FastQCFastQC Report
Sat 14 Jan 2017
SRR2933176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077698
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387281.8639860075910935No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTT50050.24089160214814664No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT43990.21172470686307635No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC41000.19733377998149876No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG39650.1908362042991811No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC36800.17711910008095497No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT33780.16258378262865922No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC29110.14010698378686412No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT23350.11238399420897552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT214050.043.7386131
CCATACG1250.039.6000022
GTTTTTT245200.038.650082
CGTTATT5800.038.4051741
GAATCGT301.1402393E-437.4999969
TTGGGAC32400.036.527785
CGTTTTC6900.036.5217361
TGGGCGA34200.035.9210556
CGTTTAT2700.035.8333321
TTTGGGA86850.035.725394
GGAACAT17900.035.4469268
CACGACC15900.035.37735727
TATTGCG1150.035.217391
CGACCAA15950.035.12539329
CTTGGGA44950.035.0389334
TGGGAAC30550.034.7626846
TTACGGG5250.034.7142873
TTGGGAG48050.034.698235
ACAACGA2400.034.687513
GACACGA16300.034.6472425
Produced by FastQC (version 0.11.5)