##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933176.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2077698
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1427074579655	33.0	31.0	34.0	30.0	34.0
2	32.387650178226096	34.0	31.0	34.0	30.0	34.0
3	32.3621984523256	34.0	31.0	34.0	30.0	34.0
4	35.92029592366167	37.0	35.0	37.0	35.0	37.0
5	35.196628672694494	37.0	35.0	37.0	35.0	37.0
6	34.91302826493552	37.0	35.0	37.0	32.0	37.0
7	35.64740640843857	37.0	35.0	37.0	33.0	37.0
8	35.90798855271555	37.0	35.0	37.0	35.0	37.0
9	37.76211460953421	39.0	38.0	39.0	35.0	39.0
10	37.361453878282596	39.0	37.0	39.0	34.0	39.0
11	37.29756249464552	39.0	37.0	39.0	34.0	39.0
12	37.095320397863404	39.0	37.0	39.0	33.0	39.0
13	37.03482411784581	39.0	37.0	39.0	33.0	39.0
14	38.08853019062443	40.0	37.0	41.0	33.0	41.0
15	38.174706814946155	40.0	37.0	41.0	33.0	41.0
16	38.16092136585779	40.0	37.0	41.0	33.0	41.0
17	38.07762100170477	40.0	37.0	41.0	33.0	41.0
18	37.919017104507006	39.0	37.0	41.0	33.0	41.0
19	37.775326346754916	39.0	37.0	41.0	33.0	41.0
20	37.53660349097896	39.0	35.0	41.0	33.0	41.0
21	37.40883901317709	39.0	35.0	41.0	33.0	41.0
22	37.480575617823185	39.0	35.0	41.0	33.0	41.0
23	37.42251809454502	39.0	35.0	41.0	33.0	41.0
24	37.35226775017351	39.0	35.0	41.0	33.0	41.0
25	37.24955792420265	39.0	35.0	41.0	33.0	41.0
26	37.223319750993646	39.0	35.0	41.0	33.0	41.0
27	37.1990630977168	39.0	35.0	41.0	33.0	41.0
28	37.0889258207882	39.0	35.0	41.0	33.0	41.0
29	36.971908333164876	39.0	35.0	41.0	32.0	41.0
30	36.791403755502486	39.0	35.0	41.0	32.0	41.0
31	36.566597744234244	39.0	35.0	41.0	31.0	41.0
32	36.3343320347808	39.0	35.0	41.0	30.0	41.0
33	35.9459579784935	39.0	35.0	41.0	29.0	41.0
34	35.55224772801437	38.0	35.0	41.0	26.0	41.0
35	35.27900012417589	38.0	35.0	40.0	24.0	41.0
36	35.08339951234491	38.0	34.0	40.0	24.0	41.0
37	35.04202872602274	38.0	34.0	40.0	23.0	41.0
38	34.89163632058172	38.0	34.0	40.0	23.0	41.0
39	34.821170352957935	38.0	34.0	40.0	23.0	41.0
40	34.69941733591696	38.0	34.0	40.0	22.0	41.0
41	34.535263546482696	38.0	34.0	40.0	22.0	41.0
42	34.399174470977016	38.0	34.0	40.0	21.0	41.0
43	34.24662246389995	38.0	34.0	40.0	21.0	41.0
44	34.16415330813236	37.0	33.0	40.0	20.0	41.0
45	34.158622667971954	37.0	33.0	40.0	20.0	41.0
46	33.98201326660564	37.0	33.0	40.0	20.0	41.0
47	33.91129942850212	37.0	33.0	40.0	20.0	41.0
48	33.80361919778524	37.0	33.0	40.0	20.0	41.0
49	33.84370827714134	37.0	33.0	40.0	20.0	41.0
50	33.66154080140617	36.0	33.0	40.0	20.0	41.0
51	32.663487186299456	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	30.0
10	40.0
11	47.0
12	33.0
13	38.0
14	50.0
15	93.0
16	172.0
17	373.0
18	705.0
19	1445.0
20	2527.0
21	4117.0
22	6180.0
23	9631.0
24	15974.0
25	27568.0
26	40800.0
27	45971.0
28	44801.0
29	43847.0
30	45936.0
31	53147.0
32	64320.0
33	86373.0
34	141945.0
35	160213.0
36	174035.0
37	242660.0
38	392280.0
39	472172.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.762593023625186	4.17505335231588	50.93988635499481	25.12246726906413
2	28.06187424736415	3.7693639787880624	46.96818305644035	21.20057871740744
