788E-12	41.249996	3
TCGTACA	55	6.002665E-11	40.909092	34
GTTCACG	55	6.002665E-11	40.909092	1
CGTTAGG	50	1.0804797E-9	40.5	2
TAATGCG	95	0.0	40.263157	24
AGTCACG	45	1.9266736E-8	40.0	11
CGTTGAT	45	1.9266736E-8	40.0	25
TTCGTCG	90	0.0	40.0	1
CGTTTTT	1215	0.0	39.629627	1
TACGCGC	80	0.0	39.375004	34
GCGATAG	40	3.4560617E-7	39.375004	9
TTACACG	110	0.0	38.863636	34
>>END_MODULE