SRR2933175.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933175.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421222
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	7553	0.5314440671478489	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	6498	0.4572121737490695	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	5768	0.40584792523617	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3783	0.266179386471642	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	3337	0.23479794148978833	RNA PCR Primer, Index 33 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	2921	0.20552735603586209	TruSeq Adapter, Index 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	2352	0.16549138698950622	RNA PCR Primer, Index 33 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCGAACT	1878	0.13213980644825368	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1872	0.13171763454266822	Illumina Paired End PCR Primer 2 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1636	0.11511220625630621	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTC	1471	0.10350247885270562	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	1463	0.10293958297859167	RNA PCR Primer, Index 33 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTT	20	7.033851E-4	45.0	18
ATCGCTA	20	7.033851E-4	45.0	24
TATACGT	30	2.165867E-6	44.999996	13
CGATGAA	495	0.0	43.181816	19
TTACGGG	345	0.0	41.739132	3
CGGTCTA	60	3.6379788E-12	41.249996	31
TATTGCG	55	6.184564E-11	40.909092	1
CGGGTAC	95	0.0	40.263157	6
TCCGATA	45	1.9288564E-8	40.000004	14
TACGGGT	90	0.0	40.000004	4
CGTTAGG	155	0.0	39.19355	2
TTTGGGC	3835	0.0	38.898308	4
CCGATGA	550	0.0	38.454544	18
TCGTTTG	430	0.0	38.197674	1
CGTTTGG	965	0.0	38.00518	2
TTTGGGA	7745	0.0	37.940605	4
TTGGGAC	3010	0.0	37.599667	5
CGACGGT	60	1.5643309E-10	37.499996	28
ATACGTC	30	1.140058E-4	37.499996	14
TGGGCGA	2710	0.0	37.44465	6