3	18.112545711648178	4.034465066626622	55.82534131524408	22.027647906481114
4	16.570454416378126	4.365023213190752	55.11595044130571	23.948571929125407
5	16.2221843598059	6.927907713248027	54.13881131906562	22.711096607880453
6	19.26290538855984	6.557160857834006	56.54825677263972	17.631676980966436
7	73.92421805286428	1.8206688363756427	18.939085468629223	5.316027642130859
8	77.2558379514251	2.0750368917908184	16.225986644834812	4.443138511949282
9	71.53002986959606	4.2978815978068035	18.549182797499924	5.622905735097208
10	35.49558212983793	22.88547228711776	25.12713589751735	16.491809685526963
11	29.18638801211726	21.39353265007715	31.24611950341195	18.173959834393642
12	24.5315247933049	19.180458372679766	35.65277533115977	20.635241502855564
13	22.643810601925786	18.63807925887208	38.24530802840451	20.472802110797623
14	19.859334706006358	19.82362210484873	39.8702795112668	20.446763677878113
15	19.786946899886317	19.446570194513352	40.04123794699711	20.725244958603223
16	22.087425602758437	20.010752284499482	37.77892648498483	20.12289562775726
17	21.516409025758314	20.563623779779352	36.78542309806333	21.134544096398997
18	22.70397333972502	20.217712102528857	36.19082272784591	20.887491829900206
19	21.8850381528018	23.828872146000045	34.23620757203405	20.049882129164104
20	23.007386058994136	25.02105695822973	32.60074370769958	19.37081327507655
21	22.602514898700388	25.240578755911592	33.113185843178364	19.043720502209656
22	20.919979708311796	22.015711619301747	35.02405065606262	22.040258016323836
23	20.32783397779658	23.194419978264406	34.11482323225031	22.362922811688705
24	20.6985808332106	23.13493106312852	34.503955820335776	21.662532283325103
25	22.4548033448557	23.01917795560279	32.47035902234107	22.05565967720044
26	20.552409445453574	22.58648754535067	33.295262352853975	23.565840656341777
27	20.326630723040594	20.89042777150481	34.89178889328478	23.891152612169815
28	19.90578033958737	22.438872251886462	34.152075999495594	23.50327140903057
29	22.14575939332858	21.716149315251783	32.91994312936721	23.218148162052426
30	24.63712243069012	19.81977168962958	34.72891632951469	20.814189550165615
31	23.942266874204048	21.373895532459482	33.81603101124418	20.867806582092296
32	24.706863076347	20.725341218983704	33.089505789580585	21.47828991508872
33	24.90626645450879	20.54013624694253	32.20073369662001	22.352863601928675
34	22.35950556818171	21.361911115089875	34.49534051628292	21.783242800445493
35	22.295925586875477	23.831615566843688	33.0490764297795	20.82338241650134
36	23.305841368668595	25.26671344921158	30.896983103415415	20.53046207870441
37	23.51684412267808	24.633753317373362	31.27389062318008	20.575511936768482
38	23.17983653062187	24.707585029200587	31.464197395386623	20.648381044790916
39	23.866558084957486	23.02524235957295	29.611762633452987	23.496436922016578
40	24.216945869900243	22.7088344889392	31.27976250638928	21.79445713477127
41	22.130405862642213	24.18667198023967	30.633759092996193	23.049163064121927
42	22.84302145932662	24.367400844588577	30.540579044692734	22.248998651392068
43	22.400416229885188	22.154278437000947	32.313647122921616	23.131658210192242
44	23.502741976937937	22.33967592980308	30.991655187616296	23.165926905642685
45	23.14282441432778	21.63350977861075	30.272878926581242	24.950786880480223
46	22.56121919547499	22.216125731458565	30.859730336170127	24.362924736896314
47	21.29611714503263	23.26680778438445	32.31422468520449	23.12285038537843
48	21.447245942384313	22.94549063434628	32.27966720861261	23.327596214656797
49	22.953817157257696	21.71740070019801	31.795429364614108	23.53335277793019
50	21.20125254007079	21.333658693419352	32.45880777668362	25.00628098982624
51	20.4760749637339	20.9780247177405	32.52773020910642	26.018170109419174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426.0
1	1356.0
2	2286.0
3	33361.0
4	64436.0
5	41902.0
6	19368.0
7	18931.5
8	18495.0
9	18487.5
10	18480.0
11	18314.0
12	18148.0
13	17424.5
14	16701.0
15	15749.0
16	14797.0
17	13842.0
18	12887.0
19	12135.5
20	11384.0
21	11018.0
22	10652.0
23	10423.0
24	10194.0
25	10920.5
26	12471.0
27	13295.0
28	15364.5
29	17434.0
30	19387.5
31	21341.0
32	25598.0
33	29855.0
34	34733.0
35	39611.0
36	42550.5
37	45490.0
38	49977.0
39	54464.0
40	60084.5
41	65705.0
42	74732.5
43	83760.0
44	97808.0
45	111856.0
46	139551.5
47	167247.0
48	187515.5
49	207784.0
50	208719.0
51	209654.0
52	189155.5
53	168657.0
54	151806.5
55	134956.0
56	122258.0
57	109560.0
58	101917.0
59	94274.0
60	86412.0
61	78550.0
62	69871.0
63	61192.0
64	53603.0
65	46014.0
66	37756.0
67	29498.0
68	24504.0
69	19510.0
70	16611.0
71	13712.0
72	11517.5
73	9323.0
74	8207.5
75	5451.5
76	3811.0
77	3054.0
78	2297.0
79	1667.0
80	1037.0
81	750.0
82	463.0
83	339.0
84	215.0
85	146.0
86	77.0
87	58.0
88	39.0
89	25.5
90	12.0
91	8.5
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2077698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.41629412634605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72023848431562	25.71593704866928
2	8.170474222345774	5.623948879759613
3	3.1303272520861847	3.2320279025854424
4	1.8798203519183754	2.587858005452566
5	1.345975394917714	2.316174253915641
6	1.1143333667065551	2.301073494202454
7	0.9628612443256486	2.319668105129975
8	0.8554426386890589	2.355293236907218
9	0.8186892238836533	2.535862421452481
>10	6.80257110754767	38.65598716691557
>50	0.12126167063215344	2.892345907332162
>100	0.07133989576695186	4.555617057714118
>500	0.004254349062525554	0.9227320883650558
>1k	0.002127174531262777	1.8510280960188883
>5k	1.4181163541751848E-4	0.2442755793011268
>10k+	1.4181163541751848E-4	1.8901707562785295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38728	1.8639860075910935	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTT	5005	0.24089160214814664	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	4399	0.21172470686307635	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	4100	0.19733377998149876	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	3965	0.1908362042991811	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	3680	0.17711910008095497	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	3378	0.16258378262865922	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	2911	0.14010698378686412	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	2335	0.11238399420897552	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9252076095755975E-4	0.0	0.0	0.17336494524228258	0.0
2	1.9252076095755975E-4	0.0	0.0	0.5737118676535281	0.0
3	1.9252076095755975E-4	0.0	0.0	1.0292159880791145	0.0
4	1.9252076095755975E-4	0.0	0.0	1.540888040514069	0.0
5	1.9252076095755975E-4	0.0	0.0	2.5588896942674055	0.0
6	1.9252076095755975E-4	0.0	0.0	4.326759711950437	0.0
7	1.9252076095755975E-4	0.0	0.0	5.8396359817451815	0.0
8	1.9252076095755975E-4	0.0	0.0	7.698038887268506	0.0
9	1.9252076095755975E-4	0.0	0.0	9.189064050694567	0.0
10	1.9252076095755975E-4	0.0	0.0	10.780007489057601	0.0
11	2.406509511969497E-4	0.0	0.0	12.558610539164016	0.0
12	2.406509511969497E-4	0.0	0.0	13.903801226164726	0.0
13	2.406509511969497E-4	0.0	0.0	14.4675501444387	0.0
14	2.406509511969497E-4	0.0	0.0	14.779481907380188	0.0
15	2.8878114143633964E-4	0.0	0.0	15.031828494805309	0.0
16	3.369113316757296E-4	0.0	0.0	15.407484629623747	0.0
17	4.3317171215450945E-4	0.0	0.0	15.90105973052869	0.0
18	4.3317171215450945E-4	0.0	0.0	16.45272797105258	0.0
19	4.813019023938994E-4	0.0	0.0	16.83372655698759	0.0
20	6.738226633514592E-4	0.0	0.0	17.24716489114395	0.0
21	6.738226633514592E-4	0.0	0.0	17.739777388244104	0.0
22	6.738226633514592E-4	0.0	0.0	18.291301238197274	0.0
23	6.738226633514592E-4	0.0	0.0	18.818038040177157	0.0
24	6.738226633514592E-4	0.0	0.0	19.257033505350634	0.0
25	6.738226633514592E-4	0.0	0.0	19.65574400129374	0.0
26	6.738226633514592E-4	0.0	0.0	19.998190304847	0.0
27	6.738226633514592E-4	0.0	0.0	20.377167422791956	0.0
28	6.738226633514592E-4	0.0	0.0	20.74699980459143	0.0
29	6.738226633514592E-4	0.0	0.0	21.17126743155165	0.0
30	6.738226633514592E-4	0.0	0.0	21.67307279498753	0.0
31	6.738226633514592E-4	0.0	0.0	22.056526020624748	0.0
32	6.738226633514592E-4	0.0	0.0	22.467365324508183	0.0
33	6.738226633514592E-4	0.0	0.0	22.865546388358656	0.0
34	6.738226633514592E-4	0.0	0.0	23.264738186204156	0.0
35	6.738226633514592E-4	0.0	0.0	23.704359343850744	0.0
36	6.738226633514592E-4	0.0	0.0	24.11341783069532	0.0
37	6.738226633514592E-4	0.0	0.0	24.528155679988142	0.0
38	6.738226633514592E-4	0.0	0.0	25.06129379726986	0.0
39	6.738226633514592E-4	0.0	0.0	25.79364277195242	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21405	0.0	43.738613	1
CCATACG	125	0.0	39.600002	2
GTTTTTT	24520	0.0	38.65008	2
CGTTATT	580	0.0	38.405174	1
GAATCGT	30	1.1402393E-4	37.499996	9
TTGGGAC	3240	0.0	36.52778	5
CGTTTTC	690	0.0	36.521736	1
TGGGCGA	3420	0.0	35.921055	6
CGTTTAT	270	0.0	35.833332	1
TTTGGGA	8685	0.0	35.72539	4
GGAACAT	1790	0.0	35.446926	8
CACGACC	1590	0.0	35.377357	27
TATTGCG	115	0.0	35.21739	1
CGACCAA	1595	0.0	35.125393	29
CTTGGGA	4495	0.0	35.038933	4
TGGGAAC	3055	0.0	34.762684	6
TTACGGG	525	0.0	34.714287	3
TTGGGAG	4805	0.0	34.69823	5
ACAACGA	240	0.0	34.6875	13
GACACGA	1630	0.0	34.64724	25
>>END_MODULE